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Core clock component MtLUX controls shoot architecture through repression of MtTB1/MtTCP1A in Medicago truncatula

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The Crop Journal 2023年第3期11卷 723-732页

作者： Liping Wang Anqi Zhou Lulu Wang Jing Li mingkang yang Tingting Duan Jian Jin Liang Chen Liangfa Ge Wei Huang State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Life SciencesSouth China Agricultural UniversityGuangzhou 510642GuangdongChina Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510642GuangdongChina Department of Grassland Science College of Forestry and Landscape ArchitectureSouth China Agricultural UniversityGuangzhou 510642GuangdongChina Guangdong Engineering Research Center for Grassland Science Guangzhou 510642GuangdongChina Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm South China Agricultural UniversityGuangzhou 510642GuangdongChina Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms College of Life SciencesSouth China Agricultural UniversityGuangzhou 510642GuangdongChina State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Life Science and TechnologyGuangxi UniversityNanning 530004GuangxiChina

Plants are capable of regulating their shoot architecture in response to diverse internal and external environments.The circadian clock is an adaptive mechanism that integrates information from internal and ambient conditions to help plants cope with recurring environmental fluctuations.Despite the current understanding of plant circadian clock and genetic framework underlying plant shoot architecture,the intricate connection between these two adaptive mechanisms remains largely unclear.In this study,we elucidated how the core clock gene LUX ARRHYTHMO(LUX)regulates shoot architecture in the model legume plant Medicago truncatula.We show that mtlux mutant displays increased main stem height,reduced lateral shoot length,and decreased the number of lateral branches and biomass yield.Gene expression analysis revealed that Mt LUX regulated shoot architecture by repressing the expression of strigolactone receptor MtD14 and MtTB1/MtTCP1A,a TCP gene that functions centrally in modulating shoot architecture.In vivo and in vitro experiments showed that Mt LUX directly binds to a cis-element in the promoter of MtTB1/MtTCP1A,suggesting that Mt LUX regulates branching by rhythmically suppressing MtTB1/MtTCP1A.This work demonstrates the regulatory effect of the circadian clock on shoot architecture,offering a new understanding underlying the genetic basis towards the flexibility of plant shoot architecture.

关键词： Medicago truncatula Circadian clock MtLUX Shoot architecture MtTB1/MtTCP1A

Circadian rhythms driving a fast-paced root clock implicate species-specific regulation in Medicago truncatula

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Journal of Integrative Plant Biology 2021年第8期63卷 1537-1554页

作者： Liping Wang Anqi Zhou Jing Li mingkang yang Fan Bu Liangfa Ge Liang Chen Wei Huang State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural UniversityGuangzhou510642 China College of Life Sciences South China Agricultural UniversityGuangzhou510642 China Department of Grassland Science College of Forestry and Landscape ArchitectureSouth China Agricultural UniversityGuangzhou510642 China Guangdong Engineering Research Center for Grassland Science Guangzhou510642 China Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm South China Agricultural UniversityGuangzhou510642 China Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms College of Life SciencesSouth China Agricultural UniversityGuangzhou510642 China

Plants have a hierarchical circadian structure comprising multiple tissue-specific oscillators that operate at different speeds and regulate the expression of distinct sets of genes in different organs.However,the identity of the genes differentially regulated by the circadian clock in different organs,such as roots,and how their oscillations create functional specialization remain unclear.Here,we profiled the diurnal and circadian landscapes of the shoots and roots of Medicago truncatula and identified the conserved regulatory sequences contributing to transcriptome oscillations in each organ.We found that the light-dark cycles strongly affect the global transcriptome oscillation in roots,and many clock genes oscillate only in shoots.Moreover,many key genes involved in nitrogen fixation are regulated by circadian rhythms.Surprisingly,the root clock runs faster than the shoot clock,which is contrary to the hierarchical circadian structure showing a slow-paced root clock in both detached and intact Arabidopsis thaliana(L.)Heynh.roots.Our result provides important clues about the species-specific circadian regulatory mechanism,which is often overlooked,and possibly coordinates the timing between shoots and roots independent of the current prevailing model.

