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Journal of Tuberculosis Research 2013年第2期1卷 10-13页

作者： Asaad Maidin Agnes Lidjaja mochammad hatta Biopharmaceutical Laboratory Faculty of Pharmacy Hasanuddin University Makassar Indonesia Microbiology Department Faculty of Medicine Hasanuddin University Makassar Indonesia Molecular Biology and Immunology Laboratory Microbiology Department Faculty of Medicine Hasanuddin University Makassar Indonesia

Background: M. tuberculosisis the most important etiological factor of tuberculosis. One of the factors that make TB hard to eradicate is the emergence of M. tuberculosisdrug resistance. Drug resistance in M. tuberculosisis attributed primarily to the accumulation of mutations in the drug target gene. Objectives: to analyze profile of Random Amplified Polymorphic DNA (RAPD) in M. tuberculosisisolates resistant to Isoniazid and found RAPD marker. Methods: seven Isoniazid resistant isolate of M. tuberculosisfrom Ma kassar, Indonesia strain were analyzed by RAPD method using primers OPN 02, OPN 09, OPN 20, BG 65, N 9, that amplification fragment DNA than as molecular marker. Results: The results of the present study showed high degree of polymerphism in *** in the population, and found that specific DNA fragment at Isoniazid resistant isolates using primer N 9 is 1450 bp as a marker. Conclusion: This study gives information about RAPD marker of M. tuberculosis strain to Isoniazid resistant.

关键词： Mycobacterium tuberculosis RAPD Isoniazid Resistant

lnterleukin-6 and interleukin-10 plasma levels and mRNA expression in polytrauma patients

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Chinese Journal of Traumatology 2017年第6期20卷 318-322页

作者： Heber B. Sapan Idrus Paturusi Andi Asadul Islam Irawan Yusuf Ilhamjaya Patellongi Muhammmad Nasrum Massi Aryono D. Pusponegoro Syafrie K. Arief Ibrahim Labeda Leo Rendy mochammad hatta Department of Surgery Universitas Sam Ratulangi Faculty of Medicine Manado Indonesia Department of Orthopedics and Traumatology Universitas Hasanuddin Faculty of Medicine Makassar Indonesia Department of Surgery Universitas Hasanuddin Faculty of Medicine Makassar Indonesia Department of Physiology Universitas Hasanuddin Faculty of Medicine Makassar Indonesia Department of Microbiology Universitas Hasanuddin Faculty of Medicine Makassar Indonesia Department of Surgery Universitas Indonesia Faculty of Medicine Jakarta Indonesia Department of Anesthesiology Universitas Hasanuddin Faculty of Medicine Makassar Indonesia Molecular Biology and Immunology Laboratory Universitas Hasanuddin Faculty of Medicine Makassar Indonesia

OBJECTIVE:Host response to polytrauma occasionally has unpredictable outcomes. Immune response is a major factor influencing patient's outcome. This study evaluated the interaction of two main cytokines in immune response after major trauma, specifically interleukin-6 (IL-6) and interleukin-10 (IL-10). Plasma level of these cytokines is determined by mRNA expression of these cytokines genes which may decide the outcome of polytrauma patients.;METHODS:This prospective multicenter trial held at four trauma centers enrolled 54 polytrauma patients [Injury Severity Score (ISS) ≥ 16]. Plasma levels and mRNA expression of IL-6 and IL-10 were measured for 5 days after trauma. Clinical evaluation was conducted to observe whether patients endured multiple organ dysfunction syndrome (MODS) and death. MODS evaluation was performed using sequential organ failure assessment (SOFA). Trauma load which in this study is represented with ISS, plasma level, expression of cytokine genes and patient's outcome were examined with correlation test and statistical analysis.;RESULTS:The elevated IL-6/IL-10 ratio indicated increased activity of systemic inflammation response, especially pro-inflammation response which bears higher probability of progressing to MODS and death. The decline of IL-6/IL-10 ratio with heavy trauma load (ISS > 30) showed that compensatory anti-inflammation response syndrome (CARS) state was more dominant than systemic inflammatory response syndrome (SIRS), indicating that malfunction and failure of immune system eventually lead to MODS and deaths. The statistical significance in plasma level of cytokines was found in the outcome group which was defined as bearing a low trauma load but mortality.;CONCLUSIONS:The pattern of cytokine levels in inflammation response has great impact on the outcome of polytrauma patients. Further study at the genetic level is needed to investigate inflammation process which may influence patient's outcome.

