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Antifungal Activity of Compounds Isolated from Bamboo Vermicompost against <i>Rhizoctonia solani</i>AG1-IB

Advances in Microbiology 2019年第12期9卷 957-970页

作者： Xiaodong You Daigo Wakana Kazuki Ishikawa Tomoo Hosoe motoaki tojo Graduate School of Life and Environmental Sciences Osaka Prefecture University Sakai Osaka Japan Department of Organic Chemistry Hoshi University Shinagawa Tokyo Japan Research Institute of Pharmaceutical Sciences Musashino University Nishitokyo-Shi Tokyo Japan

Vermicompost has become a promising alternative to chemical pesticide because of its disease suppression effect during these decades. However, the mechanisms by which vermicompost suppress plant disease are not well-understood. Antifungal compounds, which are released by beneficial organisms in the vermicompost, may play an important role in inhibiting plant pathogens;however, these mechanisms have not been widely examined. In this study, Sterilized Water Extracts of bamboo vermicompost (SWE), using a 0.22-μm cellulose acetate filter, significantly (P < 0.05) inhibited the mycelium growth of Rhizoctonia solani AG1-IB on a Potato Dextrose Agar (PDA) plate. This suggests that antifungal compounds are present in bamboo vermicompost. The ethanol acetate (EtOAc) crude extracts of bamboo vermicompost showing antifungal activity were further separated. Two compounds were isolated from the EtOAc fraction of bamboo vermicompost and characterized as ergosterol peroxide (1) and (22E, 24R)-5α,8α-epidioxyergosta-6,9(11),22-trien-3β-ol (2). Their chemical structures and mass spectra were determined by nuclear magnetic resonance and mass spectrometry analyses. Ergosterol peroxide tested at 150, 300, 600, and 900 μg showed 13%, 22%, 34%, and 53% mycelial growth inhibition against R. solani AG1-IB, respectively. Because EtOAc crude extracts of the initial substrate materials of bamboo vermicompost did not inhibit mycelium growth of R. solani AG1-IB, antifungal compounds in the vermicompost may be released by microbes but not from the original substrates during vermicomposting.

关键词： Antifungal Compound Ergosterol Peroxide Vermicompost Rhizoctonia solani AG1-IB

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Channel Attention GAN-Based Synthetic Weed Generation for Precise Weed Identification

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Plant Phenomics 2024年第2期6卷 257-268页

作者： Tang Li motoaki Asai Yoichiro Kato Yuya Fukano Wei Guo Graduate School of Agricultural and Life Sciences The University of TokyoTokyo 188-0002Japan Institute for Plant Protection National Agriculture and Food Research OrganizationFukushima 960-2156Japan Graduate School of Horticulture Chiba UniversityChiba 271-0092Japan

Weed is a major biological factor causing declines in crop ***,widespread herbicide application and indiscriminate weeding with soil disturbance are of great concern because of their environmental ***-specific weed management(SSWM)refers to a weed management strategy for digital agriculture that results in low energy *** learning is crucial for developing SSWM,as it distinguishes crops from weeds and identifies weed ***,this technique requires substantial annotated data,which necessitates expertise in weed science and agronomy.

关键词： identification attention generation channel gan-based precise synthetic weed

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Analysis of the Abnormal Segregation of Pathogenicity in Magnaporthe grisea by Using a Genetic Cross of Oryza and Eleusine Isolates

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Agricultural Sciences in China 2010年第3期9卷 383-391页

作者： YIN Liang-fen LUO Chao-xi Kusaba motoaki Yaegashi Hiroshi Department of Plant Pathology College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 P.R.China Key Lab of Crop Disease Monitoring ＆ Safety Control in Hubei Province Huazhong Agricultural University Wuhan 430070 P.R.China Faculty of Agriculture Saga University Saga 840-8502 Japan

A genetic cross between Oryza isolate Y93-164a-1 and Eleusine isolate SA98-4 was established, and the pathogenicity of 151 F1 progeny isolates was investigated on both host plants rice and finger millet. Results showed that the segregation of pathogenicity in this genetic cross was abnormal, i.e., most of the progeny isolates were nonpathogenic on both host plants. However, no abnormal segregation was observed when middle repetitive sequence MGR586 and 31 single-copy RFLP markers from all of the chromosomes were genetically analyzed. At the same time, comparison of the chromosomal organization among two pairs of parental isolates did not find any genomic abnormity. These results suggested that the ＂abnormal＂ inheritance of pathogenicity in this cross was most likely due to the reassortment of numerous host species specificity genes but not the biased segregation of the host species specificity genes. The host species specificities in M. grisea were likely to be multigenically controlled, at least in the genetic cross involving rice pathogen and the grasses pathogen other than rice.

