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Neural Regeneration Research 2025年第9期20卷 2581-2582页

作者： Marianne Bardy-Lagarde Narimène Asbelaoui abdel Mouman Ghoumari UMR1195 “Diseases and Hormones of the Nervous System”Inserm and University Paris-SaclayKremlin-BicêtreFrance

Multiple sclerosis(MS)is the most common chronic disease of the central nervous system(CNS)in young adults and represents the first cause of severe handicap,originally non-traumatic(Oh et al.,2018).MS is chara cterized by the infiltration of auto reactive lymphocytes specific to myelin through the blood-brain barrier,which results in the appearance of inflammatory demyelinating lesions caused by the death of the central nervous system myelinating cells,oligodendrocytes(Oh et al.,2018).There is a prevalence sexual with a ratio of three times more affected women than men.

关键词： myelin CXCR4

Detection of HER-2/neu Amplification on Fine Needle Aspirates of Breast Cancer Using Fluorescence in Situ Hybridization

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Journal of Cancer Therapy 2013年第7期4卷 41-48页

作者： Talaat Iman Sorour Amani abdel-hadi mona Clinical Pathology Alexandria Univer- sity Alexandria Egypt Departments of Pathology Faculty of Medicine Alexandria University Alexandria Egypt

The accuracy of diagnostic assays for HER-2/neu in breast cancer is extremely important as HER-2/neu status is essential in tailoring adjuvant and/or neoadjuvant treatment in every patient. FNAC is widely practiced in Egypt in preoperative diagnosis of breast cancer for its low cost and high diagnostic accuracy. Since the determination of HER-2/neu protein expression on cytological preparations was previously found to be unreliable for clinical use, we opted for the assessment of HER-2/neu status in fine needle aspirates using FISH. The main objective of this study was to evaluate the reliability of HER-2/neu status assessment by FISH on fine needle aspirates of breast cancers by comparing the results with IHC and FISH on FFPE tissue sections obtained from corresponding surgically resected specimens. Fine needle aspirates from 40 breast cancer patients with pathologically confirmed breast cancer were included in the study. They were submitted for HER-2/neu evaluation by FISH. After surgery, the corresponding FFPE sections were evaluated for HER-2/neu by FISH and by IHC. FNAs from 11 cases proved to be amplified by FISH, while 29 cases were not amplified. Apart from two cases that showed lack of signals, all specimens evaluated by FISH on the corresponding FFPE sections showed matched results. The Measurement of Agreement between FISH on FNAs and FISH on FFPE sections was 86.7%, while that between FISH on FNAs and IHC was 72.5%. The high concordance rate in the present study between FISH evaluation of HER-2/neu gene amplification on FNAC samples and their corresponding FFPE samples indicate that FISH may be a reliable technique for HER-2/neu assessment on FNAs. Furthermore, FISH on FNAs gave us better hybridization signals than their corresponding FFPE tissue sections. Finally, we also conclude that all score (2+) cases by IHC should be reevaluated by FISH which is crucial for the patient management.

关键词： Breast Cancer FISH on FNA Immunohistochemistry HER-2/neu

Differential expression of cell cycle regulators in HCV-infection and related hepatocellular carcinoma

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World Journal of Hepatology 2010年第1期2卷 32-41页

作者： Azza E El Bassiouny mona M Nosseir mona K Zoheiry Noha A Ameen Ahmed M abdel-hadi Ibrahim M Ibrahim Suher Zada abdel-Hakeem Saad El-Deen Nora E El-Bassiouni Department of Immunology Theodor Bilharz Research Institute Department of Pathology Central Laboratory Theodor Bilharz Research Institute Department of Hepato-Gastroenterology Theodor Bilharz Research Institute Biology Department American University Zoology Department Faculty of ScienceCairo University Department of Hematology Theodor Bilharz Research Institute

