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Molecular typing of methicillin and vancomycin-resistant Staphylococcus aureus isolated from clinical specimens by double locus sequence typing(DLST)method

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BIOCELL 2020年第3期44卷 411-419页

作者： SOHEILA HASANIAN nour amirmozafari AHMAD FARAJZADEH SHEIKH IRAJ MEHREGAN Department of Biology Science and Research BranchIslamic Azad UniversityTehranIran Department of Microbiology School of MedicineIran University of Medical SciencesTehranIran Department of Microbiology School of MedicineAhvaz Jundishapur University of Medical SciencesAhvazIran

Methicillin-resistant Staphylococcus aureus(MRSA)strains are the essential cause of infections in communities and *** present study was conducted to determine the molecular typing of MRSA,isolated from hospitalized patients,using the double-locus sequence typing(DLST).In total,280 *** isolated from clinical specimens by phenotypic(catalase,coagulase,DNase,oxacillin,vancomycin screening agar and antibiotic disk diffusion),and molecular methods(PCR for determining the mecA,vanA and nuc genes).The DLST and sequencing was performed for MRSA containing *** of 280 specimens,confirmed as Staphylococcus aureus(***),123(43.9%)strains were *** highest resistance toward the erythromycin(15μg),followed by ciprofloxacin(5μg),clindamycin(2μg),tetracycline(30μg),gentamicin(10μg)and rifampicin(5μg),was 98.3%,97.5%,94.3%,90.2%,83.7%and 41.4%,***,the least resistance(0%)was observed in each of teicoplanin(30μg),linzolide(30μg),and vancomycin(30μg).All(100%)of MRSA strains had the mecA,and none of them have had the *** results of DLST showed that the most common sequence types were BPH 2003 and *** DLST type 18-32 was a significant cluster of *** sequencing MRSA and comparing the dominant types via the DLST,it is possible to establish the etiology of the disease in a much shorter time,and prevent the complications of the ***,the combination of partial sequences of clfB and spa can serve as useful genetic markers for MRSA *** concluded that the MRSA in our region was relatively high,but no vancomycin resistance was *** majority of the MRSA DLST type was 18–32.

关键词： Antibiotic resistance DLST MRSA Staphylococcus aureus

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Biofilm formation in clinical isolates of nosocomial Acinetobacter baumannii and its relationship with multidrug resistance

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Asian Pacific Journal of Tropical Biomedicine 2016年第6期6卷 528-533页

作者： Ebrahim Babapour Azam Haddadi Reza Mirnejad Seyed-Abdolhamid Angaji nour amirmozafari Department of Microbiology Karaj BranchIslamic Azad University Medical Bacteriology Molecular Biology Research CenterBaqiyatallah University of Medical Sciences Department of Cell and Molecular Biology Faculty of Biological SciencesKharazmi University Iran University of Medical Sciences School of MedicineMicrobiology Department

Objective: To check biofilm formation by Acinetobacter baumannii(A. baumannii)clinical isolates and show their susceptibility to different antibiotics and investigate a possible link between establishment of biofilm and multidrug ***: This study was performed on clinical samples collected from patients with nosocomial infections in three hospitals of Tehran. Samples were initially screened by culture and biochemical tests for the presence of different species of Acinetobacter. Identifications were further confirmed by PCR assays. Their susceptibilities to 11 antibiotics of different classes were determined by disc diffusion method according to Clinical and Laboratory Standards Institute guidelines. The ability to produce biofilm was investigated using methods: culture on Congo red agar, microtiter plate, and test tube ***: From the overall clinical samples, 156 specimens were confirmed to contain A. baumannii. The bacteria were highly resistant to most antibiotics except polymyxin *** these isolates, 10.26% were able to produce biofilms as shown on Congo red ***, the percentage of bacteria with positive biofilm in test tube, standard microtiter plate, and modified microtiter plate assays were 48.72%, 66.66%, and 73.72%, respectively. At least 92% of the biofilm forming isolates were multidrug ***: Since most of the multidrug resistant strains produce biofilm, it seems necessary to provide continuous monitoring and determination of antibiotic susceptibility of clinical A. baumannii. This would help to select the most appropriate antibiotic for treatment.

