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Identification and Antimicrobial Susceptibility of Yersinia enterocoliticaFound in Chitterlings, Raw Milk and Swine Fecal Samples

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Advances in Microbiology 2018年第10期8卷 804-820页

作者： F. n. Drake S. Davis j. Khatiwada L. Williams north Carolina A & T State University North Carolina Research Campus Kannapolis NC USA

Foodborne illness is an escalating concern upon public health. The prevalence of Yersinia enterocolitica was assessed in chitterlings, raw milk and swine fecal from north Carolina. Uncleaned thirty chitterling samples procured from a local grocery store, forty-five swine fecal samples, and forty unpasteurized cow milk samples supplied by the University farm were evaluated for the presence of Y. enterocolitica. Isolates identified as presumptive positive were characterized as colonies with a pink or deep-red center on MacConkey and CIn agar, and verified further through polymerase chain reaction (PCR) for the presence of 16S rRnA gene for the Yersinia genera. Results showed that 4.4% swine fecal samples, 7.5% milk samples and 11.3% chitterling samples were presumptive positive for Y. enterocolitica by the direct plating method on selective agars. Of the thirty-chitterling samples examined by PCR for the 16S rRnA gene, 26% samples contained the identification gene for the bacteria of interest. After conducting virulence tests, the fecal samples were revealed as non-pathogenic. Only one of the milk samples were considered pathogenic and consisted of the following virulent genes: Yersinia heat-stable toxin (yst), invasion (inv), attachment invasion locus (ail), virulence regulon transcriptional activator (virF), Yersinia adehesin A (yadA), and the O:3 antigen gene (rfbC). Seven out of the eight (87.5%) chitterling samples were shown to be pathogenic. Disc diffusion was conducted to determine the antimicrobial susceptibility of the isolates. Over half (55.5%) of the antimicrobial agents were found effective, with isolates being completely susceptible to ciprofloxacin, kanamycin, trimethoprim, cefotaxime, and gentamycin. Ampicillin was determined to be least effective, where 84.6% of the samples presented resistance to the drug. Random amplified polymorphic DnA (RAPD) analysis and ERIC-PCR techniques were used to evaluate genetic similarity among the Yersinia isolates. Th

关键词： Yersinia enterocolitica Polymerase Chain Reaction Antimicrobial

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Molecular and compact four-quark states

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Chinese Physics C 2010年第9期34卷 1290-1293页

作者： A.Valcarce j.Vijande Departamento de Física Fundamental Universidad de Salamanca Departamento de Física Atómica Molecular y NuclearUniversidad de Valencia (UV) and IFIC (UV-CSIC)

We study charmonium （ccnn）,bottomonium （b bnn） and exotic （ccnn and bbnn） four-quark states by means of a standard non-relativistic quark potential *** look for possible bound *** them we are able to distinguish... 详细信息

关键词： tetraquarks quark-models charmonium exotics

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Restoration of energy level in the early phase of acute pediatric pancreatitis

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World journal of Gastroenterology 2017年第6期23卷 957-963页

作者： Dóra Mosztbacher nelli Farkas Margit Solymár Gabriella Pár judit Bajor ákos Szucs józsef Czimmer Katalin Márta Alexandra Mikó Zoltán Rumbus Péter Varjú Péter Hegyi Andrea Párniczky First Department of Pediatrics Semmelweis University Institute for Translational Medicine University of Pécs Institute for Bioanalyses University of Pécs Department of Gastroenterology First Department of MedicineUniversity of Pécs Department of Translational Medicine First Department of MedicineUniversity of Pécs First Department of Surgery Semmelweis University Hungarian Academy of Sciences-University of Szeged Momentum Gastroenterology Multidisciplinary Research Group Heim Pál Children’s Hospital