AIM:To investigate cell cycle proteins in chronic hepatitis C virus infection in order to analyze their role in the process of hepatocyte transformation and to characterize their prognostic properties. METHODS:Subjects of the current study included 50 cases of chronic hepatitis C(CHC) without cirrhosis,30 cases of CHC with liver cirrhosis(LC) ,and 30 cases of hepatitis C-related hepatocellular carcinoma(HCC) admitted to the Department of Hepato-Gastroenterology,Theodor Bilharz Research Institute(TBRI) ,Giza,*** wedge liver biopsies,taken during laparoscopic cholecystectomy,were also included as normal *** investigations including urine and stool analysis,liver function tests and prothrombin concentration;serologic markers for viral hepatitis and ultrasonography were done for all cases of the study together with immunohistochemical analysis using primary antibodies against Cyclin D1,Cyclin E,p21,p27 and Rb/p105 proteins. RESULTS:Normal wedge liver biopsies didn't express Cyclin E or Rb/p105 immunostaining but show positive staining for Cyclin D1,p21 and *** D1 expressed nuclear staining that was sequentially increased from CHC to LC(P<0.01) to HCC(P<0.001) cases;meanwhile,Cyclin E revealed nuclear positivity only in the case of HCCs patients that was directly correlated to Rb/p105 *** expression of p21 and p27 was significantly increased in CHC and LC cases compared to normal controls and HCCs with no significant difference between well-and poorlydifferentiated tumors.p21 showed only a nuclear pattern of staining,while,p27 presented with either cytoplasmic and/or nuclear reactivity in all studied *** analysis revealed a direct relation between Cyclin D1 and p21 in CHC cases(P<0.001) ,between Cyclin D1 and Cyclin E in HCCs(P<0.01);however,an inverserelationship was detected between Cyclin D1 and p21 or p27(P<0.001) and between p21 and Rb/p105(P<0.05) in HCCs. CONCLUSION:Upregulation of Cyclin D1 in CHC p

关键词： Chronic hepatitis C Liver cirrhosis Hepatocellular carcinoma Cell cycle Cyclin D1 Cyclin E p21 p27 Rb/p105

HER-2/Neu Status in Gastric Carcinomas in a Series of Egyptian Patients and Its Relation to Ki-67 Expression

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Open Journal of Pathology 2015年第4期5卷 101-113页

作者： Saba El-Gendi Iman Talaat mona.abdel-hadi Department of Pathology Faculty of Medicine Alexandria University Alexandria Egypt

Objective: HER-2/neu status in gastric carcinomas (GCs) has not been studied before in the Egyptian population. Materials and Methods: Seventy-three GCs were evaluated for the expression of HER-2/neu and Ki-67 using immunohistochemistry (IHC). Fluorescence in situ Hybridization (FISH) was done for HER2/neu IHC score 2+ cases. Results: Out of the 73 gastric carcinomas, 23 (31.5%) were score 0, 17 (23.3%) were score 1+, 23 (31.5%) were score 2+, and 10 (13.7%) were score 3+. FISH analysis revealed that the HER-2/neu gene was amplified in 11 out of the 23 cases (47.8%) scored 2+ by IHC. Therefore, the overall HER-2/neu positivity rate was 28.8% and it was significantly associated with higher T-stage and lymphovascular invasion (LVI). The Ki-67 expression rate ranged between 10% and 100%, with 84.9% of the cases (n = 62) featuring high Ki-67 scores. High Ki-67 score was significantly associated with male sex, tumor grade, and number of positive nodes. HER-2/neu protein expression correlated significantly with Ki-67 score. Twenty tumors showed combined HER-2/neu positivity and high Ki-67 score and were significantly associated with higher T-stage and occurrence of LVI, implying a more aggressive behavior. Conclusions: The rate of HER-2/neu positive GCs in our series simulates universal rates, thereby mandating routine evaluation of HER-2/neu status in all GCs submitted to our laboratory to benefit from trastuzumab therapy. Further studies on a larger number of GCs are required to prove that the concurrent HER-2/neu positivity and high Ki-67 score are markers of worse prognosis.

关键词： Egypt FISH Gastric Carcinoma HER-2/Neu Immunohistochemistry Ki-67

Thermoresponsive dendronized copolymers for protein recognitions based on biotin-avidin interaction

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Chinese Chemical Letters 2017年第4期28卷 832-838页

作者： Chunhua Zhou mona A.abdel-Rahman Wen Li Kun Liu Afang Zhang Department of Polymer Materials Shanghai University Chemistry Department Polymer Lab.109Faculty of ScienceAssiut University

