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Secondary Structural Elements of the HCV X-region Involved in Viral Replication

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Journal of Clinical and Translational Hepatology 2015年第1期3卷 1-8页

作者： nidhi gupta Catherine H.Wu George Y.Wu Department of Medicine Division of Gastroenterology-Hepatology University of Connecticut Health Center Farmington CT USA

Background and Aims:The noncoding regions in the 3'-untranslated region (UTR) of the hepatitis C virus (HCV)genome contain secondary structures that are important for *** aim of this study was to identify detailed conformational elements of the X-region involved in HCV ***:Ribonucleic acid (RNA) structural analogs X94,X12,and X12c were constructed to have identical conformation but 94%,12%,and 0% sequence identity,respectively,to the X region of HCV genotype *** of structural analogs on replication of HCV genotypes 1b and 2a HCV RNA were studied by quantitative reverse transcriptase polymerase chain ***:In replicon BB7 cells,a constitutive replication model,HCV RNA levels decreased to 55%,52%,53%,and 54% after transfection with expression plasmids generating RNA structural analogs 5B-46,X-94,X-12,and X-12c,respectively (p<0.001 for all).In an HCV genotype 2a infection model,RNA analogs 5B-46,X-94,and X-12 in hepatic cells inhibited replication to 11%,9%,and 12%,*** the X-12 analog was only 12% identical to the corresponding sequence of HCV genotype 2a,the sequence per se,or antisense effects were unlikely to be ***:The data suggest that conformation of secondary structures in 3'-UTR of HCV RNA genome is required for HCV *** expression of RNA analogs predicted to have identical stem-loop structures might inhibit HCV infection of hepatocytes in liver and may represent a novel approach to design anti-HCV agents.

关键词： HCV RNA genome HCV X-region Hepatitis C virus Infection Japanese Fulminant Hepatitis Virus RNA secondary structure

Molecular Basis of Aluminium Toxicity in Plants: A Review

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American Journal of Plant Sciences 2013年第12期4卷 21-37页

作者： nidhi gupta Shailendra Singh Gaurav Ashwani Kumar Department of Biotechnology Chaudhary Charan Singh University Meerut India Department of Botany University of Rajasthan Jaipur India

Aluminium toxicity in acid soils having pH below 5.5, affects the production of staple food crops, vegetables and cash crops worldwide. About 50% of the world’s potentially arable lands are acidic. It is trivalent cationic form i.e. Al3+ that limits the plant’s growth. Absorbed Aluminium inhibits root elongation and adversely affects plant growth. Recently researches have been conducted to understand the mechanism of Aluminium toxicity and resistance which is important for stable food production in future. Aluminium resistance depends on the ability of the plant to tolerate Aluminium in symplast or to exclude it to soil. Physiological and molecular basis of Aluminium toxicity and resistance mechanism are important to understand for developing genetically engineered plants for Al toxicity resistance. This paper provides an overview of the state of art in this field.

关键词： Aluminium Toxicity Acid Soils Malate Citrate Wheat Field Crops

Amperometric Biosensor:Increased Sensitivity Using Enzyme Nanoparticles

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Amperometric Biosensor:Increased Sensitivity Using Enzyme Na...

The Molecular and Structural Basis of HBV-resistance to Nucleos(t)ide Analogs

International Conference on Nanotechnology and Biosensors（ICNB 2010）

作者： Shikha Sharma Aditi Shrivastav nidhi gupta Sudha Srivastava Dept.of Biotechnology Jaypee Institute of Information Technology

Here we present a comparative study of amperometric biosensor developed by using nanoparticles of Glucose oxidase enzyme itself （GOx np） with that of enzyme immobilized on to gold nanoparticles （Au np-GOx）.The biosensor developed by immobilizing the GOx np （45-70 nm in size） directly onto the *** could detect glucose concentrations as low as 1 mM while the one with Au np-GOx （Au np 10-16 nm in size） showed a detection limit up to 5 mM *** response showed linearity up to 35 mM in both the *** the best of our knowledge the glucose biosensors prepared using various metal nanoparticles,Au,Ni,Ag （as reported in literature） showed a linear range from 2.7-30 mM glucose *** are in the process of optimizing the immobilization methods and functionalization of the enzyme nanoparticles so as to have a wider temperature and pH stability of the *** feel the direct immobilization of enzyme nanoparticles,with proper choice of functionalization seems to be a promising strategy for biosensor development with higher sensitivities.

