检索结果-南通市图书馆

The biogenesis and secretion of exosomes and multivesicular bodies(MVBs):Intercellular shuttles and implications in human diseases

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2023年第5期10卷 1894-1907页

作者： Minxue Xu Jie Ji Dandan Jin Yue Wu Tong Wu Renjie Lin Shengze Zhu Feng Jiang Yifei Ji Baijun Bao Mei Li Weisong Xu mingbing xiao Department of Gastroenterology and Research Center of Clinical Medicine Affiliated Hospital and Medical School of Nantong UniversityNantongJiangsu 226001China Third People's Hospital Affiliated to Nantong University NantongJiangsu 226001China Medical School of Nantong University Oral Medcine192 NantongJiangsu 226001China Department of Medical Oncology Affiliated Hospital of Nantong UniversityNantongJiangsu 226001China Department of Gastroenterology Affiliated Nantong Rehabilitation Hospital of Nantong UniversityNantongJiangsu 226001China

Exosomes carry and transmit signaling molecules used for intercellular *** generation and secretion of exosomes is a multistep interlocking process that allows simultaneous control of multiple regulatory *** molecules,mainly RAB GTPases,cytoskeletal proteins and soluble N-ethylmaleimide-sensitive fusion attachment protein receptor(SNARE),are specifically regulated in response to pathological conditions such as altered cellular microenvironment,stimulation by pathogenic factors,or gene *** interferes with the smooth functioning of endocytosis,translocation,degradation,docking and fusion processes,leading to changes in the secretion of *** numbers of secreted exosomes are disseminated by the flow of body fluids and absorbed by the recipient *** transmitting characteristic functional proteins and genetic information produced under disease conditions,exosomes can change the physiological state of the recipient cells and their *** microenvironment,in turn,affects the occurrence and development of ***,this review will discuss the mechanism by which exosome secretion is regulated in cells following the formation of mature secretory multivesicular bodies(MVBs).The overall aim is to find ways to eliminate disease-derived exosomes at their source,thereby providing an important new basis for the clinical treatment of disease.

关键词： Cellular microenvironment Cytoskeleton Exosome secretion RAB GTPase SNARE

Clinical Value of Hepatoma-Specific Alpha-Fetoprotein in the Diagnosis of Hepatocellular Carcinoma

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Clinical Oncology 2006年第3期3卷 153-157页

作者： Runzhou Ni mingbing xiao Fei Jin Cuihua Lu Jiefei Huang Xianyong Meng Department of Gastroenterology Affiliated Hospital of Nantong University Nantong 226001 Jiangsu Province China.

OBJECTIVE To study the clinical value of hepatoma-specific alpha-feto- protein (HS-AFP) in the diagnosis and differential diagnosis of hepato- cellular carcinoma (HCC). METHODS A method of vertical slab polyacrylamide gel electrophoresis with discontinuous buffer system was developed to separate AFP sub- types. After separation, the AFP subtypes were transferred to nitrocellu- lose and reacted first with rabbit anti-human AFP and then with goat an- ti-rabbit IgG -HRP. Finally, AFP subtypes were visualized by reacting with 3,3’-diaminobenzidine tetrahydrochloride. A HS-AFP band was de- termined in 82 cases with HCC and 95 cases with benign liver diseases. The correlations between the positive rates of HS-AFP and serum AFP concentration, tumor size as well as portal vein metastasis were analyzed. RESULTS Serum AFP in the cases with various liver diseases was sepa- rated into one to several bands. The fastest band on electrophoresis (F- AFP) was found in all patients, while the band at the cathodal site (HS- AFP) was detected predominantly in HCC but rarely in benign liver dis- eases. The positive rate of HS-AFP in HCC was 74.4%, which was sig- nificantly higher than that in benign liver diseases (9.1%, 7.3% and 10.0% in liver cirrhosis, chronic hepatitis and acute hepatitis respectively). HS- AFP was detected in 3 out of 9 HCC cases with AFP<50 μg/L , but in none of 22 cases of benign liver diseases with the same AFP concentra- tion. HS-AFP correlated with serum AFP concentration and tumor size to some extent, but not with portal vein metastasis. CONCLUSION HS-AFP increases the sensitivity of diagnosing HCC in patients with negative AFP, and is useful in distinguishing high AFP due to HCC from that caused by benign liver diseases.