关键词： circadian clock hierarchical coupling structure Medicago truncatula nitrogen fixation oscillator networks

孵化湿度对胚胎期中华鳖裙边外部形态和胶原沉积的影响

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水产科学 2024年第3期43卷 445-452页

作者：傅凯琳陈培恒杨明康吴俊涛李彩燕浙江万里学院生物与环境学院浙江宁波315000

为探究孵化湿度对中华鳖营养品质的影响,采集5龄中华鳖所产的同批次受精卵[(3.05±0.05)g],在相对湿度65%和85%下于实验室全裸孵化箱中进行无接触式孵化,分别取18、20、22、24、26期胚胎各20枚,迅速解剖出裙边组织,用于荧光定量PCR分析... 详细信息

为探究孵化湿度对中华鳖营养品质的影响,采集5龄中华鳖所产的同批次受精卵[(3.05±0.05)g],在相对湿度65%和85%下于实验室全裸孵化箱中进行无接触式孵化,分别取18、20、22、24、26期胚胎各20枚,迅速解剖出裙边组织,用于荧光定量PCR分析测定胶原蛋白含量,并用天狼星红染色法进行组织学分析,研究2种孵化湿度对胚胎期中华鳖外部形态和胶原物质积累的影响。试验结果显示:随着胚胎发育,背甲指数(背甲质量/体质量)、裙边指数(裙边质量/体质量)、背甲比值(背甲长/体长)和裙边比值(体长/裙边宽)等性状总体上呈增加趋势。相对湿度85%下孵育的中华鳖胚胎在大多数发育阶段的外部形态特征性指标显著优于相对湿度65%(P<0.05)。相对湿度85%下孵育的裙边组织呈现强橙黄色或亮红色偏振光,随着胚胎发育,裙边组织Ⅰ型胶原蛋白面积的占比不断增加,极显著高于相对湿度65%下的此数值(P<0.01)。发育至第22期,相对湿度85%下孵育的裙边组织Ⅰ型胶原蛋白基因mRNA表达水平迅速增加,推测潮湿环境可能有利于胚胎期中华鳖发育过程中胶原蛋白的沉积和相关优良性状的形成。试验结果可为中华鳖生产实践中改善孵化湿度调控裙边性状发育提供基础数据。

关键词：中华鳖裙边孵化湿度胶原蛋白

A self-centering and stiffness-controlled MEMS accelerometer

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Microsystems & Nanoengineering 2024年第1期10卷 27-40页

作者： Yiming Jin Zhipeng Ma Ziyi Ye mingkang Li Xudong Zheng Zhonghe Jin School of Aeronautics and Astronautics Zhejiang UniversityHangzhou 310013China Key Laboratory of Micro/Nano-Satellite Research of Zhejiang Province Hangzhou 310007PR China

This paper presents a high-performance MEMS accelerometer with a DC/AC electrostatic stiffness tuning capability based on double-sided parallel plates(DSPPs).DC and AC electrostatic tuning enable the adjustment of the effective stiffness and the calibration of the geometric offset of the proof mass,respectively.A dynamical model of the proposed accelerometer was developed considering both DC/AC electrostatic tuning and the temperature effect.Based on the dynamical model,a self-centering closed loop is proposed for pulling the reference position of the forceto-rebalance(FTR)to the geometric center of DSPP.The self-centering accelerometer operates at the optimal reference position by eliminating the temperature drift of the readout circuit and nulling the net electrostatic tuning forces.The stiffness closed-loop is also incorporated to prevent the pull-in instability of the tuned low-stiffness accelerometer under a dramatic temperature variation.Real-time adjustments of the reference position and the DC tuning voltage are utilized to compensate for the residue temperature drift of the proposed accelerometer.As a result,a novel controlling approach composed of a self-centering closed loop,stiffness-closed loop,and temperature drift compensation is achieved for the accelerometer,realizing a temperature drift coefficient(TDC)of approximately 7μg/℃ and an Allan bias instability of less than 1μg.

关键词： stiffness electrostatic centering

云南多民族寺院大鼓和象脚鼓乐队、乐种的发展与现况研究

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星海音乐学院学报 2022年第1期 42-55页

作者：杨民康中央音乐学院

在中国云南西南部傣族、孟高棉语诸民族及相邻国家、民族地区的佛教与民俗节庆活动中,采用最多、流行最广的乐器和乐队组合是鼓(寺院大鼓或象脚鼓)、铓锣、钹三者组成的打击乐队,其表演的器乐和舞乐构成了最富于当地民族风格特色的传统... 详细信息

在中国云南西南部傣族、孟高棉语诸民族及相邻国家、民族地区的佛教与民俗节庆活动中,采用最多、流行最广的乐器和乐队组合是鼓(寺院大鼓或象脚鼓)、铓锣、钹三者组成的打击乐队,其表演的器乐和舞乐构成了最富于当地民族风格特色的传统乐种。随着近几十年民族音乐学研究思维的发展、引入和拓展深化,有关象脚鼓乐种的个案及比较研究及对国外东南亚国家相关研究资讯的介绍始逐渐增多,并且以国内外存活状况的分别研究和跨界族群音乐比较研究等不同方式展现出来。本文将结合对相关研究历史过程及概况的综述,对该类乐队、乐种的发展现状进行描写和讨论。

