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Effect of partial depletion of CD25+ T cells on neurological deficit and tissue damage in acute cerebral ischemia rat models

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Journal of Acute Disease 2018年第6期7卷 247-253页

作者： Ana L Rodríguez-Perea Johanna Gutierrez-Vargas Mauricio Rojas Gloria P cardona-Gómez Paula A Velilla Grupo Inmunovirologia Facultad de MedicinaUniversidad de Antioquia UdeACalle70No.52-21MedellínColombia Bacterias&Cáncer Facultad de MedicinaUniversidad de Antioquia UdeACalle70No.52-21MedellínColombia Facultad de Ciencias de la Salud Corporación Universitaria RemingtonUniremingtonCalle51No.51-27MedellínColombia Grupo de Inmunología Celular e Inmunogenética(GICIG).Instituto de Investigaciones Médicas Facultad de MedicinaUniversidad de Antioquia UdeACalle70No52-21MedellínColombia Unidad de Citometría Facultad de MedicinaSede de Investigación UniversitariaUniversidad de Antioquia UdeACalle70No52-21MedellínColombia Grupo de Neurociencias de Antioquia Facultad de MedicinaSede de Investigación UniversitariaUniversidad de Antioquia UdeACalle70No52-21MedellínColombia

Objective: To evaluate the role of regulatory T cells (Tregs) at late stages of stroke. Methods:Anti-CD25 antibody (or PBS as a control) was injected to reduce the pool of Tregs in Wistar rats;then, ischemia was induced transiently by middle cerebral artery occlusion during 60 min and reperfusion was allowed for 7 d. Then, Treg frequency was analyzed in peripheral blood, spleen and lymph nodes. Neurological score (0-6) and infarct volume were also determined. Results: Nine days after injection, the CD4+CD25+ T cells were reduced by 70.4%, 44.8% and 57.9% in peripheral blood, spleen and lymph nodes, respectively compared to PBS-treated rats. In contrast, the reduction of CD4+FOXP3+ T cells was lower in the same compartments (38.6%, 12.5%, and 29.5%, respectively). The strongest reduction of CD25+CD4+ T cells was observed in those FOXP3-negative cells in blood, spleen and lymph nodes (77.8%, 52.8%, and 60.7%, respectively), most likely corresponding to activated T cells. Anti-CD25-treated transient middle cerebral artery occlusion rats had a lower neurological deficit and did not develop tissue damage compared with PBS-treated animals. Conclusions: These findings suggest that treatment with anti-CD25 in our model preferentially reduce the T cell population with an activated phenotype, rather than the Treg population, leading to neuroprotection by suppressing the pathogenic response of effector T cells.

关键词： Transitory middle cerebral artery occlusion Rat Anti-CD25 Regulatory T cells Ischemia Activated T-lymphocytes

A Neuro-Fuzzy System for the Post-Earthquake Damage Assessment of Buildings

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A Neuro-Fuzzy System for the Post-Earthquake Damage Assessme...

Enterovirus 71 blocks interferon-activated JAK/STAT Signal Transduction by inducing Karyopherin-α1 Degradation

The Sixth World Congress on Computational Mechanics in Conjunction with the Second Asian-Pacific Congress on Computational Mechanics

作者： Martha L.Carreno Omar D.cardona Alex H.Barbat Department of Structural Mechanics Technical University of Catalonia Jordi Girona 1 -3 Modulo C1 Barcelona 08034 Spain Department of Civil Engineering University of Los Andes 092858 Bogota D.C. Colombia Department of Structural Mechanics Technical University of Catalonia Jordi Girona 1 -3 Modulo C1 Barcelona 08034 Spain

