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Assessing genetic diversity of wild populations of Japanese flounderusing AFLP markers

Acta Oceanologica Sinica 2006年第3期25卷 82-89页

作者： XU Xiaofei ZHANG Quanqi WANG Zhigang QI Jie ZHANG Zhifeng BAO Zhenmin heisuke nakagawa Laboratory of Marine Genetics and Breeding Ocean University of China Qingdao 266003 China Faculty of Applied Biological Science Hiroshima University Higashi - Hiroshima 739 - 8528 Japan

Amplified fragment length polymorphism （AFLP） analysis was used to evaluate the genetic diversity of four wild geographical populations of Japanese flounder （Paralichthys olivaceus）. A total of 775 loci （58.32% of which was polymorphic） in the range between 100 and 1 300 base pairs were detected from 110 individuals using seven primer combinations. The percentage of polymorphic loci detected by single primer combination for each population was calculated, ranging from 19.59% to 53.33%. Genetic similarities within and among the populations were calculated from the binary matrices of presence - absence. Phylogenetic tree of four populations was constructed by using the UPGMA method using PHYLIP Version 3.5. According to intrapopulation genetic similarities, CW population displayed the highest genetic diversity value and KY population had the lowest genetic diversity value. The distance between CW and CF populations was the farthest, which was possibly resulted from the farthest distance of Weihai of Shandong and Fujian of China compared with the geographical distance between other locations of populations. The subpopulation differentiation value （G.,） is 0. 356 5, showing a certain extent of differentiation among the four geographical populations. AFLP technology was confirmed to be an effective tool to assess within- and among-population genetic diversity of Japanese flounder. The present survey provided significant insights for research in the Japanese flounder breeding program.

关键词： Paralichthys olivaceus geographical population AFLP genetic diversity

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Effect of dietary docosahexaenoic acid on lipogenesis and lipolysis in black sea bream,Acanthopagrus schlegeli

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Acta Oceanologica Sinica 2007年第1期26卷 112-121页

作者： JI Hong OM Ahamd Daud YOSHIMATSU Takao UMINO Testuya nakagawa heisuke FURUHASHI Makoto SAKAMOTO Shuichi College of Animal Science and Technology Northwest Agriculture and Forestry University Shaanxi 712100 China Graduate School of Biosphere Science Hiroshima University Higashi - hiroshima 739 - 8528 Japan Marine Finfish Production and Research Center Terengganu 22200 Malaysia Farming System Division National Research Institute of Aquaculture Fisheries Research Agency Mie 516 -0913 Japan Oriental Yeast Co Ltd Chiba 281 -0002 Japan

Hatchery-roared juvenile black sea breams are characterized by a low level of highly unsaturated fatty acids in their bodies, as compared with wild fish. To assess the effect of docosahaxaenoic acid （DHA） on lipegenic and lipelysis enzymes, one-year fish were roared on a casein-based purified diet and a DHA fortified diet （ 1.5% DHA ethyl ester/kg diet） for 60 d, followed with a period of 55 d for starvation. Dietary DHA was effectively incorporated into the fish body. Fortification of DHA depressed activities of glucose-6-phosphate dehydrogenase and NADP-isocitrate dehydrogenase as lipogenic enzymes in the hepatopancroas and intraperitoneal fat body. Carnitine palmitoyltransferase as lipolysis enzyme in the hepatopancreas was active in the DHA fortified fish. Starvation after feeding experiment induced increased carnitine palmitoyltransferase activity in both control and DHA fortified fish and the activity remained higher in the DHA fortified fish, while the monoenes were selectively consumed prior to highly unsaturated fatty acids. These results indicated that dietary DHA depressed lipogenesis and activated lipolysis.