关键词： Interleukine-10 Interleukine-6 Multiple organ dysfunction syndrome Polytrauma mRNA expression

The Role of Cytokine Interleukin-2, Transcription Factor of FoxP3 in the Immunological Regulation of Allergic Rhinitis

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International Journal of Otolaryngology and Head & Neck Surgery 2018年第1期7卷 7-19页

作者： Bambang Suprayogi Resi Utomo mochammad hatta Robert H. Sirait Sutji Pratiwi Muhammad Nasrum Massi Department of Ear Nose and Throat Faculty of Medicine Indonesia Christian University of Jakarta Jakarta Indonesia Molecular Biology and Immunology Laboratory for Infection Diseases Faculty of Medicine University of Hasanuddin Makassar Indonesia Department of Anesthesiology Faculty of Medicine Indonesia Christian University of Jakarta Jakarta Indonesia Department of Ear Nose and Throat Faculty of Medicine University of Hasanuddin Makassar Indonesia Department of Microbiology Faculty of Medicine University of Hasanuddin Makassar Indonesia

Allergic rhinitis (AR) is the inflammation of nasal mucosa due to the type 1 hypersensitivity reactions mediated by immunoglobulin E (IgE) and triggered by certain allergens. The latest concept in allergic disease is the role of regulatory T cells (Treg). Interleukin-2 enhances the function and survival of Treg to perform its function as a controller of effector for forming a tolerant system by suppressing and regulating the homeostasis system. Treg has a transcription factor FoxP3 which plays a role in developing major function of Treg and progression to produce IL-10 and TGF-?. The atopic diseases are caused by a deficiency of Treg. The new perspective is low-dose IL-2 therapy towards autoimmune disease and allergic inflammation. Low-dose IL-2 therapy requires further clinical studies to optimize the dose, time, and the schedule of the IL-2 treatment. FoxP3 has the potential to assist in evaluating the active process of immunological process, which cannot be evaluated by Th1 and Th2 markers, and FoxP3 can be a successful immunotherapy marker.

关键词： Allergic Rhinitis Interleukin-2 FoxP3

Analysis of Forkhead Box Protein-3 (Foxp3) in Allergic Rhinitis Patients

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International Journal of Otolaryngology and Head & Neck Surgery 2018年第4期7卷 228-236页

作者： Bambang Suprayogi Resi Utomo mochammad hatta Sutji Pratiwi Muhammad Nasrum Massi Lina Marlina Erica Gilda Minawati Simanjuntak Department of Ear Nose and Throat Faculty of Medicine Indonesia Christian University of Jakarta Jakarta Indonesia Molecular Biology and Immunology Laboratory for Infection Diseases Faculty of Medicine University of Hasanuddin Makassar Indonesia Department of Ear Nose and Throat Faculty of Medicine University of Hasanuddin Makassar Indonesia Department of Microbiology Faculty of Medicine University of Hasanuddin Makassar Indonesia Department of Anesthesiology Faculty of Medicine Indonesia Christian University of Jakarta Jakarta Indonesia

Background: A new concept in understanding allergic diseases is regulatory T cells (Tregs), which control the immune reaction caused by Th2 cytokine production. Forkhead Box Protein 3 (FoxP3) is a marker that has a critical role in the development and function of Tregs. Some studies found differences in FoxP3 level and in Tregs capacity to control immune reactions in allergic diseases. The aim of this study was to investigate FoxP3 level in Allergic rhinitis (AR) patients compared to atopic and healthy/normal persons in Jakarta. Methodology: This study used observation to analyze the level of FoxP3 in AR, atopic and healthy/normal persons, and used ELISA to measure the FoxP3 level. Results: The study had sixty participants divided into three groups: 21 in the Normal group, 16 in the Atopic group, and 23 in the AR group. The mean FoxP3 levels were 0.81 ± 0.35 in the normal group, 3.42 ± 0.15 in the atopic group, and 3.40 ± 0.13 in the AR group. Statistical analysis with Mann-Whitney tests indicated significant differences, with AR and atopic groups having higher FoxP3 levels than the normal group, (p = 0.001), and no statistically significant differences between the AR and atopic groups, (p = 0.92). Conclusion: Our study results suggested that FoxP3 was active in the control of inflammatory processes due to allergies, and decrease level of FoxP3 indicated severe AR, but suggested another mechanism caused differences in the clinical phenotypes of AR and atopic patients, despite them having equally high levels of FoxP3.

