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Genome-wide identification and expression analysis of NtbHLH gene family in tobacco(Nicotiana tabacum)and the role of NtbHLH86 in drought adaptation

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Plant Diversity 2021年第6期43卷 510-522页

作者： Ge Bai Da-Hai Yang Peijian Chao Heng Yao mingliang fei Yihan Zhang Xuejun Chen Bingguang Xiao Feng Li Zhen-Yu Wang Jun Yang He Xie Tobacco Breeding and Biotechnology Research Center Yunnan Academy of Tobacco Agricultural SciencesKunmingYunnanChina Key Laboratory of Tobacco Biotechnological Breeding KunmingYunnanChina National Tobacco Genetic Engineering Research Center KunmingYunnanChina National Tobacco Gene Research Centre Zhengzhou Tobacco Research InstituteZhengzhouHenanChina Institute ofBioengineering Guangdong Academy of SciencesGuangzhouGuangdong510316China

The bHLH transcription factors play pivotal roles in plant growth and development,production of secondary metabolites and responses to various environmental *** the bHLH genes have been well studied in model plant species,a comprehensive investigation of the bHLH genes is required for tobacco with newly obtained high-quality *** the present study,a total of 309 NtbHLH genes were identified and can be divided into 23 *** conserved amino acids which are essential for their function were predicted for the NtbHLH ***,the NtbHLH genes were conserved during evolution through analyzing the gene structures and conserved motifs.A total of 265 NtbHLH genes were localized in the 24 tobacco chromosomes while the remained 44 NtbHLH genes were mapped to the scaffolds due to the complexity of tobacco ***,transcripts of NtbHLH genes were obviously tissue-specific expressed from the gene-chip data from 23 tobacco tissues,and expressions of 20 random selected NtbHLH genes were further confirmed by quantitative real-time PCR,indicating their potential functions in the plant growth and ***,overexpressed NtbHLH86 gene confers improve drought tolerance in tobacco indicating that it might be involved in the regulation of drought ***,our findings here provide a valuable information on the characterization of NtbHLH genes and further investigation of their functions in tobacco.

关键词： bHLH gene family Development Genome-wide analysis Characterization

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A Dual-Promoter Gene Orchestrates the Sucrose-Coordinated Synthesis of Starch and Fructan in Barley

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Molecular Plant 2017年第12期10卷 1556-1570页

作者： Yunkai Jin mingliang fei Sara Rosenquist Lu Jin Suresh Gohil Corine Sandstrom Helena Olsson Cecilia Persson Anna-Stina Hoglund Gunnel Fransson Ying Ruan Per Aman Christer Jansson Chunlin Liu Roger Andersson Chuanxin Sun Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization Hunan Agricultural University Changsha 410128 China Department of Plant Biology Uppsala BioCenter Linnean Centre for Plant Biology Swedish University of Agricultural Sciences P.O. Box 7080 75007 UppsalaSweden Key Laboratory of Education Department of Hunan Province on Plant Genetics and Molecular Biology College of Bioscience and BiotechnologyHunan Agricultural University Changsha 410128 China Department of Chemistry and Biotechnology Uppsala BioCenter Swedish University of Agrioultural Scienoes P.O. Box 7015 75007 Uppsala Sweden The Swedish NMR Centre at University of Gothenburg Box 465 405 30 Gothenburg Sweden Department of Molecular Sciences Uppsala BioCenter Swedish University of Agricultural Sciences P.O. Box 7051 750 07 Uppsala Sweden The Environmental Molecular Sciences Laboratory (EMSL) Pacific Northwest National Laboratory P.O. Box 999 K8-93 Richland WA 99352 USA These authors contributed equally to this article.