Acute pancreatitis (AP) is a serious inflammatory disease with rising incidence both in the adult and pediatric populations. It has been shown that mitochondrial injury and energy depletion are the earliest intracellular events in the early phase of AP. Moreover, it has been revealed that restoration of intracellular ATP level restores cellular functions and defends the cells from death. We have recently shown in a systematic review and meta-analysis that early enteral feeding is beneficial in adults; however, no reviews are available concerning the effect of early enteral feeding in pediatric AP. In this minireview, our aim was to systematically analyse the literature on the treatmentof acute pediatric pancreatitis. The preferred reporting items for systematic review(PRISMA-P) were followed, and the question was drafted based on participants, intervention, comparison and outcomes: P: patients under the age of twenty-one suffering from acute pancreatitis; I: early enteral nutrition (per os and nasogastric- or nasojejunal tube started within 48 h); C: nil per os therapy; O: length of hospitalization, need for treatment at an intensive care unit, development of severe AP, lung injury (including lung oedema and pleural effusion), white blood cell count and pain score on admission. Altogether, 632 articles (Pub Med: 131; EMBASE: 501) were found. After detailed screening of eligible papers, five of them met inclusion criteria. Only retrospective clinical trials were available. Due to insufficient information from the authors, it was only possible to address length of hospitalization as an outcome of the study. Our mini-meta-analysis showed that early enteral nutrition significantly(SD = 0.806, P = 0.034) decreases length of hospitalization compared with nil per os diet in acute pediatric pancreatitis. In this minireview, we clearly show that early enteral nutrition, started within 24-48 h, is beneficial in acute pediatric pancreatitis. Prospective studies and better pres

关键词： pediatric pancreatitis enteral nutrition nil per os diet ATP restoration length of hospitalization

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工程师必读工程问题和解答

工程师必读工程问题和解答

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1985年

作者：（美）康斯坦斯 j.D. 孙庆征译

本书内容有：机械零件强度，机器设计，齿轮传动装置等。

关键词：工程技术基础科学学科:习题

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Initial evaluations of a Gulf of Mexico/Caribbean ocean forecast system in the context of the Deepwater Horizon disaster

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Frontiers of Earth Science 2015年第4期9卷 605-636页

作者： Edward D. ZAROn Patrick j. FITZPATRICK Scott L. CROSS john M. HARDInG Frank L. BUB jerry D. WIGGERT Dong S. KO Yee LAU Katharine WOODARD Christopher n. K. MOOERS Department of Civil and Environmental Engineering Portland State University Portland OR 97207 USA Geosystems Research Institute Mississippi State University MSU Science & Technology Center Stennis Space Center MS 39529 USA nOAA national Coastal Data Development Center Stennis Space Center MS 29412 USA northern Gulf Institute Mississippi State University MSU Science & Technology Center Stennis Space Center MS 39529 USA unaffiliated retired from the Naval Oceanographic Office Stennis Space Center MS 39522 USA Department of Marine Science University of Southern Mississippi Stennis Space Center MS 39529 USA Oceanography Division Naval Research Laboratory Stennis Space Center MS 39529 USA

In response to the Deepwater Horizon （DwH） oil spill event in 2010, the naval Oceanographic Office deployed a nowcast-forecast system covering the Gulf of Mexico and adjacent Caribbean Sea that was designated Americas Seas, or AMSEAS, which is documented in this manuscript. The DwH disaster provided a challenge to the application of available ocean-forecast capabilities, and also generated a historically large observational dataset. AMSEAS was evaluated by four complementary efforts, each with somewhat different aims and approaches： a university research consortium within an Integrated Ocean Observing System （IOOS） testbed; a petroleum industry consortium, the Gulf of Mexico 3-D Operational Ocean Forecast System Pilot Prediction Project （GOMEX-PPP）; a British Petroleum （BP） funded project at the northern Gulf Institute in response to the oil spill; and the navy itself. Validation metrics are presented in these different projects for water temperature and salinity profiles, sea surface wind, sea surface temperature, sea surface height, and volume transport, for different forecast time scales. The validation found certain geographic and time biases/errors, and small but systematic improvements relative to earlier regional and global modeling efforts. On the basis of these positive AMSEAS validation studies, an oil spill transport simulation was conducted using archived AMSEAS nowcasts to examine transport into the estuaries east of the Mississippi River. This effort captured the influences of Hurricane Alex and a non-tropical cyclone off the Louisiana coast, both of which pushed oil into the western Mississippi Sound, illustrating the importance of the atmospheric influence on oil spills such as DwH.