关键词： Magnaporthe grisea rice blast fungus host species specificity pathogenicity contour-clamped homogeneous electric field (CHEF) electrophoresis Southern hybridization

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Prediction of flare-ups of ulcerative colitis using quantitative immunochemical fecal occult blood test

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World Journal of Gastroenterology 2010年第9期16卷 1110-1114页

作者： motoaki Kuriyama Jun Kato Koji Takemoto Sakiko Hiraoka Hiroyuki Okada Kazuhide Yamamoto Department of Gastroenterology and Hepatology Okayama University Graduate School of MedicineDentistryand Pharmaceutical Sciences

AIM:To examine the feasibility of predicting the flareup of ulcerative colitis (UC) before symptoms emerge using the immunochemical fecal occult blood test (IFOBT).METHODS:We prospectively measured fecal hemoglobin concentrations in 78 UC patients using the I-FOBT every 1 or 2 ***:During a 20 mo-period,823 fecal samples from 78 patients were *** median concentration of fecal hemoglobin was 41 ng/mL (range:0-392 500 ng/mL).There were three types of patients with regard to the correlation between I-FOBT and patient symptoms;the synchronous transition type with symptoms (44 patients),the unrelated type withsymptoms (19 patients),and the flare-up predictive type (15 patients).In patients with the flare-up predictive type,the values of I-FOBT were generally low during the study period with stable *** to four weeks before the flare-up of symptoms,the I-FOBT values were ***,in these patients,I-FOBT could predict the flare-up before symptoms ***:Flare-up could be predicted by I-FOBT in approximately 20% of UC *** results warrant periodical I-FOBT in UC patients.

关键词： Flare-ups Immunochemical fecal occult blood test Inflammatory bowel disease Prediction Ulcerative colitis

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Fluorescent SiC and its application to white light-emitting diodes

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Journal of Semiconductors 2011年第1期32卷 24-26页

作者： Satoshi Kamiyama motoaki Iwaya Tetsuya Takeuchi Isamu Akasaki Mikael Syvjrvi Rositza Yakimova Department of Materials Science and Engineering Meijo University Department of Physics Chemistry and Biology Linkping University

Fluorescent-SiC （f-SiC）, which contains donor and acceptor impurities with optimum concentrations, has high conversion efficiency from NUV to visible light caused by donor-acceptor-pair （DAP） recombination. This material can be used as a substrate for a near UVlight-emitting diode （LED） stack, and leads to monolithic white LED device with suitable spectral property for general lighting applications. In this paper, we describe basic technologies of the white LED, such as optical properties of f-SiC substrate, and epitaxial growth of NUV stack on the f-SiC substrate.

关键词： white LED phosphor SiC donor-acceptor-pair GaN general lighting

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Pseudo-Meigs' syndrome secondary to metachronous ovarian metastases from transverse colon cancer

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World Journal of Gastroenterology 2016年第18期22卷 4604-4609页

作者： Kennoki Kyo Atsushi Maema motoaki Shirakawa Toshio Nakamura Kenji Koda Hidetaro Yokoyama Department of Surgery Fujieda Municipal General Hospital Department of Pathology Fujieda Municipal General Hospital

Pseudo-Meigs’ syndrome associated with colorectal cancer is extremely rare. We report here a case of pseudo-Meigs’ syndrome secondary to metachronous ovarian metastases from colon cancer. A 65-year-old female with a history of surgery for transverse colon cancer and peritoneal dissemination suffered from metachronous ovarian metastases during treatment with systemic chemotherapy. At first, neither ascites nor pleural effusion was observed, but she later complained of progressive abdominal distention and dyspnea caused by rapidly increasing ascites and pleural effusion and rapidly enlarging ovarian metastases. Abdominocenteses were repeated, and cytological examinations of the fluids were all negative for malignant cells. We suspected pseudo-Meigs’ syndrome, and bilateral oophorectomies were performed after thorough informed consent. The patient’s postoperative condition improved rapidly after surgery. We conclude that pseudo-Meigs’ syndrome should be included in the differential diagnosis of massive or rapidly increasing ascites and pleural effusion associated with large or rapidly enlarging ovarian tumors.

关键词： Pseudo-Meigs’ syndrome Colon cancer Ascites Pleural effusion Ovarian metastasis

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mpact of antioxidants on seminal vesicles function ind fertilizing potential in diabetic rats

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Asian Journal of Andrology 2017年第6期19卷 639-646页

作者： Panagiota Tsounapi Masashi Honda Fotios Dimitriadis Bunya Kawamoto Katsuya Hikita Kuniyasu Muraoka motoaki Saito Nikolaos Sofikitis Atsushi Takenaka Division of Urology Department of Surgery Tottori University School of Medicine 36-1 Nishimachi Yonago 683-8503 Japan Department of Urology University of Ioannina School of Medicine P.O. Box 1186 45110 Ioannina Greece Department of Pharmacology Kochi University Kochi Medical School Nankoku 783-8505 Japan.

