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f?rster resonance energy transfer reveals phillygenin and swertiamarin concurrently target AKT on different binding domains to increase the anti-inflammatory effect

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Chinese Chemical Letters 2024年第5期 233-238页

作者： Xiaoyao ma Jinling Zhang Ge fang He Gao Jie Gao Li fu Yuanyuan Hou Gang Bai State Key Laboratory of Medicinal Chemical Biology College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research Nankai University College of Life Health Dalian University Dalian fusheng Pharmaceutical Co. Ltd.

The clinical benefit of combination therapy is significant, but it is not easy to define the mechanism of complexity and diversity. Previous studies illustrate that phillygenin(Phi) binds in the allosteric inhibit pocket of protein kinase B(AKT), and swertiamarin(Swe) acts on the pleckstrin homology(PH) domain of AKT. However, the combined synergistic effect of relieving the inflammatory response has yet to be elucidated. Based on high sensitivity, specificity and fast-responsibility fluorescent sensors, the f?rster resonance energy transfer(fRET) technique offers a route to provide clear insights into physiological and pathological processes. In the study, molecular docking, the fluorescent probes of Phi and Swe for fRET were designed and synthesized. fRET analysis shown that Swe and Phi concurrently acted on the PH domain and allosterically inhibited pocket of AKT, respectively. The combination of Swe and Phi significantly increased the heat stability of AKT and decreased protease-induced degeneration. In lipopolysaccharides(LPS)-induced mice and cells, the combination arrested AKT activation, nuclear factor kappa-B(Nf-κB)phosphorylation, and the expression of tumor necrosis factor-α(TNf-α), interleukin(IL)-6 and IL-8. In conclusion, fRET revealed Phi and Swe concurrently targeted AKT on different domains and the combination of Phi and Swe enhanced the anti-inflammatory effect.

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石墨烯-金纳米材料修饰电极用于L-酪氨酸的检测

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高等学校化学学报 2024年第4期45卷 10-18页

作者：马勤政王伟梁旭婷河北工业大学电子信息工程学院天津300401 天津市电子材料与器件重点实验室天津300401

采用一种绿色简单的电化学方法将还原氧化石墨烯-金纳米复合薄膜共沉积到玻碳电极(GCE)上,作为传感器用于L-酪氨酸(L-Tyr)的检测.采用扫描电子显微镜(SEM)、循环伏安法(CV)和电化学阻抗谱(EIS)对修饰电极进行了表征.采用差分脉冲伏安法(... 详细信息

采用一种绿色简单的电化学方法将还原氧化石墨烯-金纳米复合薄膜共沉积到玻碳电极(GCE)上,作为传感器用于L-酪氨酸(L-Tyr)的检测.采用扫描电子显微镜(SEM)、循环伏安法(CV)和电化学阻抗谱(EIS)对修饰电极进行了表征.采用差分脉冲伏安法(DPV)研究了0.1 mol/L磷酸盐缓冲溶液中L-Tyr在修饰电极上的电化学行为,发现L-Tyr在修饰电极上的伏安响应比裸GCE明显提高.对复合薄膜的厚度、支撑电解质的p H值、沉积电位和积累时间进行了优化;在最佳实验条件下, L-Tyr的氧化峰电流在0.1~50μmol/L及50~1000μmol/L浓度范围内呈现良好的线性关系.该传感器的检出限为50 nmol/L,灵敏度为0.553μA·μmol·L^(-1),具有良好的重复性、稳定性和抗干扰性.

关键词：电化学还原氧化石墨烯金纳米粒子共沉积 L-Tyr传感器

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超构表面:设计原理与应用挑战(特邀)

中国激光 2024年第1期51卷 259-279页

作者：马耀光高宇斌浙江大学极端光学技术与仪器全国重点实验室光电科学与工程学院先进光子学研究中心嘉兴研究院智能光子研究中心浙江大学杭州国际科创中心浙江杭州310027

作为一种二维形式的超构材料,超构表面允许以前所未有的自由度对光的振幅、相位、偏振等维度进行灵活高效的调控,有望突破传统光学的限制,实现低成本、高性能、超轻超薄、功能新颖的新型光学元件,近年来引起了学术界和产业界越来越浓厚... 详细信息

