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Journal of Integrative Plant Biology 2021年第11期63卷 1937-1951页

作者： lin Chen Fei He Ruicai Long Fan Zhang mingna li Zhen Wang Junmei Kang Qingchuan Yang Institute of Animal Science Chinese Academy of Agricultural SciencesBeijing 100193China

Alfalfa(Medicago sativa L.) is an important forage crop worldwide. However, little is known about the effects of breeding status and different geographical populations on alfalfa improvement. Here, we sequenced 220 alfalfa core germplasms and determined that Chinese alfalfa cultivars form an independent group, as evidenced by comparisons of FSTvalues between different subgroups, suggesting that geographical origin plays an important role in group differentiation. By tracing the influence of geographical regions on the genetic diversity of alfalfa varieties in China, we identified 350 common candidate genetic regions and 548 genes under selection. We also defined 165 loci associated with 24 important traits from genome-wide association studies. Of those, 17 genomic regions closely associated with a given phenotype were under selection, with the underlying haplotypes showing significant differences between subgroups of distinct geographical origins. Based on results from expression analysis and association mapping,we propose that 6-phosphogluconolactonase(MsPGL) and a gene encoding a protein with NHL domains(MsNHL) are critical candidate genes for root growth. In conclusion, our results provide valuable information for alfalfa improvement via molecular breeding.

关键词： alfalfa genome-wide association studies genomic selection geographical origin population structure root development

Should they wait?Two children under 3 years old infected by HCV 1b successfully treated by ledipasvir/sofosbuvir:A report of two cases

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liver Research 2023年第4期7卷 361-364页

作者： mingna li Kuerbannisa Wulayin Shutao lin Chao Wu Lubiao Chen Department of Infectious Diseases The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdongChina Department of Infectious Diseases The First People’s Hospital of KashiKashiXinjiangChina

Although direct-acting antivirals(DAAs)have notably increased the sustained virological response(SVR)rates in hepatitis C virus(HCV)-infected adolescent patients,the efficacy and safety for young children under 3 years old remain ***,no guidelines recommend DAA therapy for this situation ***,the China National Medical Products Administration has not approved any DAA for treating children below 12 years ***,we described the characteristics of two children approximately 2 years old,who were infected by HCV genotype 1b and had significant clinical *** received 12 weeks of ledipasvir/sofosbuvir(Case 1:45.00 mg/200 mg per day,weight 17 kg;Case 2:33.75 mg/150 mg per day,weight 12 kg).They achieved SVR at 12 weeks after treatment completion without obvious treatment-related adverse ***,the safety and benefits of ledipasvir/sofosbuvir treatment in children under 3 years old seem to be *** findings require further evaluation.

关键词： Hepatitis C virus(HCV) Ledipasvir(LDV) Sofosbuvir(SOF) Direct-acting antivirals(DAAs) Genotype 1b Children

Correlation between radiologic features on contrastenhanced CT and pathological tumor grades in pancreatic neuroendocrine neoplasms

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The Journal of Biomedical Research 2021年第3期35卷 179-188页

作者： Wenbin Xu Han Yan Lulu Xu mingna li Wentao Gao Kuirong Jiang Junli Wu Yi Miao Pancreas Center the First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Pancreas Institute of Nanjing Medical University NanjingJiangsu 210029China Department of Radiology the First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Pathology the First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China

Contrast-enhanced computed tomography(CT)contributes to the increasing detection of pancreatic neuroendocrine neoplasms(PNENs).Nevertheless,its value for differentiating pathological tumor grades is not well *** this report,we have conducted a retrospective study on the relationship between the 2017 World Health Organization(WHO)classification and CT imaging features in 94 *** of the investigated features eventually provided statistically significant indicators for discerning PNENs G3 from PNENs G1/G2,including tumor size,shape,margin,heterogeneity,intratumoral blood vessels,vascular invasion,enhancement pattern in both contrast phases,enhancement degree in both phases,tumor-to-pancreas contrast ratio in both phases,common bile duct dilatation,lymph node metastases,and liver ***-defined tumor margin was an independent predictor for PNENs G3 with the highest area under the curve(AUC)of 0.906 in the multivariable logistic regression and receiver operating characteristic curve *** portal enhancement ratio(PER)was shown the highest AUC of 0.855 in terms of quantitative *** data suggest that the traditional contrastenhanced CT still plays a vital role in differentiation of tumor grades and heterogeneity analysis prior to treatment.

