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Photonic control of ligand nanospacing in self-assembly regulates stem cell fate

Bioactive Materials 2024年第4期34卷 164-180页

作者： Sungkyu Lee Jounghyun Yoo Gunhyu Bae Ramar Thangam Jeongyun Heo Jung Yeon Park Honghwan Choi Chowon Kim Jusung An Jungryun Kim Kwang Rok Mun Seungyong Shin Kunyu Zhang Pengchao Zhao Yuri Kim Nayeon kang Seong-Beom Han Dahee Kim Jiwon Yoon misun kang Jihwan Kim Letao Yang Solmaz Karamikamkar Jinjoo Kim Yangzhi Zhu Alireza Hassani Najafabadi Guosheng Song Dong-Hwee Kim Ki-Bum Lee Soong Ju Oh Hyun-Do Jung Hyun-Cheol Song Woo Young Jang Liming Bian Zhiqin Chu Juyoung Yoon Jong Seung Kim Yu Shrike Zhang Yongju Kim Ho Seong Jang Sehoon Kim Heemin kang Department of Materials Science and Engineering Korea UniversitySeoul02841Republic of Korea Chemical and Biological Integrative Research Center Korea Institute of Science and TechnologySeoul02792Republic of Korea KU-KIST Graduate School of Converging Science and Technology Korea UniversitySeoul02841Republic of Korea Department of Chemistry Korea UniversitySeoul02841Republic of Korea Materials Architecturing Research Center Korea Institute of Science and TechnologySeoul02792Republic of Korea School of Biomedical Sciences and Engineering Guangzhou International CampusSouth China University of TechnologyGuangzhou511442China Department of Chemistry and Chemical Biology Rutgers UniversityPiscatawayNJ08854USA Terasaki Institute for Biomedical Innovation Los AngelesCA90064USA State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityChangsha410082China Department of Biomedical-Chemical Engineering The Catholic University of KoreaGyeonggi-do14662Republic of Korea Electronic Materials Research Center Korea Institute of Science and TechnologySeoul02792Republic of Korea KIST-SKKU Carbon-Neutral Research Center Sungkyunkwan University(SKKU)Suwon16419Republic of Korea Department of Orthopedic Surgery Korea University Anam HospitalSeoul02841Republic of Korea Department of Electrical and Electronic Engineering and Joint Appointment with School of Biomedical Sciences The University of Hong KongHong Kong518057China Department of Chemistry and Nanoscience Ewha Womans UniversitySeoul03760Republic of Korea Division of Engineering in Medicine Department of MedicineBrigham and Women’s Hospital Harvard Medical SchoolCambridgeMA02139USA Division of Nano&Information Technology KIST SchoolKorea University of Science and Technology(UST)Seoul02792Republic of Korea College of Medicine Korea UniversitySeoul02841Republic of Korea Division of Materials Science and Engineering Hanyang UniversitySeoul04763Republic of Korea

Extracellular matrix(ECM)undergoes dynamic inflation that dynamically changes ligand nanospacing but has not been *** we utilize ECM-mimicking photocontrolled supramolecular ligand-tunable Azo^(+)self-assembly composed of azobenzene derivatives(Azo^(+))stacked via cation-πinteractions and stabilized with RGD ligand-bearing poly(acrylic acid).Near-infrared-upconverted-ultraviolet light induces cis-Azo^(+)-mediated inflation that suppresses cation-πinteractions,thereby inflating liganded *** inflation increases nanospacing of“closely nanospaced”ligands from 1.8 nm to 2.6 nm and the surface area of liganded selfassembly that facilitate stem cell adhesion,mechanosensing,and differentiation both in vitro and in vivo,including the release of loaded molecules by destabilizing water bridges and hydrogen bonds between the Azo^(+)molecules and loaded ***,visible light induces trans-Azo^(+)formation that facilitates cation-πinteractions,thereby deflating self-assembly with“closely nanospaced”ligands that inhibits stem cell adhesion,mechanosensing,and *** stark contrast,when ligand nanospacing increases from 8.7 nm to 12.2 nm via the inflation of self-assembly,the surface area of“distantly nanospaced”ligands increases,thereby suppressing stem cell adhesion,mechanosensing,and ***-term in vivo stability of self-assembly via real-time tracking and upconversion are *** tuning of ligand nanospacing can unravel dynamic ligand-cell interactions for stem cell-regulated tissue regeneration.

