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Candidate genes conferring ethylene-response in cultivated peanuts determined by BSA-seq and fine-mapping

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The Crop Journal 2024年第3期12卷 856-865页

作者： Yanyan Tang Zhong Huang Shaohui Xu Wenjie Zhou Jianjun Ren Fuxin Yu Jingshan wang Wujun ma Lixian Qiao College of Agronomy Qingdao Agricultural UniversityQingdao 266109ShandongChina Murdoch University Food Futures InstitutePerthWA 6150Australia The Characteristic Laboratory of Crop Germplasm Innovation and Application Provincial Department of EducationCollege of AgronomyQingdao Agricultural UniversityQingdao 266109ShandongChina Academy of Dongying Efficient Agricultural Technology and Industry on Saline and Alkaline Land in Collaboration with Qingdao Agricultural University Dongying 257347ShandongChina

Ethylene plays essential roles in plant growth,development and stress *** ethylene signaling pathway and molecular mechanism have been studied extensively in Arabidopsis and rice but limited in ***,we established a sand-culture method to screen pingyangmycin mutagenized peanut lines based on their specific response to ethylene(“triple response”).An ethylene-insensitive mutant,inhibition of peanut hypocotyl elongation 1(iph1),was identified that showed reduced sensitivity to ethylene in both hypocotyl elongation and root *** bulked segregant analysis sequencing,a major gene related to iph1,named AhIPH1,was preliminarily mapped at the chromosome Arahy.01,and further narrowed to a 450-kb genomic region through substitution mapping strategy.A total of 7014 genes were differentially expressed among the ACC treatment through RNA-seq analysis,of which only the Arahy.5BLU0Q gene in the candidate mapping interval was differentially expressed between WT and mutant *** sequence variations,functional annotation and transcriptome analysis revealed that a predicated gene,Arahy.5BLU0Q,encoding SNF1 protein kinase,may be the candidate gene for *** gene contained two single-nucleotide polymorphisms at promoter region and was more highly expressed in iph1 than *** findings reveal a novel ethylene-responsive gene,which provides a theoretical foundation and new genetic resources for the mechanism of ethylene signaling in peanuts.

关键词： Ethylene-insensitive Hypocotyl elongation AhIPH1 Candidate gene Genetic resources

Spatiotemporal variation and evaluation of agriculture green development: a case study of Hainan Province, China

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Frontiers of Agricultural Science and Engineering 2024年第1期11卷 83-99页

作者： Tianxiang YU Jichen ZHOU Lin ma Fusuo ZHANG Zed RENGEL William JDAVIES Jianbo SHEN Department of Plant Nutrition College of Resources and Environmental SciencesCenter for ResourcesEnvironment and Food SecurityKey Laboratory of Plant-Soil interactionsMOENational Academy of Agriculture Green DevelopmentChina Agricultural UniversityBeijing 100193China Sanya Institute of China Agricultural University Sanya 572025China Soil Physics and Land management Group Wageningen UniversityP.O.Box 476700 AA Wageningenthe Netherlands Key Laboratory of Agricultural water Resources Center for Agricultural Resources ResearchInstitute of Genetic and Developmental BiologyChinese Academy of SciencesShijiazhuang 050021China Soil Science and Plant Nutrition UWA School of Agriculture and EnvironmentThe University of Western AustraliaPerth WA 6009Australia Institute for Adriatic Crops and Karst Reclamation 21000 SplitCroatia Lancaster Environment Centre University of LancasterLancaster LA14YQUK