Sequential carbohydrate synthesis is important for plant survival because it guarantees energy supplies for growth and development during plant ontogeny and reproduction. Starch and fructan are two important carbohydrates in many flowering plants and in human diets. Understanding this coordinated starch and fructan synthesis and unraveling how plants allocate photosynthates and prioritize different carbohydrate synthesis for survival could lead to improvements to cereals in agriculture for the purposes of greater food security and production quality. Here, we report a system from a single gene in barley employing two alternative promoters, one intronic/exonic, to generate two sequence-overlapping but functionally opposing transcription factors, in sensing sucrose, potentially via sucrose/gluco lose 6-phosphate signaling. The system employs an autoregulatory mechanism in perceiving a sucrose- controlled trans activity on one promoter and orchestrating the coordinated starch and fructan synthesis by competitive transcription factor binding on the other promoter. As a case in point for the physiological roles of the system, we have demonstrated that this multitasking system can be exploited in breeding barley with tailored amounts of fructan to produce healthy food ingredients. The identification of an intron/exon-spanning promoter in a hosting gene, resulting in proteins with distinct functions, adds to the complexity of plant genomes.

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烟草SnRK2基因家族的克隆及表达分析

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分子植物育种 2024年第12期22卷 3877-3884页

作者：白戈杨大海费明亮谢贺云南省烟草农业科学研究院烟草生物技术重点实验室国家烟草基因工程研究中心昆明650021

农作物生产过程中会面临多种非生物胁迫的影响,会极大地影响作物的产量以及品质。非生物胁迫形式多种多样,干旱胁迫是其中一种主要形式。烟草是重要的经济作物,主要在中国西南地区种植,但经常会遭受极端干旱胁迫。Sn RK2基因家族参与植... 详细信息

农作物生产过程中会面临多种非生物胁迫的影响,会极大地影响作物的产量以及品质。非生物胁迫形式多种多样,干旱胁迫是其中一种主要形式。烟草是重要的经济作物,主要在中国西南地区种植,但经常会遭受极端干旱胁迫。Sn RK2基因家族参与植物抗旱过程,能够受逆境胁迫诱导表达,这种表达是SnRK2基因家族功能的重要体现。为了进一步研究烟草SnRK2家族基因的功能,本研究克隆了烟草SnRK2基因家族GroupⅠ的基因,并检测了烟草SnRK2基因家族的组织表达特异性及诱导表达特异性。结果表明,烟草SnRK2基因中Ntab0922420基因的表达量最高,同时烟草SnRK2基因家族成员更容易在茎与叶中表达。烟草经过低温、盐、干旱胁迫以及ABA处理后,部分SnRK2基因成员表现出受胁迫诱导,其中ID为Ntab0894060的基因受干旱显著诱导。这些工作将为研究烟草SnRK2基因家族功能鉴定基础,并为培育抗逆烟草品种提供遗传资源。

关键词：烟草 SnRK2 表达分析

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基于iTRAQ-PRM技术筛选不同类型土壤烤烟根系差异表达蛋白

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中国烟草学报 2022年第1期28卷 86-97页

作者：杨睿查文菊陈颐何承刚王涛何聪莲费明亮云南省烟草农业科学研究院云南省昆明市650031 云南农业大学云南省昆明市650201 云南省烟草公司曲靖市公司云南省曲靖市671000

【目的】从蛋白质水平揭示烤烟根系对不同类型土壤的响应差异。【方法】以烤烟品种K326为材料,选取石灰岩土(沙壤土)、水稻土和红壤土,测定3种类型土壤烤烟根系活力、硝酸还原酶(NR)、谷氨酰胺合成酶(GS)和天冬氨酸合成酶(AS)活性;利用i... 详细信息

【目的】从蛋白质水平揭示烤烟根系对不同类型土壤的响应差异。【方法】以烤烟品种K326为材料,选取石灰岩土(沙壤土)、水稻土和红壤土,测定3种类型土壤烤烟根系活力、硝酸还原酶(NR)、谷氨酰胺合成酶(GS)和天冬氨酸合成酶(AS)活性;利用iTRAQ技术鉴定3种类型土壤烤烟根系差异表达蛋白,对所获得的差异蛋白进行生物信息学分析,从中选择16个蛋白进行平行反应监测(PRM)验证。【结果】(1)红壤土的烤烟根系活力、NR、GS和AS活性均高于沙壤土和水稻土;(2)从水稻土vs沙壤土、沙壤土vs红壤土、水稻土vs红壤土3组对比中分别鉴定出699、650、569个烤烟根系差异表达蛋白,其中上调/下调蛋白分别为412/287、291/359和323/246个;(3)差异蛋白质主要有催化、结合、转运活性等功能;(4)差异蛋白功能富集于次生代谢产物的生物合成、代谢途径和苯丙烷类生物合成等代谢途径;(5)红壤土烤烟根系与抗逆性、碳水化合物和能量代谢、蛋白质合成等相关的差异蛋白表达均高于沙壤土和水稻土。【结论】利用iTRAQ标记技术,结合PRM验证技术筛选出不同类型土壤烤烟根系差异表达蛋白,为获取响应关键蛋白奠定基础。

