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胞外酸化经ASIC1/RIP1途径抑制TFEB介导的巨噬细胞脂噬

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生物化学与生物物理进展 2024年第1期51卷 202-214页

作者：刘娟欧翔刘情郭淼宁子萍顾洪丰唐雅玲南华大学基础医学院生理学教研室衡阳421001 南华大学附属长沙中心医院内分泌科长沙410004

目的探讨胞外酸化对巨噬细胞脂噬的影响及其作用机制。方法采用RAW264.7巨噬细胞,以pH 6.5培养液与25 mg/L氧化低密度脂蛋白(ox-LDL)共孵育24 h构建胞外酸化诱导的泡沫细胞模型。分别以ASIC1特异性阻断剂PcTx-1和RIP1抑制剂Nec-1干预... 详细信息

目的探讨胞外酸化对巨噬细胞脂噬的影响及其作用机制。方法采用RAW264.7巨噬细胞,以pH 6.5培养液与25 mg/L氧化低密度脂蛋白(ox-LDL)共孵育24 h构建胞外酸化诱导的泡沫细胞模型。分别以ASIC1特异性阻断剂PcTx-1和RIP1抑制剂Nec-1干预胞外酸化诱导的RAW264.7巨噬细胞24 h,油红O染色检测细胞内脂质蓄积;蛋白质印迹(Western blot)检测总ASIC1、膜ASIC1、p-RIP1 Ser166、p-TFEB Ser142、LC3和p62蛋白的表达;激光共聚焦显微镜观察脂滴(Bodipy示踪)与自噬标志物LC3II和LAMP1共定位;透射电镜观察细胞内脂滴和脂噬泡的数量变化;胆固醇荧光试剂盒检测ABCA1介导的胆固醇流出。结果与pH 7.4组相比较,pH 6.5胞外酸化组胞内的脂质蓄积和细胞质膜上的ASIC1蛋白表达显著增加,p-RIP1Ser166、p-TFEB Ser142水平升高,LC3II蛋白减少和p62蛋白增加,脂滴与LC3II和LAMP1的共定位都分别减少,细胞内的脂滴数量显著增加,自噬体和脂噬泡的数量则明显减少,ABCA1介导的巨噬细胞内胆固醇流出显著减少。然而,胞外酸化对RAW264.7巨噬细胞的上述效应能被ASIC1特异性阻断剂PcTx-1和RIP1抑制剂Nec-1所取消。结论胞外酸化经激活ASIC1/RIP1途径促进TFEB磷酸化抑制巨噬细胞脂噬,ASIC1可能是防治动脉粥样硬化等脂质蓄积疾病的新靶点。

关键词：酸敏感离子通道1 胞外酸化动脉粥样硬化脂噬受体相互作用蛋白1 转录因子EB

Advanced purification and comprehensive utilization of yellow phosphorous off gas

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Frontiers of Environmental Science & Engineering 2015年第2期9卷 181-189页

作者： ping ning Xiangyu WANG Faculty of Environmental Science and Engineering Kunrning University of Science and Technology Kunming 650500 China

Yellow phosphorous is an important raw material in the chemical industry. However, during the production of yellow phosphorous, high concentrations of carbon monoxide and other impurities are released. Without appropriate purification and removal, this off gas has potential to cause severe pollution problems once released. Purified yellow phosphorous off gas can be beneficially reused as a raw material in chemical production for synthesis of high value-added chemical reagents. In this paper, the significance of purification and reutilization of yellow phosphorous off gas are explored. The principles, processes, and main characteristics of the technologies for purification and reuse of yellow phosphorus off gas （including technical measurements of impurity reduction, relevant engineering cases, and public acceptance of the technologies） are summarized. In view of the existing problems and scientific development require- ments, this paper proposes green production based several recommendations for on the concept of recycle economics. We conclude that advanced purification and comprehensive reutilization can be an effective solution for heavy pollution resulting from yellow phosphorous off gassing.

