Molecular typing of methicillin and vancomycin-resistant Staphylococcus aureus isolated from clinical specimens by double locus sequence typing(DLST)method

作     者：SOHEILA HASANIAN NOUR AMIRMOZAFARI AHMAD FARAJZADEH SHEIKH IRAJ MEHREGAN 

作者机构：Department of BiologyScience and Research BranchIslamic Azad UniversityTehranIran Department of MicrobiologySchool of MedicineIran University of Medical SciencesTehranIran Department of MicrobiologySchool of MedicineAhvaz Jundishapur University of Medical SciencesAhvazIran 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2020年第44卷第3期

页      面：411-419页

核心收录：

学科分类：100208[医学-临床检验诊断学] 1002[医学-临床医学] 10[医学] 

主　　题：Antibiotic resistance DLST MRSA Staphylococcus aureus 

摘      要：Methicillin-resistant Staphylococcus aureus(MRSA)strains are the essential cause of infections in communities and *** present study was conducted to determine the molecular typing of MRSA,isolated from hospitalized patients,using the double-locus sequence typing(DLST).In total,280 *** isolated from clinical specimens by phenotypic(catalase,coagulase,DNase,oxacillin,vancomycin screening agar and antibiotic disk diffusion),and molecular methods(PCR for determining the mecA,vanA and nuc genes).The DLST and sequencing was performed for MRSA containing *** of 280 specimens,confirmed as Staphylococcus aureus(***),123(43.9%)strains were *** highest resistance toward the erythromycin(15μg),followed by ciprofloxacin(5μg),clindamycin(2μg),tetracycline(30μg),gentamicin(10μg)and rifampicin(5μg),was 98.3%,97.5%,94.3%,90.2%,83.7%and 41.4%,***,the least resistance(0%)was observed in each of teicoplanin(30μg),linzolide(30μg),and vancomycin(30μg).All(100%)of MRSA strains had the mecA,and none of them have had the *** results of DLST showed that the most common sequence types were BPH 2003 and *** DLST type 18-32 was a significant cluster of *** sequencing MRSA and comparing the dominant types via the DLST,it is possible to establish the etiology of the disease in a much shorter time,and prevent the complications of the ***,the combination of partial sequences of clfB and spa can serve as useful genetic markers for MRSA *** concluded that the MRSA in our region was relatively high,but no vancomycin resistance was *** majority of the MRSA DLST type was 18–32.