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miRna-34家族在宫颈癌中作用机制的研究进展

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中国当代医药 2021年第11期28卷 36-40页

作者：杨世慧刘志强盖娜王志敏李明秀滨州医学院附属医院妇科山东滨州256600

宫颈癌是指原发于宫颈的恶性肿瘤,是威胁全球妇女健康最常见的恶性肿瘤之一,早期症状常不明显,尽管使用了先进的筛查手段和预防性疫苗,但是在发展中国家,其发病率及死亡率仍较高,因此需要寻找新的更有效的分子标记来进一步辅助宫颈癌的... 详细信息

宫颈癌是指原发于宫颈的恶性肿瘤,是威胁全球妇女健康最常见的恶性肿瘤之一,早期症状常不明显,尽管使用了先进的筛查手段和预防性疫苗,但是在发展中国家,其发病率及死亡率仍较高,因此需要寻找新的更有效的分子标记来进一步辅助宫颈癌的诊断、治疗和预后的评估。miRna-34家族在肿瘤细胞的增殖、周期调控、凋亡以及衰老等过程中发挥着重要作用,是癌症分子靶向治疗研究的热点。本文总结了miRna-34家族在恶性肿瘤中的分子作用机制,重点概述了宫颈癌中miRna-34的作用及研究进展。

关键词： microRna miR-34 p53 宫颈癌

护理本科实习生姑息护理认知与临终关怀态度现状及相关分析

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国际医药卫生导报 2024年第9期30卷 1543-1547页

作者：改娜雷清梅石盛文颜红波欧丽珊何钰熙关玉仙广州医科大学附属第二医院神经外科广州510260 广州医科大学附属第二医院内镜中心广州510260

目的调查广州市护理专业本科实习生的姑息护理认知与临终关怀态度现状并进行相关分析,为本科护理学生教学培训提供参考依据。方法于2021年6月至2022年4月对广州市3所三甲医院(广州医科大学附属第二医院、广州医科大学附属第一医院、广... 详细信息

目的调查广州市护理专业本科实习生的姑息护理认知与临终关怀态度现状并进行相关分析,为本科护理学生教学培训提供参考依据。方法于2021年6月至2022年4月对广州市3所三甲医院(广州医科大学附属第二医院、广州医科大学附属第一医院、广东药科大学附属第一医院)205名护理专业本科实习生进行便利抽样调查,其中男82例、女123例,年龄23岁13例。问卷内容包括一般人口学资料(性别、年龄、生源地、对照顾临终患者的感受、对姑息护理和临终关怀的理解、对临终患者护理的了解以及对其家属的态度)、中文版姑息护理知识问卷(PCQN)和中文版佛罗梅尔特临终关怀态度量表(FATCOD-B)。统计学方法采用t检验、方差分析、Pearson相关分析。结果本次调查研究回收的205份有效问卷中,实习生生源地来自城市62.0%(127/205),而来自农村仅38.0%(78/205);非独生子女53.2%(109/205),独生子女46.8%(96/205);护理专业不是自己自愿专业60.5%(124/205);护生中有55.1%(113/205)不了解姑息护理这一理念,仅有44.9%(92/205)知道姑息护理;63.4%(130/205)的护生不了解姑息护理和临终关怀的区别;43.4%(89/205)的护生表示了解姑息护理的内涵;有42.0%(86/205)在以往的实习和生活中照顾过终末期不可治愈的患者或者家属;52.7%(108/205)的护生在医院实习过程中没有护理过临终患者;而实习生在患者或者家属面前公开讨论死亡这个话题的仅有39.5%(81/205)。205名护理专业本科实习生姑息护理认知得分为(36.62±3.98)分,临终关怀态度得分为(99.38±8.93)分;姑息护理认知与临终关怀态度呈正相关(r=0.282,P<0.01)。结论护理本科实习生对姑息护理和临终关怀的认知仍有待提高,高校教师应注重护生对这两方面专业知识和专业技能的培养及训练,在本科学习中开展相关知识的理论学习和课程实践。

关键词：临终关怀姑息护理护理专业本科实习生

P2X7 receptor activation aggravates naDPH oxidase 2-induced oxidative stress after intracerebral hemorrhage

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Neural Regeneration Research 2021年第8期16卷 1582-1591页

