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Effect of Freeze-Thaw and Urea in Solubility of GPC3-Csub Protein Expressed in Escherichia coli

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Journal of Biosciences and Medicines 2024年第4期12卷 288-297页

作者： Xuan-Truc Chu-Dao Kim-Tuyen Huynh-Dam Dang-Thuc Ngo-Luong Quang-Luan Le Thanh-thao Vo-nguyen Department of Medical Biotechnology Biotechnology Center of Ho Chi Minh City Ho Chi Minh City Vietnam

Glypican-3 is a protein encoded by the Glypican-3 gene located on human X chromosome (Xq26), composed of two subunits, a 40 kDa N-terminal subunit, and a 30 kDa C-terminal subunit. Glypican-3 is a currently potential target molecule for liver cancer treatments because of its over-expression and growth effects on hepatocellular carcinoma (HCC). this study examined the expression and purification of a C-terminal subunit of Glypican-3 protein (GPC3-Csub) due to its application in both diagnosis and therapy for hepatocellular carcinoma. The gene encoding for GPC3-Csub was successfully cloned into plasmid pET28a fused with an affinity tag composed of six consecutive histidine residues (His-tag). Recombinant protein GPC3-Csub was expressed in Escherichia coli BL21 (DE3) in the condition of adding 3% ethanol with IPTG induction. GPC3-Csub was extracted using repeated freeze-thaw cycles with lysozyme, and inclusion bodies were solubilized by 8M Urea, SDS 10% in pH 12. His-tag fused GPC3-Csub proteins allowed it to be purified by affinity chromatography method using the Nickel-nitrilotriacetic acid (Ni-NTA) column. High expression of GPC3-Csub was confirmed by Coomassie staining and western-blot. GPC3-Csub could be isolated with a Ni-NTA column and have a purity of about 90%.

关键词： Glypican-3 Affinity Chromatography Inclusion Body Liver Cancer

Evaluation of five gridded rainfall datasets in simulating streamflow in the upper Dong Nai river basin,Vietnam

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International Journal of Digital Earth 2019年第3期12卷 311-327页

作者： Pham thi thao Nhi Dao nguyen Khoi nguyen Xuan Hoan Institute of Research and Development Duy Tan UniversityDa NangVietnam Institute for Computational Science and Technology Tan Hiep Chanh WardDistrict 12Ho Chi Minh CityVietnam Faculty of Environment University of ScienceVietnam National University Ho Chi Minh City227 Nguyen Van Cu StreetDistrict 5Ho Chi Minh CityVietnam Ho Chi Minh City University of Food Industry 140 Le Trong Tan StreetTan Phu DistrictHo Chi Minh CityVietnam

Rainfall data with an appropriate spatial resolution is a key input to hydrological ***,networks of rain gauges are often sparsely and unevenly distributed in large catchments,especially in developing ***-resolution rainfall datasets,such as the Asian Precipitation Highly Resolved Observational Data Integration Towards Evaluation of Water Resources(APHRODITE),the Climate Forecast System Reanalysis(CFSR),the Climatic Research Unit Time Series(CRUTS),the Global Precipitation Climatology Centre(GPCC)and the Tropical Rainfall Measuring Mission(TRMM),have become available to overcome such *** objective of this study was to evaluate the impacts of four land-based rainfall products(APHRODITE,CFSR,CRU-TS,and GPCC)and a satellite-based rainfall product(TRMM)on streamflow of the upper catchment of Tri An reservoir in Vietnam using the Hydrological Modeling System(HEC-HMS).In addition,the available rain gauges data were used for comparison *** indicates that the TRMM and GPCC data show their best match to rain gauges data in simulating the streamflow in the period 1999–***,the results indicate that the TRMM and GPCC data could be alternative solutions.

关键词： APHRODITE CFSR CRU-TS GPCC TRMM rainfall streamflow HEC-HMS model

Orange G degradation by heterogeneous peroxymonosulfate activation based on magnetic MnFe_(2)O_(4)/α-MnO_(2) hybrid

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Journal of Environmental Sciences 2023年第2期124卷 379-396页

作者： Le thi thao To Van nguyen Van Quy nguyen Ngoc Man Phan Ki Jae Kim nguyen Nhat Huy nguyen Trung Dung Faculty of Physical and Chemical Engineering Le Quy Don Technical UniversityHanoi 100000Vietnam Department of Energy Engineering Konkuk UniversitySeoul 05029Korea School of Chemical Engineering College of EngineeringSungkyunkwan UniversityJangan-guSuwon 16419Korea Faculty of Environment and Natural Resources Ho Chi Minh City University of Technology(HCMUT)Ho Chi Minh City 700000Vietnam Vietnam National University Ho Chi Minh City Linh Trung WardThu Duc CityHo Chi Minh City 700000Vietnam

