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30th International Symposium on Capillary Chromatography、4th GC×GC Symposium、16th National Symposium and Exhibition on Chromatography,i.e. 1st Dalian International Symposium and Exhibition on Chromatography

作者： Baba Y.~(1-5 *) 1.Department of Applied Chemistry Graduate School of Engineering nagoya university 2.MEXT Innovative research center for Preventive Medical Engineering nagoya university 3.plasma nanotechnology research center nagoya university 4.Health Technology research center National Institute of Advanced Industrial Science and Technology (AlST) 5.Institute for Molecular Science National Institute for Natural Sciences

＜正＞This lecture will describe the recent development of nanobiotechnologies based on the nanofabrication, molecular nanotechnoiogy,and nanomaterials for bioanalysis and biomedical *** technologies allow us to develop novel methods to analyze several biomolecules,including DNA,RNA,protein,peptide, polysaccharide,and *** of nano-pillar,which is 200 nm wide and 4000 nm tall,is successfully applicable to fast separation of DNA within 10-25 ***-ball,fabricated by molecular nanotechnology,of which diameter is 50 nm,is developed and successfully applied for fast separation of DNA fragments from 100 bp to 15 *** cellulose,which is 50 nm diameter biofiber,is found to be useful high sensitive detection of biomolecule based on the optical confined effect as well as fast analysis of *** nano-biodevice is extremely useful for fast separation of protein samples from several kDa to 200 kDa within 15 seconds. Utilizing this technique,complex protein mixture extracted from human T cell line,lymphoblastic Jurkat cells, were separated within 15s with high *** methods we have taken appear suitable for biomedical and clinical applications,including SNPs analysis,mutation analysis,haplotyping,DNA diagnosis,protein expression analysis,immunoassay,protein disease marker detection,point-of-care *** methods are also applicable to molecular imaging of cancer cell and possible photodynamic therapy of cancer.

关键词： Nanotechnoiogy Biodevice Protein DNA Cell

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DISSOLUTION THEORY OF GOLD IN ALKALINE THIOUREA SOLUTION(Ⅱ)——Electrochemical Study on Au-Fe Mix Anode in Alkaline Thiourea Solution Containing Na_2SO_3

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中国有色金属学会会刊：英文版 1999年第2期9卷 189-194页

作者： Chai Liyuan1 and Masazumi Okido2 1 Department of Nonferrous Metallurgy,Central South university of Technology, Changsha 410083, P. R. China2 center for Integrated research in Science and Engineering,nagoya university, nagoya 464, Japan 中南工业大学有色冶金系湖南长沙 nagoya Univerisity 日本

１ＩＮＴＲＯＤＵＣＴＩＯＮＭａｎｙｓｔｕｄｉｅｓｏｎｔｈｅｅｘｔｒａｃｔｉｏｎｏｆｇｏｌｄｆｒｏｍｏｒｅｏｒｓｃｒａｐｗｉｔｈｔｈｉｏｕｒｅａｗｅｒｅｒｅｐｏｒｔｅｄ［１－３］，ｂｕｔａｌｔｈｏｓｅｗｅｒｅｌｉｍｉｔ... 详细信息

１ＩＮＴＲＯＤＵＣＴＩＯＮＭａｎｙｓｔｕｄｉｅｓｏｎｔｈｅｅｘｔｒａｃｔｉｏｎｏｆｇｏｌｄｆｒｏｍｏｒｅｏｒｓｃｒａｐｗｉｔｈｔｈｉｏｕｒｅａｗｅｒｅｒｅｐｏｒｔｅｄ［１－３］，ｂｕｔａｌｔｈｏｓｅｗｅｒｅｌｉｍｉｔｅｄｏｎｌｙｉｎａｃｉｄｉｃｍｅｄ...