关键词： DHA enzyme lipogenesis lipolysis black sea bream

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月桂酸酯对真鲷幼鱼脂质蓄积和生理活力的影响(英文)

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水产学报 2005年第6期29卷 804-810页

作者：吉红 Ahmad Daud Om 海野徹也吉松隆夫林雅弘中川平介浅野雅也中川敦史西北农林科技大学动物科技学院广岛大学大学院生物圈科学研究科 3. 国立水产综合研究中心养殖研究所 4. 宫崎大学农学部 5. 协和发酵工业株式会社水产研究所

在配合饲料中加入月桂酸酯(0.5%)饲喂真鲷稚鱼(0.3 g)以评价其对脂质蓄积及生理活力的影响。经过47 d饲养,对真鲷的生长情况、鱼体性状和体成份进行测定和分析,并通过施以麻醉和离水应激以评价其抗逆能力。结果表明,饲料中添加月桂酸酯... 详细信息

在配合饲料中加入月桂酸酯(0.5%)饲喂真鲷稚鱼(0.3 g)以评价其对脂质蓄积及生理活力的影响。经过47 d饲养,对真鲷的生长情况、鱼体性状和体成份进行测定和分析,并通过施以麻醉和离水应激以评价其抗逆能力。结果表明,饲料中添加月桂酸酯未影响生长,但提高了肌肉比率,降低了比肝重和腹腔脂肪组织比率。同时脂肪细胞缩小,肝脂及肝脂中中性脂肪比率下降,组织中DHA比率有上升趋势。另外,饲料月桂酸酯增强了真鲷肝功能和离水等抗逆能力,指出月桂酸酯的效果可能与组织中DHA比率上升有关。

关键词：月桂酸酯脂质蓄积 DHA 真鲷生理活力

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On the Numerical Modeling of the Deep Mantle Water Cycle in Global-Scale Mantle Dynamics:The Effects of the Water Solubility Limit of Lower Mantle Minerals

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Journal of Earth Science 2017年第4期28卷 563-577页

作者： Takashi nakagawa Department of Mathematical Science and Advanced Technology Japan Agency for Marine-Earth Science and TechnologyYokohama 236-0001Japan

Water is the most important component in Earth system evolution. Here, I review the current understanding of the fate of water in the mantle dynamics system based on high-pressure and temperature experiments, geochemical analyses, seismological and geomagnetic observations, and nu- merical modeling of both regional- and giobal-scale mantle dynamics. In addition, as a numerical ex- ample, I show that the water solubility of the deep mantle is strongly sensitive to global-scale water cir- culation in the mantle. In a numerical example shown here, water solubility maps as functions of tem- perature and pressure are extremely important for revealing the hydrous structures in both the mantle transition zone and the deep mantle. Particularly, the water solubility limit of lower mantle minerals should be not so large as ~100 ppm for the mantle transition zone to get the largest hydrous reservoir in the giobal-scale mantle dynamics system. This result is consistent with the current view of mantle water circulation provided by mineral physics, which is also found as a hydrous basaltic crust in the deep mantle and the water enhancement of the mantle transition zone simultaneously. In this paper, I also discuss some unresolved issues associated with mantle water circulation, its influence on the onset and stability of plate motion, and the requirements for developing Earth system evolution in mantle dy- namics simulations.

关键词： numerical modeling mantle convection water cycle water solubility limit.

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A weeding-duration model for Abies sachalinensis plantations in Hok-kaido, northern Japan

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Journal of Forestry Research 2013年第1期24卷 131-136页

作者： Masahiko nakagawa Doto Station Forestry Research InstituteHokkaido Research OrganizationShintoku-choHokkaido081-0038Japan

I developed a weeding-duration model for Sakhalin fir （Abies sachalinensis （Fr. Schmidt） Masters） plantations that employs a generalized linear model. The number of years following planting that weeding is necessary is the response variable, and elevation, slope steepness, maximum snow depth, annual precipitation, geology, soil, site index, slope aspect, and vegetation type are explanatory variables. Among the explanatory variables, geology, soil, slope aspect, and vegetation type are categorical data. A Poisson distribution is assumed for the response variable, with a log-link function. Elevation, slope steepness, maximum snow depth, annual precipitation, site index, and vegetation type had a significant effect on weeding duration. Among the eight models with the smallest Akaike information criterion （AIC）, I chose the model with no multicollinearity among the explanatory variables. The weeding-duration model includes site index, maximum snow depth, slope steepness （angle） and vegetation type as explanatory variables; elevation and annual precipitation were not included in the selected model because of multicollinearity with maximum snow depth. This model is useful for cost-benefit analyses of afforestation or reforestation with Abies sachalinensis.