Wastewater containing an azo dye Orange G(OG)causes massive environmental pollution,thus it is critical to develop a highly effective,environmental-friendly,and reusable catalyst in peroxymonosulfate(PMS)activation for OG *** this work,we successfully applied a magnetic MnFe_(2)O_(4)/α-MnO_(2) hybrid fabricated by a simple hydrothermal method for OG removal in *** characteristics of the hybrid were investigated by Xray diffraction,scanning electron microscopy,energy-dispersive X-ray spectroscopy,Fourier transform infrared spectroscopy,Brunauer–Emmett–Teller method,vibrating sample magnetometry,electron paramagnetic resonance,thermogravimetric analysis,and X-ray photoelectron *** effects of operational parameters(i.e.,catalytic system,catalytic dose,solution pH,and temperature)were *** results exhibited that 96.8% of OG degradation was obtained with MnFe_(2)O_(4)/α-MnO2(1:9)/PMS system in 30 min regardless of solution pH ***,the possible reaction mechanism of the coupling system was proposed,and the degradation intermediates of OG were identified by mass *** radical quenching experiments and EPR tests demonstrated that SO_(4)•̶,O_(2)•̶,and 1O2 were the primary reactive oxygen species responsible for the OG *** hybrid also displayed unusual stability with less than 30%loss in the OG removal after four sequential ***,magnetic MnFe2O4/α-MnO2 hybrid could be used as a high potential activator of PMS to remove orange G and maybe other dyes from wastewater.

关键词： MnFe_(2)O_(4)/α-MnO_(2) Peroxymonosulfate Orange G Catalytic degradation Reusability

Polygonatum kingianum rhizome extract alleviates collagen antibody-induced arthritis by modulating proinflammatory cytokine production in mice

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Asian Pacific Journal of Tropical Biomedicine 2020年第11期10卷 490-495页

作者： Huyen Do thi Thanh Phuong Ngo thi Hien nguyen thi Thu Nga nguyen thi Hung Le Ngoc thao Dothi Ha Le Minh Graduate University of Science and Technology Vietnam Academy of Science and Technology18 Hoang Quoc VietNghia DoCau GiayHanoiVietnam Institute of Natural Products Chemistry Vietnam Academy of Science and Technology18 Hoang Quoc VietNghia DoCau GiayHanoiVietnam Hanoi Open University101 nguyen Hien str Hai Ba Trung Dist.HanoiVietnam Laboratory of Bioassay Institute of BiotechnologyVietnam Academy of Science and Technology18 Hoang Quoc VietNghia DoCau GiayHanoiVietnam Chemistry Lab Project Center for Research and Technology transferVietnam Academy of Science and Technology18 Hoang Quoc VietNghia DoCau GiayHanoiVietnam

Objective: To evaluate the anti-arthritic effects of Polygonatum kingianum rhizome extract using both in vitro and in vivo ***: Lipopolysaccharide-induced RAW 264.7 macrophages were treated with an ethanol extract of Polygonatum kingianum rhizomes at different concentrations to determine nitric oxide and prostaglandin E2(PGE2) production. For in vivo study, Polygonatum kingianum ethanol extract was further investigated for its antiinflammatory effect in a mouse model with collagen antibodyinduced arthritis. Phytochemical study of Polygonatum kingianum ethanol extract was also ***: Saponins(142 mg/g total yield) was the main component in the Polygonatum kingianum ethanol extract. 5α,8α-ergosterol peroxide,(E,E)-9-oxooctadeca-10,12-dienoic acid and 3-(2?-hydroxy-4?-methoxy-benzyl)-5,7-dihydroxy-8-methyl-chroman-4-one were isolated from the extract. Polygonatum kingianum ethanol extract exhibited potential anti-inflammatory effects by inhibiting nitric oxide and PGE2 production in RAW 264.7 cells in a dosedependent manner. The level of arthritis in mice with collagen antibody-induced arthritis was significantly reduced(P<0.01) after treatment with Polygonatum kingianum ethanol extract, particularly at a dose of 1 000 mg/kg body weight. Besides, the extract demonstrated the regulatory effects on serum tumor necrosis factoralpha, interleukin-6, and interleukin-10 in treated ***: Polygonatum kingianum ethanol extract has beneficial effects on inflammatory cytokine regulation and PGE2 inhibition in an experimental mouse model with collagen antibody-induced arthritis. The phytochemical screening reveals that the saponin, as the main component, and sterols(daucosterol and 5α,8α-ergosterol peroxide) from Polygonatum kingianum ethanol extract may contribute to its promising in vitro and in vivo anti-inflammatory activities.

