Protective effects of curcumin on methylglyoxal-induced oxidative DNA damage and cell injury in human mononuclear cells
Protective effects of curcumin on methylglyoxal-induced oxidative DNA damage and cell injury in human mononuclear cells作者机构：Department of Bioscience Technology and Center for Nanotechnology Chung Yuan Christian University Chung Li China
出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))
年 卷 期：2006年第27卷第9期
页 面：1192-1198页
核心收录：
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
主 题：curcumin methylglyoxal DNA damage oxidative stress
摘 要：Aim:To examine the effect of curcumin on oxidative DNA damage and cell apoptosisand injury caused by the reaction of methylglyoxal(MG)with amino ***:We used DNA strand breaks to examine the effect of curcumin on oxidative *** addition,reactive oxygen species(ROS)formation occurs in MG-treatedmononuclear cells,so the effect of curcumin on ROS generation was measuredusing 2′,7′-dichlorofluorescin diacetate(DCF-DA)as the detection ***,the impact effects of curcumin on MG-induced cell apoptosis and ROSinjury were analyzed by TUNEL and ELISA *** collagen Ⅰ attachmentability of mononuclear cells was examined by trypan blue ***:Ourresults revealed that curcumin prevented MG/lysine-induced oxidative stress andDNA *** also inhibited MG-induced apoptosis and generation ofROS in mononuclear ***-treated mononuclear cells displayed a lowerdegree of attachment to collagen(the major component of the vessel wall subendo-thelium),whereas cells pretreated with curcumin before MG treatment exhibitedrestored affinities for ***:These results demonstrated thatoxidative stress plays a role in MG-induced cell injury and alterations in attach-ment ability,and that curcumin blocks these effects by virtue of its antioxidantproperties.
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