Thermoresponsive biotinylated dendronized copolymers carrying dendritic oligoethylene glycol（OEG）pendants were prepared via free radical polymerization,and their protein recognitions based on biotin-avidin interaction *** first（PG1） and second generation（PG2） dendronized copolymers were designed to examine possible thickness effects on the interaction between biotin and *** from the outstanding thermoresponsive properties from OEG dendrons,these biotinylated cylindrical copolymers show characteristic thermoresponsive behavior which provides an envelope to capture avidin through switching temperatures above or below their phase transition temperatures（T_（cp）s）.Thus,the recognition of polymer-supported biotin with avidin was investigated with UV/vis spectroscopy and dynamic laser light *** contrast to the case for PG1,the increased thickness for copolymer PG2 hinders partially and inhibits the recognition of biotin moieties with avidin either below or above its T_（cp）.This demonstrates the significant architecture effects from dendronized polymers on the biotin moieties to shift onto periphery of the collapsed aggregates,which should be a prerequisite for protein *** kinds of novel thermoresponsive copolymers may pave a way for the interesting biological applications in areas such as reversible activity control of enzyme or proteins,and for controlled delivery of drugs or genes.

关键词： Dendronized copolymers Dendrimers Thermoresponsive polymers Protein recognition Biotin-avidin interaction Supramolecular chemistry

Chronic Hepatitis C Infection in Children: Current Treatment and New Therapies

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Journal of Clinical and Translational Hepatology 2015年第1期3卷 36-41页

作者： Andrew Lee Jeremy Rajanayagam mona.abdel-Hady Birmingham Children's Hospital Liver Unit Birmingham United Kingdom The University of Queensland School of MedicineBrisbane Australia Birmingham Children's Hospital Liver Unit Birmingham United Kingdom University of Birmingham School of Infection and Immunity Birmingham United Kingdom Birmingham Children's Hospital Liver Unit Birmingham United Kingdom

Viral hepatitis C is responsible for a large burden of disease *** of hepatitis C infection is currently undergoing a revolution with the development of new direct acting antivirals that offer higher cure rates and fewer side effects than other medications currently *** options for children,although well-defined and evidencebased,are limited relative to adults as there are few trials regarding the use of these newly developed agents in *** so much optimism in the development of novel therapeutic options for hepatitis C,it is timely to review and summarize the current standard of care treatment and indications for treatment of chronic hepatitis C in *** provide here an overview of recent drug developments and their potential for use in children.

关键词： Hepatitis C Children Treatment Direct acting antivirals Host targeting agents

Lipid Production,Oxidative Status,Antioxidant Enzymes and Photosynthetic Efficiency of Coccomyxa chodatii SAG 216-2 in Response to Calcium Oxide Nanoparticles

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Phyton-International Journal of Experimental Botany 2023年第7期92卷 1955-1974页

作者： Huwida A.A.abdel-Kader Naeima Yousef Mohammad Anwar Hossain mona F.A.Dawood Department of Botany&Microbiology Faculty of ScienceAssiut UniversityAssiut71516Egypt Department of Genetics and Plant Breeding Bangladesh Agricultural UniversityMymensingh2202Bangladesh

The extensive use of nanoparticles(NPs)in diverse applications causes their localization to aquatic habitats,affecting the metabolic products of primary producers in aquatic ecosystems,such as *** calcium oxide nanoparticles(CaO NPs)are of the scarcely studied ***,the current work proposed that the exposure to CaO NPs may instigate metabolic pathway to be higher than that of normally growing algae,and positively stimulate algal *** this respect,this research was undertaken to study the exposure effect of CaO NPs(0,20,40,60,80,and 100μg mL^(−1))on the growth,photosynthesis,respiration,oxidative stress,antioxidants,and lipid production of the microalga Coccomyxa chodatii SAG *** results showed that the algal growth concomitant with chlorophyll content,photosynthesis,and calcium content increased in response to CaO *** contents of biomolecules such as proteins,amino acids,and carbohydrates were also promoted by CaO NPs with variant ***,lipid production was enhanced by the applied *** NPs induced the accumulation of hydrogen peroxide,while lipid peroxidation was reduced,revealing no oxidative behavior of the applied nanoparticles on ***,CaO NPs have a triggering effect on the antioxidant enzymes such as superoxide dismutase,catalase,ascorbate peroxidase,and guaiacol *** results recommended the importance of the level of 60μg mL^(−1) CaO NPs on lipid production(with increasing percentage of 65%compared to control)and the highest dry matter acquisition of *** study recommended the feasibility of an integrated treatment strategy of CaO NPs in augmenting biomass,metabolic up-regulations,and lipid accumulation in ***.