作者： Hong Deng Ye Zhang gai-gai Li Hai-Han Yu Shuang Bai Guang-Yu Guo Wen-Liang Guo Yang Ma Jia-Hui Wang na Liu Chao Pan Zhou-Ping Tang Department of Neurology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Endocrinology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Oxidative stress is a crucial pathological process that contributes to secondary injury following intracerebral hemorrhage. P2X7 receptor(P2X7R), which is activated by the abnormal accumulation of extracellular ATP, plays an important role in the regulation of oxidative stress in the central nervous system, although the effects of activated P2X7R-associated oxidative stress after intracerebral hemorrhage remain unclear. Mouse models of intracerebral hemorrhage were established through the stereotactic injection of 0.075 U VII collagenase into the right basal ganglia. The results revealed that P2X7R expression peaked 24 hours after intracerebral hemorrhage, and P2X7R expressed primarily in neurons. The inhibition of P2X7R, using A438079(100 mg/kg, intraperitoneal), reduced nicotinamide adenine dinucleotide phosphate oxidase 2(NOX2) expression and malondialdehyde generation, increased superoxide dismutase and glutathione/oxidized glutathione levels, and alleviated neurological damage, brain edema, and apoptosis after intracellular hemorrhage. The P2X7R inhibitor A438079(100 mg/kg, intraperitoneal injection) inhibited the activation of extracellular signal-regulated kinase 1/2(ERK1/2) and nuclear factor kappa-B(NF-κB) after intracerebral hemorrhage. Blocking ERK1/2 activation, using the ERK1/2 inhibitor U0126(2 μg, intraventricular injection), reduced the level of NOX2-mediated oxidative stress induced by P2X7R activation after intracellular hemorrhage. Similarly, the inhibition of NF-κB, using the NF-κB inhibitor JSH-23(3.5 μg, intraventricular), reduced the level of NOX2-mediated oxidative stress induced by P2X7R activation. Finally, GSK2795039(100 mg/kg, intraperitoneal), a NOX2 antagonist, attenuated P2X7R-mediated oxidative stress, neurological damage, and brain edema after intracerebral hemorrhage. The results indicated that P2X7R activation aggravated NOX2-induced oxidative stress through the activation of the ERK1/2 and NF-κB pathways following intracerebral he

关键词： brain central nervous system factor inflammation injury pathways repair stroke

The Prediction of Rice Gene by Fgenesh

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Agricultural Sciences in China 2008年第4期7卷 387-394页

作者： ZHANG Sheng-li LI Dong-fang ZHANG gai-sheng WANG Jun-wei NIU na Shaanxi Key Laboratory of Crop Heterosis Northwest A ＆ F University Yangling 712100 P.R. China Key Discipline Open Laboratory on Crop Molecular Breeding Henan Institute of Higher Learning/Henan Institute of Science andTechnology Xinxiang 453003 P.R.China School of Resources and Environmental Sciences Henan Institute of Science and Technology Xinxiang 453003 P.R.China

This study has been carried out to give some scientific reasons for genome annotation, shorten the annotating time, and improve the results of gene prediction. Taking the sequence of the 6th chromosome, which has more length sequences than others, of Oryza sativa L. ssp. japonica cv. Nipponbare as analysis data in this research, the gene prediction of monocots module, rice, has been done by using Fgenesh ver. 2.0, and the predicting results have been explored particularly by bioinformatics methods. Results showed that the number of predicted genes for this chromosome was very close to the number of TIGR annotated genes. The majority of the predicted genes were multi-exon genes which had a percentage of 77.52. Length range was very big in the predicted genes. According to the significant match number, multi-exon genes can be predicted more veracity than single exon genes and the support can be reached up to 100% by TIGR annotation and up to 78% by cDna. From the angle of predicted exons location of multi-exon genes, the internal exons and last exons had a high support of cDna. The length of internal exons was relatively short in high （〉95% length, 〉78% similarity） cDna and/or TIGR annotation support multi-exon genes, but the first exons and last exons were on the reverse. The majority of single exon genes which had more than 95% in length, and 78% in similarity support by cDna and/or TIGR annotation was relatively short in length. From the angle of exon number, the majority of the multi-exon genes of high （〉 95% length, 〉 78% similarity） cDna and/or TIGR annotation support had no more than 5 exon number. It was concluded that the rice gene prediction by Fgenesh was very good but needed modification manually to some extent according to cDna support after aligning the predicting sequence of genes with cDna database of rice.