关键词： inflammatory cytokine PGE2

Synthesis and characterization of NaYF4:Yb3+,Er3+@silica-N=folic acid nanocomplex for bioimaginable detecting MCF-7 breast cancer cells

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Journal of Rare Earths 2019年第11期37卷 1183-1187页

作者： Ha thi Phuong Tran Thu Huong Le thi Vinh Hoang thi Khuyen Do thi thao nguyen Thanh Huong Pham thi Lien Le Quoc Minh Institute of Materials Science Vietnam Academy of Science and Technology18 Hoang Quoc VietCau GiayHanoiViet Nam Graduate University of Science and Technology-Vietnam Academy of Science and Technology 18 Hoang Quoc VietCau GiayHanoiViet Nam Department of Chemistry Hanoi Medical University1-Ton That TungDong DaHanoiViet Nam Department of Chemistry Hanoi University of Mining and Geology18-Pho LienBac Tu LiemHanoiViet Nam Institute of Biotechnology Vietnam Academy of Science and Technology18-Hoang Quoc VietCau GiayHanoiViet Nam Duy Tan University K7/25 Quang TrungDa NangViet Nam

Upconversion nanophosphors are new promising nanomaterials to be used as biolabels for detection and imaging of cancer *** nanophosphors absorb long-wavelength excitation radiation in the infrared or near infrared region and emit shorter wavelength,higher energy radiation from ultraviolet to *** this paper,we studied the hydrothermal method and optical properties of the functionalized NaYF4:Yb^3+,Er^3+for biomedical *** synthesis,these NaYF4:Yb^3+,Er^3+nanophosphors were functionalized with aminosilanes and folic *** acid binds to the folate receptor on the surface of MCF-7 breast cancer cells and this binding promotes internalization of the nanophosphors via *** sizes of the functionalized NaYF4:Yb^3+,Er^3+@silica-N=FA(folic acid)nanophosphors can be controlled with length of the rod about 300-800 nm and diameter of the rod about 100-200 *** structure of NaYF4:Yb^3+,Er^3+is in hexagonal crystal *** photo luminescence(PL)spectra of the functionalized NaYF4:Yb^3+,Er^3+@silica-N=FA nanophosphors were *** nanophosphors emit in red color with the strongest band at 650 nm under 980 nm *** result can provide NaYF4:Er^3+,Yb^3+@silica-N=FA complex for developing fluorescence label and image tool in cancer biology and medicine.

关键词： Upconversion luminescence NaYF4:Yb^3+,Er^3+ Nanophosphors Bioimaging Cancer cell Rare earths

Designing Software to Analyze Sewing Process of Industrial Knitted Products

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Computer Systems Science & Engineering 2023年第2期44卷 1837-1852页

作者： Phan Thanh thao nguyen Thanh Hung Pham thi Le My nguyen Xuan Hiep Duy-Nam Phan Hanoi University of Science and Technology Hanoi10000Vietnam

In the textile industry,garment manufacturing contains four major pro-cesses containing cutting,sewing,ﬁnishing,and *** is the most crucial and intricate section,dealing with a large number of varied operations.A successful sewing process needs to be optimized regarding different factors,including time,sewing equipment,and skilled *** lineﬂow is combined by a set of operations with a particular *** utmost impor-tance of all garment industry is to arrange the workstations to minimize the num-ber of employees in order to produce at the best productive rate with the most reasonable cost,shortest time,and satisfying *** most garment factories,the production lines are balanced using the empirical judgment of the line *** the whole process the data of production time at each step,labor pro-ductivity,proper choices of equipment were always needed to calculate line efﬁ*** far as the issue is concerned,there has not been an academically sewing process analyzing software providing adequate data of sewing motions and sewing time as the credible input for the line balancing *** this goal,this paper presents the results of research on optimizing academically self-built software to analyze the sewing process of knitted products applied to industrial production using Java programming language on Google *** results achieved by the software are not only to analyze sewing products and the technological sewing process,calculate the sewing time on the machine but also analyze the sewing activities of workers into manipulations,movements,and motions to calculate the preparation time for two typical knitted products,namely,Polo-Shirt and T-Shirt with the case studies at General Textile Garment Joint Stock Company Hanoi and Star Fashion Company Limited.

