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Effect of Oxymatrine on the TGFbeta-Smad signaling pathway in rats with CCl_4-induced hepatic fibrosis

World Journal of Gastroenterology 2008年第13期14卷 2100-2105页

作者： Xiao-Ling Wu Wei-Zheng Zeng ming-de jiang Jian-Ping Qin Hui Xu department of Digestion General Hospital of Chengdu Military Command Chengdu 610083 Sichuan Province China

AIM： To explore the anti-fibrotic effect of Oxymatrine on CCl4-induced liver fibrosis in rats and its modulation on the TGFbeta-Smad signaling pathway. METHODS： One hundred healthy male SD rats were randomly divided into three groups： normal group （n = 20）, treatment group of Oxymatrine （n = 40） and CCh-induced fibrosis group （n = 40）. Experimental hepatic fibrosis was induced by subcutaneous injection of carbon tetrachloride （CCh soluted in liquid paraffin with the concentration of 300 g/L, the dosage of injection was 3 mL/kg, twice per week for 8 wk）. The treated rats received Oxymatrine via celiac injection at a dosage of 10 mg/kg twice a week at the same time. The deposition of collagen was observed with H＆E and Masson staining. The concentration of serum TGF-β1 was assayed with ELISA. The gene expression of Smads and CBP （CREB binding protein） was detected with in situ hybridization （ISH） and immunohistochemistry （IH）, respectively. All the experimental figures were scanned and analyzed with special figure-analysis software. RESULTS： A significant reduction of collagen deposition and rearrangement of the parenchyma was noted in the liver tissue of Oxymatrine-treated rats. The semi- quantitative histological scores （2.43 ± 0.47 μm^2 vs 3.76 ±0.68, P 〈 0.05） and average area of collagen/in those rats were significantly decreased when compared with hepatic cirrhosis model rats （94.41 ± 37.26 μm^2 vs 290.86 ± 89.37 μm^2, P 〈 0.05）. The gene expression of Smad 3 mRNA was considerably decreased in the treated animals. The A value of Smad 3 mRNA was lower in the treated rats than the model rats （0.034 ± 0.090 vs 0.167 ± 0.092, P 〈 0.05）. Contrarily, the A value of Smad 7 mRNA was increased considerably in the treated animals （0.175 ± 0.065 vs 0.074 vs 0.012, P 〈 0.05）. There was an obvious decrease in the expression of CBP mRNA in treated rats as illuminated by a reduction of its A value when compared with model rats （0.065±0.049 vs 0.235 ± 0.025, P 〈 0.001）. CONCLUSI

关键词： Oxymatrine Hepatic Fibrosis TGF-Smad signaling

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contrast enhanced computed tomography and reconstruction of hepatic vascular system for transjugular intrahepatic portal systemic shunt puncture path planning

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World Journal of Gastroenterology 2015年第32期21卷 9623-9629页

作者： Jian-Ping Qin Shan-Hong Tang ming-de jiang Qian-Wen He Hong-Bin Chen Xin Yao Wei-Zheng Zeng ming Gu department of Digestion General Hospital of Chengdu Military Command Disease Preventive and Control Center Chengdu Military Command department of Radiology General Hospital of Chengdu Military Command

AIM: To describe a method for the transjugular intrahepatic portal systemic shunt(TIPS) placement performed with the aid of contrast-enhanced computed tomography(CECT) and three-dimensional reconstructed vascular images(3D RVIs), and to assess its safety and effectiveness. METHODS: Four hundred and ninety patients were treated with TIPS between January 2005 and december 2012. All patients underwent liver CECT and reconstruction of 3D RVIs of the right hepatic vein to portal vein(PV) prior to the operation. The 3D RVIs were carefully reviewed to plan the puncture path fromthe start to target points for needle pass through the PV in the TIPS procedure. R E S U LTS :The improved TIPS procedure was successful in 483(98.6%) of the 490 patients. The number of punctures attempted was one in 294(60%) patients, 2 to 3 in 147(30%) patients, 4 to 6 in 25(5.1%) patients and more than 6 in 17(3.5%) patients. Seven patients failed. Of the 490 patients, 12 had punctures into the artery, 15 into the bile duct, eight into the gallbladder, and 18 through the liver capsule. Analysis of the portograms from the 483 successful cases indicated that the puncture points were all located distally to the PV bifurcation on anteroposterior images, while the points were located proximally to the bifurcation in the three cases with intraabdominal bleeding. The complications included three cases of bleeding, of whom one died and two needed surgery. CONCLUSION: Use of CECT and 3D RVIs to plan the puncture path for TIPS procedure is safe, simple and effective for clinical use.