After a strong earthquake the state of the affected buildings should be assessed in order to define their habitability and reparability. Due to the extension of damage in the str uck area, it is not possible to make the evaluations of all the building only by expert engineers. Many inspectors without expertise and knowledge must be involved due to the need of massive survey and the necessity to have the diagnosis as soon as possible to aid the people affected. As a consequence, it is common the tendency of non-expert inspectors to aggravate or to underestimate the real level of damage. This paper develops the algorithmic basis of an integrated tool of computational intelligence tech nique to assist non-experts in the quick building damage diagnosis and occupancy decision -making. A hybrid neuro-fuzzy system is proposed, based on a special three -layer feed-forward artificial neural network and fuzzy rule bases [1-2]. The inputs of the system are fuzzy sets, taking into account that the damage levels of the structural components are linguistic defined by means of qualifications such as light, minor, moderate, average, severe, etc., which are very appropriate to handle subjective and incomplete information. A Kohonen unsupervised learning algorithm is used, as a first module, to assess the structural damage degree. The weights for each appraisal are obtained by the T -conorm of the triangular membership functions, which represent the input d amage level qualifications, and by fitting to a sigmoidal damage function of reference. For the training of the artificial neural network, damage evaluations made after the Quindio's earthquake in Colombia (1999) were used. A fuzzy rule bases module is then used for the habitability and reparability decision -making. It takes into account: 1) the building safety, obtained from the damage evaluation in structural and architectural components, 2) the site seismic effects and 3) the pre -existing conditions that can make the building

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Enterovirus 71 blocks interferon-activated JAK/STAT Signal T...

Metamorphic gradient modification in the Early Cretaceous Northern Andes subduction zone:A record from thermally overprinted high-pressure rocks

第十三届江浙沪儿科学术会议暨2016年浙江省医学会儿科学学术年会

作者： Chunyang Wang Lianfu Ji Yu Jin Carol J.cardona Zheng Xing Medical School Jiangsu Provincial Key Laboratory of Medicineand the State Key Laboratory of Pharmaceutical TechnologyNanjing University Nanjing Children's Hospital College of Veterinary Medicine University of Minnesota at Twin Cities

Enterovirus 71(EV71) has been emerged as a clinically important virus since poliovrirus eradiation,and no effective antiviral drugs are available for *** I interferons(IFN-α/β) serves as the first defense of virus infection and have been used as antiviral drugs successfully for *** clinically treatment with Type Ⅰinterferons did not protect the patients from EV71 *** we confirmed that in vitro study,pretreated with IFN-β did not protect the cells from cytopathic effect induced by EV71,or inhibit the replication of *** reports were conflicted about the mechanism of EV71 antagonizing the interferon *** study demonstrated that EV71 infection blocked interferon signal pathway through inhibiting the nuclear translocation but not inhibiting the phosphorylation of *** found that EV71 infection did not change die phosphorylation status of STATs as well as the protein level of unphosphorylated proteins,while IFN-β-induced the translocation of phosphorylated STAT1/2(p-STAT1/2) was significantly blocked by EV71 *** study showed that EV71 infection reduced STAT1/KPNA1 complex after interferon stimulation and reducing the protein level of KPNAl,the nuclear localization signal receptor for tyrosine-phosphorylated *** specific inhibitors,we observed that EV71 infection induced degration of endonous KPNA1 in a caspase-3-dependent ***,we examined die activity of the 2 A and 3 C proteases on the activity of IFN-β.The results showed that both the two proteases had no inhibition effect on die activation of ISRE or the production of ISGs induced by IFN-β and didn’t influence the protein level of *** together,EV71 infection inhibited the interferon system by inducing the degration of KPNA1 by a caspase-3-dependent pathway.

关键词： Enterovirus 71 JAK STAT IFN-β

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Geoscience Frontiers 2022年第2期13卷 354-371页

作者： D.S.Avellaneda-Jiménez A.cardona V.Valencia S.León I.F.Blanco-Quintero Grupo de Investigación en Geología y Geofisica(EGEO) Universidad Nacional de ColombiaMedellínColombia Departamento de Materiales y Minerales Universidad Nacional de ColombiaMedellínColombia Departamento de Procesos y Energía Universidad Nacional de ColombiaMedellínColombia School of the Environment Washington State UniversityPullmanUSA Smithsonian Tropical Research Institute BalboaAnconPanama Departamento de Ciencias de la Tierraydel Medio Ambiente Universidad de AlicanteSpain

