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A Comparative Analysis of Embryo and Endosperm Proteome from Seeds of Jatropha curcas

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Journal of Integrative Plant Biology 2009年第9期51卷 850-857页

作者： Hui Liu Yu-Jun Liu ming-feng yang Shi-Hua Shen Institute of Botany the Chinese Academy of Sciences Beijing 100093 China Graduate University of the Chinese Academy of Sciences Beijing 100049 China

Jatropha curcas is an important economic plant for biodiesel, which is extracted mainly from the endosperm of its mature seeds. Despite the morphological and functional differences between the embryo and endosperm, proteomic characteristics of the two tissues are not yet known. Similar proteomic profiles were observed in the two-dimensional gel electrophoresis maps from the two tissues. There were 380 and 533 major protein spots in the embryo and endosperm, respectively. Fourteen identical spots, showing a notable change, were selected and identified by tandem mass spectrometry. Among these proteins, dihydrolipoamide acetyltransferase （spot 27） participates in tricarboxylic acid cycle, which is an amphibolic pathway. The two parts both included proteins related to stress （spots 8, 115, 118, 125, 130） and signal transduction （spots 7, 100, 108）. According to the volume percentage of proteins in embryo and endosperm, the proteins in endosperm （spots 54, 61, 73） were catabolism-related enzymes and reserves to provide the nutrition for seed germination; the proteins in embryo （spots 27, 62, 122） were inclined to anabolism and utilized the nutrition from the endosperm to generate a new life.

关键词： embryo endosperm Jatropha curcas liquid chromatography/tandem mass spectrometry proteome.

The QseB/QseC two-component system contributes to virulence of Actinobacillus pleuropneumoniae by downregulating apf gene cluster transcription

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Animal Diseases 2022年第1期2卷 33-44页

作者： Benzhen Duan Wei Peng Kang Yan feng Liu Jia Tang feng ming yang Huanchun Chen Fangyan Yuan Weicheng Bei State Key Laboratory of Agricultural M1crob1ology College of Veterinary MedicineHuazhong Agricultural UniversityWuhanChina The Cooperative Innovation Center for Sustainable Pig Production Huazhong Agricultural UniversityWuhanChina Key Laboratory of Prevention and Control Agents for Animal Bacteriosis(Ministry of Agriculture) Institute of Animal Husbandry and Veterinary SciencesHubei Academy of Agricultural SciencesWuhanChina Hube1 Hongshan Laboratory WuhanChina Guangxi yangxiang Co. LtdGu1gangChina

Actinobacillus pleuropneumoniae(APP)is the major pathogen of porcine contagious pleuropneumoniae(PCP).The QseB/QseC two-component system(TCS)consists of the regulator QseB and the kinase QseC,which relates to quorum sensing(QS)and virulence in some ***,we investigated the role of QseB/QseC in apf gene cluster(apfABCD)expression of *** results have showed that QseB/QseC TCS can potentially regulate the expression of apf gene ***△qseBC,△apfA,△apfB,△apfC and△apfD strains are more sensitive to acidic and osmotic stressful conditions,and exhibite lower biofilm formation ability than wild-type(WT)strain,whereas the complemented strains show similar phenotype to the wr *** additon,the mutants have defective antiphagocytosis,adhesion and invasion when they come into contact with the host *** experimental animal models of infection,mice infected with△qseBC,△apfA,△apfB,△apfC and △apfD strains showed lower mortality and bacterial loads in the lung and the blood than those infected with wr *** conclusion,our results suggest that QseB/QseC TCS contributes to stress resistance,biofilm formation,phagocytosis,adhesion,invasion and virulence by downregulating expression of apf gene cluster in ***.

关键词： A.pleuropneumoniae QseB/QseC Transcriptional regulation apf gene cluster Virulence

Charge regulation by ferromagnetic metal/LiF spin-polarized interface for high-performance Li metal anodes

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Rare Metals 2024年第3期43卷 995-1005页

作者： ming-yang feng Shuang Zhou Yi-Fang Zhang Ya-Ping Wang Chun-Yan Fu Jian-Wen Li Yuan-Lang Wan Zhi-Jia Zhang Yong Jiang An-Qiang Pan State Key Laboratory of Powder Metallurgy School of Materials Science and EngineeringCentral South UniversityChangsha410083China State Key Laboratory of Separation Membranes and Membrane Processes Tianjin Key Laboratory of Advanced Fibers and Energy StorageSchool of Material Science and EngineeringTiangong UniversityTianjin300387China School of Materials Science and Engineering Hebei University of TechnologyTianjin300130China

