Genome-wide identification and expression analysis of NtbHLH gene family in tobacco(Nicotiana tabacum)and the role of NtbHLH86 in drought adaptation

作     者：Ge Bai Da-Hai Yang Peijian Chao Heng Yao MingLiang Fei Yihan Zhang Xuejun Chen Bingguang Xiao Feng Li Zhen-Yu Wang Jun Yang He Xie Ge Bai;Da-Hai Yang;Peijian Chao;Heng Yao;MingLiang Fei;Yihan Zhang;Xuejun Chen;Bingguang Xiao;Feng Li;Zhen-Yu Wang;Jun Yang;He Xie

作者机构：Tobacco Breeding and Biotechnology Research CenterYunnan Academy of Tobacco Agricultural SciencesKunmingYunnanChina Key Laboratory of Tobacco Biotechnological BreedingKunmingYunnanChina National Tobacco Genetic Engineering Research CenterKunmingYunnanChina National Tobacco Gene Research CentreZhengzhou Tobacco Research InstituteZhengzhouHenanChina Institute ofBioengineeringGuangdong Academy of SciencesGuangzhouGuangdong510316China 

出 版 物：《Plant Diversity》 (植物多样性（英文版）)

年 卷 期：2021年第43卷第6期

页      面：510-522页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：funded by the National Natural Science Foundation of China(grant number 31760072 to G.Bai,and grant number 31860413 to H.Xie) Yunnan Applied Basic Research Project(grant number 202001AT070010 to G.Bai) the Yunnan Academy of Tobacco Agricultural Sciences(grant numbers YNTC-2016YN22 and CNTC-110202001025(JY08)to H.Xie,YNTC-2016YN24,YNTC-2015YN02,YNTC-2018530000241002,and YNTC-2019530000241003 to D.-H.Yang) 

主　　题：bHLH gene family Development Genome-wide analysis Characterization 

摘      要：The bHLH transcription factors play pivotal roles in plant growth and development,production of secondary metabolites and responses to various environmental *** the bHLH genes have been well studied in model plant species,a comprehensive investigation of the bHLH genes is required for tobacco with newly obtained high-quality *** the present study,a total of 309 NtbHLH genes were identified and can be divided into 23 *** conserved amino acids which are essential for their function were predicted for the NtbHLH ***,the NtbHLH genes were conserved during evolution through analyzing the gene structures and conserved motifs.A total of 265 NtbHLH genes were localized in the 24 tobacco chromosomes while the remained 44 NtbHLH genes were mapped to the scaffolds due to the complexity of tobacco ***,transcripts of NtbHLH genes were obviously tissue-specific expressed from the gene-chip data from 23 tobacco tissues,and expressions of 20 random selected NtbHLH genes were further confirmed by quantitative real-time PCR,indicating their potential functions in the plant growth and ***,overexpressed NtbHLH86 gene confers improve drought tolerance in tobacco indicating that it might be involved in the regulation of drought ***,our findings here provide a valuable information on the characterization of NtbHLH genes and further investigation of their functions in tobacco.