关键词： pancreatic neuroendocrine neoplasm computed tomography tumor grade heterogeneity analysis

Quantitative trait locus mapping of yield and plant height in autotetraploid alfalfa(Medicago sativa L.)

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The Crop Journal 2020年第5期8卷 812-818页

作者： Fei He Ruicai Long Tiejun Zhang Fan Zhang Zhen Wang Xijiang Yang Xueqian Jiang Changfu Yang Xuxin Zhi mingna li Longxi Yu Junmei Kang Qingchuan Yang Institute of Animal Science Chinese Academy of Agricultural SciencesBeijing 100193China Plant and Germplasm Introduction and Testing Research United States Department of Agriculture-Agricultural Research ServiceProsserWA 99350USA

Alfalfa(Medicago sativa L.)is the most widely grown forage legume crop *** and plant height are important agronomic traits influenced by genetic and environmental *** objective of this study was to identify quantitative trait loci(QTL)and molecular markers associated with alfalfa yield and plant *** understand the genetic basis of these traits,a full-sib F1 population composed of 392 individuals was developed by crossing a low-yielding precocious alfalfa genotype(male parent)with a high-yielding latematuring alfalfa cultivar(female parent).The linkage maps were constructed with 3818 single-nucleotide polymorphism(SNP)markers on 64 linkage *** for yield and plant height were mapped using phenotypic data for three *** QTL associated with yield and plant height were identified on chromosomes 1 to *** QTL explained more than 10%of phenotypic variation,representing major loci controlling yield and plant *** locus on chromosome 1 controlling yield traits had not been identified in previous *** QTL co-located with other QTL(qyield-1 and qheight-7,qheight-5 and qyield-4,qheight-6,and qyield-6).With further validation,the markers closely linked with these QTL may be used for marker-assisted selection in breeding new alfalfa varieties with high yield.

关键词： Alfalfa Yield Plant height Genetic linkage map Quantitative trait loci

Genome Assembly of Alfalfa Cultivar Zhongmu-4 and Identification of SNPs Associated with Agronomic Traits

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Genomics, Proteomics & Bioinformatics 2022年第1期20卷 14-28页

作者： Ruicai Long Fan Zhang Zhiwu Zhang mingna li lin Chen Xue Wang Wenwen liu Tiejun Zhang Long-Xi Yu Fei He Xueqian Jiang Xijiang Yang Changfu Yang Zhen Wang Junmei Kang Qingchuan Yang Institute of Animal Sciences Chinese Academy of Agricultural SciencesBeijing 100193China Department of Crop and Soil Sciences Washington State UniversityPullmanWA 99163USA School of Grassland Science Beijing Forestry UniversityBeijing 100083China United States Department of Agriculture-Agricultural Research Service Plant and Germplasm Introduction and Testing ResearchProsserWA 99350USA

Alfalfa(Medicago sativa L.)is the most important legume forage crop worldwide with high nutritional value and *** a long time,the breeding of alfalfa was hampered by lacking reliable information on the autotetraploid genome and molecular markers linked to important agronomic *** herein reported the de novo assembly of the allele-aware chromosome-level genome of Zhongmu-4,a cultivar widely cultivated in China,and a comprehensive database of genomic variations based on resequencing of 220 *** 2.74 Gb contigs(N50 of 2.06 Mb),accounting for 88.39%of the estimated genome,were assembled,and 2.56 Gb contigs were anchored to 32 pseudo-chromosomes.A total of 34,922 allelic genes were identified from the allele-aware *** observed the expansion of gene families,especially those related to the nitrogen metabolism,and the increase of repetitive elements including transposable elements,which probably resulted in the increase of Zhongmu-4 genome compared with Medicago *** structure analysis revealed that the accessions from Asia and South America had relatively lower genetic diversity than those from Europe,suggesting that geography may influence alfalfa genetic divergence during local ***-wide association studies identified 101 single nucleotide polymorphisms(SNPs)associated with 27 agronomic *** candidate genes were predicted to be correlated with fall dormancy and salt *** believe that the alleleaware chromosome-level genome sequence of Zhongmu-4 combined with the resequencing data of the diverse alfalfa germplasms will facilitate genetic research and genomics-assisted breeding in variety improvement of alfalfa.