关键词： yellow phosphorous off gas purification,comprehensive utilization

Novel synthesis of aluminum hydroxide gel-coated nano zero-valent iron and studies of its activity in flocculation-enhanced removal of tetracycline

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Journal of Environmental Sciences 2020年第3期32卷 194-205页

作者： Xiangyu Wang Binbin Zhang Jun Ma ping ning Faculty of Environmental Science and Engineering Kunming University of Science and TechnologyKunming 650500China School of Environment Harbin Institute of TechnologyHarbin 150090China

A newly designed aluminum hydroxide gel-coated nanoscale zero-valent iron(AHG@NZVI)with enhanced activity and dispersibility of NZVI was successfully *** AHG@NZVI composite was synthesized via control of the surface AHG ***@NZVI-1,AHG@NZVI-2 and AHG@NZVI-3 were prepared under centrifugal mixing speeds of 1000,2000 and 4000 r/min,*** activity of AHG@NZVI was evaluated by its tetracycline(TC) removal *** effects of AHG content,pH value,reaction temperature,and presence of competitive anions on TC removal were *** could be removed by both adsorption and chemical reduction on AHG@NZVI-2(centrifugal speed 2000 r/min) in a short time with high removal efficiency(≥98.1%) at the optimal *** excellent performance can be attributed to a synergistic interaction between aluminum hydroxide gel and NZVI:(1) AHG could enhance the stability and dispersity of NZVI;(2) aluminum hydroxide gel could absorb a certain amount of TC and Fe^2+/Fe^3+,which facilitated the mass transfer of TC onto the NZVI surface,resulting in acceleration of the reduction rate of TC by the AHG@NZVI composite;and(3) AHG-Fe^2+/Fe^3+could absorb a certain amount of TC by *** kinetics of TC removal by AHG@NZVI composite was found to follow a two-parameter pseudo-first-order *** presence of competitive anions slightly inhibited the activity of AHG@NZVI systems for TC ***,this study provides a promising alternative material and environmental pollution management option for antibiotic wastewater treatment.

关键词： Nanoscale zerovalent iron Aluminum hydroxide gel Synergistic effect Tetracycline Flocculation

选择性催化氧化H_(2)S的富氮碳催化剂研究进展

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中国环境科学 2024年第4期44卷 1981-1994页

作者：杨金涛杨学金宁平王访宋晓双贾丽娟冯嘉予云南民族大学化学与环境学院云南昆明650504 昆明理工大学环境科学与工程学院云南昆明650504

近年来,能高效净化H_(2)S并回收硫资源的选择性催化氧化技术(H_(2)S-SCO)受到了广泛关注.开发高性能、高选择性及低成本的催化剂是H_(2)S-SCO技术的研究重点,其中富氮碳基催化剂(RNCC)具有高活性、无金属、易制备、低成本且易再生等优点... 详细信息

近年来,能高效净化H_(2)S并回收硫资源的选择性催化氧化技术(H_(2)S-SCO)受到了广泛关注.开发高性能、高选择性及低成本的催化剂是H_(2)S-SCO技术的研究重点,其中富氮碳基催化剂(RNCC)具有高活性、无金属、易制备、低成本且易再生等优点,被认为是一类极具潜力的H_(2)S-SCO催化剂.本文介绍了金属基催化剂的发展,总结和归纳RNCC的制备方法、催化活性和物化性质,系统地讨论RNCC的构-效关系及影响RNCC性能的关键因素,并总结了RNCC的H_(2)S-SCO反应机理.最后指出RNCC目前存在机遇和挑战,并展望了未来的发展方向.

关键词：富氮碳基催化剂 H_(2)S 选择性催化氧化

铸造发动机用Mg-3.5Al-RE镁合金的组织与性能研究

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稀土 2024年第4期45卷 130-138页

作者：刘祥林波宁萍任飞迟成飞赵鹏展重庆科创职业学院重庆402160 重庆交通大学航运与船舶工程学院重庆400074 重庆大学机械与运载工程学院重庆400044 重庆城市职业学院重庆402160

采用真空熔炼+压铸的方法制备了Mg-3.5Al-RE镁合金(RE=La、Ce和Nd)和Mg-3.5Al-3Nd-0.9Zn-0.15Mn镁合金,研究了稀土元素和微量Zn、Mn元素对压铸镁合金物相组成、显微组织、力学性能、导热性能和流动性能的影响。结果表明,压铸Mg-3.5Al-R... 详细信息