New field observations and petrological data from Early Cretaceous metamorphic rocks in the Central Cordillera of the Colombian Andes allowed the recognition of thermally overprinted high-pressure rocks derived from oceanic crust *** obtained metamorphic path suggests that the rocks evolved from blueschist to eclogite facies towards upper amphibolite to high-pressure granulite facies transitional *** facies conditions,better recorded in mafic protoliths,are revealed by relic lawsonite and phengite,bleb-to worm-like diopside-albite symplectites,as well as garnet core *** amphibolite to high pressure granulite facies overprinting is supported by coarse-grained brown-colored Ti-rich amphibole,augite,and oligoclase recrystallization,as well as the record of partial melting *** equilibria and pressure-temperature(P-T)path modeling suggest initial high-pressure metamorphic conditions M1 yielding 18.2-24.5 kbar and 465-580℃,followed by upper amphibolite to high pressure granulite facies overprinting stage M2 yielding 6.5-14.2 kbar and 580-720℃.Retrograde conditions M3 obtained through chlorite thermometry yield temperatures ranging around 286-400℃at pressures below 6.5-11 *** obtained clockwise P-T path,the garnet zonation pattern revealing a decrease in X_(grs)/X_(prp)related to Mg#increment from core to rim,the presence of partial melting veins,as well as regional constraints,document the modification of the thermal structure of the active subduction zone in Northern Andes during the Early *** increment of the metamorphic gradient within the subduction interface is associated with slab roll-back geodynamics where hot mantle inflow was *** scenario is also argued by the reported trench-ward magmatic arc migration and multiple extensional basin formation during this *** presented example constitutes the first report of Cretaceous roll-back-related metamorphism in the Caribbean and Ande

关键词： Slab roll-back Mineral chemistry Phase equilibria P-T path modeling Roll-back metamorphism Thermally overprinted high-pressure rocks

Lanthanide molecular nanomagnets as probabilistic bits

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npj Computational Materials 2023年第1期9卷 322-330页

作者： Gerliz M.Gutiérrez-Finol Silvia Giménez-Santamarina Ziqi Hu Lorena E.Rosaleny Salvador cardona-Serra Alejandro Gaita-Ariño Instituto de Ciencia Molecular(ICMol) Universitat de ValènciaPaternaSpain Department of Materials Science and Engineering CAS Key Laboratory of Materials for Energy ConversionAnhui Laboratory of Advanced Photon Science and TechnologyUniversity of Science and Technology of China230026 HefeiPeople’s Republic of China

Over the decades,the spin dynamics of a large set of lanthanide complexes have been ***-based molecular nanomagnets are bistable spin systems,generally conceptualised as classical bits,but many lanthanide complexes have also been presented as candidate quantum bits(qubits).Here,we offer a third alternative and model them as probabilistic bits(p-bits),where their stochastic behaviour constitutes a computational resource instead of a *** an ad-hoc modelling tool for molecular spin p-bits and molecular nanomagnets,we simulate a minimal p-bit network under realistic ***,we go back to a recently published dataset and screen the best lanthanide complexes for p-bit behaviour,lay out the performance of the different lanthanide ions and chemical families and offer some chemical design considerations.

关键词： lanthanide magnets Lanthanide

Optimization and Validation of a Method for Heavy Metals Quantification in Soil Samples by Inductively Coupled Plasma Sector Field Mass Spectrometry(ICP-SFMS)

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American Journal of Analytical Chemistry 2013年第10期4卷 9-15页

作者： Héctor Hernández-Mendoza Marcos Mejuto Ana Isabel cardona Avelino García-Alvarez Rocio Millán Abel Yllera Instituto Nacional de Investigaciones Nucleares Carretera México-TolucaLa Marquesa OcoyoacacMéxico Departamento de Medio Ambiente Centro de Investigaciones EnergéticasMedioambientales y Tecnológicas(CIEMAT)AvdaMadridEspana

