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氧化铁磁性纳米颗粒介导缺氧诱导因子1α shRNA质粒转染逆转A549/CDDP细胞对顺铂耐药的实验研究

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生物化学与生物物理进展 2010年第10期37卷 1090-1100页

作者：涂馨闵凌峰陈琼谢明萱何玲玲中南大学湘雅医院老年医学科呼吸内科长沙410008

评价了氧化铁磁性纳米颗粒作为缺氧诱导因子1α shRNA(HIF-1α shRNA)重组质粒载体进行体内体外转染的可行性及其逆转人肺腺癌耐药细胞株(A549/CDDP)顺铂耐性的效果,并初步探讨相关机制.在成功构建HIF-1α shRNA重组质粒后,分别利用氧... 详细信息

评价了氧化铁磁性纳米颗粒作为缺氧诱导因子1α shRNA(HIF-1α shRNA)重组质粒载体进行体内体外转染的可行性及其逆转人肺腺癌耐药细胞株(A549/CDDP)顺铂耐性的效果,并初步探讨相关机制.在成功构建HIF-1α shRNA重组质粒后,分别利用氧化铁磁性纳米颗粒及脂质体介导质粒转染A549/CDDP细胞及其移植瘤裸鼠动物模型,荧光显微镜检测转染效率,RT-PCR及细胞免疫化学检测转染后A549/CDDP细胞HIF-1α、多药耐药相关蛋白1(multidrug resistance1,MRP1)、肺耐药相关蛋白(lung resistance-related protein,LRP)mRNA及蛋白质表达,免疫组化检测转染后A549/CDDP移植瘤HIF-1α、MRP1及LRP蛋白表达,MTT法检测转染HIF-1α shRNA前后A549/CDDP细胞对顺铂的耐药性,并检测转染HIF-1α shRNA后A549/CDDP细胞移植瘤的肿瘤生长指数,he染色检测纳米颗粒转染对裸鼠肝、肾及脑的组织学毒理作用.结果显示,氧化铁磁性纳米颗粒介导转染相对效率高于脂质体介导(P<0.01),HIF-1α shRNA转染A549/CDDP细胞后HIF-1α、MRP1、LRP mRNA及蛋白质表达下降,逆转A549/CDDP细胞对顺铂耐性82%,纳米颗粒介导HIF-1α shRNA转染A549/CDDP细胞移植瘤裸鼠动物模型后,移植瘤组织中HIF-1α、MRP1及LRP蛋白表达下降,转染HIF-1α shRNA后抑制移植瘤的生长,且与顺铂有协同效应,纳米颗粒转染后裸鼠肝、肾及脑组织无坏死.这些结果说明,纳米颗粒介导HIF-1α shRNA的体内体外转染效率高于脂质体,RNA干扰技术封闭HIF-1α基因可较大程度逆转耐顺铂人肺腺癌细胞的耐药性及其抑制瘤的生长,其作用可能与HIF-1α shRNA降低HIF-1α、MRP1及LRP表达有关,HIF-1α基因可作为逆转肺癌耐药治疗的有效靶点,磁性纳米颗粒介导HIF-1α shRNA质粒转染具有一定的生物安全性.

关键词：氧化铁磁性纳米颗粒肺肿瘤基因转染缺氧诱导因子1α(HIF-1d) RNA干扰

Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction

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World Journal of Gastroenterology 2005年第34期11卷 5385-5389页

作者： Wei-Ping Qian Yue-Qiu Tan Ying Chen Ying Peng Zhi Li Guang-Xiu Lu Made C. Liu Hsiang-Fu Kung ming-ling he Li-Ka Shing Luofu Hospital Shenzhen 518033 Guangdong Province China Institute of Reproduction and Stem Cell Engineering Xiangya School of Medicine Central South University Changsha 410008 Hunan Province China The Institute of Molecular Biology The University of Hong Kong Hong Kong Special AdministrativeRegion China Department of Neurology The Second AffiliatedHospital Sun Yat-Sen University Guangzhou 510120 Guangdong Province China The Center for Emerging Infectious Diseases The Chinese University ofHong Kong Hong Kong Special Administrative Region China