Diabetes mellitus significantly affects the male reproduction and sexual function. In the present study, we investigated the diabetes-induced dysfunction of seminal vesicles （SVs） in the diabetes-rat model and the role of antioxidants. Streptozotocin-induced diabetes after 4 weeks caused smaller size of the organs, hypercontractility, histological abnormalities, increased concentrations of malondialdehyde in the serum and tissue, overexpression of oxidative stress markers, and cleaved caspase-3 as identified by immunohistochemistry in the SVs. In addition, diabetes resulted in deceased levels of serum testosterone and no newborns after the mating studies. Antioxidants significantly normalized all the above parameters, except for the severely decreased serum testosterone levels and the negative outcome of the mating studies. The present study gives evidence for the important role of diabetes-induced oxidative stress in the function and structure of these androgen-dependent organs. Antioxidants may be a promising supplementary therapy for diabetic male patients to alleviate ejaculatory disorders but alone is not efficient treatment for the mitigation of infertility.

关键词： diabetes mellitus male infertility oxidative stress seminal vesicles seminiferous epithelium smooth muscle cellcontractions

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Titanium Culture Vessel Presenting Temperature Gradation for the Thermotolerance Estimation of Cells

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Cyborg and Bionic Systems 2023年第1期4卷 166-177页

作者： Chikahiro Imashiro Yangyan Jin motoaki Hayama Takahiro GYamada Akira Funahashi Katsuhisa Sakaguchi Shinjiro Umezu Jun Komotori Graduate School of Engineering The University of TokyoTokyo 113-0033Japan Department of Mechanical Engineering Keio UniversityYokohamaKanagawa 223-0061Japan School of Integrated Design Engineering Graduate School of Science and TechnologyKeio UniversityYokohamaKanagawa 223-0061Japan Department of Biosciences and Informatics Keio UniversityYokohamaKanagawa 223-0061Japan Department of Integrative Bioscience and Biomedical Engineering Graduate School of Advanced Science and EngineeringWaseda UniversityTWInsTokyo 162-8480Japan Department of Modern Mechanical Engineering Waseda UniversityTokyo 169-8555Japan

Hyperthermia can be induced to exploit the thermal intolerance of cancer cells,which is worse than that of normal cells,as a potential noninvasive cancer *** develop an effective hyperthermia treatment,thermal cytotoxicity of cells should be comprehensively ***,to conduct such investigations,the culture temperature must be accurately *** previously reported a culture system in which the culture temperature could be accurately regulated by employing metallic culture ***,appropriate temperature conditions for hyperthermia depend on the cell ***,several experiments need to be conducted,which is a bottleneck of inducing ***,we developed a cell culture system with temperature gradation on a metallic culture *** Cancer Foundation-7 cells and normal human dermal fibroblasts were used as cancer and normal cell models,*** cells showed stronger thermal tolerance;this was because the novel system immediately exhibited a temperature ***,the developed culture system can be used to investigate the optimum thermal conditions for effective hyperthermia ***,as the reactions of cultured cells can be effectively assessed with the present results,further research involving the thermal stimulation of cells is possible.

关键词： stimulation culture Titanium

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Essentials of Creativity

Essentials of Creativity

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全国第六届心理学学术会议

作者： Hiroshi motoaki Japanese Psychological Association Japan

Essentials of creativity signify among other things representation of thinking and ideas through verbal expressions mainly listening and talking, implying that the more creative person is able to express more than tho...

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Prostaglandin E2 induces cardiomyocyte hypertrophy via β-catenin dependent pathway

Prostaglandin E2 induces cardiomyocyte hypertrophy via β-ca...

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第十三次全国心血管病学术会议

作者： motoaki Sano Keiichi Fukuda Department of Cardiology Keio UniversityJapan

Objective The purpose of this study was to investigate whether prostaglandin E2(PGE2) induces beta-catenin activation in neonatal rat ventricular cardiomyocytes and if so to determine the possible role of this activation in PGE2-induced hypertrophic *** Beta-catenin activation activation was determined by Western *** levels of fetal genes beta-myosin heavy chain(beta-MHC) activation and atrial narriuretic peptide(ANP) were quantified by real-time *** surface was quantified by microscopic *** We found that PGE2(100 nM) induces time-dependent

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