关键词： hepatoma alpha-fetoprotein heterogeneity diagnosis.

The Use of Postoperative Serum HS-AFP and GGTⅡfor Judgment of the Prognosis for Hepatocellular Carcinoma patients

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Clinical Oncology 2006年第4期3卷 258-261页

作者： Runzhou Ni Lei Yang mingbing xiao Feng Li Cuihua Lu Department of Gastroenterology Affiliated Hospital of Nantong University Nantong 226001 Jiangsu Province China.

OBJECTIVE To investigate the clinical value of hepatoma-specific alpha-fetoprotein （HS-AFP） and gamma-glutamyltransferase Ⅱ （GGT Ⅱ） for judgment of postoperative prognosis of patients with hepatocellular carcinoma （HCC）. METHODS HS-AFP was separated and determined using native polyacrylamide electrophoresis with a discontinuous buffer system and Western blots. GGT Ⅱ was separated with native polyacrylamide electrophoresis with a discontinuous buffer system and detected by enzyme staining. Forty cases with HCC underwent serial determination of HS-AFP and GGT Ⅱ before and after radical excision. The correlations were analyzed between the two indices and survival time. RESULTS In the 40 cases with HCC, before radical excision the positive rates of HS-AFP and GGT II were 57.5% and 67.5% respectively, with the positive rate of combined HS-AFP and GGT Ⅱ reaching 80.0%. After operation, the recurrence and metastasis rate in the groups with positive HS-AFP and GGT Ⅱ were 90.9% and 58.8% respectively, while in the groups with negative HS-AFP and GGT Ⅱ the rates were 20.7% and 26.1% respectively. Recurrence and metastasis occurred in all cases with both postoperative positive HS-AFP and GGT Ⅱ but only in 9.5% of the cases in whom both postoperative HS-AFP and GGT Ⅱ were negative. Univariate analysis revealed that postoperative HS-AFP and GGT Ⅱ were related to the prognosis in HCC. CONCLUSION Postoperative serum HS-AFP and GGT Ⅱ are very useful in predicting the prognosis of HCC patients.

关键词： alpha-fetoprotein heterogeneity gamma-glutamytkansferase hepatocellular carcinoma prognosis.

Correlation between Thymidylate Synthase Genotype and Susceptibility to Gastric Carcinoma

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Clinical Oncology 2008年第6期5卷 448-452页

作者： Lei Yang mingbing xiao Runzhou Ni Qinghe Tan Jinzhi Wei Jianhong Wang Bojian Ge Department of Medicine Tumor Hospitalof Nantong University Nantong 226361Jiangsu Province China.