AIM： To examine the sensitivity and accuracy of real-time polymerase chain reaction （PCR） for the quantification of hepatitis B virus （HBV） DNA in semen. METHODS： hepatitis B viral DNA was isolated from HBV carriers＇ semen and sera using phenol extraction method and QIAamp DNA blood mini kit （Qiagen, Germany）. HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based real-time PCR （quantitative polymerase chain reaction （qPCR））. The detection threshold was 200 copies of HBV DNA for conventional PCR and 10 copies of HBV DNA for real time PCR per reaction. RESULTS： Both methods of phenol extraction and QIAamp DNA blood mini kit were suitable for isolating HBV DNA from semen. The value of the detection thresholds was 500 copies of HBV DNA per mL in the semen. The viral loads were 7.5×10^7 and 1.67×10^7 copies of HBV DNA per mL in two HBV infected patients＇ sera, while 2.14×10^5 and 3.02×10^5 copies of HBV DNA per mL in the semen. CONCLUSION： Real-time PCR is a more sensitive and accurate method to detect and quantify HBV DNA in the semen.

关键词： hepatitis B virus Semen Real-time polymerase chain reaction Viral load

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加强贵重仪器设备使用管理的思考

实验室研究与探索 2006年第1期25卷 108-110,121页

作者：林明河中山大学设备与实验室管理处广东广州510275

针对高校贵重仪器设备的使用管理现状以及存在的问题,提出了包括管理体制改革、运行机制创新、大平台建设、资源共享、监控评估、强化购前论证以及技术队伍建设等一系列具有理念性和实践性的改革思路。在这些思路中,作者已在该校做了尝... 详细信息

针对高校贵重仪器设备的使用管理现状以及存在的问题,提出了包括管理体制改革、运行机制创新、大平台建设、资源共享、监控评估、强化购前论证以及技术队伍建设等一系列具有理念性和实践性的改革思路。在这些思路中,作者已在该校做了尝试,取得了较好的效果。

关键词：贵重仪器设备使用与管理

宫颈癌治疗后^(18)F-氟脱氧葡萄糖PET/CT检查与鳞状细胞癌抗原水平的关系研究

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中国全科医学 2016年第30期19卷 3667-3671页

作者：张召奇赵新明和玲燕河北医科大学第四医院核医学科河北省石家庄市050011

背景^(18)F-氟脱氧葡萄糖(^(18)F-FDG)PET/CT检查在宫颈癌治疗后血清鳞状细胞癌(SCC)抗原水平升高患者中具有重要应用价值。对于宫颈癌治疗后临床怀疑复发及转移但SCC抗原水平处于参考范围内的患者行^(18)F-FDG PET/CT检查有无意义,以... 详细信息

背景^(18)F-氟脱氧葡萄糖(^(18)F-FDG)PET/CT检查在宫颈癌治疗后血清鳞状细胞癌(SCC)抗原水平升高患者中具有重要应用价值。对于宫颈癌治疗后临床怀疑复发及转移但SCC抗原水平处于参考范围内的患者行^(18)F-FDG PET/CT检查有无意义,以及宫颈癌治疗后,SCC抗原处于何种水平时适宜进行^(18)F-FDG PET/CT检查可以得到理想的病灶检出率,尚未见相关报道。目的探讨宫颈癌治疗后患者SCC抗原居于何种水平时适宜进行^(18)F-FDG PET/CT检查。方法回顾性分析2008年1月—2011年12月河北医科大学第四医院收治的78例宫颈癌患者的临床资料,均为SCC,并行根治性治疗(手术、手术+术后放疗、手术+术后化疗、手术+术后放化疗等),治疗后均进行了^(18)F-FDG PET/CT检查和SCC抗原水平测定。依据^(18)F-FDG PET/CT检查结果将患者分为PET/CT阳性组(62例)和PET/CT阴性组(16例)。依据SCC抗原水平将患者分成3组:6.00μg/L组(23例)。本组入选患者^(18)F-FDG PET/CT检查结果均经病理或随访结果证实。绘制SCC抗原水平预测^(18)F-FDG PET/CT阳性的ROC曲线。结果 PET/CT阳性组患者年龄、SCC抗原水平小于PET/CT阴性组(P6.00μg/L组。^(18)F-FDG PET/CT阳性率分别为66.7%、77.4%、95.7%,3组^(18)F-FDG PET/CT阳性率比较,差异有统计学意义(P6.00μg/L组^(18)F-FDG PET/CT阳性率高于0.05)。通过ROC曲线分析,SCC抗原水平预测^(18)F-FDG PET/CT为阳性的最佳截断值为1.15μg/L,此时,灵敏度为83.6%,特异度为47.1%,本组患者在该截断值^(18)F-FDG PET/CT阳性率为86.7%。结论^(18)F-FDG PET/CT检查在SCC抗原水平较低时即可发现宫颈鳞癌复发及转移,患者SCC抗原水平升高时^(18)F-FDG PET/CT阳性率升高,当SCC抗原水平升至1.15μg/L以上时,适宜通过^(18)F-FDG PET/CT检查进行确诊。