关键词： iTRAQ 烟草根系土壤类型氮代谢蛋白质组学

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Optimizing the key parameter to accelerate the recovery of AMOC under a rapid increase of greenhouse gas forcing

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Atmospheric and Oceanic Science Letters 2025年第1期18卷 39-45页

作者： Haolan Ren fei Zheng Tingwei Cao Qiang Wang International Center for Climate and Environment Science(ICCES) Institute of Atmospheric PhysicsChinese Academy of SciencesBeijingChina University of Chinese Academy of Sciences BeijingChina College of Oceanography Hohai UniversityNanjingChina CAS Key Laboratory of Ocean Circulation and Waves Institute of OceanologyChinese Academy of Sciences and Pilot National Laboratory for Marine Science and Technology(Qingdao)QingdaoChina

Atlantic Meridional Overturning Circulation(AMOC)plays a central role in long-term climate variations through its heat and freshwater transports,which can collapse under a rapid increase of greenhouse gas forcing in climate *** studies have suggested that the deviation of model parameters is one of the major factors in inducing inaccurate AMOC *** this work,with a low-resolution earth system model,the authors try to explore whether a reasonable adjustment of the key model parameter can help to re-establish the AMOC after its *** a new optimization strategy,the extra freshwater flux(FWF)parameter is determined to be the dominant one affecting the AMOC’s *** traditional ensemble optimal interpolation(EnOI)data assimilation and new machine learning methods are adopted to optimize the FWF parameter in an abrupt 4×CO_(2) forcing experiment to improve the adaptability of model parameters and accelerate the recovery of *** results show that,under an abrupt 4×CO_(2) forcing in millennial simulations,the AMOC will first collapse and then re-establish by the default FWF parameter ***,during the parameter adjustment process,the saltier and colder sea water over the North Atlantic region are the dominant factors in usefully improving the adaptability of the FWF parameter and accelerating the recovery of AMOC,according to their physical relationship with FWF on the interdecadal timescale.

关键词： Recovery of AMOC 4×CO_(2)forcing Key parameter Parameter estimation Data assimilation Machine learning

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Epigenetic regulation of the inflammatory response in stroke

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Neural Regeneration Research 2025年第11期20卷 3045-3062页

作者： Jingyi Liang fei Yang Zixiao Li Qian Li School of Basic Medical Sciences Capital Medical UniversityBeijingChina Department of Neurobiology School of Basic Medical SciencesCapital Medical UniversityBeijingChina Laboratory for Clinical Medicine Beijing Key Laboratory of Neural Regeneration and RepairCapital Medical UniversityBeijingChina Department of Neurology Beijing Tiantan HospitalCapital Medical UniversityBeijingChina China National Clinical Research Center for Neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijingChina National Center for Healthcare Quality Management in Neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Chinese Institute for Brain Research BeijingChina Research Unit of Artificial Intelligence in Cerebrovascular Disease Chinese Academy of Medical SciencesBeijingChina Beijing Engineering Research Center of Digital Healthcare for Neurological Diseases BeijingChina Department of Biochemistry and Molecular Biology School of Basic Medical SciencesCapital Medical UniversityBeijingChina Beijing Key Laboratory of Cancer Invasion and Metastasis Research Capital Medical UniversityBeijingChina