关键词： Acinetobacter baumannii Biofilm Multidrug resistance Nosocomial infections

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Molecular cloning of virB12 gene of Brucella melitensis 16M strain in pET28a vector

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Asian Pacific Journal of Tropical Medicine 2012年第7期5卷 511-513页

作者： Shiva Mirkalantari nour amirmozafari Bahram Kazemi Gholamreza Irajian Tehran University of Medical Sciences School of MedicineMicrobiology DepartmentTehranIran Shahid Beheshli University of Medical Sciences Department of BiotechnologyTehranIran

Objective:To clone the virB12 gene in pET28a expression vector for production of recombinant protein to be used as antigenic component for future serological test ***: Brucella melitensis(***) 16M strain was cultured and bacterial DNA was extracted by Bioneer AccuPrep~ Genomic DNA Extraction *** primer pair was designed based on Brucella virB12 gene sequence with BamHI and HindIII restriction site at 5’ end of the forward and reverse primers,*** amplification was performed using PrimSTAR~ HS DNA polymerase and the PCK product was purified by DNA AccuPrepGel Purification *** DNA was cloned into pJET1.2 cloning ***12 gene fragment was excised from pJET1.2 asing BamHI/HindIII and subsequendy subcloned into pET28a(+).Results:Brucella virB12 gene was successfully cloned in pJET1.2 and then in pET28a(+) *** and restriction enzyme digestion confirms the ***:We cloned and expressed the Brucella virB12 gene which could be used as antigenic component for specific serological assay development.

关键词： Brucella Cloning virB12 pET28a pJE1.2

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Feature extraction for machine learning-based intrusion detection in IoT networks

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Digital Communications and Networks 2024年第1期10卷 205-216页

作者： Mohanad Sarhan Siamak Layeghy nour Moustafa Marcus Gallagher Marius Portmann University of Queensland BrisbaneQLD4072Australia University of New South Wales CanberraACT2612Australia

A large number of network security breaches in IoT networks have demonstrated the unreliability of current Network Intrusion Detection Systems(NIDSs).Consequently,network interruptions and loss of sensitive data have occurred,which led to an active research area for improving NIDS *** an analysis of related works,it was observed that most researchers aim to obtain better classification results by using a set of untried combinations of Feature Reduction(FR)and Machine Learning(ML)techniques on NIDS ***,these datasets are different in feature sets,attack types,and network ***,this paper aims to discover whether these techniques can be generalised across various *** ML models are utilised:a Deep Feed Forward(DFF),Convolutional Neural Network(CNN),Recurrent Neural Network(RNN),Decision Tree(DT),Logistic Regression(LR),and Naive Bayes(NB).The accuracy of three Feature Extraction(FE)algorithms is detected;Principal Component Analysis(PCA),Auto-encoder(AE),and Linear Discriminant Analysis(LDA),are evaluated using three benchmark datasets:UNSW-NB15,ToN-IoT and *** PCA and AE algorithms have been widely used,the determination of their optimal number of extracted dimensions has been *** results indicate that no clear FE method or ML model can achieve the best scores for all *** optimal number of extracted dimensions has been identified for each dataset,and LDA degrades the performance of the ML models on two *** variance is used to analyse the extracted dimensions of LDA and ***,this paper concludes that the choice of datasets significantly alters the performance of the applied *** believe that a universal(benchmark)feature set is needed to facilitate further advancement and progress of research in this field.