关键词：宫颈肿瘤断层摄影术,螺旋计算机氟脱氧葡萄糖F18 癌,鳞状细胞

Mechanism of inflammatory response and therapeutic effects of stem cells in ischemic stroke:current evidence and future perspectives

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Neural Regeneration Research 2025年第1期20卷 67-81页

作者： Yubo Wang Tingli Yuan Tianjie Lyu ling Zhang Meng Wang Zhiying he Yongjun Wang Zixiao Li Vascular Neurology Department of NeurologyBeijing Tiantan HospitalCapital Medical UniversityBeijingChina China National Clinical Research Center for Neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Shanghai Engineering Research Center of Stem Cells Translational Medicine ShanghaiChina National Center for healthcare Quality Management in Neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Institute for Regenerative Medicine Shanghai East HospitalSchool of Life Sciences and TechnologyTongji UniversityShanghaiChina Chinese Institute for Brain Research BeijingChina Research Unit of Artificial Intelligence in Cerebrovascular Disease Chinese Academy of Medical SciencesBeijingChina Beijing Engineering Research Center of Digital healthcare for Neurological Diseases BeijingChina

Ischemic stroke is a leading cause of death and disability worldwide,with an increasing trend and tendency for onset at a younger ***,in particular,bears a high burden of stroke *** recent years,the inflammatory response after stroke has become a research hotspot:understanding the role of inflammatory response in tissue damage and repair following ischemic stroke is an important direction for its *** review summarizes several major cells involved in the inflammatory response following ischemic stroke,including microglia,neutrophils,monocytes,lymphocytes,and ***,we have also highlighted the recent progress in various treatments for ischemic stroke,particularly in the field of stem cell ***,understanding the complex interactions between inflammation and ischemic stroke can provide valuable insights for developing treatment strategies and improving patient *** cell therapy may potentially become an important component of ischemic stroke treatment.

关键词： cell therapy immune cell inflammatory ischemic stroke stem cell

Progress of research in the application of ultrasound technology for the treatment of Alzheimer’s disease

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Neural Regeneration Research 2025年第10期20卷 2823-2837页

作者： Qiuquan Cai Lianghui Meng Meina Quan ling Wang Jing Ren Chenguang Zheng Jiajia Yang Dong ming Academy of Medical Engineering and Translational Medicine Tianjin UniversityTianjinChina Innovation Center for Neurological Disorders Xuanwu HospitalCapital Medical UniversityBeijingChina Brain-Computer Interaction and Human-Machine Fusion Haihe Laboratory Tianjin UniversityTianjinChina Tianjin Key Laboratory of Brain Science and Neural Engineering Tianjin UniversityTianjinChina