关键词： Gulf of Mexico Deepwater Horizon ocean forecasting skill assessment

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Reflections on Long-Term Development and Use of Automated Scoring Technology in a Sport (Modified Boxing) Context

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World journal of Engineering and Technology 2017年第3期5卷 455-480页

作者： Paul Perkins Richard j. n. Helmer Colin Mackintosh Allan G. Hahn University of Canberra Research Institute for Sport and Exercise Canberra Australia Boxing Australia Limited Canberra Australia Superinteractive Pty Ltd Geelong Australia AppSen Canberra Australia Queensland Academy of Sport Centre of Excellence for Applied Sport Science Research Brisbane Australia Griffith University School of Engineering Brisbane Australia Australian Institute of Sport Canberra Australia Victoria University College of Engineering and Science Melbourne Australia

Technology is being increasingly used to aid judging in sport, but its employment as the primary means of scoring is rare. We have developed and implemented a fully automated scoring system in the context of a modified, low-risk form of boxing. The system, which requires contestants to wear vests and gloves incorporating sensor fabrics, has been used in multiple settings over the past five years. During that period, it has undergone progressive iteration guided by action research methodology. Here, we summarise that iteration, reflect on present status and identify possible future directions. We have found that concept of automated scoring has wide appeal, and the wearable technology is almost universally considered comfortable. nevertheless, some issues remain to be addressed. Use of the technology requires considerable prior and subsequent commitment of time. Apparently valid contacts occasionally fail to score. Causative factors include the configuration of electrical circuitry in the vests and deterioration of that circuitry with repeated vest use and washing. Also, false positive scores are sometimes generated by vest self-shorting and effects of sweat. Many contestants adopt unorthodox styles aimed at exploiting the characteristics of the automated scoring methodology, affecting the aesthetics of the modified sport. There is an expectation that technologically-based scoring should have much greater accuracy than human.j.dging, and should be essentially fail-proof. Disillusionment can occur in?situations where this expectation is not met. We have identified potential?solutions to all the existing issues, with some now being actively explored. Continuation of the quest seems justified by popular dissatisfaction with subjective human.j.dging of boxing and other sports, but we have come to realise that purely technological judging can introduce unforeseen complexities. Our observations could be relevant to various sports interested in the notion of technological j

关键词： Automated Scoring Technology Box’Tag Smart Fabric Sport Technology Wearable Sensors

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ORIEnTATIOn VARIAnT SELECTIOn AnD ITS EFFECTInG FACTORS In TITAnIUM SHEET

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Acta Metallurgica Sinica(English Letters) 1996年第6期9卷 601-605页

作者： Z.S. Zhu n.P.Chen.j.L. Gu and M.G. Yan(1)Institute of Aeronautical Materials, P.O. Box 8115, Beijing 100095, China 2)Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, ) Beijing Inst of Aeronautical Materials Beijing China

By means of crystallite orientation distribution function (ODF) analysis, the influence on orientation variant selection of cold rolling reductions, recrystallization annealing and cooling rates during α→β→α cyclic phase transformation treatments in cold rolled titanium sheet has been investigated. The results show that, cold rolling reduction and annealing treatment both have a great effect on the formation of phase transformation *** variant selection occurs in cold rolled and recrystallization annealed titanium sheet which resulte in a more randomly distributed *** phase transformation texture formed α→β→α transformation is inherited after 3 cycles of α→β→α phase transformation. The cooling rates during phase transformation have little effect on orientation variant selection.