The realization of green and sustainable development of agriculture is the common pursuit all over the world. Agriculture green development(AGD)program has been proposed as a sustainable development strategy in China,but insufficient is known about the quantitative evaluation of spatiotemporal variation in AGD at the regional scale. This study aimed to assess spatiotemporal patterns in AGD at the county/city-based regional level. For this purpose, a systematic index evaluation system was developed to assess the performance of socioeconomic, food production and environmental components in a key economic region(Hainan Province) of China. Hainan improved its AGD index(representing the overall performance toward achieving AGD) from 38.8 in 1988 to 40.9 in 2019. The socioeconomic development and agricultural productivity have improved with time;environmental quality declined due to overuse of chemicals from 1988 to 2013, but steadily improved after 2013, indicating positive effects of reducing chemical input. There was a higher AGD index in the coastal vs. central regions and the southern vs. northern regions. Scenarios featuring improved nutrient management or optimized diet structure and reduced waste improved economic benefits and social productivity while concurrently reducing environmental degradation. These results provide new insights for the future development of green and sustainable agriculture and formulation of agricultural policies in Hainan Province of China and even other developing countries that are facing or will soon face similar challenges.

关键词： Agriculture environmental impact green and sustainable development Hainan Province index evaluation system

Advances in photothermal conversion of carbon dioxide to solar fuels

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能源化学:英文版 2023年第8期83卷 62-78,I0004页

作者： wa Gao Yinwen Li Dequan Xiao Ding ma College of Bioscience and Resources Environment Beijing University of AgricultureBeijing 102206China College of Chemistry and Molecular Engineering and College of Engineering Peking UniversityBeijing 100871China Center for Integrative materials Discovery Department of Chemistry and Chemical and Biomedical EngineeringUniversity of New HavenWest HavenCT 06516USA

Converting carbon dioxide(CO_(2)) into useful fuels or chemical feedstocks is important for achieving peak carbon emission and carbon ***,photothermal catalysis has been extensively studied and applied due to its advantages over traditional heat-driven *** this review,we focus on photothermal catalysis of thermodynamically uphill reactions that convert CO_(2)into value-added *** first introduce the fundamentals of photothermal catalysis for CO_(2)reduction,including the definition and classification of photothermal catalysis,followed by their photothermal conversion *** structure design of different types of photothermal catalysts is *** superior performance of photothermal catalytic conversion of CO_(2)is illustrated and discussed,including improved CO_(2)activation,tunable selectivity towards different solar fuel products,and resistance to sintering and ***,the perspectives and challenges in this cutting-edge field are presented with the aim of advancing understanding of the underlying mechanisms and inspiring rational design of photothermal catalysts for highly efficient solar-to-fuel conversion.

关键词： Solarenergy Photothermal catalysis CO_(2)reduction Catalyst design Performance improvement

Protein-activated atomic layer deposition for robust crude-oil-repellent hierarchical nano-armored membranes

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Science Bulletin 2024年第2期69卷 218-226页

作者： Xiaobin Yang Pan Sun Yajie Wen Anil U.mane Jeffrey W.Elam Jun ma Shaomin Liu Seth B.Darling Lu Shao MIIT Key Laboratory of Critical materials Technology for New Energy Conversion and Storage State Key Laboratory of Urban Water Resource and EnvironmentSchool of Chemistry and Chemical EngineeringHarbin Institute of TechnologyHarbin 150001China Chemical Sciences and Engineering Division Argonne National LaboratoryLemont IL 60439USA Applied materials Division Argonne National LaboratoryLemont IL 60439USA Advanced materials for Energy-water Systems Energy Frontier Research Center Argonne National LaboratoryLemont IL 60439USA Pritzker School of Molecular Engineering University of ChicagoChicago IL 60637USA Department of Physics University of Ilinois at ChicagoChicago IL 60607USA School of Environment Harbin Institute of TechnologyHarbin 150090China wa School of Mines:Minerals Energy and Chemical EngineeringCurtin UniversityPerth WA 6845Australia