关键词： Allergic Rhinitis Atopic Regulator T Cells FoxP3 ELISA

The Analysis of the Effective Systemic Lidocaine Dosage on the Expression of HMGB1 mRNA on Mice with Sterile Musculoskeletal Injury

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Open Journal of Anesthesiology 2017年第2期7卷 35-41页

作者： Robert Hotman Sirait mochammad hatta Muhammad Ramli Tigor Peniel Simanjuntak Bambang Suprayogi Andi Asadul Islam Syafrie Kamsul Arief Department of Anesthesiology Faculty of Medicine Christian University of Indonesia Jakarta Indonesia Molecular Biology and Immunology Laboratory Faculty of Medicine University of Hasanuddin Makassar Indonesia Department of Anesthesiology Faculty of Medicine University of Hasanuddin Makassar Indonesia Department of Obstetric and Gynecology Faculty of Medicine Christian University of Indonesia Jakarta Indonesia Department of Otorhinolaryngology Faculty of Medicine Christian University of Indonesia Jakarta Indonesia Department of Surgery Faculty of Medicine University of Hasanuddin Makassar Indonesia

A severe injury can trigger an inflammation response and excessive response can cause multiple organ failure. HMGB1 is an early inflammation mediator in sterile injury and a late inflammation mediator in infection. It is an important mediator in severe sepsis and always associated with the severity of organ failure. Previous studies showed that the administration of systemic lidocaine could inhibit the expression of HMGB1 on septic mice with musculoskeletal injury. Nine male adult Balb/c mice were grouped by simple random sampling method into three groups of intravenous lidocaine injection dosages: 2 mg/kg, 3 mg/kg, 4 mg/kg. Musculoskeletal injury was done by breaking the left femoral bone in a close manner. Peripheral blood sampling was done 4 hours after injury and 2 hours after lidocaine therap. To evaluate the expression level of HMGB1 mRNA, RT-PCR was used. The result of our study showed that intravenous lidoaine administration on the 3 groups could decrease the level of HMGB1. In conclusion, lidocaine hold an important role in clinical diseases by inhibiting HMGB1.

关键词： High Mobility Group Box 1 Lidocaine Musculoskeletal Injury

Analysis Concentration of <i>Toxoplasma gondii</i>on Anti-Toxoplasma IgG-IgM Antibody Levels, and the Outcomes of Pregnancy in Mice Balb/c

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Open Journal of Obstetrics and Gynecology 2017年第3期7卷 281-289页

作者： Tigor Peniel Simanjuntak mochammad hatta Robert H. Sirait Marni Br Karo Lenny Irmawaty Sirait Tetty Rina Aritonang Syahrul Rauf Ressy Dwiyanti Department of Obstetrics and Gynecology Faculty of Medicine Christian University of Indonesia Jakarta Indonesia Molecular Biology and Immunology Laboratory Faculty of Medicine Hasanuddin University Makassar Indonesia Department of Anaestesiology Faculty of Medicine Christian University of Indonesia Jakarta Indonesia Midwife Program of Medistra Health Higher School Jakarta Indonesia Department of Obstetrics and Gynecology Faculty of Medicine Hasanuddin University Makassar Indonesia Department of Medical Microbiology Faculty of Medicine Tadulako University Palu Indonesia

Introduction: Toxoplasma gondii is an obligate intracellular protozoan parasite that can infect any warm blood vertebrae, and if first trimester pregnant woman infected, it may cause abortion. The objective is to prove the effect of the Toxoplasma gondii concentration in anti-toxoplasma IgG-IgM antibody levels, and the outcomes of Balb/c mice pregnancies. Materials and Methods: The study was conducted in Balb/c mice with inclusion criteria, and was conditioned pregnant. The pathogen strains of Toxoplasma gondii tachyzoite injected intraperitoneally. The blood samples were taken serially to be tested for anti-toxoplasma IgG-IgM antibody levels. After the mice were injected with tachyzoite, they are assessed every day to observe their body weight, vaginal bleeding, and labor. Anti-toxoplasma IgG-IgM antibody levels examined using qualitative mouse IgG-IgM antibody ELISA KIT. Results: Anti-toxoplasma IgM antibody levels increased significantly after 24 hours of injection tachyzoites in all dose groups, and remained high through day 21. Anti-toxoplasma antibody IgG levels increased significantly after 72 hours post injection and remained elevated until day 21. The incidence of abortion is 100% in mice which injected tachyzoite levels 1 × 103 and 1 × 104, and the incidence of abortion approximately 2 - 4 days post injection. 100% of mice that were injected with tachyzoites 1 × 101 and 1 × 102 have labor at term. Physical anomaly was found in baby mice from mice that were injected with tachyzoite 1 × 102. Conclusion: There is a significant correlation between the concentrations of Toxoplasma gondii tachyzoite with anti-toxoplasma IgG-IgM antibody levels, and there is a significant relationship between the concentrations of tachyzoite with abortion.