关键词：云南多民族寺院大鼓象脚鼓乐队乐种研究

Stay in touch with the endoplasmic reticulum

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Science China(Life Sciences) 2024年第2期67卷 230-257页

作者： Sha Sun Gan Zhao mingkang Jia Qing Jiang Shulin Li Haibin Wang Wenjing Li Yunyun Wang Xin Bian Yan G.Zhao Xun Huang Ge yang Huaqing Cai Jose C.Pastor-Pareja Liang Ge Chuanmao Zhang Junjie Hu National Laboratory of Biomacromolecules Institute of BiophysicsUniversity of Chinese Academy of SciencesChinese Academy of SciencesBeijing 100101China The Ministry of Education Key Laboratory of Cell Proliferation and Differentiation College of Life SciencesPeking UniversityBeijing 100871China State Key Laboratory of Membrane Biology Tsinghua-Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China Laboratory of Computational Biology&Machine Intelligence School of Artificial IntelligenceUniversity of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Medicinal Chemical Biology College of Life SciencesNankai UniversityTianjin 300071China Brain Research Center Department of BiologySchool of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China Institute of Neurosciences Consejo Superior de Investigaciones Científicas-Universidad Miguel HernándezSan Juan de Alicante 03550Spain

The endoplasmic reticulum(ER),which is composed of a continuous network of tubules and sheets,forms the most widely distributed membrane system in eukaryotic cells.As a result,it engages a variety of organelles by establishing membrane contact sites(MCSs).These contacts regulate organelle positioning and remodeling,including fusion and fission,facilitate precise lipid exchange,and couple vital signaling events.Here,we systematically review recent advances and converging themes on ER-involved organellar contact.The molecular basis,cellular influence,and potential physiological functions for ER/nuclear envelope contacts with mitochondria,Golgi,endosomes,lysosomes,lipid droplets,autophagosomes,and plasma membrane are summarized.

关键词： endoplasmic reticulum nuclear envelope mitochondria Golgi apparatus endosome lysosome lipid droplets autophagosome plasma membrane membrane contact site

蒺藜苜蓿生物钟基因MtTOC1a的克隆及功能验证

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华南农业大学学报 2023年第5期44卷 803-809页

作者：段婷婷杨明康黄可黄巍华南农业大学生命科学学院广东广州510642 广东省农业生物蛋白质功能与调控重点实验室/岭南现代农业科学与技术广东省实验室广东广州510642

【目的】分析蒺藜苜蓿Medicago truncatula生物钟基因MtTOC1a的蛋白结构,探究MtTOC1a在生物钟系统中的生物学功能,比较其与拟南芥Arabidopsis thaliana的AtTOC1功能相似性和差异性。【方法】通过生物信息学分析,在全基因组范围内鉴定了T... 详细信息

【目的】分析蒺藜苜蓿Medicago truncatula生物钟基因MtTOC1a的蛋白结构,探究MtTOC1a在生物钟系统中的生物学功能,比较其与拟南芥Arabidopsis thaliana的AtTOC1功能相似性和差异性。【方法】通过生物信息学分析,在全基因组范围内鉴定了TOC1在蒺藜苜蓿中的同源基因。构建MtTOC1a基因的表达载体,利用农杆菌介导法引入到拟南芥野生型Col、及相应的功能丧失突变体toc1-2中,进行遗传互补分析。【结果】MtTOC1a和MtTOC1b均具有保守的功能结构域和三维结构。遗传分析表明,在早期光形态建成中,外源转化的MtTOC1a完全恢复了toc1-2的下胚轴伸长表型,但对toc1-2的提前开花表型没有显著影响。在引入CAB::LUC报告基因的株系中,外源转化MtTOC1a在连续光照下使短周期突变体toc1-2的近日节律周期延长,但仍不能完全恢复至野生型水平。【结论】MtTOC1a和拟南芥AtTOC1的功能存在相似性,但在不同的下游调控途径中所扮演的角色存在差异。本研究结果为进一步探索MtTOC1a基因的功能,利用MtTOC1a基因改造苜蓿的重要性状提供了理论依据。

关键词：蒺藜苜蓿拟南芥 MtTOC1a 生物钟生物节律农艺性状

Storage, form, and influencing factors of karst inorganic carbon in a carbonate area in China

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Science China Earth Sciences 2024年第3期67卷 725-739页

作者： Chaochao DU Xiaoyong BAI yangbing LI Qiu TAN Cuiwei ZHAO Guangjie LUO Luhua WU Fei CHEN Chaojun LI Chen RAN Sirui ZHANG Lian XIONG Fengjiao SONG Biqin XIAO Zilin LI Yingying XUE mingkang LONG Qing LUO Xiaoyun ZHANG Minghui LI Xiaoqian SHEN Shu yang State Key Laboratory of Environmental Geochemistry Institute of GeochemistryChinese Academy of SciencesGuiyang 550081China School of Geography and Environmental Sciences Guizhou Normal UniversityGuiyang 550001China CAS Center for Excellence in Quaternary Science and Global Change Xi'an 710061China Guizhou Provincial Key Laboratory of Geographic State Monitoring of Watershed Guizhou Normal CollegeGuiyang 550018China College of Resources and Environmental Engineering Guizhou UniversityGuiyang 550025China