The interfacial characteristics of the Li metal anode(LMA)play a crucial role in its overall *** various materials being applied to modify the interface,a comprehensive understanding of their specific mechanisms remains to be ***,we have prepared carbon cloth(CC)frameworks with their surfaces modified using ferromagnetic metal/LiF heterogeneous films(T^(M)-LiF-CC)as the substrate for LMA,which exhibit superior electrochemical *** ferromagnetic Co as a representative example,our study demonstrates that the enhanced performance of Co-LiF-CC,compared to bare CC,is attributed to the spinpolarized interface contributed by the Co/LiF *** and LiF play individual roles in redistributing electrons and Li^(+)to promote homogeneous Li *** nanoparticles play a crucial role in generating strong surface capacitance by storing electrons in spin-split bands,while LiF,with low surface diffusion barriers,ensures fast transportation of Li^(+).The Co-LiF-CC@Li electrodes deliver long lives of 7400 and 3600 h at 1 and 2 mA·cm^(-2)in symmetric cells,respectively;moreover,they enable full batteries with high and durable capacities,particularly when the N/P ratios are low(3.3 or even 1.7).

关键词： Lithium metal anode Interfacial charge Ferromagnetic metal Lithium fluoride Spin polarization

Expression of CC Chemokine Ligand 5 in Patients with Rheumatoid Arthritis and Its Correlation with Disease Activity and Medication

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Chinese Medical Sciences Journal 2009年第1期24卷 50-54页

作者： ming-hui yang feng-xia Wu Chuan-mei Xie Yu-feng Qing Guang-rong Wang Xiao-lan Guo Zhong Tang Jing-guo Zhou Guo-hua Yuan Institute of Rheumatology and Immunology Affiliated Hospital of North Sichuan Medical College Nanchong Sichuan 637000 China

Objective To determine the levels of CC chemokine ligand 5 (CCL5) in serum and synovial fluid (SF) from patients with rheumatoid arthritis (RA) and their relations with disease activity and medication. Methods CCL5 in serum and SF was quantified by enzyme-linked immunosorbent assay (ELISA) in 28 RA patients and 21 osteoarthritis (OA) patients. In RA patients, the correlations of CCL5 levels in serum and SF with disease activity were analyzed. Meanwhile, the serum CCL5 levels among RA patients treated with disease-modifying antirheumatic drugs (DMARDs), Tripterygium Glucosides, and other Chinese herbs without disease-modifying effects were also compared. Results CCL5 levels in both serum and SF of RA patients were significantly higher than those of OA patients (P<0.05). Moreover, the level of CCL5 was higher in SF than that in serum of RA patients (P<0.01). Serum CCL5 level was correlated significantly with the number of swollen joints (r=0.3329, P<0.05), erythrocyte sedimentation rate (r=0.4001, P<0.05), and C reactive protein (r=0.3735, P<0.01). In addition, the level of CCL5 had a trend of lower in patients treated with DMARDs or Tripterygium Glucosides than those treated with other Chinese herbs, although the difference was not significant among those patients due to the small number of patients in each group. Conclusions In RA patients, the expression of CCL5 increases and correlates with some clinical and laboratory parameters of RA, which indicate that CCL5 plays an important role in RA and may serve as a useful marker of disease activity. DMARDs and Tripterygium Glucosides might exert their clinical effects through reducing CCL5 production in RA.

关键词： rheumatoid arthritis chemokine CC chemokine ligand 5

A Study on Scalable Video Coding for AMC with Mobile Media-Based Multicast over WiMAX 802.16e

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International Journal of Communications, Network and System Sciences 2010年第4期3卷 385-394页

作者： Tzu-Kai Cheng feng-ming yang Jean-Lien C. Wu 不详

Adaptive Modulation and Coding (AMC), which is a technology used when channel condition changes, is adapted in Mobile Worldwide Interoperability for Microwave Access (WiMAX). Scalable Video Coding (SVC) is a video coding scheme used for different users with bandwidth level. SVC encodes a video into a number of layers. Users receive different number of encoded layers based on their channel condition. In this paper, Intermediate Control Server (ICS) is proposed to deal with the signaling between multimedia server and BS. Both AMC and SVC are employed to enhance the user perceived video quality in the system.

关键词： AMC SVC Server Signaling 802.16e Mobile WiMAX

Cerebrospinal fluid from rats given hypoxic preconditioning protects neurons from oxygen-glucose deprivation-induced injury

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Neural Regeneration Research 2015年第9期10卷 1471-1476页

作者： Yan-bo Zhang Zheng-dong Guo Mei-yi Li Si-jie Li Jing-zhong Niu ming-feng yang Xun-ming Ji Guo-wei Lv Department of Neurology Affiliated Hospital of Taishan Medical University Department of Endocrinology Affiliated Hospital of Taishan Medical University Department of Neurology Shandong Taishan Chronic Disease Hospital Hypoxia Medical Institute Xuanwu Hospital Capital Medical University

Hypoxic preconditioning activates endogenous mechanisms that protect against cerebral isch- emic and hypoxic injury. To better understand these protective mechanisms, adult rats were housed in a hypoxic environment （8% 02/92% N2） for 3 hours, and then in a normal oxygen environment for 12 hours. Their cerebrospinal fluid was obtained to culture cortical neurons from newborn rats for 1 day, and then the neurons were exposed to oxygen-glucose deprivation for 1.5 hours. The cerebrospinal fluid from rats subjected to hypoxic preconditioning reduced oxygen-glucose deprivation-induced injury, increased survival rate, upregulated Bcl-2 expression and downregulated Bax expression in the cultured cortical neurons, compared with control. These results indicate that cerebrospinal fluid from rats given hypoxic preconditioning protects against oxygen-glucose deprivation-induced injury by affecting apoptosis-related protein expres- sion in neurons from newborn rats.