关键词： Toxoplasma gondii Tachyzoite Anti-Toxoplasma IgG-IgM Antibody Levels Pregnancy Abortion Labor

Prediction of hereditary nonpolyposis colorectal cancer using mRNA MSH2 quantitative and the correlation with nonmodifiable factor

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World Journal of Gastrointestinal Pathophysiology 2021年第6期12卷 134-146页

作者： Tjahjadi Robert Tedjasaputra mochammad hatta Muh Nasrum Massi Rosdiana Natzir Agussalim Bukhari Rina Masadah Muh Lutfi Parewangi Prihantono Prihantono Rinda Nariswati Vincent Tedjasaputra Department of Internal Medicine Tarakan General HospitalMedical Faculty University of HasanuddinJakarta 10720DKI JakartaIndonesia Department of Immunology and Biomolecular Hasanuddin UniversityMakassar 90245South SulawesiIndonesia Department of Microbiology Faculty of MedicineUniversity of HasanuddinMakassar 90245South SulawesiIndonesia Department of Biochemistry Meidcal Faculty University of HasanuddinMakassar 90245South SulawesiIndonesia Department of Nutrition Faculty of MedicineHasanuddin UniversityMakassar 90245South SulawesiIndonesia Department of Pathology Anatomy Faculty of MedicineHasanuddin UniversityMakassar 20945South SulawesiIndonesia Department of Internal Medicine Faculty of MedicineHasanuddin UniversityMakassar 20945South SulawesiIndonesia Department of Surgery Faculty of MedicineHasanuddin UniversityMakassar 90245South SulawesiIndonesia Department of Statistic School of Computer ScienceBina Nusantara University JakartaJakarta 11530Indonesia American Association for the Advancement of Science(AAAS) Science and Technology Policy FellowAlexandriaVA 22314United States

BACKGROUND Hereditary non-polyposis colon cancer is a dominantly inherited syndrome of colorectal cancer(CRC),with heightened risk for younger *** studies link its susceptibility to the DNA sequence polymorphism along with Amsterdam and Bethesda ***,those fail in term of *** To determine a clear cut-off of MSH2 gene expression for CRC heredity grouping ***,the study also aims to examine the association of risk factors to the CRC *** The cross-sectional study observed 71 respondents from May 2018 to December 2019 in determining the CRC hereditary status through MSH2 mRNA expression using reverse transcription-polymerase chain reaction and the disease’s risk *** were analyzed through Chi-Square,Fischer exact,t-test,Mann-Whitney,and multiple *** There are significant differences of MSH2 within CRC group among tissue and blood;yet,negative for significance between *** the blood gene expression fifth percentile,the hereditary CRC cut-off is 11059 fc,dividing the 40 CRC respondents to 32.5%with hereditary *** risk factors include age,family history,and ***,after multivariate control,age is just a ***,the study develops a probability equation with area under the curve 82.2%.CONCLUSION Numerous factors have significant relations to heredity of CRC ***,true important factors are staging and family history,while age and others are *** study also established a definite cut-off point for heredity CRC based on mRNA MSH2 expression,11059 *** findings shall act as concrete foundations on further risk factors and/or genetical CRC future studies.

关键词： Colorectal cancer MSH2 gene Non-modifiable factors Risk probability

Effectivity and efficacy probiotics for Bacterial Vaginosis treatments:Meta-analysis

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Infectious Disease Modelling 2022年第4期7卷 597-604页

作者： Sri Adila Nurainiwati mochammad Ma'roef Dwi Nurwulan Pravitasari Probo Yudha Pratama Putra Department Dermatology and Venereology Medical Faculty University of Muhammadiyah MalangMalangIndonesia Department Obstetric and Gynecology Medical FacultyUniversity of Muhammadiyah MalangMalangIndonesia Medical Faculty University of Muhammadiyah Malang MalangIndonesia

Introduction:Bacterial Vaginosis(BV)is the most common cause of vaginal ***,in some cases,side effects and resistance rates have been reported when anti-biotics are *** problem has prompted several investigations on the administration of probiotics as an adjunct therapy to treat this ***:This study aims to conduct a meta-analysis based on evidence to determine the efficacy and safety of probiotic and antibiotic ***:The meta-analysis was performed using PRISMA *** literature review was conducted in December 2020 using PubMed,Science Direct,Cochrane Library,and RevMan ***:The results showed a high and significant cure rate from the analysis of 1006 and 528 samples of probiotics and non-probiotics or control in 16 *** recurrence rate was statistically significant with probiotic ***,neither procedures nor therapy failure showed a significantly lower adverse event rate than the control ***:Probiotic shows better results compared to the control ***,both have the same occurrence of adverse event.