Carbonate rock chemical weathering carbon sinks reduce the rate of increase of the atmospheric CO_(2) concentration and global warming. However, uncertainty still exists in the estimation results of carbonate rock chemical weathering carbon sink fluxes(CCSF), and the contributions of climate change and ecological restoration to the CCSF are not clear. To this end, we compiled published site data on ion concentrations in different watersheds in China and used a classical thermodynamic dissolution model to reassess the potential and spatial and temporal patterns of the CCSF in China from 1991 to 2020. We quantified the contributions of temperature(MAT), precipitation(MAP), evapotranspiration(ET), soil water(SM), and the normalized difference vegetation index(NDVI) to the CCSF. The results revealed that(1) China's CCSF was 22.76 t CO_(2)km^(-2)yr^(-1), which was higher than the global average(15.77 t CO_(2)km^(-2)yr^(-1)). The total carbonate rock chemical weathering carbon sink(CCS) was 4772.67×10^(4)t CO_(2), contributing 14.91% of the global CCS through a carbonate rock area of 252.98×10^(4)km^(-2).(2) China's CCSF decreased gradually from southeast to northwest, with values of 33.14, 12.93, and7.27 t CO_(2)km^(-2)yr^(-1)in the southern karst, Qinghai-Tibetan karst, and northern karst regions, respectively.(3) The overall CCSF in China exhibited an increasing trend from 1991 to 2020, with a rate of increase of 0.16 t CO_(2)km^(-2)yr^(-1).(4) The contributions of the MAP, MAT, ET, SM, and NDVI to the CCSF were 63.3%, 3.02%, 27.5%, 3.1%, and 3.05%, respectively. Among them, the increase in precipitation was the main contributor to the increase in the CCSF in China over the last 30 years, while the enhancement of ET offset part of the positive contribution of the increase in precipitation to the CCSF. In conclusion, the results of this study provide a systematic quantification of the magnitude, the patterns, and the influencing factors of CCS over a long time series in China. The results are of great significance an

关键词： Carbonate rocks Carbon sinks Patterns Influencing factors Thermodynamic dissolution modeling

异源过表达蒺藜苜蓿MtATG7基因促进拟南芥抵抗碳氮饥饿胁迫

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华南农业大学学报 2023年第5期44卷 810-817页

作者：朱晓洁王贤杰杨明康黄巍陈亮华南农业大学生命科学学院广东广州510642 岭南现代农业科学与技术广东省实验室广东广州510642

【目的】真核生物通过保守的自噬途径降解错误折叠的蛋白质或受损细胞器,实现对营养物质的循环再利用。本文旨在解析苜蓿自噬基因在植物应对营养胁迫中发挥的功能,为指导苜蓿的选育和改良提供参考。【方法】以自噬途径的关键限速基因ATG... 详细信息

【目的】真核生物通过保守的自噬途径降解错误折叠的蛋白质或受损细胞器,实现对营养物质的循环再利用。本文旨在解析苜蓿自噬基因在植物应对营养胁迫中发挥的功能,为指导苜蓿的选育和改良提供参考。【方法】以自噬途径的关键限速基因ATG7(Autophagy-related gene 7)为切入点,分析ATG7氨基酸序列在不同植物中的相似性。利用蒺藜苜蓿Medicago truncatula的MtATG7基因过表达载体转化拟南芥植株,获得异源过表达植株35S::MtATG7和互补拟南芥突变体的atg7/35S::MtATG7植株,并对其抗胁迫表型和自噬活性进行分析。【结果】在碳饥饿条件下,atg7/35S::MtATG7能够挽救atg7突变体叶片早衰的表型;恢复到正常生长条件以后,35S::MtATG7和atg7/35S::MtATG7植株存活率和野生型相比都显著提高。GFP-ATG8e的剪切试验表明,atg7/35S::MtATG7能够恢复atg7突变体的自噬降解活性。在氮饥饿条件下过表达MtATG7同样能够减缓拟南芥叶片的衰老速度。【结论】异源过表达MtATG7能增强拟南芥碳氮饥饿抗性的生物学功能,本研究为进一步利用MtATG7基因改良苜蓿农艺性状提供了理论依据。

关键词：细胞自噬 MtATG 缺碳胁迫缺氮胁迫蒺藜苜蓿拟南芥异源过表达