A Myosin XI Tail Domain Homologous to the Yeast Myosin Vacuole-Binding Domain Interacts with Plastids and Stromules in Nicotiana benthamiana
A Myosin XI Tail Domain Homologous to the Yeast Myosin Vacuole-Binding Domain Interacts with Plastids and Stromules in Nicotiana benthamiana作者机构：Department of Molecular Biology and Genetics Biotechnology Building Cornell University Ithaca NY 14853 USA
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2009年第2卷第6期
页 面：1351-1358页
核心收录：
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 090401[农学-植物病理学] 0902[农学-园艺学] 090402[农学-农业昆虫与害虫防治]
基 金：grants from the Chemical Sciences Geosciences and Biosciences Division Office of Basic Energy Scien-ces Office of Science US Department of Energy to M.R.H. by Cornell University and by a fellowship from the Deutscher Akadem
主 题：Chloroplast biology cytoskeleton dynamics mitochondria gene silencing fluorescent protein.
摘 要：The actin cytoskeleton plays a role in mobility of many different organelles in plant cells, including chloroplasts, mitochondria, Golgi, and peroxisomes. While progress has been made in identifying the myosin motors involved in trafficking of various plant organelles, not all of the cargoes mobilized by different members of the myosin XI family have yet been identified. The involvement of myosins in chloroplast positioning and mitochondrial movement was demonstrated by expression of a virus-induced gene silencing （VIGS） construct in tobacco. When VIGS with two different conserved sequences from a myosin Xl motor was performed in plants with either GFP-labeled plastids or mitochondria, chloroplast positioning in the dark was abnormal, and mitochondrial movement ceased. Because these and prior obser- vations have implicated a role for myosins and the actin cytoskeleton in plastid and stromule movement, we searched for myosin tail domains that could associate with plastids and stromules. While a yellow fluorescent protein （YFP） fusion with the entire tail region of myosin XI-F was usually found only in the cytoplasm, we observed that an Arabidopsis or Nicotiana benthamiana YFP：：myosin XI-F tail domain homologous to the yeast myo2p vacuole-binding domain associated with plastids and stromules after transient expression in N. benthamiana. Taken together, these observations implicate myosin motor proteins in dynamics of plastids and stromules.
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