关键词： Antibiotics Bacterial Vaginosis Probiotics

Scanometry as microplate reader for high throughput method based on DPPH dry reagent for antioxidant assay

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Acta Pharmaceutica Sinica B 2017年第3期7卷 395-400页

作者： mochammad Amrun Hidayat Aulia Fitri Bambang Kuswandi Chemo and Biosensor Group Faculty of PharmacyUniversity of JemberJember 68121Indonesia

The stable chromogenic radical 1,1'-diphenyl-2-picrylhydrazyl(DPPH) solution was immobilized on the microwell plate as dry reagent to construct a simple antioxidant sensor. Then, a regular flatbed scanner was used as microplate reader to obtain analytical parameters for antioxidant assay using one-shot optical sensors as scanometry technique. Variables affecting the acquisition of the images were optimized and the analytical parameters are obtained from an area of the sensing zone inside microwell using the average luminosity of the sensing zone captured as the mean of red, green, and blue(RGB)value using Image J~program. By using this RGB value as sensor response, it is possible to determine antioxidant capacity in the range 1–25 ppm as gallic acid equivalent(GAE) with the response time of 9 min. The reproducibility of sensor was good(RSD<1%) with recovery at 93%–96%. The antioxidant sensor was applied to the plant extracts, such as sappan wood and Turmeric Rhizome. The results are good when compared to the same procedure using a UV/Vis spectrophotometer.

关键词： Scanometry High throughput DPPH Optical sensor Antioxidant

Delayed versus immediate intervention of ruptured brain arteriovenous malformations:A case report

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World Journal of Clinical Cases 2023年第9期11卷 1992-2001页

作者： Andi Kurnia Bintang Ashari Bahar Muhammad Akbar Gita Vita Soraya Anthony Gunawan Nurussyariah Hammado mochammad Erwin Rachman Zulvikar Syambani Ulhaq Department of Neurology Hasanuddin UniversityMakassar 90245Indonesia Department of Biochemistry Faculty of MedicineHasanuddin UniversityMakassar 90245Indonesia Faculty of Health and Sport Science State University of MakassarMakassar 90222Indonesia Faculty of Medicine Muslim University of IndonesiaMakassar 90231Indonesia Research Center for Preclinical and Clinical Medicine National Research and Innovation Agency Republic of IndonesiaCibinong 16911Indonesia Laboratory of Developmental Disorders and Toxicology Kyushu UniversityFukuoka 8190395Japan Department of Biomedical Science Faculty of Medicine and Health SciencesMaulana Malik Ibrahim State Islamic UniversityMalang 65144Indonesia

BACKGROUND Brain arteriovenous malformations(bAVMs)remains one of the most prevalent causes of intracranial hemorrhage and stroke-like syndromes in the young adult *** it has been agreed upon that definitive treatment using either single or multi-modal approach is warranted for successful bAVM management,much debate still revolves regarding the optimal timing of definitive *** SUMMARY In this report,we present a case of delayed,definitive endovascular treatment for ruptured bAVM in a 21-year-old female,3 mo *** bAVM,with a left pericallosal feeding artery and cortical draining veins,was successfully obliterated through embolization using the Onyx *** follow-up the patient has recommenced her daily activities and experiences only mild occasional headaches with mild motor *** report leads to our review on an important issue regarding the optimal timing of ruptured bAVM definitive management and bring forward the current evidence available on delayed vs immediate definitive bAVM *** also highlight current issues that need to be addressed for clearer guidelines on definitive therapy *** Current treatment paradigms of ruptured bAVM remains elusive,with substantial heterogeneity in the current literature.A consensus on the definition of“acute”vs“delayed”,management goal,follow-up length and outcome parameters are required to support formation of a clear paradigm.

关键词： Brain arteriovenous malformation Ruptured brain arteriovenous malformation Definitive intervention Endovascular treatment Case report