关键词： alkaline thioureasodium sulfitemix anode of gold and iron

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Dissolution theory of gold in alkaline thiourea solution(Ⅲ) ——Thermodynamics on dissolution of gold in alkaline thiourea solution containing Na _2SO_3

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中国有色金属学会会刊：英文版 1999年第3期9卷 646-650页

作者： Chai Liyuan(柴立元) 1, Masazumi Okido 2 1. Department of Nonferrous Metallurgy, Central South university of Technology, Changsha 410083, P.R.China 2. center for Integrated research of Science and Engineering, nagoya university,nagoya 464, Japan 中南工业大学有色冶金系湖南长沙 nagoya university 日本

The oxidants of gold were investigated in an alkaline thiourea solution containing Na 2CO 3, in which Na 2S 2O 8 is a proper oxidant for dissolving gold because of in such homogeneous sulfur system coexisting complex agent, oxidant and stabilizing agent. The thermodynamic analyses were conducted on the dissolving of gold in the alkaline thiourea solution containing Na 2SO 3 by the oxidants oxygen or Na 2S 2O 8. The results show that the possibility of gold dissolution reduces with increasing pH value, while oxygen acts as oxidant; and when Na 2S 2O 8 acts as the oxidant of gold in the alkaline thiourea solution of pH 12.5, decomposition potential of thiourea decreases from the standard value 0.42 V to -0.32 V, also the dissolution trend of gold enhances with shifting the mix potential of the solution to the positive direction.

关键词： thermodynamic analysis gold dissolution thiourea leaching Document code:A

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Study on rare earth/alkaline earth oxide-doped CeO_2 solid electrolyte

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Rare Metals 2007年第4期26卷 311-316页

作者： YAN Kai ZHEN Qiang Song Xiwen School of Materials Science and Engineering Shanghai University Shanghai 200072 China research center of Nanoscience and nanotechnology Shanghai Univeesity Shanghai 200444 China School of Materials Science and Engineering Inner Mongolia University of Science and Technology B aotou 014010 China

Five types of rare earth/alkaline earth oxide-doped CeO2 superfine-powders were synthesized by a low-temperature combustion technique. The relevant solid electrolyte materials were also sintered by pressureless sintering at different temperatures. The results of X-ray diffraction and transmission electron microscopy showed that the grain size of the powders was approximately 20-30 nm, and rare earth/alkaline earth oxides were completely dissolved into ceria-based solid solution with fluorite structure. The electrical conductivities of the SmzO3-CeO2 system were measured by the ac impedance technique in air at temperatures ranging from 513-900℃. The results indicated that the ionic conductivities of Srno.2oCe0.8Ol.875 solid electrolyte increase with increasing sintering temperature, and the relationship between the conductivities and measuring temperature obeys the An＇henius equation. Then the SmzO3-CeO2 material was further doped with other rare earth/alkaline earth oxide, and the conductivities improve with the effective index.

关键词： doped ceria rare earth/alkaline earth oxide solid electrolyte conductivity effective index

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Studies of Atomic Structure and Physical Properties of Metal Clusters in MgO by HREM and Nano-probe Methods

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Journal of Materials Science & Technology 1997年第4期13卷 265-270页

作者： Nobuo Tanaka (Dept. of Applied Physics, School of Engineering, nagoya university, nagoya, 464-01, Japan) Dept. of Applied Physics School of Engineering Nagoya University Nagoya 464-01 Japan

Nanometer-sized metal clusters were prepared inside single crystalline MgO films by vacuum co-deposition of metals and MgO. The atomic structure was studied by high-resolution electron microscopy (HREM) and nm-area electron diffraction. The size of the clusters is ranging from 1 nm to 3 nm without those larger than 5 nm, and most of them have definite epitaxial orientations with the MgO matrix films. The character of the composite films is very much useful for the studies of various kinds of physical properties with anisotroPy. The physical properties such as electric transport, magnetic, optical absorption, sintering and catalytic ones were thus measured on the same samples analyzed by HREM by using high sensitivity apparatus with interest of clarifying the retationship between the atomic structure and physical properties

关键词： Figure Nano Studies of Atomic Structure and Physical Properties of Metal Clusters in MgO by HREM and Nano-probe Methods HREM MgO

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Protective effects of curcumin on methylglyoxal-induced oxidative DNA damage and cell injury in human mononuclear cells

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Acta Pharmacologica Sinica 2006年第9期27卷 1192-1198页

作者： Wen-hsiung CHAN~1 Hsin-jung WU Department of Bioscience Technology and center for nanotechnology,Chung Yuan Christian university,Chung Li 32023,Taiwan,China Department of Bioscience Technology and center for nanotechnology Chung Yuan Christian University Chung Li China

