Pulmonary Immune Responses to Aspergillus Fumigatus in Rats

作     者：MIRKOV Ivana EL-MUZGHI Amal Atia Mhfuod DJOKIC Jelena NINKOV Marina POPOV ALEKSANDROV Aleksandra GLAMOCLIJA Jasmina KATARANOVSKI Milena 

作者机构：Department of EcologyInstitute for Biological Research ‘Sinisa Stankovic'University of Belgrade Department of Plant Physiology (Mycology Lab)Institute for Biological Research ‘Sinisa Stankovic'University of Belgrade Institute of Physiology and BiochemistryFaculty of BiologyUniversity of Belgrade 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2014年第27卷第9期

页      面：684-694页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by the Ministry of Education Science and Technological Development of the Republic of Serbia Grant#173039 

主　　题：A. fumigatus Rats Lung immune responses 

摘      要：Objective To evaluate immunologic mechanisms underlying Aspergillus fumigatus pulmonary infections in immunocompetent Dark Agouti （DA） and Albino Oxford （AO） rats recognized as being susceptible to some inflammatory diseases in different manners. Methods Lung fungal burden （quantitative colony forming units, CFU, assay）, leukocyte infiltration （histology, cell composition） and their function （phagocytosis, oxidative activity, CDllb adhesion molecule expression） and cytokine interferon-y （IFN-y） and interleukin-17 and -4 （IL-17 and IL-4） lung content were evaluated following infection （intratracheally, lxl07 conidia）. Results Slower reduction of fungal burden was observed in AO rats in comparison with that in DA rats, which was coincided with less intense histologically evident lung cell infiltration and leukocyte recovery as well as lower level of most of the their activities including intracellular myeloperoxidase activity, the capacity of nitroblue tetrazolium salt reduction and CDllb adhesion molecule expression （except for phagocytosis of conidia） in these rats. Differential patterns of changes in proinflammatory cytokine levels （unchanged levels of IFN-v and transient increase of IL-17 in AO rats vs continuous increase of both cytokines in DA rats） and unchanged levels of IL-4 were observed. Conclusion Genetically-based differences in the pattern of antifungal lung leukocyte activities and cytokine milieu, associated with differential efficiency of fungal elimination might be useful in the future use of rat models in studies of pulmonary aspergillosis.