关键词： Transjugular intrahepatic portal systemic shunt Co

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Clinical effects and complications of TIPS for portal hypertension due to cirrhosis:A single center

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World Journal of Gastroenterology 2013年第44期19卷 8085-8092页

作者： Jian-Ping Qin ming-de jiang Wen Tang Xiao-Ling Wu Xin Yao Wei-Zheng Zeng Hui Xu Qian-Wen He ming Gu department of Digestion Chengdu Military General Hospital department of Radiology Chengdu Military General Hospital

AIM:To determine the clinical effects and complications of transjugular intrahepatic portosystemic shunt(TIPS)for portal hypertension due to ***:Two hundred and eighty patients with portal hypertension due to cirrhosis who underwent TIPS were retrospectively *** trunk pressure was measured before and after *** changes in hemodynamics and the condition of the stent were assessed by ultrasound and the esophageal and fundic veins observed ***:The success rate of TIPS was 99.3%.The portal trunk pressure was 26.8±3.6 cmH2O after surgery and 46.5±3.4 cmH2O before surgery(Pdence of post-operative stent stenosis was 24%at 12 mo and 34%at 24 *** incidence of post-operative hepatic encephalopathy was 12%at3 mo,17%at 6 mo and 19%at 12 *** incidence of post-operative recurrent hemorrhage was 9%at 12mo,19%at 24 mo and 35%at 36 *** cumulative survival rate was 86%at 12 mo,81%at 24 mo,75%at 36 mo,57%at 48 mo and 45%at 60 ***:TIPS can effectively lower portal hypertension due to *** is significantly effective for hemorrhage of the digestive tract due to rupture of esophageal and fundic veins and for ascites and hydrothorax caused by portal hypertension.

关键词： Transjugular intrahepatic portosystemic shunt Cirrhosis Portal hypertension Therapeutic effect Complication

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Low Temperature Characteristics of FBG Temperature Sensors with Small-size Packaging

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Semiconductor Photonics and Technology 2006年第2期12卷 100-105页

作者： GUO ming-jin jiang de-sheng Key Laboratory of Fiber Optic Sensing Technology and Information Processing Wuhan University of Technology Wuhan 430070 CHN

Two small-size package methods, thin stainless steel tube based package method and plating gold based package method for fiber Bragg grating(FBG) temperature sensors, are designed. The characteristics of the FBG temperature sensors in the environment temperature between 203K and 243K are investigated. The wavelengths of the FBG temperature sensors as a function of the temperature are examined experimentally. The experiment results of the two different small-size package sensors in identical conditions are compared with each other. The transmission spectrum of the sensors is analyzed at the temperature of 273K and 213K. The temperature sensitive factors of the sensors are derived analytically and are verified by experiments. Linear fitting to the wavelength-temperature curve is carried out.

关键词： Fiber Bragg grating Low temperature Stainless steel tube package Plating gold package

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Repeated pancreatitis-induced splenic vein thrombosis leads to intractable gastric variceal bleeding: A case report and review

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World Journal of Clinical Cases 2015年第10期3卷 920-925页

作者： Shan-Hong Tang Wei-Zheng Zeng Qian-Wen He Jian-Ping Qin Xiao-Ling Wu Tao Wang Zhao Wang Xuan He Xiao-Lei Zhou Quan-Shui Fan ming-de jiang department of Digestion General Hospital of Chengdu Military Command Center for Disease Preven-tion and Control of Chengdu Military Command department of Radiology General Hospital of Chengdu Military Command department of General Surgery General Hospital of Chengdu Military Command

Gastric varices(GV) are one of the most common complications for patients with portal hypertension. Currently, histoacryl injection is recommended as the initial treatment for bleeding of GV, and this injection has been confirmed to be highly effective for most patients in many studies. However, this treatment might be ineffective for some types of GV, such as splenic vein thrombosis-related localized portal hypertension(also called left-sided, sinistral, or regional portal hypertension). Herein, we report a case of repeated pancreatitis-induced complete splenic vein thrombosis that led to intractable gastric variceal bleeding, which was treated by splenectomy. We present detailed radiological and pathological data and blood rheology analysis(the splenic artery- after a short gastric vein or stomach vein- gastric coronary vein- portal vein). The pathophysiology can be explained by the abnormal direction of blood flow in this patient. To our knowledge, this is the first reported case for which detailed patho-logy and blood rheology data are available.