Atomic layer deposition(ALD)offers unique capabilities to fabricate atomically engineered porous materials with precise pore tuning and multi-functionalization for diverse applications like advanced membrane separations towards sustainable energy-water ***,current ALD technique is inhibited on most non-polar polymeric membranes due to lack of accessible nucleation ***,we report a facile method to efficiently promote ALD coating on hydrophobic surface of polymeric membranes via novel protein activation/*** a proof of concept,TiO2 ALD-coated membranes activated by bovine serum albumin exhibit remarkable superhydrophilicity,ultralow underwater crude oil adhesion,and robust tolerance to rigorous environments including acid,alkali,saline,and *** importantly,excellent cyclable crude oil-in-water emulsion separation performance can be *** mechanism for activation/sensitization is rooted in reactivity for a particular set of amino ***,the universality of protein-sensitized ALD is demonstrated using common egg white,promising numerous potential usages in biomedical engineering,environmental remediation,lowcarbon manufacturing,catalysis,and beyond.

关键词： Membrane separation Atomic layer deposition water treatment Antifouling

青藏高原尕斯库勒盐湖可培养嗜盐耐盐微生物的群落结构与分离方法对比研究

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海洋与湖沼 2024年第4期55卷 916-930页

作者：马欣马想蓉朱德锐李轩领沈国平邢江娃青海大学医学院基础医学研究中心青海西宁810016

嗜盐耐盐微生物在生物医药、生物酶等领域有着重要的应用潜力。为更好地利用青藏高原的盐湖微生物资源,以青藏高原尕斯库勒盐湖为研究对象,借助Illumina MiSeq测序平台分析盐湖微生物的群落组成,并采用寡营养培养、富集培养和梯度稀释... 详细信息

嗜盐耐盐微生物在生物医药、生物酶等领域有着重要的应用潜力。为更好地利用青藏高原的盐湖微生物资源,以青藏高原尕斯库勒盐湖为研究对象,借助Illumina MiSeq测序平台分析盐湖微生物的群落组成,并采用寡营养培养、富集培养和梯度稀释等方法对嗜盐耐盐微生物进行大规模分离与培养。结果表明,高通量测序获得明确分类地位的细菌有30门50纲81目162科417属,古菌6门7纲9目15科39属。细菌优势属类群以矿生菌属(Fodinibius)和纤维单胞菌属(Gracilimonas)为主。古菌优势属类群以盐红菌属(Halorubrum)为主;纯培养法共获得502株嗜盐耐盐微生物,其中嗜盐耐盐细菌472株,分布在4门5纲7目9科13属,优势属为芽孢杆菌属(Bacillus)和盐单胞菌属(Halomonas),其中巴纽尔斯菌(Balneolota)门Fodinibius属可培养细菌是首次从盐湖中分离得到;嗜盐古菌30株,分布在1门1纲1目3科5属,优势属为Halorubrum。对比不同培养条件下的分离结果发现,1/2 TSA培养基和1/2 RCA培养基更有利于嗜盐耐盐细菌的分离,最佳富集天数为7天和30天,稀释梯度10-1和10-2可与未稀释样本互为补充来增加可培养嗜盐耐盐细菌的多样性;1/102216E培养基更适合于古菌的分离,最佳富集天数为7天和14天,且样本不稀释分离效果更好。研究结果可为青藏高原盐湖可培养微生物的分离方案优化提供理论依据和数据支撑。

关键词：尕斯库勒盐湖嗜盐耐盐微生物分离培养生物多样性高盐环境

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An Eight Component Integrable Hamiltonian Hierarchy from a Reduced Seventh-Order matrix Spectral Problem

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Journal of Applied mathematics and Physics 2024年第6期12卷 2102-2111页

作者： Savitha Muthanna Wen-Xiu ma Department of Applied mathematics University of Washington Seattle WA USA Department of mathematics University of California Los Angeles Los Angeles CA USA Tektronix Inc. Beaverton Oregon USA Department of mathematics Zhejiang Normal University Jinhua China Department of mathematics King Abdulaziz University Jeddah Saudi Arabia Department of mathematics and Statistics University of South Florida Tampa FL USA material Science Innovation and Modelling North-West University Mafikeng Campus Mmabatho South Africa

We present an eight component integrable Hamiltonian hierarchy, based on a reduced seventh order matrix spectral problem, with the aim of aiding the study and classification of multicomponent integrable models and their underlying mathematical structures. The zero-curvature formulation is the tool to construct a recursion operator from the spatial matrix problem. The second and third set of integrable equations present integrable nonlinear Schrödinger and modified Korteweg-de Vries type equations, respectively. The trace identity is used to construct Hamiltonian structures, and the first three Hamiltonian functionals so generated are computed.

