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Intervention study on apoptosis of SH-SY5Y cells induced by manganese

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Intervention study on apoptosis of SH-SY5Y cells induced by ...

中国毒理学会第六届全国毒理学大会

作者： MA Jun-xiang CHEN Li YU Tong GAO Ai TIAN Lin niu pi-ye Department of Occupational Health and Environmental Health School ot Public HealthCapital Medical University Beijing Key Laboratory of Environmental Toxicology School of Public HealthCapital Medical University

OBJECTIVE To investigated the variation of apoptosis rate,necrosis rate and cell cycle in SH-SY5Y cells after they exposed to MnCI directly,or preconditioned with agonist(pyrroloquinoline quinine, PQQ) and suppressant(LY294002),respectively,and then exposed to MnCI2,which provided theoretical basis for the mechanism of Mn neurotoxicity and its possible *** The SH-SY5Y cells were cultured in Dulbecco’s modification of Eagle’s medium(Hyclone) supplemented with 10%fetal bovine serum(Gibco),penicillin 50 mg·L and streptomycin 100 mg·L at 37℃in a humidified air atmosphere containing 5%*** were divided into three groups,a non-pretreatment group and two intervention *** non-preconditioning group,SH-SY5Y cells were directly exposed to different concentrations of MnCI(0,0.5,1.0,2.0 and 4.0 mmol·L for 12 h,and the control was treated with 0.9%NaCI *** the two intervention groups,SH-SY5Y cells were preconditioned with 0.1μmol·L PQQ and 10μmol·L LY294002 for 1 h,respectively,and then treated with MnCI in the same way with non-preconditioning *** treatment,the cell viability,apoptosis rate,necrosis rate and every phases of cell cycle were detected by MTT and flow cytometry,*** The cell viability decreased in three groups compared to the control(P <0.01),and it dropped much more in LY294002 group than in non-preconditioning *** rate of cell apoptosis and necrosis showed obvious dose-response relationships,which significantly increased in three groups with concentrations of MnCI *** the increment in LY294002 group was much more than in non-preconditioning group,while the increment was less in PQQ group than in non-preconditioning *** ratio of G/G and G/M were significantly decreased but the percentage of S phase was obviously increased in three groups versus the control(P <0.01).And the decrements of G/G and G/M ratio and increment of the percentage of S phase in LY294002 group were much more than in non-

关键词： manganese apoptosis necrosis pyrroloquinoline quinine LY294002 SH-SY5Y cells

铝对大鼠脑组织脂质过氧化作用的影响及其性别差异

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中华劳动卫生职业病杂志 2006年第5期24卷 281-283页

作者：杨艳旭牛侨牛丕业娄静山西医科大学劳动卫生学教研室中国预防医学科学院

目的研究铝对大鼠脑组织脂质过氧化作用的影响及其性别差异,为进一步探讨铝的神经毒作用机制提供依据。方法将40只SD大鼠分为4组,每组10只,雌雄各半,每组大鼠分别腹腔注射0．0、2．0、4．0、8．0 AL／kg,连续3 d,间隔1 d,共60 d。用跳... 详细信息

目的研究铝对大鼠脑组织脂质过氧化作用的影响及其性别差异,为进一步探讨铝的神经毒作用机制提供依据。方法将40只SD大鼠分为4组,每组10只,雌雄各半,每组大鼠分别腹腔注射0．0、2．0、4．0、8．0 AL／kg,连续3 d,间隔1 d,共60 d。用跳台法测试其学习记忆能力的改变,用试剂盒测定其脑组织中的超氧化物岐化酶(SOD)活力和丙二醛(MDA)含量的改变,同时采用透射电镜观察其脑组织超微结构的变化。结果各组不同性别大鼠的各项指标的改变之间差异无统计学意义(P>0．05)。与对照组相比,染铝组大鼠的学习记忆能力明显降低,差异有统计学意义(P<0．05),脑组织内SOD活力降低、MDA含量增高,差异有统计学意义(P<0．05)。电镜观察可见染铝组大鼠大脑皮层神经细胞的膜结构破裂、溶解、消失。结论铝可能通过增强大鼠脑组织的脂质过氧化反应而产生神经毒作用,尚不能认为该作用有性别差异。

关键词：铝学习记忆脂质过氧化作用性别差异

Microarray analysis for IncRNA combined with mRNA expression profiles to identify hub IncRNA and mRNA involved in benzene hematotoxicity

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Microarray analysis for IncRNA combined with mRNA expression...