采用真空熔炼+压铸的方法制备了Mg-3.5Al-RE镁合金(RE=La、Ce和Nd)和Mg-3.5Al-3Nd-0.9Zn-0.15Mn镁合金,研究了稀土元素和微量Zn、Mn元素对压铸镁合金物相组成、显微组织、力学性能、导热性能和流动性能的影响。结果表明,压铸Mg-3.5Al-RE镁合金主要由α-Mg基体、短棒状/鱼骨状Al_(11)RE_(3)或颗粒状Al_(2)RE相组成,平均晶粒尺寸从大至小顺序为:RE=La>RE=Ce>RE=Nd,抗拉强度、断后伸长率从高至低顺序为:RE=Nd>RE=Ce>RE=La,导热系数从高至低顺序为RE=Ce>RE=La>RE=Nd,RE=Nd时压铸镁合金具有最佳的综合拉伸性能。在Mg-3.5Al-RE镁合金中添加Zn和Mn元素后,镁合金中短棒状/鱼骨状Al_(11)Nd_(3)相明显较压铸Mg-3.5Al-3Nd镁合金少、颗粒状Al_(2)Nd相尺寸增大,室温拉伸性能与Mg-3.5Al-3Nd镁合金相当,显微硬度提高了7.02%,导热系数减小,流动长度增大。

关键词：压铸Mg-3.5Al-RE镁合金稀土元素显微组织流动性导热性力学性能

Interplay between Helicobacter pylori and immune cells in immune pathogenesis of gastric inflammation and mucosal pathology

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Cellular & Molecular Immunology 2010年第4期7卷 255-259页

作者： Hwei-Fang Tsai ping-ning Hsu Department of Internal Medicine Taipei Medical University Shuang Ho HospitalTaipeiTaiwan Gradute Institute of Clinical Medicine College of MedicineTaipei Medical UniversityTaipeiTaiwan Graduate Institute of Immunology College of MedicineNational Taiwan UniversityTaipeiTaiwan

Helicobacter pylori infection is associated with an inflammatory response in the gastric mucosa,leading to chronic gastritis,peptic ulcers,gastric carcinoma and gastric mucosa-associated lymphoid tissue(MALT)*** studies have shown that apoptosis of gastric epithelial cells is increased during *** *** induced by microbial infections are factors implicated in the pathogenesis of *** *** enhanced gastric epithelial cell apoptosis in *** infection has been suggested to play an important role in the pathogenesis of chronic gastritis and gastric *** addition to directly triggering apoptosis,*** induces sensitivity to tumor-necrosis factor(TNF)-related apoptosis-inducing ligand(TRAIL)-mediated apoptosis in gastric epithelial cells via modulation of TRAIL apoptosis ***,*** infection induces infiltration of T lymphocytes and triggers inflammation to augment *** *** infection,there was significantly increased CCR61CD31 T-cell infiltration in the gastric mucosa,and the CCR6 ligand,CCL20 chemokine,was selectively expressed in inflamed gastric *** results implicate that the interaction between CCL20 and CCR6 may play a role in recruiting T cells to the sites of inflammation in the gastric mucosa during Helicobacter *** these mechanisms,chemokine-mediated T lymphocyte trafficking into inflamed epithelium is initiated and the mucosal injury in Helicobacter infection is *** article will review the recent novel findings on the interactions of *** with diverse host epithelial signaling pathways and events involved in the initiation of gastric pathology,including gastric inflammation,mucosal damage and development of MALT lymphomas.

关键词： apoptosis CagA chemokine Helicobacter pylori TRAIL

Tumor necrosis factor: The key to hepatitis B viral clearance

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Cellular & Molecular Immunology 2018年第8期15卷 731-733页

作者： I-Tsu Chyuan ping-ning Hsu Department of Internal Medicine Cathay General HospitalTaipeiTaiwanChina Graduate Institute of Clinical Medicine College of MedicineNational Taiwan UniversityTaipeiTaiwanChina Graduate Institute of Immunology College of MedicineNational Taiwan UniversityTaipeiTaiwanChina Department of Internal Medicine National Taiwan University HospitalTaipeiTaiwanChina

The immunopathogenesis of hepatitis B virus(HBV)involves a complex interaction of innate and adaptive immune response associated with several ***,due to experimental limitations,very limited and even no activation of the innate immune response can be demonstrated in acute HBV *** role of innate immunity and the key innate effectors for HBV clearance is still *** is also not clear whether clinical use of TNF inhibitors in treating rheumatoid arthritis and other autoimmune disease patients with chronic HBV infection would affect viral ***,in a recent paper in Cell Mol Immunol,Chyuan et *** that under TNF-αblockade,HBV viral clearance was impaired with persistent elevated HBV viral load in a dose and temporal-dependent manner.1 It demonstrates that a deficiency of TNF-αreduces viral clearance and promotes HBV persistence in a mouse model,and the impairment of HBV clearance under anti-TNF-αoccurs at an early time point of HBV infection,suggesting that HBV may reactivate during therapy with TNF *** results together with previous reports support that TNF is a key innate cytokine required to clear HBV.2,3 It also provides evidence that therapy with TNF inhibitors may impair immune response to HBV clearance.