关键词： Alfalfa Autotetraploid Genome assembly Resequencing Genome-wide association study

Evaluating reverse docking on general and selective inhibitors: a case study about glide

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Journal of Chinese Pharmaceutical Sciences 2020年第10期29卷 679-688页

作者： mingna li Xing Wu liangren Zhang Zhenming liu State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking University Health Science CenterBeijing 100191China Hengdian Group Holdings limited Hangzhou 310007China

As a powerful tool for target prediction,reverse docking remains largely *** objective evaluation of reverse docking software can help us know better about the strength and weakness of these tools,hence guiding us in target *** the present study,we evaluated the target prediction power of Glide(SP)against general inhibitors and selective *** results showed that the scoring tendency could be different for each ligand,and overall scoring sampling was necessary for a better understanding of the docking score for a certain protein-ligand ***,the input conformation of the binding pocket could affect the docking ***(SP)showed a preferable performance on the target prediction of the general ***,the accuracy of the target prediction of the selective inhibitors was relatively low,indicating that Glide(SP)might not be capable for this *** case study about COVID-19 proved that coagulation factor Xa might be a potential target of ***,we recommend the further development of reverse docking tools and rectification of inter-target scoring bias.

关键词： Reverse docking Target prediction Software evaluation

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教育对流动人口工资收入的影响研究

人口学刊 2021年第6期43卷 28-40页

作者：李铭娜回莹长春大学文学院吉林长春130012 廊坊师范学院经济与管理学院河北廊坊065000

教育关系一个国家和民族的未来,是事关国家兴衰的最根本事业。接受良好教育已经成为人们生存发展的第一需要和终身受益的财富。针对“读书无用”和“学历不值钱”等社会言论,本文从个体工资收入的角度深入研究教育的作用,归纳出“教育-... 详细信息

教育关系一个国家和民族的未来,是事关国家兴衰的最根本事业。接受良好教育已经成为人们生存发展的第一需要和终身受益的财富。针对“读书无用”和“学历不值钱”等社会言论,本文从个体工资收入的角度深入研究教育的作用,归纳出“教育-人力资本-劳动生产率-工资收入”的因果关系链,进而利用2017年中国流动人口调查数据实证研究教育对个体工资收入的影响。研究发现:教育、就业类型和工资收入存在紧密的相关关系,研究生学历的个体从事脑力劳动的可能性更大,获得高水平收入的可能性也更大;教育能够显著正向影响个体的工资收入水平,相对于高中及以下学历的个体,接受大专大学教育和研究生教育的个体工资收入溢价幅度达到22.7%和63.5%,研究生教育对工资收入的提升作用更加明显;体力劳动者、混合劳动者和脑力劳动者的教育回报率不同,教育对体力劳动者工资收入的提升作用最大。结合我国实际,提出鼓励个体追求卓越,形成专业型人力资本;扩大政府对高等教育尤其是研究生教育的投入力度;加快形成崇尚读书和奖励专门研究的社会氛围等政策建议。

关键词：教育流动人口人力资本就业类型工资收入

Evolutionary genomics of climatic adaptation and resilience to climate change in alfalfa

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Molecular Plant 2024年第6期17卷 867-883页

作者： Fan Zhang Ruicai Long Zhiyao Ma Hua Xiao Xiaodong Xu Zhongjie liu Chunxue Wei Yiwen Wang Yanling Peng Xuanwen Yang Xiaoya Shi Shuo Cao mingna li Ming Xu Fei He Xueqian Jiang Tiejun Zhang Zhen Wang Xianran li Long-Xi Yu Junmei Kang Zhiwu Zhang Yongfeng Zhou Qingchuan Yang Institute of Animal Science Chinese Academy of Agricultural SciencesBeijing 100193China National Key Laboratory of Tropical Crop Breeding Shenzhen BranchGuangdong Laboratory of Lingnan Modern AgricultureKey Laboratory of Synthetic BiologyMinistry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China School of Grassland Science Beijing Forestry UniversityBeijing 100083China Department of Agronomy and Horticulture University of Nebraska-LincolnLincolnNE 68583USA Department of Crop and Soil Sciences Washington State UniversityPullmanWA 99163USA U.S.Department of Agriculture-Agricultural Research Service Plant Germplasm Introduction and Testing ResearchProsserWA 99350USA National Key Laboratory of Tropical Crop Breeding Tropical Crops Genetic Resources InstituteChinese Academy of Tropical Agricultural SciencesHaikou 571101China