作为一种二维形式的超构材料,超构表面允许以前所未有的自由度对光的振幅、相位、偏振等维度进行灵活高效的调控,有望突破传统光学的限制,实现低成本、高性能、超轻超薄、功能新颖的新型光学元件,近年来引起了学术界和产业界越来越浓厚的研究兴趣。从物理机理、相位调控到正向设计方法,系统回顾了超构表面的设计原理。介绍了这些机理如何用于实现丰富的应用,包括功能复用、宽带宽、大视场、多层级联、非局域超构表面等,涵盖了最主要和最新的发展方向。最后,讨论了超构表面在走向实用化的道路上所面临的器件设计和加工制造上的挑战,并对领域未来的发展进行展望。

关键词：材料超构表面超构透镜成像光学全息复用消色差成像

Heme oxygenase 1-mediated ferroptosis in Kupffer cells initiates liver injury during heat stroke

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Acta Pharmaceutica Sinica B 2024年第9期14卷 3983-4000页

作者： Ru Li Riqing Wei Chenxin Liu Keying Zhang Sixiao He Zhifeng Liu Junhao Huang Youyong Tang Qiyuan An Ligen Lin Lishe Gan Liying Zhao Xiaoming Zou fudi Wang Yuan Ping Qiang ma The Seventh Affiliated Hospital Southern Medical UniversityFoshan 528244China Department of Biopharmaceutics School of Laboratory Medicine and BiotechnologySouthern Medical UniversityGuangzhou 510000China Medical Critical Care Medicine General Hospital of Southern Theatre Command of PLAGuangzhou 510000China Guangdong Branch Center National Clinical Research Center for Geriatric Diseases(Chinese PLA General Hospital)Guangzhou 510000China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoAvenida da UniversidadeTaip1Macao 999078China School of Pharmaceutical Sciences Zhejiang Chinese Medical UniversityHangzhou 311402China Department of General Surgery Nanfang Hospitalthe First School of Clinical MedicineSouthern Medical UniversityGuangzhou 510000China The fourth Affiliated Hospital the First Affiliated HospitalSchool of Public HealthInstitute of Translational MedicineCancer CenterState Key Laboratory of Experimental HematologyZhejiang University School of MedicineHangzhou 310000China The first Affiliated Hospital the Second Affiliated HospitalBasic Medical SciencesSchool of Public HealthHengyang Medical SchoolUniversity of South ChinaHengyang 421200China College of Pharmaceutical Sciences Zhejiang UniversityHangzhou 310000China Liangzhu Laboratory Zhejiang University Medical CenterHangzhou 310000China Guangdong Provincial Key Laboratory of Immune Regulation and Immunotherapy School of Laboratory Medicine and BiotechnologySouthern Medical UniversityGuangzhou 510000China

With the escalating prevalence of global heat waves,heat stroke has become a prominent health concern,leading to substantial liver *** other forms of liver injury,heat strokeinduced damage is characterized by heat cytotoxicity and heightened inflammation,directly contributing to elevated mortality *** clinical assessments have identified elevated bilirubin levels as indicative of Kupffer cell dysfunction,their specific correlation with heat stroke liver injury remains *** hypothesis proposes the involvement of Kupffer cell ferroptosis during heat stroke,initiating IL-1bmediated *** single-cell RNA sequencing of murine macrophages,a distinct and highly susceptible Kupffer cell subtype,Clec4ft/CD206t,emerged,with heme oxygenase 1(HMOX-1)playing a pivotal ***,heat-induced HMOX-1,regulated by early growth response factor 1,mediated ferroptosis in Kupffer cells,specifically in the Clec4f t/CD206 t subtype(KC2),activating phosphatidylinositol 4-kinase beta and promoting PI4P *** cascade triggered NLRP3 inflammasome activation and maturation of *** findings underscore the critical role of targeted therapy against HMOX-1 in ferroptosis within Kupffer cells,particularly in Clec4f t/CD206 t *** an approach has the potential to mitigate inflammation and alleviate acute liver injury in the context of heat stroke,offering a promising avenue for future therapeutic interventions.

关键词： Heat stroke Liver injury Kupffer cells ferroptosis Heme oxygenase 1 Phosphatidylinositol 4-kinase beta NLRP3 Early growth response factor 1

Antagonistic maDS-box transcription factors SEEDSTICK and SEPALLATA3 form a transcriptional regulatory network that regulates seed oil accumulation

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Journal of Integrative Plant Biology 2024年第1期66卷 121-142页

作者： Shuangcheng He Yuanchang Min Zijin Liu fang Zhi Rong ma Ankang Ge Shixiang Wang Yu Zhao Danshuai Peng Da Zhang Minshan Jin Bo Song Jianjun Wang Yuan Guo Mingxun Chen State Key Laboratory for Crop Stress Resistance and High‐Efficiency Production National Yangling Agricultural Biotechnology&Breeding CenterShaanxi Key Laboratory of Crop HeterosisCollege of AgronomyNorthwest A&F UniversityYangling 712100China State Key Laboratory for Crop Stress Resistance and High‐Efficiency Production Shaanxi Key Laboratory of AppleCollege of HorticultureNorthwest A&F UniversityYangling 712100China

