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Molecularly Imprinted Polymer Based on GO-QDs as Enhanced Fluorescent Nanoscale Device for Specific Recognition of Target Protein

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Journal of Donghua University(English Edition) 2014年第5期31卷 603-608页

作者： DING Zhao-qiang GONG Xiao TAO Lei QUAN Jing nie hua-li ZHU li-min College of Chemistry Chemical Engineering and Biotechnology Donghua University Shanghai 201620 China National Engineering Research Center for Dyeing and Finishing of Textiles Donghua UniveLity Shanghai 201620 China

In this work the enhanced molecularly imprinted optosensing material based on graphene oxide-quantum dots （ GO- QDs） was synthesized for highly selective and sensitive specific recognition of the target protein, bovine serum albumin （BSA）. Here, GO was introduced to enhance the efficiency of mass-transfer in recognition of target protein. Molecularly imprinted polymer coated GO-QDs using BSA as template （BMIP-coated GO-QDs ） exhibited a fast mass-transfer speed, which could be ascribed to the high volume of efficient surface area and high target recognition efficiency of the synthesized nanoscale device. Under optimal conditions, it was found that the BSA as target protein could remarkably quench the relative fluorescence intensity of BMIP- coated GO-QDs linearly in a concentration-dependent manner that was best described by a Stern-Volmer equation. The Ksv （Stern- Volmer constant） for template BSA was much higher than bovine hemoglobin （BHb） and lysozyme （Lyz）, implying a highly selective recognition ability of the BMIP-coated GO-QDs to BSA. This enhanced fluorescent nanoscale device may provide opportunities to develop a system that is efficient and effective and has potential in the design of highly effective fluorescent receptor for recognition of target protein in Droteomics studies.

关键词： molecularly imprinted polymer (MIP) graphene oxide(GO) quantum dots (QDs) protein: fluorescent nanoscale device

Advancements in S-scheme heterojunction materials for photocatalytic environmental remediation

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Journal of Materials Science & Technology 2024年第2期169卷 182-198页

作者： Changliang nie Xiaohan Wang Ping Lu Yukun Zhu Xin li hua Tang School of Environmental Science and Engineering State Key Laboratory of Bio-fibers and Eco-textilesQingdao UniversityQingdao266071China Institute of Biomass Engineering Key Laboratory of Energy Plants Resource and UtilizationMinistry of Agriculture and Rural AffairsSouth China Agricultural UniversityGuangzhou510642China School of Environmental Science and Engineering Shandong UniversityQingdao266237China

Recently,S-scheme heterojunctions have gained considerable attention in the field of photocatalytic environmental remediation as their potential to achieve efficient spatial charge separation coupled with strong redox ***,this review provides an overview of the current state-of-the-art in the development of S-scheme-based photocatalysts for the purification of environmental *** review first covers the fundamentals of heterogeneous photocatalysis for environmental ***,an introduction to the background,mechanism,design principles,and characterization techniques of S-scheme heterojunctions is ***,the review presents a comparison and summary of using various S-scheme photocatalysts for the removal of several target pollutants,such as bacteria,heavy metals,nitrogen oxides,antibiotics,and ***,the modification strategies of S-scheme heterojunction photocatalysts are also ***,a brief discussion of the challenges and prospects associated with S-scheme photocatalytic systems is demonstrated.

关键词： S-scheme heterojunctions Photocatalyst Pollutant Environmental remediation

Determination of bioelectrochemical parameters of red cell using a piezoelectric crystal sensor

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Chinese Journal of Chemistry 1994年第5期12卷 432-439页

作者： SI, Shi-Hui nie, li-hua YAO, Shou-ZhuoNew material Research Institute, Hunan University, Changsha, Hunan 410082 China New material Research Institute Hunan University Changsha Hunan 410082 China

Based on the impedance behavior of red cell at high frequency, the frequency response of series piezoelectric crystal sensor in the red cell suspension was derived and verified experimentally. A method of using piezoelectric crystal sensor to determine the conductivity of the interior of the cell was proposed. The experimental results show that the mean conductivity of rabbit red cell cytoplasm was 0.269 S/m and the mean shape factor of red cell was 2.05.

