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Pulmonary Immune Responses to Aspergillus Fumigatus in Rats

Biomedical and Environmental Sciences 2014年第9期27卷 684-694页

作者： MIRKOV Ivana EL-MUZGHI Amal Atia Mhfuod DJOKIC Jelena ninkov marina POPOV ALEKSANDROV Aleksandra GLAMOCLIJA Jasmina KATARANOVSKI Milena Department of Ecology Institute for Biological Research ‘Sinisa Stankovic'University of Belgrade Department of Plant Physiology (Mycology Lab) Institute for Biological Research ‘Sinisa Stankovic'University of Belgrade Institute of Physiology and Biochemistry Faculty of BiologyUniversity of Belgrade

Objective To evaluate immunologic mechanisms underlying Aspergillus fumigatus pulmonary infections in immunocompetent Dark Agouti （DA） and Albino Oxford （AO） rats recognized as being susceptible to some inflammatory diseases in different manners. Methods Lung fungal burden （quantitative colony forming units, CFU, assay）, leukocyte infiltration （histology, cell composition） and their function （phagocytosis, oxidative activity, CDllb adhesion molecule expression） and cytokine interferon-y （IFN-y） and interleukin-17 and -4 （IL-17 and IL-4） lung content were evaluated following infection （intratracheally, lxl07 conidia）. Results Slower reduction of fungal burden was observed in AO rats in comparison with that in DA rats, which was coincided with less intense histologically evident lung cell infiltration and leukocyte recovery as well as lower level of most of the their activities including intracellular myeloperoxidase activity, the capacity of nitroblue tetrazolium salt reduction and CDllb adhesion molecule expression （except for phagocytosis of conidia） in these rats. Differential patterns of changes in proinflammatory cytokine levels （unchanged levels of IFN-v and transient increase of IL-17 in AO rats vs continuous increase of both cytokines in DA rats） and unchanged levels of IL-4 were observed. Conclusion Genetically-based differences in the pattern of antifungal lung leukocyte activities and cytokine milieu, associated with differential efficiency of fungal elimination might be useful in the future use of rat models in studies of pulmonary aspergillosis.

关键词： A. fumigatus Rats Lung immune responses

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Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats

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Biomedical and Environmental Sciences 2019年第7期32卷 508-519页

作者： KULAS Jelena ninkov marina TUCOVIC Dina POPOV Aleksandrov Aleksandra UKROPINA Mirela CAKIC MILOSEVIC Maja MUTIC Jelena KATARANOVSKI Milena MIKROV Ivana Immunotoxicology group Department of Ecology Institute for Biological Research 'Sinisa Stankovic ' University of Belgrade 142 Bulevar despota Stefana Belgrade 11000 Serbia Institute of Zoology Faculty of Biology University of Belgrade 16 Studentskitrg Belgrade 11000 Serbia Innovation center of the Faculty of Chemistry University of Belgrade 12-16 Studentskitrg 11000 Belgrade Serbia Institute of Physiology and Biochemistry Faculty of Biology University of Belgrade 16 Studentskitrg Belgrade 11000 Serbia

Objective The aim of this study is to investigate the effects of oral cadmium(Cd) ingestion on the pulmonary immune response. Methods Determination of Cd content in lungs and histopathological evaluation of the tissue was performed in rats following 30-day oral Cd administration(5 and 50 mg/L). Antioxidant enzyme defense(superoxide dismutase and catalase), cell infiltration, and production of tumor necrosis factor(TNF) and interferon(IFN)-γ, as well as the activity of myeloperoxidase(MPO), nitric oxide(NO), and various cytokines [interleukin(IL)-1β, IL-6, IL-10, and IL-17] were investigated. Results Cd caused tissue damage and cell infiltration in the lungs, and this damage was more pronounced at higher doses. Cd deposition resulted in lung inflammation characterized by a dose-dependent IL-1β increase in lung homogenates, increased TNF levels at both doses, and IL-6 stimulation at low doses with inhibition observed at higher doses. Cd exerted differential effects on lung leukocytes isolated by enzyme digestion, and these effects were characterized by a lack of change in the production of reactive oxygen and nitrogen species, an inhibition of IL-1β and TNF, and stimulation of MPO and IFN-γ. The higher capacity of Cd-exposed lung cells to respond to the opportunistic pathogen Staphylococcus epidermidis was demonstrated in vitro. Conclusion The potential of ingested Cd to exert both proinflammatory and immunosuppressive effects on pulmonary tissue inflammation and immune reactivity highlights the complex immunomodulatory actions of this metal.

关键词： Rats Oral cadmium administration Lungs Cytokine response

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