In this work, a method for quantification of heavy metals Inductively Coupled Plasma Sector Field Mass Spectrometry (ICP-SFMS) in soil samples of El Bierzo district (Spain) has been optimized and validated. Optimization was carried out for elements: Cr, Mn, Co, Ni, Cu, Zn, Se, As, Cd, Hg, Pb and U. Validation of the method was performed with Certified and Standard Reference Materials (CRMs and SRMs);SRM2709, CRM020-051 and CRM050-051. Results obtained under optimized conditions can be summarized as follows: a) the Limits of Detection (LODs) were in the order of sub fg·g-1 for Cr, Mn, Cu, Co, As, Cd, Hg, Pb and U, and few fg·g-1for Ni, Zn and Se;b) precision measurement, in terms of relative standard deviation (RSD), was been below 5%;c) the average recovery of CRM was between 81.3% and 98%. In conclusion, the method offers several advantages: fast, good accuracy, very low values of Limits of Quantification (LOQs) and high sensitivity on measurement of heavy metal.

关键词： Soil Heavy Metal ICP-SFMS

Adverse events in critical care: Search and active detection through the Trigger Tool

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World Journal of Critical Care Medicine 2018年第1期7卷 9-15页

作者： Francisco J Molina Paula T Rivera Alejandro cardona Diana C Restrepo Oralia Monroy Daniel Rodas Juan G Barrientos Clínica Universitaria Bolivariana School of Medicine Universidad Pontificia Bolivariana Faculty of Nursing Universidad de Caldas School of Medicine Universidad Pontificia Bolivariana Clínica Universitaria Bolivariana

AIM To investigate the incidence of disadvantageous events by using the Global Trigger Tool in an intensive care unit(ICU).METHODS A retrospective descriptive study was performed in a 12-bed university ICU in the city of Medellin, Colombia. Clinical charts of hospitalized patients were reviewed, between January 1 and December 31, 2016, with the following inclusion criteria: subjects aged over 18 years, with at least 24 h of hospitalization and who had a complete medical history that could be accessed. Interventions: Trained reviewers conducted a retros pective examination of medical charts searching for clue events that elicit investigation, in order to detect an unfavorable event. Measurements: Information was processed through SPSS softwareversion 21; for numerical variables, the mean was reported with standard deviation(SD). Percentages were calculated for qualitative variables. RESULTS Two hundred and forty-four triggers occurred, with 82.4% of subjects having presented with at least one and an average of 3.37 (SD 3.47). A total of 178 adverse events (AEs) took place in 48 individuals, with an incidence of 52.1%. On average, four events per patient were recorded, and for each unfortunate event, 1.98 triggers were presented. The most frequent displeasing issues were: pressure ulcers(17.6%), followed by complications or reactions to medical devices(4.3%), and lacerations or skin defects(3.7%); the least frequent was delayed diagnosis or treatment (0.56%). Thirty-eight point four percent of mishap events caused temporary damage that required intervention, and 48.9% of AEs were preventable. Comparison between AEs and admission diagnoses found that hypertension and sepsis were the only diagnoses that had statistical significance (P = 0.042 and 0.022, respectively).CONCLUSION Almost half of the unfavorable issues were classified as avoidable, which leaves a very wide field of work in terms of preventative activities.

关键词： Adverse events Critical care Trigger Tool Complications Security

Prevalence of and risk factors for erectile dysfunction in young nondiabetic obese men:results from a regional study

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Asian Journal of Andrology 2020年第4期22卷 372-378页

作者： María Molina-Vega Maite Asenjo-Plaza María JoséBanderas-Donaire María Dolores Hernández-Ollero Silvia Rodríguez-Moreno Juan Jálvarez-Millán Pablo Cabezas-Sanchez Fernando cardona-Díaz Juan Alcaide-Torres Lourdes Garrido-Sánchez Daniel Castellano-Castillo Francisco J Tinahones JoséC Fernández-García Department of Endocrinology and Nutrition Virgen de la Victoria University Hospital(IBIMA)Málaga UniversityMálaga 29010Spain Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición(CIBEROBN) Instituto de Salud Carlos IIIMadrid 28029Spain Cruz de Humilladero Primary Care Center Málaga 29010Spain Teatinos Primary Care Center Málaga 29010Spain Chemical Sanitary Consulting(CQS Lab) Madrid 28003Spain