Transcriptional regulation is essential for balancing multiple metabolic pathways that influence oil accumulation in *** far,the transcriptional regulatory mechanisms that govern seed oil accumulation remain largely ***,we identified the transcriptional regulatory network composed of maDS-box transcription factors SEEDSTICK(STK)and SEPALLATA3(SEP3),which bridges several key genes to regulate oil accumulation in *** found that STK,highly expressed in the developing embryo,positively regulates seed oil accumulation in Arabidopsis(Arabidopsis thaliana).furthermore,we discovered that SEP3 physically interacts with STK in vivo and in *** oil content is increased by the SEP3 mutation,while it is decreased by SEP3 *** chromatin immunoprecipitation,electrophoretic mobility shift assay,and transient dual-luciferase reporter assays showed that STK positively regulates seed oil accumulation by directly repressing the expression of MYB5,SEP3,and SEED fATTY ACID REDUCER 4(SfAR4).Moreover,genetic and molecular analyses demonstrated that STK and SEP3 antagonistically regulate seed oil production and that SEP3 weakens the binding ability of STK to MYB5,SEP3,and ***,we demonstrated that TRANSPARENT TESTA 8(TT8)and ACYL-ACYL CARRIER PROTEIN DESATURASE 3(AAD3)are direct targets of MYB5 during seed oil accumulation in ***,our findings provide the transcriptional regulatory network antagonistically orchestrated by STK and SEP3,which fine tunes oil accumulation in seeds.

关键词： seed oil accumulation SEP3 STK transcription factor transcriptional regulation

基于轻量化的DeepLabV3+遥感图像地物分割方法

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液晶与显示 2024年第8期39卷 1001-1013页

作者：马静郭中华马志强马小艳李迦龙宁夏大学电子与电气工程学院宁夏银川750021 宁夏大学沙漠信息智能感知重点实验室宁夏银川750021

针对DeepLabV3+在遥感图像地物分割中出现的细节信息丢失、类别不均衡等问题引起的误差,提出一种基于轻量化网络的DeepLabV3+遥感图像地物分割方法。首先,使用MobileNetV2替换原始基准网络中的骨干网络,提高训练效率并减少模型的复杂度... 详细信息

针对DeepLabV3+在遥感图像地物分割中出现的细节信息丢失、类别不均衡等问题引起的误差,提出一种基于轻量化网络的DeepLabV3+遥感图像地物分割方法。首先,使用MobileNetV2替换原始基准网络中的骨干网络,提高训练效率并减少模型的复杂度。其次,增大ASPP结构中空洞卷积的膨胀率,并在ASPP最后一层使用最大池化,有效地捕获不同尺度的上下文信息,同时在ASPP每个分支中引入SE注意力机制,并在提取浅层特征之后引入ECA注意力机制,提高模型对不同类别和细节的感知能力。最后,使用加权的Dice-focal联合损失函数进行优化,处理类别不均衡的问题。将改进的模型分别在CCf和华为昇腾杯竞赛数据集上进行验证,实验结果表明,本文所提出的方法相较于原始DeepLabV3+模型在两种测试集上的各个指标均有不同程度的提高。其中,mIoU达到了73.47%、63.43%,分别提高了3.24%和15.11%;准确率达到了88.28%、86.47%,分别提高了1.47%和7.83%;f1指数达到了84.29%和77.04%,分别提高了3.86%和13.46%。改进后的DeepLabV3+模型可以更好地解决细节信息丢失和类别不均衡的问题,提高遥感图像地物分割的性能和准确性。

关键词： MobileNetV2 空洞卷积注意力机制损失函数

Blastic plasmacytoid dendritic cell neoplasm:Two case reports

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World Journal of Clinical Oncology 2024年第9期15卷 1207-1214页

作者： Yi-Qian ma Zhan Sun Yu-Mei Li Hui Xu Department of Dermatology The Affiliated Hospital of Jiangsu UniversityZhenjiang 212000Jiangsu ProvinceChina

BACKGROUND Blastic plasmacytoid dendritic cell tumor(BPDCN)is a rare and highly invasive lymphohematopoietic tumor that originates from plasmacytoid dendritic *** has an extremely poor *** lesions are usually the first manifestation of BPDCN,although the tumor may also invade the bone marrow,lymph nodes,peripheral blood,and other parts of the body,leading to several other manifestations,requiring further differentiation through skin biopsy and *** SUMmaRY In the present paper,the cases of 2 patients diagnosed with BPDCN are *** immunohistochemistry analysis of these 2 patients revealed positivity for CD4,CD56,and ***,no standard chemotherapy regimen is available for ***,intensive therapy for acute lymphoblastic leukemia was applied as the treatment method for these 2 *** Although allogeneic bone marrow transplantation could be further effective in prolonging the median survival the ultimate prognosis was *** treatment modalities tailored for elderly patients will help prolong survival.