关键词： Piezoelectric sensor conductivity cytoplasm.

Frequency characteristic of series piezoelectric sensor and application to the determination of water in organic solvents

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Chinese Journal of Chemistry 1994年第1期12卷 58-65页

作者： SHEN, Da-Zhong ZHU, Wen-Hong nie, li-hua YAO, Shou-Zhou New Material Research Institute, Hunan University, Changsha, Hunan 410082, China New Material Research Institute Hunan University Changsha Hunan 410082 China

Theoretical consideration was proposed for the frequency response of a series piezoelectric sensor to the properties of liquid and verified experimentally. This kind of sensor has sensitive and selective frequency response to the permittivity and specific conductivity of solution with excellent stability, and was applied to the determination of micro-content of water in organic solvents. The detection limit is 0.01 g/L in dioxane.

关键词： Piezoelectric quartz crystal chemical sensor micro-water-content

Piezoelectric transmission spectroelectrochemistry with a normal piezoelectric quartz crystal as an optically-transparent electrode

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Chinese Journal of Chemistry 1994年第5期12卷 425-431页

作者： XIE, Qing-Ji PANG, Xiao-Yong nie, li-hua YAO, Shou-Zhuo Department of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China Department of Chemistry and Chemical Engineering Hunan University Changsha Hunan 410082 China

Piezoelectric transmission spectroelectrochemistry (PTSEC), i.e., the combination of electrochemistry and spectroelectrochemistry (SEC) with electrochemical quartz crystal microbalance (EQCM) technique is reported by using a normal piezoelectric quartz crystal (PQC) as an optically-transparent electrode (OTE). A theoretical relationship between the PQC response and the spectroelectrochemical response is derived and used to estimate the apparent molar absorptivity of the absorbing species deposited on the OTE on the PQC surface. The complex of copper with aspartic acid is used to test this new PQC-SEC technique. Results show that the combination of three such diverse techniques provides a very useful methodology for studying electrode processes and electrode surface characteristics in situ.

关键词： Piezoelectronic transimission spectroelectrochemistry optically-transparent electrode electrochemical quartz crystal microbalance copper aspartic acid.

Piezoelectric　Fiber－optic　Spectroelectrochemistry──A　New　Technique　of　Spectroelectrochemistry　in　Combination　With　Piezoelectric　Quartz　Crystal　Detection

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Chemical Research in Chinese Universities 1994年第4期10卷 301-307页

作者： XIE Qing-ji HE Feng-jiao nie li-hua and YAO Shou-zhou(Department of Chernistry and Chelnical Engineering,Hunan University, Changsha, 410082)

iezoelectric fiber-optic spectroelectrochemistry(PFOSEC), a new techniqueof spectroelectrochemistry (SEC)in combination with piezoelectric quartz crystal(PQC)detection is proposed. In this technique, one arm of a bifurcated fiber-opticprobe is used to conduct the incident light beam to the investigated solution andthen to the reflective electrode surface on the piezoelectric quartz crystal, and thereflection light beam is guided to the optical detector by the other arm of the opticfiber. A typical experiinental system, Cu(Ⅱ)in glycine and KNO_3 supporting elec-trolyte, was used here to test this new technique. Results show that this techniquecan provide substantial in situ information from SEC, PQC and conventional elec-trochemistry in the investigation of the electrochemical process. This novel techiqueis recommended to find wider application in the electrochemical study not only onsolution species but also on surface phenomena.