关键词： Abies sachalinensis plantation snow depth site index weeding

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Promoting functional recovery by inhibition of repulsive guidance molecule-a after spinal cord injury

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Neural Regeneration Research 2018年第6期13卷 981-982页

作者： Hiroshi nakagawa Masahiko Takada Sobell Department of Motor Neuroscience and Movement Disorders Institute of Neurology University College London Systems Neuroscience Section Primate Research Institute Kyoto University

Spinal cord injury（SCI）leads to permanent disability with motor and sensory *** mature mammalian central nervous system（CNS）possesses a limited capacity to regenerate/regrow after injury.

关键词： Promoting functional recovery by inhibition of repulsive guidance molecule-a after spinal cord injury SCI

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Taming heterogeneous rhenium catalysis for the production of biomass-derived chemicals

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Chinese Chemical Letters 2020年第5期31卷 1071-1077页

作者： Keiichi Tomishige Yoshinao nakagawa Masazumi Tamura Department of Applied Chemistry School of EngineeringTohoku UniversityMiyagi 980-857Japan

Rhenium is one of important components for heterogeneous catalysts,which has been recently used for the catalytic reactions related to the production of biomass-derived chemicals such as deoxydehydration of vicinal OH groups,C-O hydrogenolysis,and hydrogenation of carboxylic acids,and so *** oxidation state of Re as a catalytic active species is strongly dependent on the catalytic *** control of the oxidation state of Re species on the catalysts is crucial on the catalyst development.

关键词： Rhenium Oxidation state Deoxydehydration Hydrogenolysis Hydrogenation

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Prediction of Photosynthetic Carbon Assimilation Rate of Individual Rice Leaves under Changes in Light Environment Using BLSTM-Augmented LSTM

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Computer Modeling in Engineering & Sciences 2022年第12期133卷 557-577页

作者： Masayuki Honda Kenichi Tatsumi Masaki nakagawa Graduate School of Bio-Applications and Systems Engineering Tokyo University of Agriculture and TechnologyKoganei City184-8588Japan Institute of Agriculture Tokyo University of Agriculture and TechnologyFuchu City183-0054Japan Institute of Engineering Tokyo University of Agriculture and TechnologyKoganei City184-8588Japan

A model to predict photosynthetic carbon assimilation rate(A)with high accuracy is important for forecasting crop yield and *** short-term memory(LSTM),a neural network suitable for time-series data,enables prediction with high accuracy but requires mesophyll *** addition,for practical use,it is desirable to have a technique that can predict A from easily available *** this study,we propose a BLSTM augmented LSTM(BALSTM)model,which utilizes bi-directional LSTM(BLSTM)to indirectly reproduce the mesophyll variables required for *** most significant feature of the proposed model is that its hybrid architecture uses only three relatively easy-to-collect external environmental variables—photosynthetic photon flux density(Q_(in)),ambient CO_(2) concentration(C_(a)),and temperature(T_(air))—to generate mesophyll CO_(2) concentration(C_(i))and stomatal conductance to water vapor(g_(sw))as intermediate ***,A is predicted by applying the obtained intermediate outputs to the learning ***,in this study,1)BALSTM(Q_(in),C_(a),T_(air))had a significantly higher A prediction accuracy than LSTM(Q_(in),C_(a),T_(air))in case of using only Q_(in),C_(a),and T_(air);2)BALSTMC_(i),g_(sw),which had C_(i) and g_(sw) as intermediate products,had the highest A prediction accuracy compared with other candidates;and 3)for samples where LSTM(Q_(in),C_(a),T_(air))had poor prediction accuracy,BALSTMC_(i),g_(sw)(Q_(in),C_(a),T_(air))clearly improved the ***,it was found that incorrect predictions may be formed when certain factors are not reflected in the data(e.g.,timing,cultivar,and growth stage)or when the training data distribution that accounts for these factors differs from the predicted data ***,a robust model should be constructed in the future to improve the prediction accuracy of A by conducting gasexchange measurements(including a wide range of external environmental values)and by increasing the