Erectile dysfunction(ED),a condition closely related to cardiovascular morbidity and mortality,is frequently associated with *** this study,we aimed to determine the prevalence of ED and evaluate the associated risk factors in a cohort of 254 young(18–49 years)nondiabetic obese(body mass index[BMI]≥30 kg m−2)men from primary *** function(International Index of Erectile Function[IIEF-5]questionnaire),quality of life(Aging Males'Symptoms[AMS scale]),and body composition analysis(Tanita MC-180MA)were *** testosterone was determined using high-performance liquid chromatography–mass *** logistic regression analysis was used to study the factors associated with *** prevalence was 42.1%.Subjects with ED presented higher BMI,waist circumference,number of components of the metabolic syndrome,AMS score,insulin resistance,and a more unfavorable body composition than those without *** logistic regression analysis showed that a pathological AMS score(odds ratio[OR]:4.238,P40%of subjects.A pathological AMS score,the degree of obesity,and age were positively associated with ED,while elevated HDL-cholesterol levels were inversely associated with the odds of presenting *** prospective studies are needed to evaluate the long-term consequences of ED in this population.

关键词： Aging Males'Symptoms score erectile dysfunction International Index of Erectile Function-5 questionnaire obesity testosterone

Re-emerging threat of Trypanosoma cruzi vector transmission in El Salvador, update from 2018 to 2020

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Infectious Diseases of Poverty 2022年第4期11卷 101-102页

作者： Marvin Stanley Rodriguez Yuko Nitahara Michelle Cornejo Kevin Siliezar Rafael Grande Ana Gonzalez Kotaro Tasaki Yu Nakagama Yu Michimuko Yoko Onizuka Junko Nakajima-Shimada Jose Eduardo Romero Jose Ricardo Palacios Carmen Elena Arias Wilam Mejia Yasutoshi Kido Ricardo cardona Alvarenga Department of Virology and Parasitology Graduate School of MedicineOsaka Metropolitan University1-4-3 Asahi-machiAbeno-kuOsaka 545-8585 Japan 不详

Background:Since the late twentieth century,Chagas disease gained global attention to suppress the vector burden as a main control strategy in endemic *** Central America,multi-national initiative successfully achieved significant reduction in the estimated disease prevalence as well as elimination of the region's principal vector species at the time in *** the last decade has witnessed significant changes in ecosystem-such as urbanization and replacement of the main vector species-that can possibly affect the vector's habitation and residual transmission,the up-to-date vector burden in the region has not been evaluated thoroughly due to the cessation of active vector *** aim of this study was to update the risk of vector-borne Trypanosoma cruzi infection in El Salvador,the top Chagas disease-endemic country in Central ***:A nationwide vector survey was conducted in the domestic environment of El Salvador from September 2018 to November *** selection of the houses for inspection was based on expert purposeful *** for *** was examined by microscopic observation of the insects'feces,followed by a species confrmation using *** data were analyzed using R software version *** estimates with 95%confidence intervals were inferred using the Jeffrey's method provided under the epiR ***:A total of 1529 Triatoma dimidiata was captured from 107 houses(infestation rate,34.4%;107/311)in all the fourteen departments of the country visited within the period;prevalence of *** infection was as high as 10%(153/1529).In the country,domestic *** infestation was distributed ubiquitously,while *** infection rates varied across the *** out of fourteen departments showed higher infection rates than the average,suggesting sporadic high-risk areas in the ***:Our comprehensive study revealed substantial *** infection of *** across the country,in

关键词： Chagas disease Vector transmission Triatomine Trypanosoma cruzi Triatoma dimidiata