关键词： Feature extraction Machine learning Network intrusion detection system IoT

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Privacy-preserved learning from non-i.i.d data in fog-assisted IoT:A federated learning approach

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Digital Communications and Networks 2024年第2期10卷 404-415页

作者： Mohamed Abdel-Basset Hossam Hawash nour Moustafa Imran Razzak Mohamed Abd Elfattah Faculty of Computers and Informatics Zagazig UniversityZagazigSharqiyah44519Egypt School of Engineering and Information Technology University of New South Wales@ADFACanberraACT2600Australia Deakin University Geelong Waurn Ponds CampusAustralia Computer Science Department Misr Higher Institute for Commerce and ComputersMansoura35511Egypt

With the prevalence of the Internet of Things(IoT)systems,smart cities comprise complex networks,including sensors,actuators,appliances,and cyber *** complexity and heterogeneity of smart cities have become vulnerable to sophisticated cyber-attacks,especially privacy-related attacks such as inference and data poisoning *** Learning(FL)has been regarded as a hopeful method to enable distributed learning with privacypreserved intelligence in IoT *** though the significance of developing privacy-preserving FL has drawn as a great research interest,the current research only concentrates on FL with independent identically distributed(i.i.d)data and few studies have addressed the non-i.i.d *** is known to be vulnerable to Generative Adversarial Network(GAN)attacks,where an adversary can presume to act as a contributor participating in the training process to acquire the private data of other *** paper proposes an innovative Privacy Protection-based Federated Deep Learning(PP-FDL)framework,which accomplishes data protection against privacy-related GAN attacks,along with high classification rates from non-i.i.d ***-FDL is designed to enable fog nodes to cooperate to train the FDL model in a way that ensures contributors have no access to the data of each other,where class probabilities are protected utilizing a private identifier generated for each *** PP-FDL framework is evaluated for image classification using simple convolutional networks which are trained using MNIST and CIFAR-10 *** empirical results have revealed that PF-DFL can achieve data protection and the framework outperforms the other three state-of-the-art models with 3%–8%as accuracy improvements.

关键词： Privacy preservation Federated learning Deep learning Fog computing Smart cities

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A Study on the Second Order Tangent Bundles over Bi-K■hlerian Manifolds

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Chinese Annals of Mathematics,Series B 2024年第5期 777-804页

作者： nour Elhouda DJAA Aydin GEZER Abderrahim ZAGANE Relizane University Faculty of Sciences and Technology Department of Mathematics Ataturk University Faculty of Science Department of Mathematics

Abstract This paper aims to study the Berger type deformed Sasaki metric gBSon the second order tangent bundle T2M over a bi-K■hlerian manifold *** authors firstly find the Levi-Civita connection of the Berger type deformed Sasaki metric gBSand calculate all forms of Riemannian curvature tensors of this ***,they study geodesics on the second order tangent bundle T2M and bi-unit second order tangent bundle T21,1M,and characterize a geodesic of the bi-unit second order tangent bundle in terms of geodesic curvatures of its projection to the ***,they present some conditions for a sectionσ:M→T2M to be harmonic and study the harmonicity of the different canonical projections and inclusions of (T2M,gBS).Moreover,they search the harmonicity of the Berger type deformed Sasaki metric gBSand the Sasaki metric gSwith respect to each other.

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Surgeon Perspectives on Anastomotic Leaks and FluidAI’s StreamTM Platform: Integrating Qualitative Insights for Technological Innovation

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Surgical Science 2024年第8期15卷 492-500页

作者： Olivia Rennie Manaswi Sharma nour Helwa Department of Clinical Affairs FluidAI Medical (Formerly NERv Technology Inc.) Kitchener Canada Faculty of Medicine University of Toronto Toronto Canada

Purpose: This article investigates the critical importance of integrating surgeons’ direct input into the development of innovative technologies that address gaps in surgical care, including those aimed at reducing anastomotic leaks (AL), a major complication in gastrointestinal surgery. While traditional quantitative research methods are prevalent, they often overlook the invaluable insights of the surgeons who manage these complications firsthand. Subjects and Methods: This study employs a qualitative approach, utilizing semi-structured interviews with 40 surgeons from various specialties, including general, bariatric, colorectal, trauma, hepato-biliary, and thoracic surgery. The interviews were designed to probe the needs of surgeons, challenges currently faced, and gaps in clinical practice, research, and technology for detection and/or management of AL. The data were analyzed using thematic analysis, which revealed significant gaps in current technologies for early detection and prevention of leaks. Results: Surgeons expressed strong interest in FluidAI’s Stream™ Platform, a non-invasive medical device designed to monitor postoperative drainage fluid in real-time, providing continuous data on AL risk. The ability of this platform to offer early prediction through pH and electrical conductivity analysis was particularly appealing to participants, who emphasized the importance of timely interventions in improving patient outcomes. The study’s findings highlight not only the clinical challenges but also the emotional toll that AL takes on surgeons, underlining the need for innovations that are both data-driven and humanistic. Conclusion: By centering surgeons’ perspectives, this research advocates for a human-centered approach to technological advancement, ensuring that new tools are both clinically effective and aligned with the real-world needs of surgical practitioners.