Alzheimer’s disease is a common neurodegenerative disorder defined by decreased reasoning abilities,memory loss,and cognitive *** presence of the blood-brain barrier presents a major obstacle to the development of effective drug therapies for Alzheimer’s *** use of ultrasound as a novel physical modulation approach has garnered widespread attention in recent *** a safe and feasible therapeutic and drug-delivery method,ultrasound has shown promise in improving cognitive *** article provides a summary of the application of ultrasound technology for treating Alzheimer’s disease over the past 5 years,including standalone ultrasound treatment,ultrasound combined with microbubbles or drug therapy,and magnetic resonance imaging-guided focused ultrasound *** is placed on the benefits of introducing these treatment methods and their potential *** found that several ultrasound methods can open the blood-brain barrier and effectively alleviate amyloid-βplaque *** believe that ultrasound is an effective therapy for Alzheimer’s disease,and this review provides a theoretical basis for future ultrasound treatment methods.

关键词： Alzheimer’s disease blood-brain barrier drugs magnetic resonance imaging-guided focused ultrasound microbubbles scanning ultrasound ultrasound ultrasound stimulation

Regulation and function of endoplasmic reticulum autophagy in neurodegenerative diseases

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Neural Regeneration Research 2025年第1期20卷 6-20页

作者： Xiu-Yun Zhao De-En Xu ming-Lei Wu Ji-Chuan Liu Zi-ling Shi Quan-Hong Ma Department of Neurology and Clinical Research Center of Neurological Disease the Second Affiliated Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina Institute of Neuroscience&Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases Soochow UniversitySuzhouJiangsu ProvinceChina Department of Neurology Jiangnan University Medical CenterWuxiJiangsu ProvinceChina

The endoplasmic reticulum,a key cellular organelle,regulates a wide variety of cellular *** reticulum autophagy,one of the quality control systems of the endoplasmic reticulum,plays a pivotal role in maintaining endoplasmic reticulum homeostasis by controlling endoplasmic reticulum turnover,remodeling,and *** this review,we briefly describe the endoplasmic reticulum quality control system,and subsequently focus on the role of endoplasmic reticulum autophagy,emphasizing the spatial and temporal mechanisms underlying the regulation of endoplasmic reticulum autophagy according to cellular *** also summarize the evidence relating to how defective or abnormal endoplasmic reticulum autophagy contributes to the pathogenesis of neurodegenerative *** summary,this review highlights the mechanisms associated with the regulation of endoplasmic reticulum autophagy and how they influence the pathophysiology of degenerative nerve *** review would help researchers to understand the roles and regulatory mechanisms of endoplasmic reticulum-phagy in neurodegenerative disorders.

关键词： autophagy endoplasmic reticulum endoplasmic reticulum autophagy endoplasmic reticulum quality control system endoplasmic reticulum receptors endoplasmic reticulum-associated degradation neurodegeneration neurodegenerative disease selective autophagy unfolded protein response

Return to work in young and middle-aged colorectal cancer survivors:Factors influencing self-efficacy,fear,resilience,and financial toxicity

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World Journal of Gastroenterology 2025年第1期31卷 79-92页

作者： Dan Hu Yue Li Hua Zhang Lian-Lian Wang Wen-Wen Liu Xin Yang ming-Zhao Xiao Hao-ling Zhang Juan Li Department of Nursing The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Nursing National Clinical Research Center for Child Health and DisordersMinistry of Education Key Laboratory of Child Development and DisordersChildren’s Hospital of Chongqing Medical UniversityChongqing 400014China Department of Gastrointestinal Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Biomedical Science Advanced Medical and Dental InstituteUniversity Sains MalaysiaPenang 13200Malaysia Academic Affairs Office The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China