关键词： texture titanium phase transformation orientation variant selection ODF

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产β-葡萄糖苷酶乳酸菌益生特性研究

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食品与发酵工业 2022年第15期48卷 85-90页

作者：冯程程蔡子哲陈琼欧仕益谢小冬汪勇 MARTIn.j.T REAnEY 张宁暨南大学食品科学与工程系广东广州510632 广东省油料生物炼制与营养安全国际联合研究中心广东广州510632 暨南大学-萨斯喀彻温大学“油料生物炼制与营养”联合实验室广东广州510632 广州市微生物研究所有限公司广东广州510663 加拿大萨斯喀彻温大学农业与生物资源学院加拿大萨斯卡通S7N5A8

研究了3株乳酸菌在不同碳源诱导下产β-葡萄糖苷酶的能力,并评价了其益生特性。研究发现,3株乳酸菌产生的β-葡萄糖苷酶同时具有水解烷基糖苷和芳香基糖苷的能力,纤维二糖诱导能显著提高β-葡萄糖苷酶活力。坚强肠球菌(Enterococcus dur... 详细信息

研究了3株乳酸菌在不同碳源诱导下产β-葡萄糖苷酶的能力,并评价了其益生特性。研究发现,3株乳酸菌产生的β-葡萄糖苷酶同时具有水解烷基糖苷和芳香基糖苷的能力,纤维二糖诱导能显著提高β-葡萄糖苷酶活力。坚强肠球菌(Enterococcus durans)GW18275在纤维二糖为碳源时有最大β-葡萄糖苷酶活力(16.02 U/mL)。模拟胃肠液耐受试验结果表明,3株乳酸菌活菌数保持在10^(6) CFU/mL以上。植物乳植杆菌(Lactiplantibacillus plantarum)C5在3 g/L胆盐培养3 h有最大存活率(83.70±6.21)%,对二甲苯和乙酸乙酯的表面疏水性最佳。植物乳植杆菌(Lactiplantibacillus plantarum)C1有最高的自聚集率(80.72±1.58)%。此外,3株菌均无溶血性,对头孢菌素类、β-酰胺类及氨基糖苷类抗生素有不同的药敏性,对四环素均敏感。3株乳酸菌具有较高的β-葡萄糖苷酶活性,作为功能性益生菌候选物具有良好的益生特性,可为日后开发高产β-葡萄糖苷酶益生制剂提供基础。

关键词： β-葡萄糖苷酶酶活力乳酸菌益生特性模拟胃肠液

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Antiferroelectricity in ZrO2and Ferroelectricity in Zr, Al, La Doped HfO2nanoparticles

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Advances in Materials Physics and Chemistry 2020年第2期10卷 27-38页

作者： A. T. Apostolov I. n. Apostolova j. M. Wesselinowa University of Architecture Civil Engineering and Geodesy Sofia Bulgaria University of Forestry Sofia Bulgaria University of Sofia Sofia Bulgaria

The dependence of the polarization P in Hf1-xZrxO2 nanoparticles on electric field, dopant concentration x, size and temperature are studied using the transverse Ising model and the Green’s function method. Pure ZrO2 shows at high electric fields an antiferroelectric behavior. Pure HfO2 is a linear dielectric in the monoclinic phase. With increasing ZrO2 content the of HZO shows a ferroelectric behavior. The composition dependence x of the remanent polarization Pr(x)?has a maximum for x = 0.5. For x = 0, pure HfO2, and x = 1, pure ZrO2, Pr=0.?P increases with decreasing HZO nanoparticle size. The influence of Al and La doping on Pr?in HfO2 nanoparticles is also studied. The exhibiting of the ferroelectricity in ion doped HfO2 is due to a phase transformation and to an internal strain effect. The observed results are in good qualitative agreement with the experimental data.

关键词： ZrO2 nanoparticles Zr Al La Doped HfO2 nanoparticles Electric Properties Microscopic Model Green’s Function

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苦寒的拂晓限制战略武器会谈内幕

苦寒的拂晓限制战略武器会谈内幕

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1974年

作者：（美）j.纽豪斯齐沛合译

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