关键词： Anastomotic Leaks Surgeon Perspectives Postoperative Complications Qualitative Inquiry Technological Innovation

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Chemically Mediated Interactions between Grapevine, Aphid, Ladybird, and Ant in the Context of Insect Chemical Ecology

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Phyton-International Journal of Experimental Botany 2024年第7期93卷 1523-1542页

作者： Taghreed Alsufyani noura J.Alotaibi nour Houda M’sakni Mona A.Almalki Eman M.Alghamdi Department of Chemistry College of ScienceTaif UniversityP.O.Box 11099Taif21944Saudi Arabia Department of Biology College of ScienceTaif UniversityP.O.Box 11099Taif21944Saudi Arabia Chemistry Department Faculty of ScienceKing AbdulAziz UniversityP.O.Box 80200Jeddah21589Saudi Arabia

This study simpliﬁes the complex relationship among grapevine plants,aphids,ladybirds,and ants,which is essential for effective pest management and ecological *** study investigated the impact of aphid attacks and the presence of ants and ladybirds on the volatile compounds proﬁle released into the chemosphere of the community consisting of the common vine Vitis vinifera,the aphid Aphis illinoisensis,the ladybird Cocci-nella undecimpunctata-and the ant Tapinoma *** study aims to analyze the volatile compounds emitted by the grapevine and surrounding insects in response to these intricate *** extraction of volatile organic compounds(VOCs)was carried out using closed-loop stripping(CLS)and then analyzed via gas chromatography-mass spectrometry(GC-MS)and principles coordinated analysis(PCA)was *** grapevine was exposed to different types and order of treatments,including non-infested,aphid-infested,aphid-infested with ant,aphid-infested with ladybird,and various combinations of ant and *** the aphid attack,the outcomes uncovered massive alterations in the volatile compound proﬁ*** grapevine displayed distinct emissions of germacrene D,an alcohol,and an alkene compared to non-infested *** characteristic VOC proﬁle was the share of infested grapes in the presence of ants,with benzene derivatives and sesquiterpenes dominating the *** coexistence of ladybirds with ants and aphids resulted in a dif-ferent volatile proﬁle characterized by elevated levels of aldehydes,ketones,α-farnesene,and its hydroxy *** was concluded that the emission of VOCs into the chemosphere of the grapevine communities varied qualitatively and quantitatively depending on the level of the relationship complexity within each community in response to the infestation of grapevines by aphids,the presence of ladybirds as natural predators,and the presence of ant as *** grapevine’s status-dependent compounds

关键词： Aphids Aphis illinoisensis volatile organic compounds chemosphere pest management gas chromatography-mass spectrometry closed-loop stripping

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An emerging terpolymeric nanoparticle pore former as an internal recrystallization inhibitor of celecoxib in controlled release amorphous solid dispersion beads:Experimental studies and molecular dynamics analysis

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Acta Pharmaceutica Sinica B 2024年第6期14卷 2669-2684页

作者： Jamie Anne Lugtu-Pe Xuning Zhang Sako Mirzaie Hao Han R.Chang nour AL-Mousawi Kuan Chen Yongqiang Li Anil Kane Daniel Bar-Shalom Xiao Yu Wu Advanced Pharmaceutics and Drug Delivery Laboratory Leslie Dan Faculty of PharmacyUniversity of TorontoToronto M5S 3M2Canada Candoo Pharmatech Company Inc. Mississauga L5N 5M1Canada Department of Pharmacy University of CopenhagenCopenhagen DK-2100Denmark Patheon by Thermo Fisher Scientific Toronto Region Operations(TRO)Mississauga L5N 3X4Canada