关键词： Sewing motion sewing time GSD MTM polo-shirt T-shirt sewing process sewing operation analyzing software

Successful containment of a COVID-19 outbreak in Bach Mai Hospital by prompt and decisive responses

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Asian Pacific Journal of Tropical Medicine 2021年第5期14卷 234-235页

作者： Van Minh Hoang thi Phuong thao Tran thi Ngoc Phuong nguyen thi Hai Yen nguyen Quang Tuan nguyen Hanoi University of Public Health HanoiVietnam Bach Mai Hospital HanoiVietnam

In low-and middle-income countries,tertiary care hospitals frequently face an extremely heavy workload and shoulder an even heavier burden during the COVID-19 *** Mai Hospital(BMH),one of the leading tertiary hospitals in Vietnam,is on the frontline in the fierce battle against *** letter aims to describe how BMH tackles the COVID-19 outbreak in the hospitallevel battle,which could support policymakers in time-management and well-preparation for this unpredictable pandemic.

关键词： prompt income policy

In vivo anticancer activity of maesopsin 4—O— β—glucoside isolated from leaves of Artocarpus tonkinensis *** Gagnep

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Asian Pacific Journal of Tropical Medicine 2016年第4期9卷 343-348页

作者： Trinh thi Thuy Dao Duc thien Tran Quang Hung nguyen Thanh Tam nguyen thi Hoang Anh nguyen thi Nga nguyen thi Cuc Le Phuong Mai Tran Van Sung Domenico V.Delfino Do thi thao Institute of Chemistry.Vietnam Academy of Science and Technology Institute of Biotechnology Vietnam Academy of Science and Technology National Research Council Canada Measurement Science and Standards1200 Montreal RoadBuilding M-12.Ottatca.Ontario KIA OR6 Department of Medicine University of PerugiaPiazzale Gambuli.S.Andrea delle Fratte

Objective:To investigate the antitumor effect of maesopsin 4-O-β-glucoside(TAT2) isolated from the leaves of Artocarpus tonkinensis(***) *** ***:The antitumor activity of TAT2 was evaluated in Lewis lung carcinoma(LLC) tumor-bearing ***/c mice had tumors induced by implantation with 2× 10~6 LLC cells into the subcutaneous right posterior ***-bearing mice were treated orally with a range of doses of TAT2 and a standard drug,*** were observed for tumor growth and mortality *** was collected to determine hematological and biochemical *** TAT2 was isolated from an ethanolic extract of *** *** structure was determined by MS and NMR spectroscopy,and identified as *** compound did not show acute toxicity at the highest dose tested(2 OIK) mg/kg body weight).TAT2 exhibited antitumor activity by decreasing tumor growth,increasing the survival rale,and ameliorating some hematological and biochemical parameters at doses of 100 and 200 mg/kg body weight(P<0.05).Conclusions:These results indicate that TAT2 possesses clear antitumor *** to its bioavailability and low toxicity,and the fact that it could be isolated in a large scale from *** leaves,the compound shows promise as a potential anticancer drug.

关键词： Artocarpus tonkinensis BALB/c Hematological parameters Lewis lung carcinoma Maesopsin 4-0-β-glucoside

The COVID-19 pandemic in the ASEAN: A preliminary report on the spread, burden and medical capacities

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Asian Pacific Journal of Tropical Medicine 2020年第6期13卷 247-251页

作者： Minh Van Hoang Phuong thi Ngoc nguyen thao thi Phuong Tran Long Quynh Khuong An thi Minh Dao Huy Van nguyen Riyanti Djalante Hanh thi Tuyet Tran Hanoi University of Public Health HanoiVietnam Hanoi Medical University HanoiVietnam Graduate School of Public Health St.Luke's International UniversityTokyoJapan United Nations University-Institute for the Advanced Study of Sustainability(UNU-IAS) TokyoJapan

Objective:To provide preliminary descriptions of the spread,burden and related medical capacity characteristics of the ASEAN ***:We utilized the data from four main official databases,including WHO reports,the Statistics and Research of the Coronavirus Disease,and the Southeast Asia Program of the Center for Strategic and International *** spread of the COVID-19 pandemic,current burden and the COVID-19 medical response capacities were extracted before April 11,***:As of April 13,2020,the ASEAN countries reported19547 COVID-19 positive cases with 817 deaths(case-facility rate of 4.2%).Thailand was the first country in the region that reported having the COVID-19 cases,while Laos was the last to report confirmed COVID-19 *** for the number of deaths to double were between 8-12 *** the testing and treatment capacities,the number of PCR tests provided to the populations was the highest in Vietnam,followed by Singapore,Malaysia,and ***,the percentage of the population being tested was the highest in Brunei(2.31%),followed by Singapore(1.30%).Conclusions:A wide range of interventions were taken into practice in response to the outbreak with an effort of curbing the rise of this ***,special care should not be overlooked as the pandemic is placing a huge impact on the population and becomes increasingly *** tackle the spread of the pandemic in the region,the ASEAN countries should work together as one community to provide better responses to future pandemics and other transboundary public health challenges.

关键词： COVID-19 SARS-CoV-2 ASEAN South-East Asia Epidemiology