中国毒理学会第六届全国毒理学大会

作者： GAO Ai YANG Jing Bai Wen-lin CHEN Li ZHANG Le-feng MA Jun-xiang niu pi-ye TIAN Lin School of Public Health Capital Medical University

OBJECTIVE It was to identify key hub IncRNA and mRNA critical for benzene hematotoxicity. METHODS Microarray analysis for IncRNA combined with mRNA expression profiles was performed and the integrated analysis of IncRNA and mRNA expression was conducted to identify key hub genes,pathways and biological processes by using multiple bioinformatics *** 1) We identified 677 differentially expressed IncRNA and 1821 differentially expressed mRNA from four patients of chronic benzene poisoning,three benzene-exposed workers and three health controls matched age and gender without benzene exposure.2) Co-expression network of IncRNA-mRNA related to benzene hematotoxicity included 1984 connections among 89 IncRNAs and 726 *** main k-core subnets were identified and the majority of these genes were involved in immune response,hematopoiesis, B cell receptor signaling pathway and chronic myeloid ***017297,NR45623 and NR028291 might be closely associated with benzene *** LncRNA provided a rationale for the potential development of IncRNA-targeted approaches for the prevention and early diagnosis of benzene poisoning.

关键词： benzene IncRNA mRNA microarray analysis

Study on lysosomal injury induced by methyl tert-butyl ether in nerve cells

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Study on lysosomal injury induced by methyl tert-butyl ether...

中国毒理学会第九次全国毒理学大会

作者： MA Jun-xiang WANG Xiao-juan JI Hong-yun JIA Jia-xin ZHANG Shi-xuan CHEN Li niu pi-ye Department of Occupational Health and Environmental Health School of Public Health Capital Medical University Beijing Key Laboratory of Environmental Toxicology School of Public Health Capital Medical University

OBJECTIVE Lysosome morphology and function induced by methyl tert-butyl ether(MTBE) in HT-22 cells were investigated to elucidate the effect of MTBE toxicity. METHOD In this study, HT-22 cells were used. To obverse the toxicity of MTBE in HT-22 cells, we exposed cells to different concentrations of MTBE(0, 0.125, 0.25, 0.5, 1.0 mmol·L for 24 h, respectively, and the effect of MTBE on lysosome was investigated from lysosome morphology and function. The lysosome function was evaluated through detecting the expression of lysosomal enzyme CatB and catD and the expression of factor TFEB lysosome function. And the lysosome amount and the change of membrane permeability l were observed by a laser confocal microscope to indicate lysosome morphology. RESULTS(1) Effect of MTBE on lysosomal function: Compared with the control, MTBE could induce the decrease of Lysosomal cathepsin CatB and CatD expression and nuclear retention of TFEB, the core regulator of the lysosome, which suggested that MTBE can affect lysosome function of by reducing the expression of its important proteolytic enzymes cathepsin CatB and CatD and inhibiting its key regulator TFEB translocation from cytoplasm to nucleus.(2) Effect of MTBE on lysosomal morphology: After HT-22 cells treated with MTBE, the decrease in lysosome number and increase in the membrane permeability of lysosome membrane were observed under a laser confocal microscope. CONCLUSION MTBE can lead to the damage of lysosome from its structure and function.

关键词： Methyl tert-butyl ether(MTBE) Lysosome HT-22 cells

Heat shock protein 10 modulated apoptosis induced by manganese in SH-SY5Y cell lines

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Heat shock protein 10 modulated apoptosis induced by mangane...