Solid oral controlled release formulations feature numerous clinical advantages for drug candidates with adequate solubility and dissolution ***,most new chemical entities exhibit poor water solubility,and hence are exempt from such *** combining drug amorphization with controlled release formulation is promising to elevate drug solubility,like other supersaturating systems,the problem of drug recrystallization has yet to be resolved,particularly within the dosage ***,we explored the potential of an emerging,non-leachable terpolymer nanoparticle(TPN)pore former as an internal recrystallization inhibitor within controlled release amorphous solid dispersion(CRASD)beads comprising a poorly soluble drug(celecoxib)reservoir and insoluble polymer(ethylcellulose)*** to conventional pore former,polyvinylpyrrolidone(PVP),TPN-containing membranes exhibited superior structural integrity,less crystal formation at the CRASD bead surface,and greater extent of celecoxib ***-atom molecular dynamics analyses revealed that in the presence of TPN,intra-molecular bonding,crystal formation tendency,diffusion coefficient,and molecular flexibility of celecoxib were reduced,while intermolecular H-bonding was increased as compared to *** work suggests that selection of a pore former that promotes prolonged molecular separation within a nanoporous controlled release membrane structure may serve as an effective strategy to enhance amorphicity preservation inside CRASD.

关键词： Controlled release amorphous solid dispersion Poorly soluble drug Internal recrystallization Membrane-reservoir coated beads Molecular dynamics simulation Effect of pore formers Terpolymer nanogel Drug-polymer interactions

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Dissolved Organic Matter Features of Three Adjacent Eastern Mediterranean Urbanized Watersheds

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Open Journal of Modern Hydrology 2024年第3期14卷 153-173页

作者： nour Abboud Elias Michel Maatouk Zeinab Matar Veronique Kazpard Research and Analysis Platform for Environmental Sciences (PRASE) Doctoral School of Sciences and Technology (EDST) The Lebanese University Rafic Hariri Campus Hadat Lebanon Laboratory of Georessources Geosciences and Environment (L2GE) Faculty of Sciences The Lebanese University Fanar Lebanon Department of Earth and Life Sciences Faculty of Sciences The Lebanese University Hadat Lebanon

Landscape urbanization broadly affects ecosystems in coastal watersheds, but, until now, the influence of nonpoint source urban inputs on dissolved organic matter (DOM) amount, composition, and source is poorly understood. To understand how DOM composition varied with urbanization, fluorescence excitation-emission matrices (EEMs) were determined for urban and non-urban waters from upstream to downstream sites along three adjacent coastal watersheds that flow into the Mediterranean Sea. Two humic DOM fluorescent components (humic-like and fulvic-like peaks) and two proteinic components (tyrosine-like and tryptophane-like peaks) were identified by EEM fluorescence. The results indicated that urbanization had an important influence on DOM concentration and composition, with urban waters having a high degree of DOM variation due to different land uses surrounding each body of water. Urban waters show a higher DOM fluorescence index (FI), the highest fluorescence intensity of protein-like manifested also by BIX values, and a lower value of the humification index (HIX) than non-urban waters which were dominated by allochthonous inputs. In addition, the EEM was compared in dry and wet season where higher DOM amounts and FI appeared in summer due to autochthonous production coming from algae growth compared to allochthonous input from rainfall dominated in wet season. The concentration of DOC increased from upstream to downstream for the three rivers, especially Beirut River. The increase in DOC values was observed in both dry and wet seasons by 39 and 19 times respectively compared to upstream (0.93 - 0.91 mgC/L).

关键词： Dissolved Organic Matter DOM EEM Fluorescence Spectroscopy Autochthonous/Allochthonous DOM Urbanization Upstream/Downstream Wet/Dry Season

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