Aim:To examine the effect of curcumin on oxidative DNA damage and cell apoptosisand injury caused by the reaction of methylglyoxal(MG)with amino ***:We used DNA strand breaks to examine the effect of curcumin on oxidative *** addition,reactive oxygen species(ROS)formation occurs in MG-treatedmononuclear cells,so the effect of curcumin on ROS generation was measuredusing 2′,7′-dichlorofluorescin diacetate(DCF-DA)as the detection ***,the impact effects of curcumin on MG-induced cell apoptosis and ROSinjury were analyzed by TUNEL and ELISA *** collagen Ⅰ attachmentability of mononuclear cells was examined by trypan blue ***:Ourresults revealed that curcumin prevented MG/lysine-induced oxidative stress andDNA *** also inhibited MG-induced apoptosis and generation ofROS in mononuclear ***-treated mononuclear cells displayed a lowerdegree of attachment to collagen(the major component of the vessel wall subendo-thelium),whereas cells pretreated with curcumin before MG treatment exhibitedrestored affinities for ***:These results demonstrated thatoxidative stress plays a role in MG-induced cell injury and alterations in attach-ment ability,and that curcumin blocks these effects by virtue of its antioxidantproperties.

关键词： curcumin methylglyoxal DNA damage oxidative stress

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DETECTION OF TRANSIENT SIGNAL USING THE DISCRETE WAVELET TRANSFORM

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国防科技大学学报 1995年第3期17卷 114-118,125页

作者： Liu Zheng(Department of Information Electronics,Faculty of Engineering,nagoya university, nagoya, 464,Japan)

ＤＥＴＥＣＴＩＯＮＯＦＴＲＡＮＳＩＥＮＴＳＩＧＮＡＬＵＳＩＮＧＴＨＥＤＩＳＣＲＥＴＥＷＡＶＥＬＥＴＴＲＡＮＳＦＯＲＭＬｉｕＺｈｅｎｇ（ＤｅｐａｒｔｍｅｎｔｏｆＩｎｆｏｒｍａｔｉｏｎＥｌｅｃｔｒｏｎｉｃｓ，Ｆａｃｕｌｔ... 详细信息

ＤＥＴＥＣＴＩＯＮＯＦＴＲＡＮＳＩＥＮＴＳＩＧＮＡＬＵＳＩＮＧＴＨＥＤＩＳＣＲＥＴＥＷＡＶＥＬＥＴＴＲＡＮＳＦＯＲＭＬｉｕＺｈｅｎｇ（ＤｅｐａｒｔｍｅｎｔｏｆＩｎｆｏｒｍａｔｉｏｎＥｌｅｃｔｒｏｎｉｃｓ，ＦａｃｕｌｔｙｏｆＥｎｇｉｎｅｅｒｉｎｇ，Ｎａ...

关键词：信号检测瞬态信号 DWT

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Quantitative detection of ING1 mRNA under different gene regulation based on molecular beacon

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Chinese Science Bulletin 2006年第17期51卷 2059-2064页

作者： LIU Bin1,2,3,4, WANG Kemin1,2,3,4, XIAO Zhiqiang5,6, WANG Wei1,3,4, TAN Weihong1,2,3,4, SUN Yi5,6, TANG Hongxing1,3,4 & YANG Xiaohai1,3,41. State Key Laboratory of Chemo/Biosensing and Chemometrics, Hu- nan university, Changsha 410082, China 2. Life Science and Biotechnology Institute, Hunan university, Chang- sha 410082, China 3. Chemistry and Chemical Engineer College, Hunan university, Changsha 410082, China 4. Hunan Engineering research center for Bio-nanotechnology, Hunan university, Changsha 410082, China 5. Medical Experiment research center, Xiangya Hospital, Central South university, Changsha 410008, China 6. Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Changsha 410008, China State Key Laboratory of Chemo/Biosensing and Chemometrics Hunan University Changsha China Life Science and Biotechnology Institute Hunan University Changsha China Chemistry and Chemical Engineer College Hunan University Changsha China Hunan Engineering research center for Bio-nanotechnology Hunan University Changsha China Medical Experiment research center Xiangya Hospital Central South University Changsha China Key Laboratory of Cancer Proteomics of Chinese Ministry of Health Changsha China

The effects of drug treatment, gene transfection, and p53 RNA interference (RNAi) on level of ING1 mRNA in tumor cells were quantitatively detected with the help of ING1 molecular beacon (MB)/cRNA standard curve based on MB detection technology. Results showed that the level of ING1 mRNA could be upregulated by 5-FU treatment or ING1 transfection in low expression cell line of MCF-7 and be inhibited by silencing p53 with RNAi technology in normal expression cell line of CNE2. The level of ING1 varied from 7.7×10?16 to 53.4× 10?16 mol/μg total RNA of tumor cells. The results not only provided evidence for the regulation effects of gene expression but also were applied to investigat- ing the interaction of multigenes on signal transduc- tion pathway.