Stroke is classified as ischemic or hemorrhagic,and there are few effective treatments for either *** mechanisms play a critical role in secondary brain injury following a stroke,which manifests as cytokine release,blood–brain barrier disruption,neuronal cell death,and ultimately behavioral *** the inflammatory response has been shown to mitigate this cascade of events in experimental stroke ***,in clinical trials of anti-inflammatory agents,longterm immunosuppression has not demonstrated significant clinical benefits for *** may be attributable to the dichotomous roles of inflammation in both tissue injury and repair,as well as the complex pathophysiologic inflammatory processes in *** acute harmful inflammatory responses or inducing a phenotypic shift from a pro-inflammatory to an anti-inflammatory state at specific time points after a stroke are alternative and promising therapeutic *** agents that can modulate inflammation requires a detailed understanding of the inflammatory processes of ***,epigenetic reprogramming plays a crucial role in modulating post-stroke inflammation and can potentially be exploited for stroke *** this review,we summarize current findings on the epigenetic regulation of the inflammatory response in stroke,focusing on key signaling pathways including nuclear factor-kappa B,Janus kinase/signal transducer and activator of transcription,and mitogen-activated protein kinase as well as inflammasome *** also discuss promising molecular targets for stroke *** evidence to date indicates that therapeutic targeting of the epigenetic regulation of inflammation can shift the balance from inflammation-induced tissue injury to repair following stroke,leading to improved post-stroke outcomes.

关键词： DNA methylation histone modification intracerebral hemorrhage ischemic stroke neuroinflammation neuroprotection non-coding RNA RNA methylation subarachnoid hemorrhage treatment

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Co-production of hydrogen, oxygen, and electricity via an integrated solar-driven system with decoupled water electrolyzer and Na-Zn ion battery

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Journal of Energy Chemistry 2025年第1期100卷 621-627页

作者： fei Lv Longjie Liu Jiazhe Wu Pengfei Wang Lixia Pan Dengwei Jing Yubin Chen International Research Center for Renewable Energy State Key Laboratory of Multiphase Flow in Power Engineering Xi'an Jiaotong University

Combining water electrolysis and rechargeable battery technologies into a single system holds great promise for the co-production of hydrogen （H2） and ***,the design and development of such systems is still in its ***,an integrated hydrogen-oxygen （O2）-electricity co-production system featuring a bipolar membrane-assisted decoupled electrolyzer and a Na-Zn ion battery was established with sodium nickelhexacyanoferrate （NaNiHCF） and Zn2+/Zn as dual redox *** decoupled electrolyzer enables to produce H2and O2in different time and space with almost 100%Faradaic efficiency at 100 mA ***,the charged NaNiHCF and Zn electrodes after the electrolysis processes formed a Na-Zn ion battery,which can generate electricity with an average cell voltage of 1.75 V at 10 m A *** connecting Si photovoltaics with the modular electrochemical device,a well-matched solar driven system was built to convert the intermittent solar energy into hydrogen and electric energy with a solar to hydrogen-electricity efficiency of 16.7%,demonstrating the flexible storage and conversion of renewables.

关键词： Hydrogen Electricity Decoupled water electrolyzer Na-Zn ion battery Solar energy

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阿南德氏链霉菌FXJ-25的鉴定及对烟草靶斑病的防效

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中国烟草科学 2024年第2期45卷 56-63页

作者：苏家恩杨志娟董天学赵英佑王显跃费明亮陈颐钟杰云南省烟草公司大理市公司云南大理671000 云南省烟草公司曲靖市公司云南曲靖655000 云南省烟草农业科学研究院昆明650031 湖南农业大学植物保护学院长沙410128

为筛选烟草靶斑病菌拮抗菌,丰富烟草靶斑病生防资源,采用稀释涂布法和平板对峙法进行放线菌分离,筛选得到一株具有明显拮抗效果的菌株FXJ-25。基于培养特性、生理生化特性及16S rDNA基因序列分析相结合的方法对该菌株进行鉴定,分析了其... 详细信息