关键词： matrix Spectral Problem Zero Curvature Equation Lax Pair Integrable Hierarchy NLS Equations mKdV Equations Hamiltonian Structure Lie Bracke

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搜索服务响应时间异常诊断

计算机研究与发展 2024年第6期61卷 1573-1584页

作者：夏思博马明华金鹏翔崔丽月张圣林金娃孙永谦裴丹南开大学软件学院天津300457 先进计算与关键软件(信创)海河实验室天津300450 清华大学计算机科学与技术系北京100084

较低的网络服务响应时间对提升用户体验至关重要.以搜索引擎这一典型的网络服务场景为例,服务提供商应确保网络服务(搜索)响应时间在1 s以内.在实践中,服务响应时间会受到用户浏览器、运营商、页面加载方式等诸多服务属性的影响.为了进... 详细信息

较低的网络服务响应时间对提升用户体验至关重要.以搜索引擎这一典型的网络服务场景为例,服务提供商应确保网络服务(搜索)响应时间在1 s以内.在实践中,服务响应时间会受到用户浏览器、运营商、页面加载方式等诸多服务属性的影响.为了进行针对性的优化,服务提供商需要找出使服务响应时间过长的规则,即一些属性的组合.然而现有研究工作遇到了3方面挑战:1)搜索日志数据量大;2)搜索日志数据分布不平衡;3)要求泛化度高的规则.因此设计了Miner(multi-dimensional extraction of rules),一种新型服务响应时间异常诊断框架.Miner使用自步采样机制应对第1个挑战和第2个挑战.针对第3个挑战,Miner使用Corels算法挖掘出泛化率高且召回率高的规则.使用2家国内顶级搜索引擎服务提供商的响应时间日志数据评估了Miner性能,结果显示Miner的泛化率和召回率均高于现有方法,并证明了Miner挖掘出的规则可被运维人员采纳并做针对性的优化.

关键词：网络服务质量自步采样搜索引擎搜索响应时间数据挖掘

刺玫根多糖对环磷酰胺诱导的免疫抑制小鼠的免疫调节作用

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天然产物研究与开发 2024年第2期36卷 196-205,292页

作者：查苏娜苏日娜齐和日玛彭斯格热希萨仁高娃内蒙古医科大学呼和浩特010110

采用水提醇沉法提取刺玫根粗多糖,以Sevage法和TCA法脱蛋白,进一步分级醇沉法分离纯化得到较纯的60%醇沉物(RDRP)进行结构鉴定和活性评价。苯酚浓硫酸法测定出刺玫根多糖RDRP总糖含量为94.1%;高效凝胶渗透色谱法测定表明主要含有重均分... 详细信息