关键词： rice gene prediction cDna annotation exon

Apolipoprotein E Gene Polymorphisms Are Risk Factors for Spontaneous Intracerebral Hemorrhage:A Systematic Review and Meta-analysis

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Current Medical Science 2019年第1期39卷 111-117页

作者： Hao NIE Yang HU na LIU Ping ZHANG gai-gai LI Yan-yan LI Chao PAN Hai-han YU Qian WU Guo-feng WU Zhou-ping TANG Department of Neurology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Affiliated Hospital of Guizhou Medical University Guiyang 550004China

Intracerebral hemorrhage(ICH)is a serious clinical disease with high morbidity,whose pathogenesis might be related to apolipoprotein E(APOE)gene *** comprehensively evaluate the risk factors for ICH occurrence,we performed a *** searched online databases to identify eligible studies based on the relationship between APOE genetic polymorphisms and ICH occurrence *** and pooled odds ratios(ORs)were calculated and by assessing small study bias,we drew the relationship between APOE polymorphisms and ICH *** included 15 eligible studies in our study containing a total of 1642 ICH samples and 5545 normal *** comparison of e4 andε3 APOE genotypes revealed that specific and pooled ORs showed a significantly increased odds ratio in ICH patients with theε4 genotype,indicating thatε4 gene is a risk factor for ICH occurrence,and the heterogeneity is ***,it was found that theε2 genotype also contributed to the incidence rate of ***,after the subgroup analysis by ethnicity,this APOE genetic polymorphism acted as a harmful factor only in white populations,but did not show an effect in Asian *** was suggested that both 82 andε4 APOE alleles were risk factors for ICH in *** were risk factors in white populations only,neither had a detectable effect in Asian populations after subgroup analysing by ethnicity.

关键词： intracerebral hemorrhage apolipoprotein E allele genetic polymorphism metaanalysis

miR-137 inhibits melanoma cell proliferation through downregulation of GLO1

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Science China(Life Sciences) 2018年第5期61卷 541-549页

作者： na Lv Shuai Hao Chonglin Luo Alia Abukiwan Ying Hao Fei gai Weiwei Huang Lingyun Huang Xueyuan Xiao Stefan B.Eichmüller Dacheng He Key Laboratory of Cell Proliferation and Regulation of Ministry of Education Universities of the Confederated Institute for ProteomicsBeijing Normal University Beijing Engineering and Technology Research Center of Food Additives Beijing Technology and Business University Gene & Care Health Technologies Co. Ltd. GMP & T Cell Therapy Unit German Cancer Research Center (DKFZ) College of Sports Northwest Normal University

Late-stage melanoma is refractory to current therapies. MicroRnas (miRnas) can modulate many physiological and pathological processes of melanoma. Studies have demonstrated that miR-137 acts as a tumor suppressor by inhibiting the proliferation of melanoma cells through targeting multiple mRnas. The glyoxalase system member glyoxalase 1 (GLO1) is the principal scavenging enzyme of methylglyoxal (MG), a toxic byproduct of glycolysis. Using 35S in vivo/vitro labelling analysis for dynamic proteomics (SiLAD), we found that miR-137 downregulated the expression of GLO1 in melanoma *** analysis predicted that GLO1 is a direct target of miR-137. This was validated by dual luciferase reporter *** RT-PCR (qRT-PCR) and western blot analysis indicated that miR-137 could decrease endogenous GLO1 ***, siRna targeting of GLO1 mimicked inhibition of melanoma cell proliferation caused by miR-137 ***-expression of GLO1 was able to restore miR-137-mediated suppression of melanoma cell proliferation. Therefore, these results suggest that miR-137 inhibits the proliferation of melanoma cells by targeting GLO1.

关键词： melanoma miR-137 SiLAD GLO1 cell proliferation

Programmed cell death, antioxidant response and oxidative stress in wheat f lag leaves induced by chemical hybridization agent SQ-1

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Journal of Integrative Agriculture 2016年第1期15卷 76-86页

作者： WANG Shu-ping ZHANG gai-sheng SONG Qi-lu ZHANG Ying-xin LI Ying GUO Jia-lin CHEN Zheng NIU na MA Shou-cai WANG Jun-wei College of Agronomy Northwest A&F UniversityNational Yangling Agricultural Biotechnology & Breeding CenterYangling Branch of State Wheat Improvement CentreWheat Breeding Engineering Research Center Ministry of EducationKey Laboratory of Crop Heterosis of Shaanxi Province Institute of Genetics and Developmental Biology Chinese Academy of Sciences