为筛选烟草靶斑病菌拮抗菌,丰富烟草靶斑病生防资源,采用稀释涂布法和平板对峙法进行放线菌分离,筛选得到一株具有明显拮抗效果的菌株FXJ-25。基于培养特性、生理生化特性及16S rDNA基因序列分析相结合的方法对该菌株进行鉴定,分析了其诱导烟草抗性的能力,并通过盆栽试验对其防效进行了研究。结果表明,拮抗菌FXJ-25对烟草靶斑病菌的抑菌率为74.14%,可引起菌丝畸形,经鉴定该菌株为阿南德氏链霉菌(Streptomyces anandii)。FXJ-25具有广谱抗真菌活性,对瓜类蔓枯病菌(Stagonosporopsis cucurbitacearum)、烟草喀斯特炭疽菌(Colletotrichum karstii)和小麦赤霉病菌(Fusarium graminearum)等15种植物病原真菌均具有较好的拮抗作用,抑菌率在53.43%~100%之间。菌株发酵液还可显著提高烟苗苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)和过氧化氢酶(CAT)等防御相关酶的活性。盆栽防效中,菌株FXJ-25活菌液(108孢子/mL)对烟草靶斑病具有78.81%的防效。因此,FXJ-25可作为烟草靶斑病的生防材料进一步开发利用。

关键词：阿南德氏链霉菌生物防治烟草靶斑病抑菌谱

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Interaction of major facilitator superfamily domain containing 2A with the blood-brain barrier

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Neural Regeneration Research 2025年第8期20卷 2133-2152页

作者： Yilun Ma Taiwei Dong fei Luan Juanjuan Yang Feng Miao Peifeng Wei College of Pharmacy and First Clinical Medical College Shaanxi University of Chinese MedicineXianyangShaanxi ProvinceChina National Drug Clinical Trial Agency The Second Affiliated Hospital of Shaanxi University of Chinese Medicine/Xixian New District Central HospitalXi′anShaanxi ProvinceChina

The functional and structural integrity of the blood-brain barrier is crucial in maintaining homeostasis in the brain microenvironment;however,the molecular mechanisms underlying the formation and function of the blood-brain barrier remain poorly *** major facilitator superfamily domain containing 2A has been identified as a key regulator of blood-brain barrier *** plays a critical role in promoting and maintaining the formation and functional stability of the blood-brain barrier,in addition to the transport of lipids,such as docosahexaenoic acid,across the blood-brain ***,an increasing number of studies have suggested that major facilitator superfamily domain containing 2A is involved in the molecular mechanisms of blood-brain barrier dysfunction in a variety of neurological diseases;however,little is known regarding the mechanisms by which major facilitator superfamily domain containing 2A affects the blood-brain *** paper provides a comprehensive and systematic review of the close relationship between major facilitator superfamily domain containing 2A proteins and the blood-brain barrier,including their basic structures and functions,cross-linking between major facilitator superfamily domain containing 2A and the blood-brain barrier,and the in-depth studies on lipid transport and the regulation of blood-brain barrier *** comprehensive systematic review contributes to an in-depth understanding of the important role of major facilitator superfamily domain containing 2A proteins in maintaining the structure and function of the blood-brain barrier and the research progress to *** will not only help to elucidate the pathogenesis of neurological diseases,improve the accuracy of laboratory diagnosis,and optimize clinical treatment strategies,but it may also play an important role in prognostic *** addition,the effects of major facilitator superfamily domain containing 2A on blood-brain barrier lea

关键词： blood-brain barrier(BBB) caveolin-1 central nervous system docosahexaenoic acid endothelial cells lysophosphatidylcholine major facilitator superfamily domain containing 2A(MFSD2A) transcytosis

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Secure Power and Subcarrier Auction in Uplink FullDuplex Cellular Networks

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China Communications 2015年第S1期12卷 157-165页

作者： LI mingliang GUO Yunfei HUANG Kaizhi GUO fei National Digital Switching System Engineering & Technological R&D Center

We consider a cellular network with a full-duplex base station, multiple uplink users and an eavesdropper. The full-duplex base station transmits jamming signals to degrade the eavesdropper channel when receiving secure multi-user signals. To maximize the secrecy rate of uplink communications, we propose a distributed ascending-clock auction(ACA) algorithm to allocate subcarriers and jamming power. Specifically, the impact of the self-interference of the full-duplex base station on the secrecy rate is considered. The proposed algorithm consists of two parts. Firstly, subcarriers and the jamming power are respectively priced by the base station. Secondly, users select the subcarrier and the jamming power based on the price. Moreover, the convergence of the proposed auction algorithm is mathematically proved. Simulation results show that the proposed auction algorithm is more beneficial to improve the uplink secrecy performance compared to traditional auction algorithms.

关键词： uplink auction physical layer security full-duplex self-interference

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