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First molecular identification of Cryptosporidium by 18S rRNA in goats and association with farm management in Terengganu

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Asian Pacific Journal of Tropical Biomedicine 2017年第5期7卷 385-388页

作者： Afzan Mat Yusof najat hashim Muhammad Lokman Md Isa Department of Basic Medical Sciences Kulliyyah of NursingInternational Islamic University MalaysiaJalan Hospital Campus25100 KuantanPahangMalaysia Integrated Cellular and Molecular Biology Cluster(iMolec) International Islamic University MalaysiaJalan Sultan Ahmad ShahBandar Indera Mahkota25200 KuantanPahangMalaysia Department of Biomedical Science Kulliyyah of Allied Health SciencesInternational Islamic University MalaysiaJalan Sultan Ahmad ShahBandar Indera Mahkota25200 KuantanPahangMalaysia

Objective:To identify the prevalence of Cryptosporidium from goats in three types of farm management systems in Terengganu,Malaysia and to determine the Cryptosporidium species infecting goats by using 18 S r ***:A total of 478 fecal samples were randomly collected from goats in three farms;199 samples were collected from intensive farm,179 samples from semi-intensive farm and 100 samples from extensive *** samples were processed by using formolether concentration technique and stained by using modified Ziehl–*** samples were performed by using nested PCR analysis by using 18 S r ***:Out of 478 goats,207(43.3%) were found to be infected with *** reared under the intensive farm management system reported the highest prevalence of infection(49.7%),followed by intensive farm management system(41%) and the lowest prevalence was reported in the goats reared under semi-intensive management system(37.4%).Conclusions:The identified species found in goat was Cryptosporidium *** study on the zoonotic transmission of Cryptosporidium parvum in goats needs to be done in order to find the source of transmission of this parasite.

关键词： Cryptosporidium Goat 18S rRNA gene Nested PCR Terengganu

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