BACKGROUND Return to work(RTW)serves as an indication for young and middle-aged colorectal cancer(CRC)survivors to resume their normal social ***,these survivors encounter significant challenges during their RTW ***,scientific research is necessary to explore the barriers and facilitating factors of returning to work for young and middle-aged CRC *** To examine the current RTW status among young and middle-aged CRC survivors and to analyze the impact of RTW self-efficacy(RTW-SE),fear of progression(FoP),ehealth literacy(eHL),family resilience(FR),and financial toxicity(FT)on their RTW *** A cross-sectional investigation was adopted in this *** September 2022 to February 2023,a total of 209 participants were recruited through a convenience sampling method from the gastrointestinal surgery department of a class A tertiary hospital in *** investigation utilized a general information questionnaire alongside scales assessing RTW-SE,FoP,eHL,FR,and *** analyze the factors that influence RTW outcomes among young and middle-aged CRC survivors,Cox regression modeling and Kaplan-Meier survival analysis were *** A total of 43.54%of the participants successfully returned to work,with an average RTW time of 100 *** regression univariate analysis revealed that RTW-SE,FoP,eHL,FR,and FT were significantly different between the non-RTW and RTW groups(Phermore,Cox regression multivariate analysis identified per capita family monthly income,job type,RTW-SE,and FR as independent influencing factors for RTW(Phe RTW rate requires further *** levels of RTW-SE and FR were found to significantly increase RTW among young and middle-aged CRC *** professionals should focus on modifiable factors,such as RTW-SE and FR,to design targeted RTW support programs,thereby facilitating their timely reintegration into mainstream society.

关键词： Return to work Colorectal neoplasms Return-to-work self-efficacy Fear of progression Family resilience Financial toxicity

Correlations of the expression of Cx43,SCFFBXW7,p-cyclin E1(Ser73),p-cyclin E1(Thr77)and p-cyclin E1(Thr395)in colon cancer tissues

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 207-213页

作者： Rong-Gang Luan ming-Da Liu Zi-Feng Deng Cong-Lan Lu Mei-ling Yu ming-Yu Zhang Rong Liu Ran An You-Liang Yao Dong-Bei Guo Yong-Xing Zhang Lei Zhao Department of Gastrointestinal Surgery Air Force Hospital of Eastern TheaterAnhui Medical UniversityNanjing 230032Jiangsu ProvinceChina State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics School of Public HealthXiamen UniversityXiamen 361102Fujian ProvinceChina Trauma Surgery Air Force Hospital of Eastern TheaterAnhui Medical UniversityNanjing 230032Jiangsu ProvinceChina Department of Orthopaedics Air Force Hospital of Eastern TheaterAnhui Medical UniversityNanjing 230032Jiangsu ProvinceChina

BACKGROUND Previous cellular studies have demonstrated that elevated expression of Cx43 promotes the degradation of cyclin E1 and inhibits cell proliferation through ***,reduced expression results in a loss of this capacity to facilitate cyclin E *** ubiquitination and degradation of cyclin E1 may be associated with phosphorylation at specific sites on the protein,with Cx43 potentially enhancing this process by facilitating the phosphorylation of these critical *** To investigate the correlation between expression of Cx43,SKP1/Cullin1/F-box(SCF)FBXW7,p-cyclin E1(ser73,thr77,thr395)and clinicopathological indexes in colon *** Expression levels of Cx43,SCFFBXW7,p-cyclin E1(ser73,thr77,thr395)in 38 clinical colon cancer samples were detected by immunohistochemistry and were analyzed by statistical methods to discuss their *** Positive rate of Cx43,SCFFBXW7,p-cyclin E1(Ser73),p-cyclin E1(Thr77)and p-cyclin E1(Thr395)in detected samples were 76.32%,76.32%,65.79%,5.26%and 55.26%*** expressions of these proteins were not related to the tissue type,degree of tissue differentiation or lymph node ***43 and SCFFBXW7(r=0.749),p-cyclin E1(Ser73)(r=0.667)and p-cyclin E1(Thr395)(r=0.457),SCFFBXW7 and p-cyclin E1(Ser73)(r=0.703)and p-cyclin E1(Thr395)(0.415)were correlated in colon cancer(Phe above proteins were positively correlated in colon *** Cx43 may facilitate the phosphorylation of cyclin E1 at the Ser73 and Thr195 sites through its interaction with SCFFBXW7,thereby influencing the ubiquitination and degradation of cyclin E1.

关键词： Colon cancer Cx43 SCFFBXW7 Phosphorylation of cyclin E1 Sites of cyclin E1 Correlation analysis