Male sterility induced by a chemical hybridization agent （CHA） is an important tool for utilizing crop heterosis. Leaves, especially the flag leaves, as CHA initial recipients play a decisive role in inducing male sterility. To investigate effects of different treatment times of CHA-SQ-1 used, morphological, biochemical and physiological responses of wheat flag leaves were detected in thistudy. CHA induced programmed cell death （PCD） as shown in terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling （TUNEL） and Dna laddering analysis. In the early phase, CHA-SQ-1 trig- gered organelle changes arid PCD in wheat leaves accompanied by excess production of reactive oxygen species （O2- and H202） and down-regulation of the activities of superoxide dismutase （SOD）, catalase （CAT） and guaiacol peroxidase （POD）. Meanwhile, leaf cell Dnas showed ladder-like patterns on agarose gel, indicating that CHA-SQ-1 led to the activation of the responsible endonuclease. The oxidative stress assays showed that lipid peroxidation was strongly activated and photosynthesis was obviously inhibited in SQ-l-induced leaves. However, CHA contents in wheat leaves gradually reduced along with the time CHA-SQ-1 applied. Young flags returned to an oxidative/antioxidative balance and ultimately developed into mature green leaves. These results provide explanation of the relations between PCD and anther abortion and practical application of CHA for hybrid breeding.

关键词： wheat flag leaf chemical hybridization agent SQ-1 programmed cell death antioxidant response oxidative stress

Pre-sodiation strategy for superior sodium storage batteries

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Chinese Journal of Chemical Engineering 2021年第11期34卷 261-268页

作者： Yongkai Xu Haozheng Sun Cunshuang Ma Jingjing gai Yanhua Wan Weihua Chen Green Catalysis Center and College of ChemistryZhengzhou UniversityZhengzhou 450001China

The irreversible consumption of sodium in the initial several cycles greatly led to the attenuation of capacity,which caused the low initial coulombic efficiency(ICE)and obvious poor cycle *** can effectively improve the electrochemical performance by compensating the capacity loss in the initial ***,carbon-coated sodium-pretreated iron disulfide(naFeS_(2)@C)has been synthesized through conventional chemical method and used in sodium metal battery as a cathode *** calculated density of states(DOS)of naFeS2@C is higher,which implies enhanced electron mobility and improved cycle *** of the highly reversible conversion reaction and the compensation of irreversible capacity loss during the initial cycle,the na/naFeS_(2)@C battery achieves ultrahigh initial coulombic efficiency(96.7%)and remarkable capacity(751 mA·h·g^(-1) at 0.1 A·g^(-1)).In addition,highly reversible electrochemical reactions and ultra-thin naF-rich solid electrolyte interphase(SEI)also benefit for the electrochemical performance,even at high current density of 100 A·g^(-1),it still exhibits a reversible capacity of 136 mA·h·g^(-1),and 343 mA·h·g^(-1) after 2500 cycles at 5.0 A·g^(-1).This work aims to bring up new insights to improve the ICE and stability of sodium metal batteries.

关键词： Pre-sodiation Solid electrolyte interphase Initial coulombic efficiency Sodium metal batteries Interface Electrochemistry

Correlation between TGFβ1 Gene Polymorphism and Asthma in Baise, Guangxi Children

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Journal of Biosciences and Medicines 2024年第5期12卷 300-311页

作者： Fangxin Cao na Lin Juntao Lin gai Yang Xintong Wang Graduate School of Youjiang Medical University for nationalities Baise China Department of Pediatric Affiliated Hospital of Youjiang Medical University for Nationalities Baise China

Objective: This research was to study the correlation between the rs1800469, rs1800470, rs2241712, rs224171 and rs4803455 of TGFβ1 gene and asthma in Baise, Guangxi children. This research also studied the relationship between serum concentration of TGFβ1 and childhood asthma. Method: From June 2022 to December 2023, 121 children had physical examination in affiliated Hospital of Youjiang Medical University for nationalities were selected as control group and 118 children suffered from asthma in affiliated Hospital of Youjiang Medical University for nationalities during the same period were selected as asthma group. Result: There was no correlation between rs1800469, rs1800470, rs2241712, rs2241715, rs4803455 and asthma in Baise, Guangxi children. Linkage disequilibrium analysis showed that there were strong linkage disequilibrium among rs1800469, rs1800470, rs2241712, rs2241715 and rs4803455. Their haplotypes had no significant correlation with childhood asthma. The serum concentration of TGFβ1 in asthma group was lower than that in control group (p β1 had no significant relationship with the genotypes of rs1800469, rs1800470, rs2241712, rs2241715 and rs4803455.

关键词： TGFβ1 Chilhood Asthma SNP