关键词： nerve regeneration hypoxic preconditioning cerebrospinal fluich cerebral cortex oxygen-glucose deprivation neurons apoptosis Bcl-2/Bax neural regeneration

A STUDY ON Ti ELECTRODE MODIFIED BY NICKEL ION BEANS

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Chinese Chemical Letters 1991年第3期2卷 251-252页

作者： Ji Shuang ZHANG Rong Hui WANG Yao Jiao LU Han ming feng,Jin Quan yang Department of Chemistry and Chemical Engineering,Hunan University Changsha,410082

The reduction of H^+ on Ti electrodes which were treated by implanting nickel ions and thermodiffusing in vacuum has been *** calytic activity of the afore- mentioned electroes for H^+ reduction is much higher than th... 详细信息

关键词： Ti A STUDY ON Ti ELECTRODE MODIFIED BY NICKEL ION BEANS ION

Paris chinensis dioscin induces G2/M cell cycle arrest and apoptosis in human gastric cancer SGC-7901 cells

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World Journal of Gastroenterology 2011年第39期17卷 4389-4395页

作者： Lin-Lin Gao Fu-Rong Li Peng Jiao ming-feng yang Xiao-Jun Zhou Yan-Hong Si Wen-Jian Jiang Ting-Ting Zheng Department of Pathology and Pathophysiology Taishan Medical University Taian 271000 Shandong Province China School of Pharmaceutical Science Taishan Medical University Taian 271000 Shandong Province China Institute of Basic Medical Sciences Taishan Medical University Taian 271000 Shandong Province China Department of Clinical Medicine Taishan Medical University Taian 271000 Shandong Province China

AIM:To investigate the anti-tumor effects of Paris chinensis dioscin(PCD)and mechanisms regarding cell cycle regulation and apoptosis in human gastric cancer SGC-7901 ***:Cell viability was analyzed by the 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide *** apoptosis was evaluated by flow cytometry and laser scanning confocal microscope(LSCM)using Annexin-V/propidium iodide(PI)staining,and the cell cycle was evaluated using PI staining with flow *** calcium ions were detected under fluorescence *** expression of cell cycle and apoptosis-related proteins cyclin B1,CDK1,cytochrome C and caspase-3 was measured by immunohistochemical ***:PCD had an anti-proliferation effect on human gastric cancer SGC-7901 cells in a dose-and time-de-pendent *** treatment of SGC-7901 cells with PCD,apoptosis appeared in SGC-7901 ***-logical changes typical of apoptosis were also observed with LSCM by Annexin V/PI staining,and the cell number of the G0/G1 phase was decreased,while the number of cells in the G2/M phase was *** cycle-related proteins,such as cyclin B1 and CDK1,were all down-regulated,but caspase-3 and cytochrome C were ***,intracellular calcium accumulation occurred in PCD-treated ***:G2/M phase arrest and apoptosis induced by PCD are associated with the inhibition of CDK-activating kinase activity and the activation of Ca2+-related mitochondrion pathway in SGC-7901 cells.

关键词： CyclinB1/CDK1 Cell cycle arrest Caspase-3 Ca2+ Cytochrome C

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TET2基因突变在髓系肿瘤中的研究进展

中国医学科学院学报 2016年第5期38卷 583-588页

作者：李荣许明江杨逢春周圆中国医学科学院北京协和医学院血液学研究所&血液病医院实验血液学国家重点实验室天津300020

TET2为TET家族成员之一,是一个与造血系统发育密切相关的肿瘤抑制基因。近年研究发现,TET2通过将DNA上的5-甲基胞嘧啶(5-mc)氧化为5-羟甲基胞嘧啶(5-hmc),参与DNA的去甲基化过程。体细胞中TET2基因异常会导致骨髓生成异常及恶性髓系疾... 详细信息

TET2为TET家族成员之一,是一个与造血系统发育密切相关的肿瘤抑制基因。近年研究发现,TET2通过将DNA上的5-甲基胞嘧啶(5-mc)氧化为5-羟甲基胞嘧啶(5-hmc),参与DNA的去甲基化过程。体细胞中TET2基因异常会导致骨髓生成异常及恶性髓系疾病的发生。本文将就TET2的结构与功能,TET2基因突变与不同类型髓系肿瘤的关系进行综述。

关键词： TET2 突变髓系肿瘤