关键词： Dynamic self-assembly Ligand nanospacing In vivo tracking Stem cell adhesion Stem cell fate

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Formic acid as an alternative reducing agent for the catalytic nitrate reduction in aqueous media

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Journal of Environmental Sciences 2013年第8期25卷 1696-1702页

作者： Eun-kyoung Choi Kuy-hyun Park Ho-bin Lee misun Cho Samyoung Ahn Department of Environmental Education Sunchon National University

Formic acid was used for the nitrate reduction as a reductant in the presence of Pd：Cu/γ-alumina catalysts. The surface characteristics of the bimetallic catalyst synthesized by wet impregnation were investigated by SEM, TEM-EDS. The metals were not distributed homogeneously on the surface of catalyst, although the total contents of both metals in particles agreed well with the theoretical values. Formic acid decomposition on the catalyst surface, its influence on solution pH and nitrate removal efficacy was investigated. The best removal of nitrate （50 ppm） was obtained under the condition of 0.75 g/L catalyst with Pd：Cu ratio （4：1） and two fold excess of formic acid. Formic acid decay patterns resembled those of nitrate removal, showing a linear relationship between kf （formic acid decay） and k （nitrate removal）. Negligible amount of ammonia was detected, and no nitrite was detected, possibly due to buffering effect of bicarbonate that is in situ produced by the decomposition of formic acid, and due to the sustained release of H2 gas.

关键词： formic acid catalytic nitrate reduction Pd:Cu/γ-alumina catalyst H2 supply

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Modeling and measuring glucose diffusion and consumption by colorectal cancer spheroids in hanging drops using integrated biosensors

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Microsystems & Nanoengineering 2022年第1期8卷 193-210页

作者： Nassim Rousset Rubén López Sandoval Mario Matteo Modena Andreas Hierlemann Patrick M.misun ETH Zürich Department of Biosystems Science and EngineeringBio Engineering LaboratoryMattenstrasse 26CH-4058 BaselSwitzerland

As 3D in vitro tissue models become more pervasive,their built-in nutrient,metabolite,compound,and waste gradients increase biological relevance at the cost of analysis *** these gradients and the resulting metabolic heterogeneity requires invasive and time-consuming *** alternative is using electrochemical biosensors and measuring concentrations around the tissue model to obtain size-dependent metabolism *** our hanging-dropintegrated enzymatic glucose biosensors,we conducted current measurements within hanging-drop compartments hosting spheroids formed from the human colorectal carcinoma cell line *** developed a physics-based mathematical model of analyte consumption and transport,considering(1)diffusion and enzymatic conversion of glucose to form hydrogen peroxide(H_(2)O_(2))by the glucose-oxidase-based hydrogel functionalization of our biosensors at the microscale;(2)H_(2)O_(2)oxidation at the electrode surface,leading to amperometric H_(2)O_(2)readout;(3)glucose diffusion and glucose consumption by cancer cells in a spherical tissue model at the microscale;(4)glucose and H_(2)O_(2)transport in our hangingdrop compartments at the macroscale;and(5)solvent evaporation,leading to glucose and H_(2)O_(2)*** model relates the measured currents to the glucose concentrations generating the *** low limit of detection of our biosensors(0.4±0.1μM),combined with our current-fitting method,enabled us to reveal glucose dynamics within our *** measuring glucose dynamics in hanging-drop compartments populated by cancer spheroids of various sizes,we could infer glucose distributions within the spheroid,which will help translate in vitro 3D tissue model results to in vivo.

关键词： diffusion hanging simplicity

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Comparison of the low-density lipoprotein cholesterol target value and the preventive effect of statins in elderly patients and younger patients

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Journal of Geriatric Cardiology 2017年第6期14卷 383-391页

作者： Akihiro Endo Taiji Okada misun Pak Yuzo Kagawa Shimpei Ito Hirotomo Sato Kenji Kageshima Yasuyuki Yoshida Kazuaki Tanabe Division of Cardiology Shimane University Faculty of Medicine Izumo Japan Division of Cardiology Tottori Prefectural Central Hospital Tottori Japan

