First molecular identification of Cryptosporidium by 18S rRNA in goats and association with farm management in Terengganu

作     者：Afzan Mat Yusof Najat Hashim Muhammad Lokman Md Isa 

作者机构：Department of Basic Medical SciencesKulliyyah of NursingInternational Islamic University MalaysiaJalan Hospital Campus25100 KuantanPahangMalaysia Integrated Cellular and Molecular Biology Cluster(iMolec)International Islamic University MalaysiaJalan Sultan Ahmad ShahBandar Indera Mahkota25200 KuantanPahangMalaysia Department of Biomedical ScienceKulliyyah of Allied Health SciencesInternational Islamic University MalaysiaJalan Sultan Ahmad ShahBandar Indera Mahkota25200 KuantanPahangMalaysia 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2017年第7卷第5期

页      面：385-388页

核心收录：

学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：Supported by IIUM Research Initiative Grant RIGS grant no.16-301-0465 

主　　题：Cryptosporidium Goat 18S rRNA gene Nested PCR Terengganu 

摘      要：Objective:To identify the prevalence of Cryptosporidium from goats in three types of farm management systems in Terengganu,Malaysia and to determine the Cryptosporidium species infecting goats by using 18 S r ***:A total of 478 fecal samples were randomly collected from goats in three farms;199 samples were collected from intensive farm,179 samples from semi-intensive farm and 100 samples from extensive *** samples were processed by using formolether concentration technique and stained by using modified Ziehl–*** samples were performed by using nested PCR analysis by using 18 S r ***:Out of 478 goats,207(43.3%) were found to be infected with *** reared under the intensive farm management system reported the highest prevalence of infection(49.7%),followed by intensive farm management system(41%) and the lowest prevalence was reported in the goats reared under semi-intensive management system(37.4%).Conclusions:The identified species found in goat was Cryptosporidium *** study on the zoonotic transmission of Cryptosporidium parvum in goats needs to be done in order to find the source of transmission of this parasite.