关键词： ING1 RT-PCR 定量分析分子信号 mRNA

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Effect of genistein on mouse blastocyst development in vitro

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Acta Pharmacologica Sinica 2007年第2期28卷 238-245页

作者： Wen-hsiung CHAN~1 Hsiang-yu LU Nion-heng SHIAODepartment of Bioscience Technology and center for nanotechnology,Chung Yuan Christian university Chung Li 32023,Taiwan,China Department of Bioscience Technology and center for nanotechnology Chung Yuan Christian University Chung Li China

Aim:To examine the cytotoxic effects of genistein,an isoflavone compound,onearly postimplantation embryonic development in ***:Mouse blasto-cysts were incubated in medium with or without genistein(25 or 50 μmol/L)ordaidzein(50 μtmol/L)for 24 *** proliferation and growth was investigated bydual differential staining,apoptosis was analyzed by terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling(TUNEL)assay,and apoptotic ornecrotic cells were visualized by Annexin-V and propidium iodide(PI)*** and postimplantation development of embryos were measured by invitro development ***:TUNEL staining and Annexin-V/PI stainingshowed that genistein dose-dependently increased apoptosis in mouseblastocysts,while daidzein,another soy isoflavone,had no such *** of the blastocysts with genistein caused fewer cells than the controlgroup and this effect was primary in the inner cell *** genistein-pretreatedblastocysts showed normal levels of implantation on culture dishes in vitro,butsignificantly fewer genistein-pretreated embryos reached the later stages of em-bryonic development versus the controls,with many of the former embryos dyingat relatively early stages of *** addition,genistein treatment de-creased the development of morulas into blastocysts,and dietary genistein wasfound to induce cell apoptosis and decrease cell proliferation in an animal assaymodel of ***:Our results collectively indicate thatgenistein treatment of mouse blastocysts induces apoptosis,decreases cellnumbers,retards early postimplantation blastocyst development,and increasesearly-stage blastocyst death in vitro,while dietary genistein appears to nega-tively affect mouse embryonic development in vivo by inducing cell apoptosisand inhibiting cell *** novel findings provide important newinsights into the effect of genistein on mouse blastocysts.

关键词： genistein blastocyst development implantation

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DISSOLUTION THEORY OF GOLD IN ALKALINE THIOUREA SOLUTION(Ⅰ) Anodic Behavior on Gold in Alkaline Thiourea Solution Containing Na_2SO_3

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中国有色金属学会会刊：英文版 1999年第1期9卷 147-153页

作者： Chai Liyuan and Masazumi Okido Department of Nonferrous Metallurgy, Central South university of Technology, Changsha 410083, P. R.China center for Integrated research in Science and Engineering, nagoya university, nagoya 464, Japan

ＩＮＴＲＯＤＵＣＴＩＯＮＳｉｎｃｅｔｈｉｏｕｒｅａｗａｓｕｔｉｌｉｚｅｄａｓｔｈｅｌｉｘｉｖｉａｎｔｏｆｇｏｌｄｅａｒｌｙｉｎ１９３２［１］，ｅｘｔｒａｃｔｉｎｇｇｏｌｄｆｒｏｍｏｒｅｏｒｓｃｒａｐｂｙａｃｉｄｉｃｔ... 详细信息

ＩＮＴＲＯＤＵＣＴＩＯＮＳｉｎｃｅｔｈｉｏｕｒｅａｗａｓｕｔｉｌｉｚｅｄａｓｔｈｅｌｉｘｉｖｉａｎｔｏｆｇｏｌｄｅａｒｌｙｉｎ１９３２［１］，ｅｘｔｒａｃｔｉｎｇｇｏｌｄｆｒｏｍｏｒｅｏｒｓｃｒａｐｂｙａｃｉｄｉｃｔｈｉｏｕｒｅａｓｏｌｕｔｉｏｎｈａ...

关键词： alkaline thiourea sodium sulfite polarization behavior gold

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