中国毒理学会第六届全国毒理学大会

作者： CHEN Li SHI Zhi-xiong XIAO Zhong-xin GUO Cai-xia ZHANG Jie MA Jun-xiang GAO Ai TIAN Lin niu pi-ye Department of Occupational and Environmental Health School of Public HealthCapital Medical University Beijing Key Laboratory of Environmental Toxicology Capital Medical University

OBJECTIVE To examine cell viability,apoptosis and heat shock protein 10(Hsp10) mRNA expression changes caused by Mn in SH-SY5Y *** SH-SY5Y cells are divided into experimental group and control group:Group A(normal saline,0.9%NaCI) as control group, Group B(Mn 0.5 mmol·L),Group C(Mn 1.0 mmol·L),Group D(Mn 2.0 mmol·L), Group E(Mn 4.0 mmol·L) as experimental *** are exposed to Mn for 12 h,and then the cell viability was measured by MTT *** Apoptosis was detected using flow cytometry with An-nexin V-FITC/Propidium iodide(pi) ***,the mRNA expressions of Hsp10 in SH-SY5Y were measured by real -time *** After exposed to Mn,the cell viability of group B, C,D and E(94.50%,90.08%,83.95%and 79.40%respectively) are obviously decreased compared with group A(Ppi tag results,the rate of cell apoptosis in group B,C,D and E increased 1.27,9.23,5.38,and 3.19 times as high as normal saline control group respectively;compared with control group,the rate of cell apoptosis in group B increased significantly(P <0.05);the rate of cell apoptosis in group C,D and E increased clearly significantly(P<0.01).Real-time RT-PCR shows 0.95,0.84,0.63,0.46 folds increase of Hsp10 mRNA expression in group B,C,D,and E respectively compared with group *** SH-SY5Y cells,the rate of cell apoptosis increased but the Hsp10 mRNA decreased with the increased concentration of *** correlation analysis between the rate of cell apoptosis and Hsp10 mRNA showed a negative correlation(r=0.996,P<0.01),sugg

关键词： heat shock proteins manganese cells,cultureds

1,2-二氯乙烷对大鼠体外血脑屏障细胞损伤的形态学观察

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环境与职业医学 2005年第4期22卷 303-308页

作者：牛侨张勤丽王林平牛丕业山西医科大学山西太原030001

[目的]进一步探讨1,2-二氯乙烷对血脑屏障细胞的损伤作用。[方法]在体外建立了血脑屏障细胞模型, 并从形态学上观察1,2-二氯乙烷对血脑屏障细胞的损伤作用。体外培养脑微血管内皮细胞和神经胶质细胞,将生长良好的脑微血管内皮细胞和神... 详细信息

[目的]进一步探讨1,2-二氯乙烷对血脑屏障细胞的损伤作用。[方法]在体外建立了血脑屏障细胞模型, 并从形态学上观察1,2-二氯乙烷对血脑屏障细胞的损伤作用。体外培养脑微血管内皮细胞和神经胶质细胞,将生长良好的脑微血管内皮细胞和神经胶质细胞分为4组,即对照组、5、10、20μl/ml组,用1,2.二氯乙烷对脑微血管内皮细胞和神经胶质细胞染毒,染毒0、10、30、60 min后于光镜和电镜下密切观察细胞的形态学变化。[结果]染毒后的脑微血管内皮细胞呈进行性破坏,随着染毒剂量的加大细胞体积逐渐增大,胞膜模糊,细胞与细胞间、细胞与突触间连接减少, 甚至消失;随着染毒时间的延长,在10、30、60 min后可以观察到有进行性的细胞膜溶解破坏,胞核模糊,界限不清,突触变短。随着染毒浓度的增加或时间的延长,神经胶质细胞树突数量减少,轴突减少变短,细胞与突触间、胞体与胞体间连接减少甚至消失,超微结构显示胞浆中线粒体膜的破坏、缺失,嵴断裂、肿胀及空泡变,粗面内质网轻度扩张、脱颗粒,细胞核内染色质在核周凝集,胶质细胞间及突触间的连接松解。[结论]1,2-二氯乙烷可造成血脑屏障细胞的损伤,引起血管源性脑水肿。

关键词： 1,2-二氯乙烷血脑屏障脑微血管内皮细胞神经胶质细胞

Ultrastrong room-temperature phosphorescence in cucurbit[8]uril-mediated crystalline supramolecules for ratiometric detection of phenethylamine

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Science China Chemistry 2025年