OBJECTIVE To investigate the correlation between polymorphism of the 5′-untranslated region （5′-UTR） of thymidylate synthase genes, as well as the lifestyle, and the susceptibility of gastric carcinoma. METHODS A case-control study, with 60 cases of gastric carcinoma and 170 cases of general risk population-based controls from Nantong, Jiangsu province, China, was conducted. The epidemiological data, such as living habits of the cancer patients, were collected. DNA of peripheral blood leukocytes was obtained from all of the subjects. The TS 5′-UTR tandem repeat genotype was detected using polymerase chain reaction （PCR）. RESULTS There were three TS 5′-UTR genotypes in the group of gastric cancer cases （2R/2R, 2R/3R and 3R/3R） and six TS 5′-UTR genotypes in the group of the controls （2R/2R, 2R/3R, 3W3R, 2R/4R, 2R/5R and 3R/4R）. The genotypic frequencies were respectively 5.0%, 43.3% and 51.7% in the gastric cancer group. Compared with the parameters in the control group, i.e., 4.7%, 31.7%, 60.6%, 1.2%, 1.2% and 0.6%. There were no significant differences between the two groups. Compared with the 3R/3R- genotype individuals who where non-smokers, drank alcohol twice or less each week, drank tea and did not intake pickled food （PF）, the risk of gastric cancer significantly went up in the 2R/2R or 2R/3R-genotype people who had habits of smoking, drinking alcohol more than twice each week, no tea drinking but with frequent intake of PF. The adjusted ORs were as follows, 3.79 （95% CI： 2.45-8.64）, 3.41 （95% CI and 3.61 （95% CI： 1.81-8.78）. CONCLUSION There is 1.21-8.47）, 5.99 （95% Ch 3.01-14.7）, an obvious correlation between the polymorphisms of TS 5′-UTR genotypes and the lifestyle of individuals in the development of gastric carcinoma.

关键词： gastric cancer thymidylate synthase genotype smoking alcohol drinking tea drinking pickled food.

Expression of TRAIL and its receptors in primary hepatic carcinoma and apoptosis-inducing effect of HrsTRAIL on hepatoma cell line HepG2

在线全文

学校读者我要写书评

暂无评论

Journal of Nanjing Medical University 2006年第6期20卷 372-376页

作者： mingbing xiao Jiefei Huang Runzhou Ni Jing Zhu Hong Zhang Qun Wei Feng Jiang Baijun Bao Department of Gastroenterology Affiliated Hospital of Nantong University Nantong 226001 China

Objective： To investigate the expression of tumor necrosis factor-related apoptosis-inducing ligand（TRAIL） and its receptors in primary hepatic carcinoma （PHC） and the apoptosis-inducing effect on hepatoma cell line HepG2. Methods： TRAIL and its receptors were detected by semiquantitive RT-PCR in 30 PHC and para-carcinoma tissues and two hepatoma cell lines of HepG2 and SMMC-7721. HepG2 cells were treated with human recombinant soluble TRAIL protein （HrsTRAIL） and then the viability of HepG2 cells was measured by microculture tetrazolium dye（MTT） assay and apoptosis index was demonstrated by fluorescence-activated cell sorting （FACS）. Results：TRAIL and its receptors were detectable in all PHC and para-carcinoma tissues and hepatoma cell line HepG2. TRAIL, death receptor 4 （DR4）, DR5, and decoy receptor 2 （DcR2） but not DcR1 were detectable in hepatoma cell line SMMC-7721. The expression patterns of TRAIL receptors in HepG2 were quite similar to PHC specimens. The semiquantitive results showed that the expression level of TRAIL and DcR were lower but DR was higher in hepatoma tissues than in para-carcinoma tissues. In PHC tissues, the expressions of DR were higher than DcR, while there was no difference in para-carcinoma tissues. HrsTRAIL had potent antitumor activity in a time- and dose-dependent manner. After co-incubations of the HepG2 cells in the presence of HrsTRAIL at concentration 1 000 ng/ml for 24 hours, the viability of HepG2 cells decreased to 45% and the apoptosis index reached 51%. Conclusion ：TRAIL and its receptors were expressed in both PHC tissues and para-carcinoma tissues but the expression levels were different. The lower expression of TRAIL in PHC tissues suggested that insufficient apoptosis occured in the development of PHC. High expression of DR in PHC tissues may be a self-defense mech- anism and may afford a theory of HrsTRAIL therapy for PHC. HrsTRAIL may be a potential cytotoxic drug for PHC, and it can kill majority of HepG2 cells, but m