关键词： Splenic vein thrombosis Intractable gastric variceal bleeding Recurrent pancreatitis Review

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sp600125对乙醛刺激的大鼠肝星状细胞凋亡及Caspase-3蛋白表达的影响

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世界华人消化杂志 2005年第18期13卷 2263-2265页

作者：唐文蒋明德李小安第三军医大学成都临床学院成都军区总医院消化内科四川省成都市610083

目的:探讨c-Jun氨基末端激酶(c-JunN-terminalkinase,JNK)信号传导通路特异阻断剂sp600125对乙醛刺激的大鼠肝星状细胞株(HSC-T6)凋亡以及Caspase-3蛋白表达的影响.方法:不同浓度sp600125对乙醛刺激的大鼠HSC-T6进行处理后,MTT比色法检... 详细信息

目的:探讨c-Jun氨基末端激酶(c-JunN-terminalkinase,JNK)信号传导通路特异阻断剂sp600125对乙醛刺激的大鼠肝星状细胞株(HSC-T6)凋亡以及Caspase-3蛋白表达的影响.方法:不同浓度sp600125对乙醛刺激的大鼠HSC-T6进行处理后,MTT比色法检测细胞增殖,用Hoechst33258染色来观察凋亡细胞的形态变化,FCM检测细胞凋亡率,SABC法检测Caspase-3蛋白表达.结果:不同浓度的sp600125能抑制HSC-T6增殖(F=102.53,P<0.01),诱导HSC-T6凋亡;随着sp600125浓度增加,HSC-T6凋亡率逐渐增高(F=38.26,P<0.01),HSC-T6细胞内Caspase-3蛋白阳性表达率也逐渐增高(F=38.26,P<0.01).结论:不同浓度sp600125能抑制HSC-T6增殖,诱导HSC-T6凋亡,这可能与促进Caspase-3蛋白表达有关.

关键词：肝纤维化肝星状细胞 sp600125 Caspase-3

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两段级联内插差异损耗功率箝制掺铒光纤放大器

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中国激光 2001年第1期28卷 63-66页

作者：王燕刘丹厉群刘小明彭江得清华大学电子工程系北京100084

针对全光网中信道路径不同所引起的功率失衡问题 ,设计了一种利用商用光纤波分复用器实现两段级联内插差异损耗环的功率箝制掺铒光纤放大器 (EDFA)光路结构 ,并从理论和实验上研究了光路结构参数对放大器动态范围和噪声特性的影响。得... 详细信息

针对全光网中信道路径不同所引起的功率失衡问题 ,设计了一种利用商用光纤波分复用器实现两段级联内插差异损耗环的功率箝制掺铒光纤放大器 (EDFA)光路结构 ,并从理论和实验上研究了光路结构参数对放大器动态范围和噪声特性的影响。得出损耗插入最佳位置在 6 0 %附近 ,最佳插入损耗大小 5～ 8dB依抽运功率和光纤长度而定。所研制的功率箝制EDFA在信号波长 15 5 3nm处 ,动态范围大于 2 0dB ,动态范围内平均输出功率超过2 90 μW。

关键词：差异损耗功率箝制光纤放大器掺铒级联内插

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deciphering fungal metabolon coupling tandem inverse-electrondemand Diels-Alder reaction and semipinacol rearrangement for the biosynthesis of spiro polycyclic alkaloids

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Science China Chemistry 2025年第1期68卷 288-296页

作者： Shuai Liu Wen-Qiang Xu Ying-Tong Di Man-Cheng Tang Ding-Kang Chen ming-ming Cao Yao-Wen Chang Hong-Yu Tang Chun-Mao Yuan Jun-Bo Yang Zhi-Li Zuo Han Guo Zi-Fei Xu Ying Zeng Yun-Dong Wu Xiao-jiang Hao Key Laboratory of Phytochemistry and Natural Medicines (Chinese Academy of Sciences) Kunming Institute of Botany Chinese Academy of Sciences University of Chinese Academy of Sciences Lab of Computational Chemistry and Drug design State Key Laboratory of Chemical Oncogenomics Peking University Shenzhen Graduate School Shenzhen Bay Laboratory State Key Laboratory of Microbial Metabolism School of Life Sciences & Biotechnology Shanghai Jiao Tong University State Key Laboratory of Functions and Applications of Medicinal Plants Guizhou Medical University Molecular Biology Experimental Center Germplasm Bank of Wild Species in Southwest China Kunming Institute of Botany Chinese Academy of Sciences Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences Research Unit of Chemical Biology of Natural Anti-Virus Products Chinese Academy of Medical Sciences