Objective To assess whether the low-density lipoprotein cholesterol （LDL-C） target value and preventive effect of statins are different between elderly and younger patients. Methods We investigated 304 patients with previous percutaneous coronary intervention who underwent coronary angiography from January 2007 to December 2016 for examination of recurrent ischemia beyond the early restenosis. Patients were classified into two groups： age ≥ 75 years （elderly group： n = 140） and 〈 75 years （younger group： n = 164）. Relationships between the achieved LDL-C level, incidence of late coronary events, and the effectiveness of statins were evaluated. Results During follow-up, 179 patients underwent late coronary revascularization. Recurrent ischemia presenting as acute coronary syndrome （ACS） occurred in 83 cases. Kaplan-Meier curve analysis revealed that in the younger group, recurrent ACS was significantly lower in patients with LDL-C 〈 70 mg/dL than in those with LDL-C ranging from 70 to 〈 100 mg/dL （P = 0.035）; however, there was no difference between these in the elderly group （P = 0.863）. Instead, recurrent ACS was less frequent in patients with LDL-C ranging from 70 mg/dL to 〈 100 mg/dL than in those with LDL-C ≥ 100 mg/dL in the elderly group （v = 0.033）. Statin use was associated with decreased recurrent ACS （P = 0.005）; moreover, only using statins was an independent predictor in the elderly group （HR： 0.375; P = 0.007）. Conclusions Strict control of LDL-C to 〈 70 mg/dL was effective for reducing the incidence of recurrent ACS in younger patients. However, LDL-C 〈 100 mg/dL might be sufficient as the target value of LDL-C-lowering therapy for secondary prevention of ischemic events in Japanese elderly patients.

关键词： Acute coronary syndrome Late coronary event Prevention

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Multi-analyte biosensor interface for real-time monitoring of 3D microtissue spheroids in hanging-drop networks

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Microsystems & Nanoengineering 2016年第1期2卷 204-212页

作者： Patrick M.misun Jörg Rothe Yannick R.F.Schmid Andreas Hierlemann Olivier Frey ETH Zürich Department of Biosystems Science and EngineeringBio Engineering LaboratoryMattenstrasse 26Basel CH-4058Switzerland

Microfluidics is becoming a technology of growing interest for building microphysiological systems with integrated read-out *** we present the integration of enzyme-based multi-analyte biosensors into a multi-tissue culture platform for‘body-on-a-chip’*** microfluidic platform is based on the technology of hanging-drop networks,which is designed for the formation,cultivation,and analysis of fluidically interconnected organotypic spherical three-dimensional(3D)microtissues of multiple cell *** sensor modules were designed as small glass plug-ins featuring four platinum working electrodes,a platinum counter electrode,and an Ag/AgCl reference *** were placed directly into the ceiling substrate from which the hanging drops that host the spheroid cultures are *** electrodes were functionalized with oxidase enzymes to enable continuous monitoring of lactate and glucose through *** biosensors featured high sensitivities of 322±41 nA mM^(−1) mm^(−2) for glucose and 443±37 nA mM^(−1) mm^(−2) for lactate;the corresponding limits of detection were below 10μ*** proposed technology enabled tissue-size-dependent,real-time detection of lactate secretion from single human colon cancer microtissues cultured in the hanging ***,glucose consumption and lactate secretion were monitored in parallel,and the impact of different culture conditions on the metabolism of cancer microtissues was recorded in real-time.

关键词： biosensor body on a chip glucose hanging drop lactate metabolism spheroid

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Optimizing CT for the evaluation of vestibular aqueduct enlargement:Inter-rater reproducibility and predictive value of reformatted CT measurements

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Journal of Otology 2015年第1期10卷 13-17页

作者： misun Hwang Ryan Marovich Samuel S.Shin David Chi Barton F.Branstetter IV Department of Radiology University of Pittsburgh Medical Center Department of Otolaryngology University of Pittsburgh Medical Center Department of Neurosurgery University of Pittsburgh Medical Center Department of Otolaryngology Children's Hospital of Pittsburgh

Enlarged vestibular aqueduct(EVA), the most frequent identifiable cause of congenital hearing loss, is evaluated with high-definition multidetector CT in the axial plane. Our purpose was to determine which reformatted CT measurements are most reproducible. Seven multiplanar reformatted images were created for each of the 64 temporal bones in patients with EVA. Intraclass correlation coefficients(ICC) were used to assess inter-observer variability, and both linear regression and ROC analyses were used to compare the measurements with severity of hearing loss, as assessed by pure tone audiometry. All seven measurements had excellent inter-observer variability, with average-measure ICC ranging from 0.92 to 0.98. There was no statistically significant correlation between the radiologic degree of aqueduct enlargement and severity of hearing loss using any of the seven measurements; ROC analyses revealed areas under the curves ranging from 0.57 to 0.73. Optimal accuracy was obtained with a threshold of 1.75 mm as measured at the aqueductal aperture in the P€oschl plane, with sensitivity of 0.75 and specificity of0.63. Although the radiologic measurement may not serve as a reliable tool for assessing severity of EVA, P€oschl plane reformatting has proven to be better than conventional axial acquisition plane for identifying patients with clinically significant hearing loss.

关键词： Enlarged vestibular aqueduct Hearing loss Computed

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