关键词： Hybrid prediction model assimilation rate leaf internal variables recurrent neural network fluctuating light environments rice

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Feature-aware regularization for sparse online learning

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Science China(Information Sciences) 2014年第5期57卷 65-85页

作者： OIWA Hidekazu MATSUSHIMA Shin nakagawa Hiroshi Graduate School of Information Science and Technology The University of Tokyo Information Technology Center The University of Tokyo

Learning a compact predictive model in an online setting has recently gained a great deal of *** combination of online learning with sparsity-inducing regularization enables faster learning with a smaller memory space than the previous learning *** optimization methods and learning algorithms have been developed on the basis of online learning with L1-regularization.L1-regularization tends to truncate some types of parameters,such as those that rarely occur or have a small range of values,unless they are emphasized in ***,the inclusion of a pre-processing step would make it very difficult to preserve the advantages of online *** propose a new regularization framework for sparse online *** focus on regularization terms,and we enhance the state-of-the-art regularization approach by integrating information on all previous subgradients of the loss function into a regularization *** resulting algorithms enable online learning to adjust the intensity of each feature’s truncations without pre-processing and eventually eliminate the bias of *** show theoretical properties of our framework,the computational complexity and upper bound of *** demonstrated that our algorithms outperformed previous methods in many classification tasks.

关键词： online learning supervised learning sparsity-inducing regularization feature selection sentiment analysis

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Reductive Conversion of Biomass-Derived Furancarboxylic Acids with Retention of Carboxylic Acid Moiety

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Transactions of Tianjin University 2021年第3期27卷 165-179页

作者： Yoshinao nakagawa Mizuho Yabushita Keiichi Tomishige Department of Applied Chemistry School of EngineeringTohoku University6-6-07 AobaAramakiAoba-kuSendai 980-8579Japan Research Center for Rare Metal and Green Innovation Tohoku University468-1AobaAramakiAoba-kuSendai 980-0845Japan

Catalytic reduction systems of 2-furancarboxylic acid(FCA)and 2,5-furandicarboxylic acid(FDCA)with H 2 without reduction of the carboxyl groups are *** and FDCA are produced from furfural and 5-hydroxymethylfurfural which are important platform chemicals in biomass *** ring hydrogenation to tetrahydrofuran-2-carboxylic acid(THFCA)and tetrahydrofuran-2,5-dicarboxylic acid(THFDCA)easily proceeds over Pd *** of one C–O bond in the furan ring produces 5-hydroxyvaleric acid(5-HVA)and 2-hydroxyadipic acid.2-Hydroxyvaleric acid is not produced in the reported systems.5-HVA can be produced as the lactone form(δ-valerolactone;DVL)or as the esters depending on the *** reactions proceed over Pt catalysts with good yields(~70%)at optimized *** of two C–O bonds in the furan ring produces valeric acid and adipic acid,the latter of which is a very important chemical in industry and its production from biomass is of high *** acid from FDCA can be produced directly over Pt-MoO_(x) catalyst,indirectly via hydrogenation and hydrodeoxygenation as one-pot reaction using the combination of Pt and acid catalysts such as Pt/niobium oxide,or indirectly via two-step reaction composed of hydrogenation catalyzed by Pd and hydrodeoxygenation catalyzed by iodide ion in acidic *** the two-step method can give good yield of adipic acid at present.

关键词： Biomass Hydrogenolysis Furanic compound Carboxylic acid

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