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Journal of Clinical and Translational Hepatology 2014年第3期2卷 202-211页

作者： nidhi gupta Milky Goyal Catherine H.Wu George Y.WU Department of Medicine Division of Gastroenterology-HepatologyUniversity of Connecticut Heath CenterFarmingtonUSA Department of Microbiology College of Basic Sciences and HumanitiesPunjab Agriculture UniversityLudhianaPunjabIndia

Infection with hepatitis B virus(HBV)is a worldwide health *** hepatitis B can lead to fibrosis,liver cirrhosis,and hepatocellular carcinoma(HCC).Management of the latter two conditions often requires liver *** with conventional interferon or pegylated interferon alpha can clear the virus,but the rates are very *** likelihood,however,of viral resistance to interferon is *** main problems with this therapy are the frequency and severity of side *** contrast,nucleos(t)ide analogs(NAs)have significantly lower side effects,but require long term treatment as sustained virological response rates are extremely ***,long term treatment with NAs increases the risk for the development of anti-viral drug *** by understanding the molecular basis of resistance and using agents with multiple sites of action can drugs be designed to optimally prevent the occurrence of HBV antiviral resistance.

关键词： HBV HCC Nucleos(t)ide analogs

旋转电机定子线圈/线棒的冲击耐电压试验要求及提高绝缘系统可靠性的创新措施

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国外大电机 2022年第2期 30-34页

作者： [印度]nidhi gupta 魏松(译) 陈阳(校) 不详

0引言高电压定子绕组的绝缘系统由绝缘和半导体材料组成,这些材料应用于合适的位置以实现特定的功能。这些材料具有某些特定的特性,使其适合在合适的位置使用。

关键词：绝缘系统定子绕组耐电压试验线棒创新措施半导体材料高电压系统可靠性

Intraoperative magnetic resonance imaging in neurosurgery and anesthetic considerations

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World Journal of Anesthesiology 2014年第2期3卷 174-180页

作者： nidhi gupta Girija Prasad Rath Unit of Neuroanaesthesia Fortis Memorial Research Institute Department of Neuroanaesthesiology Neurosciences Centre

Currently, magnetic resonance imaging(MRI) is the only imaging modality available which is capable of acquiring intra-operative images frequently with acceptable spatial and contrast resolution. However, the incorporation of MRI technology into the operating room requires special anesthetic considerations. It may include various aspects such as transport, remote location anesthesia, strong electromagnetic field, use of approved items, equipment counts, possible emergencies, and surgery in awake patients. The patient safety may be compromised by health-related, equipment-related, and procedure-related risks. Direct patient observation may be compromised by acoustic noise, darkened environment, obstructed line of sight, and distractions along with difficult access to the patient for airway management. Most often, the patient's head will be 180° away from the anesthesiologist during the procedure. Several monitors exist that are designed for conditional use in a MR environment. The general design criterion in these monitors is to eliminate conductors that carry electrical signals for monitoring physiologic parameters of the patient. General anesthesia requires an extended anesthetic circuit for ventilation maintenance and drug administration because the patient is located farther from the anesthesia machine than in traditional operating room settings. Dead space creates a time delay before the volatile anesthetic and drugs are administered and when expected effects can be observed. Therefore, the attending anaesthesiologists must understand the above aspects for safe conduct of neurosurgical procedures by minimizing MRI associated accidents while assuring optimal patient vigilance.