关键词： Cornbined information SEC PQC Fiber-optic Copper Glycine

Optimal Design of the Modular Joint Drive Train for Enhancing Cobot Load Capacity and Dynamic Performance

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Chinese Journal of Mechanical Engineering 2024年第3期37卷 26-40页

作者： Peng li Zhenguo nie Zihao li Xinjun liu Department of Mechanical Engineering Tsinghua UniversityBeijing 100084China State Key Laboratory of Tribology in Advanced Equipment Tsinghua UniversityBeijing 100084China Beijing Key Lab of Precision/Ultra-precision Manufacturing Equipments and Control Tsinghua UniversityBeijing 100084China

Automation advancements prompts the extensive integration of collaborative robot(cobot)across a range of *** to the commonly used design approach of increasing the payload-to-weight ratio of cobot to enhance load capacity,equal attention should be paid to the dynamic response characteristics of cobot during the design process to make the cobot more *** this paper,a new method for designing the drive train parameters of cobot is ***,based on the analysis of factors influencing the load capacity and dynamic response characteristics,design criteria for both aspects are established for cobot with all optimization design criteria normalized within the design ***,with the cobot in the horizontal pose,the motor design scheme is discretized and it takes the joint motor diameter and gearbox speed ratio as optimization design ***,all the discrete values of the optimization objectives are obtained through the enumeration method and the Pareto front is used to select the optimal solution through multi-objective *** on the cobot design method proposed in this paper,a six-axis cobot is designed and compared with the commercial *** result shows that the load capacity of the designed cobot in this paper reaches 8.4 kg,surpassing the 5 kg load capacity commercial cobot which is used as a *** minimum resonance frequency of the joints is 42.70 Hz.

关键词： Multi-objective optimization Modular joint drive train design Load capacity Dynamic response performance

An　Oscillation　Condition　for　an　Electrode－separated　Piezoelectric　Sensor　in　Non－electrolyte　liquid

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Chemical Research in Chinese Universities 1995年第1期11卷 9-15页

作者： SHEN Da-zhong huaNG Ming-shan liN Song nie li-hua and YAO Shou-zhuo(New Material Research institute , Hunan University , Changsha , 410082) HUNAN UNIV NEW MAT RES INSTCHANGSHA 410082PEOPLES R CHINA

n oscillation condition was proposed and experimentally verified for an elec-troderaeparated piezoelectric sensor (ESPS) in non-electrolyte liquid, it was ex-pressed as , where C_s is the solution capaci-tance , R_q, C_o and w are the motional resistance , static capacitance and resonant an-gle frequency of the crystal , respectively. And Y-tgθ, where θ is the phase shift inthe oscillator. The relationships between the minimum cell constant needed for ES-PS to oscillate and each of the following parameters, permittivity, density and vis-cosity of the liquid as well as the oscillator phase shift were discussed. The″ cease-to-oscillate″ frequency of ESPS was measured. The oscillation ability of ESPS in-creases with increasing permittivity or decreasing density and viscosity of the liq-uid. An oscillator with a larger Y value is helpful to drive ESPS.

关键词： Piezoelectric sensor Quartz crystal Cell constant

Fabrication of micro-nano patterned materials mimicking the topological structure of extracellular matrix for biomedical applications

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Nano Research 2024年第5期17卷 4244-4258页

作者： Yifeng nie Dong Han Xiang li CAS Center for Excellence in Nanoscience National Center for Nanoscience and TechnologyBeijing 100190China School of Future Technology University of Chinese Academy of SciencesBeijing 100049China

With the advent of tissue engineering and biomedicine,the creation of extracellular matrix(ECM)biomaterials for in vitro applications has become a prominent and promising *** ECM materials provide physical,biochemical,and mechanical properties that guide cellular behaviors,such as proliferation,differentiation,migration,and *** micro-and nano-patterned materials have a unique surface topology and low energy replication process that directly affect cellular biological behaviors at the interface,the fabrication of micro-nano pattern biomaterials and the regulation of surface physical and chemical properties are of great significance in the fields of cell regulation,tissue engineering,and regenerative ***,we provide a comprehensive review of the progress in the fabrication and application of patterned materials based on the coupling of mechanical action at the micro-and nano-meter scale,including photolithography,micro-contact printing,electron beam lithography,electrospinning,and 3D printing ***,a summary of the fabrication process,underlying principles,as well as the advantages and disadvantages of various technologies are *** also discuss the influence of material properties on the fabrication of micro-and nano-patterns.

关键词： micro/nano hybrid materials patterning fabrication techniques extracellular matrix substrate-cell interaction biomedical engineering