关键词： carcinoma TRAIL receptor RT-PCR human recombination protein apoptosis

HS-AFP在肝癌早期诊断及鉴别诊断中的价值

在线全文

学校读者我要写书评

暂无评论

中国肿瘤临床 2008年第15期35卷 845-847页

作者：贲其稳倪润洲肖明兵陆翠华李立人毛振彪南通大学附属医院消化内科江苏省南通市226001

目的：探讨肝癌特异性甲胎蛋白（HS—AFP）对肝细胞癌的诊断及鉴别诊断价值。方法：对105例肝癌患者及151例良性肝病血清HS—AFP及AFP进行检测，并对10例HS—AFP阳性的良性肝病患者进行15个月随访观察。HS—AFP采用不连续缓冲系统PAGE结... 详细信息

目的：探讨肝癌特异性甲胎蛋白（HS—AFP）对肝细胞癌的诊断及鉴别诊断价值。方法：对105例肝癌患者及151例良性肝病血清HS—AFP及AFP进行检测，并对10例HS—AFP阳性的良性肝病患者进行15个月随访观察。HS—AFP采用不连续缓冲系统PAGE结合Westernblot分离检测，AFP采用化学发光法测定。结果：105例肝癌患者HS—AFP和AFP〉200μg／L阳性率分别为60．0％和50．5％（P〉0．05），良性肝病患者HS—AFP阳性率显著低于AFP〉200μg／L的阳性率（P〈0．05）。AFP处于50～400μg／L范围的肝癌组HS—AFP阳性率为77．1％，良性肝病组阳性率为13．6％（P〈0．01），151例良性肝病中有10例HS-AFP出现阳性，其中3例于随访期间检出肝癌。11例小体积肝癌HS—AFP阳性率（45．5％）高于AFP〉200μg／L的阳性率（18．2％）。结论：HS—AFP对肝癌的诊断价值优于AFP浓度。对肝癌高危人群监测HS—AFP有助于肝癌的早期诊断。对血清AFP中低浓度升高的良恶性肝病有较高的鉴别诊断价值、

关键词：甲胎蛋白异质体肝细胞癌诊断

医院教管结合型优质科研管理信息平台构建与应用效果

在线全文

学校读者我要写书评

暂无评论

中华医学科研管理杂志 2022年第1期35卷 71-75页

作者：潘玲玲陈飞徐水珠施维肖明兵南通大学附属医院科技处 226001

目的探索更高效、规范的科研管理模式,以达到规范科研人员行为、提升科研人员主动性及满意度,同时提高科研管理工作效率的目的。方法根据科研管理过程中存在的问题与不足,结合最新科研管理政策导向,形成医院科研管理平台研发理念,并利... 详细信息

目的探索更高效、规范的科研管理模式,以达到规范科研人员行为、提升科研人员主动性及满意度,同时提高科研管理工作效率的目的。方法根据科研管理过程中存在的问题与不足,结合最新科研管理政策导向,形成医院科研管理平台研发理念,并利用信息化手段研发符合要求的科研管理信息平台。结果应用教管结合型科研管理信息平台后,科研人员报销、查阅信息等所需要的时间均明显缩短,差异具有显著统计学意义。结论教管结合型科研信息平台的构建有助于实现高效、规范和精细化的科研管理工作目标。

关键词：医院规范管理优质科研信息平台教管结合提高认知

联合检测ANXA2和RACK1在肝细胞肝癌预后判断中的价值

在线全文

学校读者我要写书评

暂无评论

中国肿瘤临床 2022年第6期49卷 286-292页

作者：邱婷婷周梦肖明兵瞿利帅倪润洲刘金霞南通大学附属医院消化内科江苏省南通市226001

目的:联合检测膜联蛋白A2(ANXA2)和活化的蛋白激酶C的受体1(RACK1)在肝癌及癌旁组织中的表达及其预后价值。方法:收集2010年1月至2011年12月南通大学附属医院行肝癌根治性切除术100例患者的石蜡标本。通过免疫组织化学染色检测ANXA2和RA... 详细信息