In the intricate process of natural product biosynthesis, a metabolon can enhance metabolic flux by associating sequential enzymes. A fungal metabolon, comprising of flavin-dependent monooxygenase Spe F and P450 monooxygenase Spe G, is identified in the biosynthesis of spiro polycyclic alkaloids(+)-notoamide B and its diastereomer(+)-versicolamide B. Using notoamide E as a substance, Spe F/Spe G metabolon can control the stereoselectivity of its 2,3-epoxidation, followed by hydrogen atom ion at C-17 to generate reactive epoxide tau-m A with dienyl iminium unit. Subsequently,(+)-notoamide B and(+)-versicolamide B are produced via tandem nonenzymatic inverse-electron-demand Diels-Alder reaction and semipinacol rearrangement. This provides the first example of metabolon in the biosynthesis of spiro-prenylated indole alkaloids.

关键词： metabolon Diels-Alder reaction semipinacol rearrangement biosynthesis notoamides spiro polycyclic alkaloids

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光子晶体光纤参量放大的理论模拟

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中国激光 2004年第3期31卷 310-312页

作者：倪屹王青张磊刘小明彭江得清华大学电子工程系北京100084

光子晶体光纤具有高非线性系数和非常灵活的色散特性 ,通过调节光子晶体光纤的结构参量 ,可在具有高非线性系数的同时对光子晶体光纤的零色散波长 (λ0 )进行调节。利用光子晶体光纤的这些特性可实现在所需波长上的高效率的参量放大。... 详细信息

光子晶体光纤具有高非线性系数和非常灵活的色散特性 ,通过调节光子晶体光纤的结构参量 ,可在具有高非线性系数的同时对光子晶体光纤的零色散波长 (λ0 )进行调节。利用光子晶体光纤的这些特性可实现在所需波长上的高效率的参量放大。本文采用厄密高斯函数展开的方法 ,计算了六角形光子晶体光纤 (HF)的零色散波长 ,发现当气孔间距在 1 1μm和 2 6 μm之间时 ,光子晶体光纤的零色散波长在 1 5 5 μm附近 ,并给出了零色散波长时气孔间距和气孔大小的关系曲线。对光子晶体光纤中的参量放大 (OPA)进行了理论模拟 ,计算表明在 2 0m光子晶体光纤中 ,当峰值功率为 10W时 ,参量放大的增益可达 6 0dB ,或可获得 30

关键词：光电子学光子晶体光纤色散色散斜率参量放大理论模拟

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氧化苦参碱对肝纤维化大鼠Smad基因表达的影响

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世界华人消化杂志 2005年第8期13卷 984-987页

作者：曾维政吴晓玲蒋明德成都军区总医院消化内科四川省成都市610083 重庆医科大学附一院消化内科重庆市400016

目的:研究氧化苦参碱(oxymarine,苦参素)对四氯化碳诱导的肝纤维化大鼠肝组织Smad基因表达的影响.方法:SD大鼠90只分为正常对照组(C组)、模型组(M组)和苦参素干预组(T组).CCl4sc诱导大鼠肝纤维化,干预组大鼠在造模的同时给予苦参素注射... 详细信息

目的:研究氧化苦参碱(oxymarine,苦参素)对四氯化碳诱导的肝纤维化大鼠肝组织Smad基因表达的影响.方法:SD大鼠90只分为正常对照组(C组)、模型组(M组)和苦参素干预组(T组).CCl4sc诱导大鼠肝纤维化,干预组大鼠在造模的同时给予苦参素注射液ip,正常对照组给予液体石蜡sc和生理盐水ip,8wk实验结束时处死动物.HE和Masson染色观察大鼠肝组织病理学变化和胶原沉积,原位杂交和免疫组化检测Smad4,Smad7基因表达情况.结果:氧化苦参碱干预组大鼠肝半定量组织学积分(1.9±0.3vs3.6±0.8,P<0.05)和胶原面积(94±37vs691±189μm2,P<0.05)明显减少;Smad7蛋白表达阳性率增加[(5.09±0.68)%vs(2.86±0.865%,P<0.05];Smad4蛋白表达阳性率减少[(2.33±1.64)%vs(9.56±1.34)%,P<0.05];Smad4mRNA表达阳性率显著减少(0.31±0.12vs0.62±0.23,P<0.05);Smad7mRNA的表达则明显增加(0.26±0.11vs0.16±0.03,P<0.05).结论:氧化苦参碱能抑制Smad4基因表达,促进Smad7基因表达.

关键词：氧化苦参碱肝纤维化大鼠 Smad 基因表达

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