关键词： Calcium oxide nanoparticles Coccomyxa chodatii growth hydrogen peroxide metabolic products

A Survey on Acute Leukemia Expression Data Classification Using Ensembles

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Computer Systems Science & Engineering 2023年第11期47卷 1349-1364页

作者： abdel Nasser H.Zaied Ehab Rushdy mona Gamal Department of Information Systems Faculty of Computer ScienceMisr International UniversityCairo11828Egypt Department of Information Technology Faculty of Computers and InformaticsZagazig UniversityZagazig44516Egypt Department of Information Systems Faculty of Computers and InformaticsZagazig UniversityZagazig44516Egypt

Acute leukemia is an aggressive disease that has high mortality rates *** error rate can be as high as 40%when classifying acute leukemia into its ***,there is an urgent need to support hematologists during the classification *** than two decades ago,researchers used microarray gene expression data to classify cancer and adopted acute leukemia as a test *** high classification accuracy they achieved confirmed that it is possible to classify cancer subtypes using microarray gene expression *** machine learning is an effective method that combines individual classifiers to classify new *** classifiers are recognized as powerful algorithms with numerous advantages over traditional *** the past few decades,researchers have focused a great deal of attention on ensemble classifiers in a wide variety of fields,including but not limited to disease diagnosis,finance,bioinformatics,healthcare,manufacturing,and *** paper reviews the recent ensemble classifier approaches utilized for acute leukemia gene expression data ***,a framework for classifying acute leukemia gene expression data is *** pairwise correlation gene selection method and the Rotation Forest of Bayesian Networks are both used in this *** outcomes show that the classification accuracy achieved by the acute leukemia ensemble classifiers constructed according to the suggested framework is good compared to the classification accuracy achieved in other studies.

关键词： Leukemia classification ensemble rotation forest pairwise correlation bayesian networks gene expression data microarray gene selection

A new empirical chart for rockburst analysis in tunnelling:Tunnel rockburst classification(TRC)

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International Journal of Mining Science and Technology 2021年第4期31卷 603-610页

作者： hadi Farhadian Department of Mining Engineering Faculty of EngineeringUniversity of BirjandBirjandIran

Rockburst is defined as a phenomenon with immediate dynamic instability under excavation unloading conditions of deep or high geostress *** knowledge and lack of characterizing information prevent engineers and experts from achieving appropriate prediction results related to the rockburst *** this study,a data set including 220 rockburst instances was collected for rockburst classification via the geostatistical *** update of the 2D graph,the tunnel rockburst classification(TRC)chart,was introduced based on analysing three indicators,namely,elastic energy index(Wet),tangential stress in rock mass(σ_(0)),and uniaxial compressive strength(σ_(c)).Distribution and correlation of data were drawn on 2D plot,and the boundaries of rockburst were distinguished according to the achieved interpolate points by kriging ***,the validation phase was performed using an additional set of 28 case histories obtained from several projects around the *** results showed that the TRC chart with an average error percentage of 3.6%in the prediction of rockburst had a significant and effective implementation in comparison to the exiting heuristic *** the initial character of the prediction,the described chart may be a helpful tool in the first steps of design and construction.

关键词： Prediction Tunnel rockburst Classification Empirical approach TRC

Experimental and numerical study on crack propagation in pre-cracked beam specimens under three-point bending

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Journal of Central South University 2016年第2期23卷 430-439页

作者： hadi Haeri Department of Mining Engineering Bafgh BranchIslamic Azad University

A simultaneous experimental and numerical study on crack propagation in the pre-cracked beams specimens(concrete-like materials) is carried out using three-point bending flexural test. The crack propagation and coalescence paths of internal cracks in side beam specimens are experimentally studied by inserting double internal cracks. The effects of crack positions on the fracturing path in the bridge areas of the double cracked beam specimens are also studied. It has been observed that the breaking of concrete-like cracked beams specimens occurs mainly by the propagation of wing cracks emanating from the tips of the pre-existing cracks in the numerical and experimental analyses, respectively. The same specimens are numerically simulated by an indirect boundary element method(IBEM) known as displacement discontinuity method(DDM) using higher displacement discontinuity. These numerical results are compared with the existing experimental results. This comparison illustrates the higher accuracy of the results obtained by the indirect boundary element method by using only a small number of elements compared with the discrete element method(PFC2D code).

关键词： double internal cracks concrete-like specimens crack propagation