目的:联合检测膜联蛋白A2(ANXA2)和活化的蛋白激酶C的受体1(RACK1)在肝癌及癌旁组织中的表达及其预后价值。方法:收集2010年1月至2011年12月南通大学附属医院行肝癌根治性切除术100例患者的石蜡标本。通过免疫组织化学染色检测ANXA2和RACK1在肝细胞癌中的表达,分析其与生存和复发时间的相关性,探讨两者联合表达在肝细胞癌中的预后价值。结果:免疫组织化学结果提示ANXA2与RACK1的联合表达水平与肿瘤分化、TNM分期和脉管癌栓有关(均P0.05)。RACK1表达与肿瘤分化(P0.05);双变量Kendall检验结果显示,ANXA2和RACK1的表达水平存在显著正相关(Z=0.419,P<0.01)。ANXA2或RACK1的高表达提示有早期复发的倾向,在12例早期复发患者(复发时间<12个月)中,11例患者高表达ANXA2(11/12,91.7%)和RACK1(11/12,91.7%)。Kaplan-Meier分析结果提示,ANXA2-/RACK1-患者的总生存率显著高于ANXA2+/RACK1-、ANXA2-/RACK1+和ANXA2+/RACK1+患者;ANXA2+/RACK1+患者较ANXA2+/RACK1-、ANXA2-/RACK1+和ANAX2-/RACK1-患者更易早期复发。结论:ANXA2和RACK1是预测肝癌患者生存和复发的独立因素,两者联合检测更加有助于预后的判断。

关键词：膜联蛋白A2 活化的蛋白激酶C的受体1 肝细胞肝癌预后

2001年—2020年江苏省部分高校及其附属医院专利分析及管理对策研究

在线全文

学校读者我要写书评

暂无评论

中国医疗管理科学 2022年第6期12卷 17-23页

作者：陈飞肖明兵徐水珠南通大学附属医院科技处南通226001

目的分析近20年来江苏省省会城市、苏南、苏中及苏北地区部分高校及其第一附属医院的专利申请及授权情况,以期为医院的专利管理提供决策依据。方法通过国家知识产权官网专利信息数据库,获取2001年1月1日至2020年12月31日4所高校:南京医... 详细信息

目的分析近20年来江苏省省会城市、苏南、苏中及苏北地区部分高校及其第一附属医院的专利申请及授权情况,以期为医院的专利管理提供决策依据。方法通过国家知识产权官网专利信息数据库,获取2001年1月1日至2020年12月31日4所高校:南京医科大学、苏州大学、南通大学、徐州医科大学,以及这4所高校的第一附属医院,即:江苏省人民医院、苏州大学第一附属医院、南通大学附属医院、徐州医科大学附属医院的专利申请与授权情况、专利类型、授权专利第一发明人所在学科、IPC分类、有效状态等数据。采用SPSS25.0软件进行统计分析。结果2001年—2020年期间,检索到4所高校及其附属医院专利申请量为20792项,授权量为12405项。各高校及其附属医院申请(除2020年外)和授权(所有年份)专利数量基本是随着年份的增长呈现上升的趋势(P均<0.05)。2001年—2005年,4所附属医院申请和授权专利数量较少。所有授权专利中,有权(审定授权)7490项,占60.38%。其中南通大学附属医院有效专利277项,有效率35.56%,较其他医院低,差异有统计学意义(χ^(2)=333.96,P<0.001)。授权专利中,发明专利6235项,占授权总数的50.30%;实用新型专利5765项,占授权总数的46.50%,占比最高;外观设计专利405项,占比3.20%。比较高校及其附属医院的授权专利类型分布情况,发现高校专利以发明专利为主,附属医院以实用新型为主。结论高校和附属医院专利的特点不一致,但在专利类型、专利有效率、综合管理等方面均有待提升。管理层应针对不同的特点,改善并完善专利管理体制运行机制,营造良好的创新创优环境,鼓励更多发明创造的产出及转化。

关键词：高校高校附属医院专利分析管理对策江苏省