关键词： Intraoperative magnetic resonance imaging Electromagnetic field Safety Anesthesia Neurosurgery

Disease detection,severity prediction,and crop loss estimation in MaizeCrop using deep learning

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Artificial Intelligence in Agriculture 2022年第1期6卷 276-291页

作者： nidhi Kundu Geeta Rani Vijaypal Singh Dhaka Kalpit gupta Siddaiah Chandra Nayaka Eugenio Vocaturo Ester Zumpano Department of Computer and Communication Engineering Manipal University JaipurJaipurIndia ICAR DOS in Biotechnology University of Mysore ManasagangotriMysore 570005India DIMES(Department of Computer Engineering ModelingElectronics and Systems)University of CalabriaItaly Nanotec Italian National Research Council87036 RendeCSItaly

The increasing gap between the demand and productivity of maize crop is a point of concern for the food industry,and ***'susceptibility to diseases such as Turcicum Leaf Blight,and Rust is a major cause for reducing its *** detection,and classification of these diseases,calculation of disease severity,and crop loss estimation is a time-consuming ***,it requires expertise in disease ***,there is a need to find an alternative for automatic disease detection,severity prediction,and crop loss *** promising results of machine learning,and deep learning algorithms in pattern recognition,object detection,and data analysis motivate researchers to employ these techniques for disease detection,classification,and crop loss estimation in maize *** research works available in literature,have proven their potential in automatic disease detection using machine learning,and deep learning ***,there is a lack none of these works a reliable and real-life labelled dataset for training these ***,none of the existing works focus on severity prediction,and crop loss *** authors in this manuscript collect the real-life dataset labelled by plant *** propose a deep learning-based framework for pre-processing of dataset,automatic disease detection,severity prediction,and crop loss *** uses the K-Means clustering algorithm for extracting the region of ***,they employ the customized deep learning model‘MaizeNet’for disease detection,severity prediction,and crop loss *** model reports the highest accuracy of 98.50%.Also,the authors perform the feature visualization using the ***,the proposed model is integrated with a web application to provide a userfriendly *** efficacy of the model in extracting the relevant features,a smaller number of parameters,low training time,high accuracy favors its importance as an assisting tool for plant pathology experts.T

关键词： Disease detection Crop loss Severity Deep learning Maize

Local infiltration analgesia following total hip replacement： a review of current literature

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Chinese Journal of Traumatology 2014年第5期17卷 293-297页

作者： Tushar gupta nidhi Garg Mayank gupta Department of Orthopaedics All India Institute of Medical Sciences New Delhi India Department of Anaesthesiology All India Institute of Medical Sciences New Delhi India Department of Surgery Dayanand Medical College Ludhiana India

在全部的新潮的代替以后控制手术后的疼痛是重要的，争吵关于不同政体留下。由考察 18 研究，我们试图发现在全部的新潮的代替以后的本地渗入痛觉缺失是否在手术后的疼痛，一些医院停留和 opioid 消费上有任何效果。我们断定本地渗入痛... 详细信息

在全部的新潮的代替以后控制手术后的疼痛是重要的，争吵关于不同政体留下。由考察 18 研究，我们试图发现在全部的新潮的代替以后的本地渗入痛觉缺失是否在手术后的疼痛，一些医院停留和 opioid 消费上有任何效果。我们断定本地渗入痛觉缺失是为减少的手术后的疼痛的一个有效方法。

关键词：髋关节置换浸润麻醉文献药物用量疼痛

Outbreak of Salmonella Typhi enteric fever in sub-urban area of North India:A public health perspective

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Asian Pacific Journal of Tropical Medicine 2013年第2期6卷 167-168页

作者： nidhi Singla Neha Bansal Varsha gupta Jagdish Chander Department of Microbiology Government Medical College HospitalChandigarh160030India

Outbreaks of enteric fever are a major health concern not only due to significant human morbidity and mortality but also fear of spread of multidrug resistant *** report an outbreak of enteric fever caused by Salmonella enterica serotype Typhi in a suburban area,in city Chandigarh of North ***-seven strains of *** were isolated from blood cultures over a period of two weeks with 18 of these 27 patients residing in the same *** cases were in the age group 5-14 years(10 patients,55.5%) while 4(22.2%) cases were children under 5 *** the strains showed similar resistogram being resistant to ampicillin and nalidixic acid,intermediate to ciprofloxacin and sensitive to chloramphenicol,ceftriaxone,cefotaxime,cotrimoxazole and azithromycin on disc diffusion *** inhibitory concentration of ciprofloxacin was determined by agar dilution method and was found to be raised(≥2μg/mL).This nalidixic acid resistant *** outbreak report warrants the necessity of implementing stringent sanitation practices in public health interest.

关键词： Enteric fever Fluoroquinolones Outbreak Salmonella