作者： Qingyu niu Yingxiang ye Lingna Su Xu He Zhiqiang Li Yu Liu School of Chemical Engineering and Technology Hebei University of Technology Fujian Provincial Key Laboratory of Polymer Materials College of Chemistry and Materials Science Fujian Normal University Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education) Nankai University College of Chemistry State Key Laboratory of Elemento-Organic Chemistry Nankai University

It is a great challenge to modulate purely organic fluorescence-phosphorescence dual emission in aqueous solution because the excited singlet and triplet states are in competition with each other. Herein, we report a crystalline 1:2 host-guest formed by selfassembly of 4-(4-bromophenyl)-pyridinium derivatives and cucurbit[8]uril, in which a rigid environment rich in weak interactions is directly demonstrated. Due to the inhibition of non-radiative transition and stabilization of triplet exciton in the crystalline environment, the crystalline inclusion complex exhibits ultralong room-temperature phosphorescence lifetime of 3.21 ms with an room-temperature phosphorescence(RTP) quantum yield up to 16.29%. Interestingly, the crystalline 1:2 hostguest complex demonstrates the ability of rapid identification and sensitive fluorescence-phosphorescence ratiometric detection behavior toward phenethylamine, a drug analogue, with a limit of detection as low as 27.5 nM. We believe that this study will undoubtedly broaden the utilizing scopes of RTP and offer a novel avenue for psychoactive substances monitoring.

关键词：

高温、苯并芘-7,8-二醇-9,10-环氧化物单独作用对人肺腺癌细胞Hsp27、Hsp70表达的影响

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卫生研究 2006年第1期35卷 16-18页

作者：段燕英隆宗琰牛丕业邬堂春华中科技大学同济医学院公共卫生学院职业医学研究所和环境与健康教育部重点实验室武汉430030

目的研究不同温度和不同浓度B(a)P7,8二醇9,10环氧化物(BPDE)作用A549细胞后,热休克蛋白27(HSP2)、热休克蛋白70(Hsp70)的表达规律。方法体外培养的A549细胞分为高温应激组和BPDE组。37℃培养的细胞作对照,高温应激组细胞置于高温(39℃... 详细信息

目的研究不同温度和不同浓度B(a)P7,8二醇9,10环氧化物(BPDE)作用A549细胞后,热休克蛋白27(HSP2)、热休克蛋白70(Hsp70)的表达规律。方法体外培养的A549细胞分为高温应激组和BPDE组。37℃培养的细胞作对照,高温应激组细胞置于高温(39℃、42℃、43℃)应激2小时。BPDE组用不同浓度的BPDE(0、2、4、8μmolL)染毒2h。利用Westernblot技术分析A549细胞株中Hsp27、Hsp70的表达水平。结果高温应激组细胞Hsp27的表达较37℃对照明显升高,差异均有显著性(P<0.05)。在39℃,Hsp27的表达水平达到峰值。高温处理后,Hsp70的表达也增加,在42℃达到最高,且与对照相比差异有显著性(P<0.05)。BPDE组Hsp27、Hsp70的表达均较对照明显升高,其中Hsp27的表达水平在实验用最高浓度8μmolL达到最高,且与对照比较差异有显著性(P<0.05),但仍处于上升阶段。Hsp70在4μmolL达到最高,在4μmolL和8μmolL时,Hsp70的表达水平与对照比较差异有显著性(P<0.05)。结论高温和BPDE均能诱导A549细胞Hsp27、Hsp70的表达。高温应激时,Hsp27和Hsp70的表达水平分别在39℃和42℃达到峰值。BPDE处理时,Hsp27在8μmolL时表达最高,但仍处于上升趋势,而Hsp70在4μmolL时表达最高。

关键词： A549细胞 BPDE 高温热休克蛋白27 热休克蛋白70

“课程思政”融入环境卫生学教学的探索与实践

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环境卫生学杂志 2022年第5期12卷 396-398页

作者：李婕牛丕业首都医科大学公共卫生学院北京100069

环境卫生教学质量的提高对于公共卫生和预防医学人才的培养具有重要意义。本文结合课程特点及教学内容,从提升教师的思政素养、挖掘环境卫生学中的思政元素、环境卫生课程思政实践等方面探索“课程思政”融入环境卫生学的教学过程。

关键词：课程思政环境卫生学人才培养