Given the escalating impact of climate change on agriculture and food security,gaining insights into the evolutionary dynamics of climatic adaptation and uncovering climate-adapted variation can empower the breeding of climate-resilient crops to face future climate ***(Medicago sativa ***),the queen of forages,shows remarkable adaptability across diverse global environments,making it an excellent model for investigating species responses to climate *** this study,we performed population genomic analyses using genome resequencing data from 702 accessions of 24 Medicago species to unravel alfalfa’s climatic adaptation and genetic susceptibility to future climate *** found that interspecific genetic exchange has contributed to the gene pool of alfalfa,particularly enriching defense and stress-response *** introgression between *** ***(***)and alfalfa not only aids alfalfa’s climatic adaptation but also introduces genetic burden.A total of 1671 genes were associated with climatic adaptation,and 5.7%of them were introgressions from *** integrating climate-associated variants and climate data,we identified populations that are vulnerable to future climate change,particularly in higher latitudes of the Northern *** findings serve as a clarion call for targeted conservation initiatives and breeding *** also identified preadaptive populations that demonstrate heightened resilience to climate fluctuations,illuminating a pathway for future breeding ***,this study enhances our understanding about the local adaptation mechanisms of alfalfa and facilitates the breeding of climate-resilient alfalfa cultivars,contributing to effective agricultural strategies for facing future climate change.

关键词： Medicago local adaptation population genetics adaptive introgression genetic vulnerability alfalfa breeding

The growth and development rule and histological observation of ovaries in domestic chicken

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The growth and development rule and histological observation...

中国畜牧兽医学会动物繁殖学分会第二十次学术研讨会

作者： Jos Dorian Lawson Mfoundou Guo Yajun liu Miaomiao Ran Xinrong Fu Dehai li mingna Wang Xinrong College of Animal Science and Technology Gansu Agricultural University

(Objective) The present study was conducted with the goal of investigating themorphological and histological changes in ovaries and their components during the growth and development in domestic chicken at different age stages,and exploring the developmental changes and rule in ovaries.(Method) Fifty healthy female chicks(Hy-line Brown) aged 1 and 3-weeks,1,2,and 3-months obtained from a local poultry farm which specializes in breeding chicks were used in this ***,the chicks were weighted and slaughtered,then dissected at the lower abdominal portion to the thoracic cavity to observe and record the growth and location of ovaries in the ***,the ovaries were carefully harvested,their weight and measurements(length,width,and thickness) were ***,the ovaries were immediately placed in AAF solution for fixation,processed and immersed in wax blocks,then cut into 5 to 7 μm sections,stained for microscopic observations using hematoxylin and eosin(H.E.) techniques.(Result)Results found that the ovaries were located high in the abdominal cavity,attached to the backbone on either side of the dorsal aorta,and the left organ was larger and longer than the right *** left ovary showed a deep yellow creamy tissue mass at its anterior part and displayed various shapes and sizes during development,showing a smooth aspect(one-week-old to three-week) which evolved and became more vascularized,making it look more uneven and completely lost its smooth aspect(three-month-old).The remaining right ovary was situated on top of the right kidney closely attached to the anterior part of the left ovary and displayed a deep yellow creamy tissue mass similar to that of the left ovary which reflected its key remaining characteristic and did not seem to decline but continued to develop up to three months *** tohistological observation,the left ovary(one-week-old) exhibited a thick cortex that spread considerably and grew thicker as follicles formed.T

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撰写综述性科技论文常见问题

黑龙江工程学院学报 2017年第5期31卷 78-80页

作者：李铭娜黑龙江工程学院学术理论研究部黑龙江哈尔滨150050

在编辑工作中,经常收到一些综述性论文,有的文章论述篇幅过长、主题不明确,有的文章将理论、观点、方法简单摘录,缺乏精炼的语言将其概括出来,论文选题出现的问题究其原因:写作经验不足、文献查阅量少、归纳总结能力不够、知识深度掌握... 详细信息

在编辑工作中,经常收到一些综述性论文,有的文章论述篇幅过长、主题不明确,有的文章将理论、观点、方法简单摘录,缺乏精炼的语言将其概括出来,论文选题出现的问题究其原因:写作经验不足、文献查阅量少、归纳总结能力不够、知识深度掌握不够,这些都影响科技论文观点提出、事实的论述。针对这些问题,提出文章选题要适当,不要盲目过大;查阅大量的文献资料做好摘抄和记录;学会分析总结自己见解,预测未来该领域的发展趋势,为研究选题和开题奠定良好的基础。

关键词：综述性论文文题不符主题不明确