关键词： Blastic plasmacytoid dendritic cell neoplasm Skin CD4 CD56 CD123 Venetoclax Case report

mapping of powdery mildew resistance genes transferred to common wheat from wild emmer wheat revealed three functional Pm60 haplotypes

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The Crop Journal 2024年第2期12卷 540-548页

作者： Wenxin Wei Nannan Liu Shengnan Zhang Jing Zhang Wei Pan Xiaoming Xie Zuhuan Yang Junna Sun Jun ma Zhaorong Hu Weilong Guo Qiaoling Luo Jingzhong Xie fei He Yinghui Li Chaojie Xie Qixin Sun Key Laboratory of Crop Heterosis and Utilization(MOE)/Key Laboratory of Crop Genetic Improvement China Agricultural UniversityBeijing 100193China Institute of Advanced Agricultural Science Peking UniversityWeifang 261200ShandongChina Institute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101China Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Institute of Evolution University of HaifaMt.CarmelHaifa 3498838Israel

Powdery mildew(PM),caused by Blumeria graminis ***(Bgt),is one of the destructive wheat diseases *** emmer wheat(Triticum turgidum ***,WEW),a tetraploid progenitor of common wheat,is a valuable genetic resource for wheat disease resistance breeding *** developed three hexaploid pre-breeding lines with PM resistance genes derived from three WEW *** resistant pre-breeding lines were crossed with susceptible common wheat *** in the f2populations were 3 resistant:1 susceptible,suggesting a single dominant allele in each resistant *** of the resistance gene in each line indicated a single locus on the long arm of chromosome 7A,at the approximate location of previously cloned Pm60 from *** sequencing revealed three different Pm60 haplotypes(Hap 3,Hap 5,and Hap 6).Co-segregating diagnostic markers were developed for identification and selection of each *** resistance function of each haplotype was verified by the virus-induced gene silencing(VIGS).Common wheat lines carrying each of these Pm60 haplotypes were resistant to most Bgt isolates and differences in the response arrays suggested allelic variation in response.

关键词： Alleles Blumeria graminus marker-assisted selection Molecular marker Triticum dicoccoides

Genome-wide association study of seedling nitrogen-use efficiency-associated traits in common wheat(Triticum aestivum L.)

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The Crop Journal 2024年第1期12卷 222-231页

作者： Huawei Shi Weichong Wang Lifeng Gao Jirong Wu Chengmei Hu Huishu Yan Yugang Shi Ning Li Youzhi ma Yongbin Zhou Zhaoshi Xu Jun Chen Wensi Tang Kai Chen Daizhen Sun Yuxiang Wu Ming Chen College of Agriculture Shanxi Agricultural UniversityJinzhong 030801ShanxiChina National Key facility for Crop Gene Resources and Genetic Improvement Key Laboratory of Biology and Genetic Improvement of Triticeae CropsMinistry of AgricultureInstitute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Nitrogen(N)fertilizer application is essential for crop-plant growth and *** genetic loci associated with N-use efficiency(NUE)could increase wheat yields and reduce environmental pollution caused by *** subjected a panel of 389 wheat accessions to N and chlorate(a nitrate analog)treatments to identify quantitative trait loci(QTL)controlling NUE-associated traits at the wheat seedling *** the panel with a 660K single-nucleotide polymorphism(SNP)array,we identified 397 SNPs associated with N-sensitivity index and chlorate inhibition *** SNPs were merged into 49 QTL,of which eight were multi-environment stable QTL and 27 were located near previously reported QTL.A set of 135 candidate genes near the 49 QTL included TaBOX(f-box family protein)and TaERf(ethylene-responsive transcription factor).A Tabox mutant was more sensitive to low-N stress than the wild-type *** developed two functional markers for Hap 1,the favorable allele of TaBOX.

关键词： Wheat NUE Genome-wide association study Nitrogen sensitive index Chlorate inhibition rate