关键词： Immunol impaired immunity

Immobilization of NZVI in polydopamine surface-modified biochar for adsorption and degradation of tetracycline in aqueous solution

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Frontiers of Environmental Science & Engineering 2018年第4期12卷 87-97页

作者： XiangyuWang Weitao Lian Xin Sun Jun Ma ping ning Faculty of Environmental Science and Engineering Kunming University of Science and Technology Kunming 650500 China School of Municipal and Environmental Engineering State Key Laboratory of Urban Water Resources and Environment Harbin Institute of Technology Harbin 150090 China＊

Polydopamine/NZVl@biochar composite （PDA/NZVI@BC） with high removal efficiency of tetracycline （TC） in aqueous solutions was successfully synthesized, The resultant composite demonstrated high reactivity, excellent stability and reusability over the reaction course. Such excellent performance can be attributed to the presence of the huge surface area on biochar （BC）, which could enhance NZVI dispersion and prolong its longevity. The carbonyl group contained on the surface of bioehar could combine with the amino group on polydopamine（PDA）. The hydroxyl groups in PDA is able to enhance the dispersion and loading of NZVI on BC. Being modified by PDA, the hydrophilicity of biochar was improved. Among BC, pristine NZVI and PDA/NZVI（L-BC, PDA/ NZVI（a-,BC exhibited the highest activity for removal of TC. Compared with NZVI, the removal efficiency of TC could be increased by 55.9c- by using PDA/NZVI@BC under the same conditions. The optimal modification time of PDA was 813, and the ratio of NZVI to BC was 1：2. In addition, the possible degradation mechanism of TC was proposed, which was based on the analysis of degraded praducts by LC-MS. Difli,-rent importanl /actors impacling on TC removal （including mass ratio of NZVI to BC/PDA, initial concentration, pH value and the initial temperature of the solution） were investigated as well. Overall, this study provides a promising alternative material and environmental pollution management option for antibiotic wastewater treatment.

关键词： Biochar Polydopamine NZVI Modification Tetracycline

Reversibly enhanced aqueous solubilization of volatile organic compounds using a redox-reversible surfactant

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Journal of Environmental Sciences 2011年第9期23卷 1486-1490页

作者： Yingjie Li Senlin Tian Hong Mo ping ning Faculty of Environmental Science and Engineering Kunming University of Science and Technology Kunming 650093 China

Surfactant-enhanced remediation （SER） is an effective method for the removal of volatile organic compounds （VOCs） from contaminated soils and *** reuse the surfactant the VOCs must be separated from the surfactant *** water solubility of VOCs can be enhanced using reversible surfactants with a redox-acive group,（ferrocenylmethyl）dodecyldimethylammonium bromide （Fc12） and （ferrocenylmethyl）tetradecanedimethylammonium bromide （Fc14）,above and below their critical micelle concentrations （CMC） under reducing （I ＋） and oxidative （I 2＋） *** CMC values of Fc12 and Fc14 in I ＋ are 0.94 and 0.56 mmol/L and the solubilization of toluene by Fc12 and Fc14 in I ＋ for toluene is higher than the solubilization achieved with sodium dodecyl sulfate,cetyltrimethylammonium bromide and Trition *** solubilization capacity of the ferrocenyl surfactants for each tested VOCs ranked as follows： ethylbenzene toluene *** solubilities of VOCs by reversible surfactant in I ＋ were 30% higher than those in I 2＋ at comparable surfactant *** effects of Fc14 concentrations on VOCs removal efficiency were as follows： benzene toluene ***,an improved removal efficiency was achieved at low ferrocenyl surfactant ***,the reversible surfactant could be recycled through chemical approaches to remove organic pollutants,which could significantly reduce the operating costs of SER technology.

关键词： ferrocenyl surfactant reversibility solubility VOCs