采用水提醇沉法提取刺玫根粗多糖,以Sevage法和TCA法脱蛋白,进一步分级醇沉法分离纯化得到较纯的60%醇沉物(RDRP)进行结构鉴定和活性评价。苯酚浓硫酸法测定出刺玫根多糖RDRP总糖含量为94.1%;高效凝胶渗透色谱法测定表明主要含有重均分子量为13.1 kDa,相对分散度为1.2的多糖;通过高效离子色谱法和红外光谱测定,鉴定刺玫根多糖为由葡萄糖、阿拉伯糖、半乳糖、甘露糖、木糖等5种单糖组成的含有α-构型的中性吡喃多糖。环磷酰胺诱导的免疫抑制小鼠模型实验结果表明,与模型组相比,刺玫根多糖各剂量组小鼠的脾脏和胸腺指数明显升高(P<0.05,P<0.01);血细胞分析仪检测发现白细胞数、红细胞数、血小板显著升高(P<0.05);ELISA法检测血清中免疫相关蛋白IgG、IgM、IgA以及细胞因子TNF-α、IFN-γ、IL-6与IL-2的分泌水平,结果显示,与模型组比较,刺玫根多糖组显著上调小鼠血清中免疫球蛋白IgA、IgG、IgM浓度水平(P<0.01)及IL-2、IFN-γ、TNF-α、IL-6的含量(P<0.01);Western blot法测定各组TLR4、MyD88、TRAF-6和NF-κB p65蛋白的表达水平,发现刺玫根多糖各剂量组脾脏中TLR4、TRAF-6蛋白极显著上升(P<0.01),高剂量组的MyD88蛋白极显著上升(P<0.01),其他组呈现上升趋势;刺玫根多糖各剂量组NF-κB p65蛋白均有上升趋势,因此其免疫调节作用机制可能与TLR/NF-κB通道有关。本研究初步阐明刺玫根多糖的基本结构特征和其免疫调节作用及其机制,为刺玫根多糖的开发应用提供一定价值的实验依据。

关键词：刺玫根多糖结构鉴定免疫调节 TLR/NF-κB

Virus-Host Interactions Drive Contrasting Bacterial Diel Dynamics in the Ocean

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Research 2024年第2期6卷 389-399页

作者： Xiaowei Chen Chen Hu Wei Wei Yunlan Yang markus G.Weinbauer Hongbo Li Shiying Ren Ruije ma YVibin Huang Tingwei Luo Nianzhi Jiao Rui Zhang State Key Laboratory of marine Environmental Science College of Ocean and Earth SciencesFujian Key Laboratory of Marine Carbon SequestrationXiamen UniversityXiamen 361102PR China College of the Environment and Ecology Xiamen UniversityXiamen 361102PR China Research Center for Environmental Ecology and Engineering School of Environmental Ecology and Biological EngineeringWuhan Institute of TechnologyWuhan 430205PR China Sorbonne Universités UPMCUniversitéParis 06CNRSLaboratoire d'Oceanographie de Villefranche(LOV)Villefranche-sur-Mer 06230France National marine Environmental Monitoring Center Ministry of Ecological EnvironmentDalian 116023PR China Department of Ocean Sciences University of CaliforniaSanta CruzCAUSA NOAA/OAR Pacific marine Environmental Laboratory SeattleWAUSA Institute for Advanced Study Shenzhen UniversityShenzhen 518055PR China

marine organisms perform a sea of diel *** diel dynamics have been shown to be driven by light,energy resources,circadian rhythms,and the coordinated coupling of photoautotrophs and heterotrophic ***,we explore the diel fluctuation of viral production and decay and their impact on the total and active bacterial community in the coastal and open seawaters of the South China *** results showed that the night-production diel pattern of lytic viral production was concurrent with the lower viral decay at night,contributing to the accumulation of the viral population size during the night for surface *** diel variations in bacterial activity,community composition,and diversity were found highly affected by viral *** was revealed by the finding that bacterial community diversity was positively correlated to lytic viral production in the euphotic zone of the open ocean but was negatively related to lysogenic viral production in the coastal *** distinct but contrasting correlations suggest that viral life strategies can not only contribute to diversifying bacterial community but also potentially piggyback their host to dominate bacterial community,suggesting the tightly synchronized depth-dependent and habitat-specific diel patterns of virus-host *** further implies that viruses serve as an ecologically important driver of bacterial diel dynamics across the ocean,highlighting the viral roles in bacterial ecological and biogeochemical processes in the ocean.

关键词： coastal ocean marine