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Reperfusion after hypoxia-ischemia exacerbates brain injury with compensatory activation of the antiferroptosis system:based on a novel rat model

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Neural Regeneration Research 2023年第10期18卷 2229-2236页

作者： Tian-Lei Zhang Zhi-Wei Zhang Wei Lin Xin-Ru Lin Ke-Xin Lin Ming-Chu Fang Jiang-Hu Zhu Xiao-Ling Guo Zhen-Lang Lin Department of Pediatrics the Second School of Medicinethe Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina Second Clinical Medical College Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina Key Laboratory of Children Genitourinary Diseases of Wenzhou the Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina Key Laboratory of Perinatal Medicine of Wenzhou the Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina Basic Medical Research Center the Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina

Hypoxic-ischemic encephalopathy,which predisposes to neonatal death and neurological sequelae,has a high morbidity,but there is still a lack of effective prevention and treatment in clinical practice.To better understand the pathophysiological mechanism underlying hypoxic-ischemic encephalopathy,in this study we compared hypoxic-ischemic reperfusion brain injury and simple hypoxic-ischemic brain injury in neonatal rats.First,based on the conventional RiceVannucci model of hypoxic-ischemic encephalopathy,we established a rat model of hypoxic-ischemic reperfusion brain injury by creating a common carotid artery muscle bridge.Then we performed tandem mass tag-based proteomic analysis to identify differentially expressed proteins between the hypoxic-ischemic reperfusion brain injury model and the conventional Rice-Vannucci model and found that the majority were mitochondrial proteins.We also performed transmission electron microscopy and found typical characteristics of ferroptosis,including mitochondrial shrinkage,ruptured mitochondrial membranes,and reduced or absent mitochondrial cristae.Further,both rat models showed high levels of glial fibrillary acidic protein and low levels of myelin basic protein,which are biological indicators of hypoxic-ischemic brain injury and indicate similar degrees of damage.Finally,we found that ferroptosis-related Ferritin(Fth1)and glutathione peroxidase 4 were expressed at higher levels in the brain tissue of rats with hypoxic-ischemic reperfusion brain injury than in rats with simple hypoxic-ischemic brain injury.Based on these results,it appears that the rat model of hypoxic-ischemic reperfusion brain injury is more closely related to the pathophysiology of clinical reperfusion.Reperfusion not only aggravates hypoxic-ischemic brain injury but also activates the anti-ferroptosis system.

关键词： ferroptosis hypoxic-ischemic brain injury hypoxic-ischemic encephalopathy hypoxic-ischemic reperfusion brain injury mitochondria model proteomic analysis reperfusion Rice-Vannucci transmission electron microscopy

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Reliability of Tonolab measurements in rats

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International Journal of Ophthalmology(English edition) 2014年第6期7卷 930-934页

作者： Li-fang Liu chu-Kai Huang ming-Zhi Zhang Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong

AIM: To assess the repeatability and reproducibility of Tonolab tonometer in rats with high intraocular pressure(IOP) and evaluate its ability to detect IOP changes in rats with general anaesthesia.METHODS: Left eyes of adult Fischer rats(F344) were photocoagulated by 532 nm diode laser to induce high IOP. Hypertensive eyes of 30 conscious rats were randomly chosen to measure IOP on a single occasion.Two observers independently and alternately undertook IOP measurements consecutively for three times using the same Tonolab tonometer blind to the other observer’s IOP measurements. The within subject standard deviation(Sw), coefficient of variation(CVw)(100 ×Sw/overall mean), and intraclass correlation coefficient(ICC) were calculated to evaluate intra-observer repeatability. Inter-observer difference was analysed by using 95% limits of agreement described by Bland-Altman and paired sample t- test. Also, another13 normal F344 rats were intraperitoneally administrated with ketamine/xylazine or chloral hydrate, and IOPs of both eyes were measured by a single operator once every 5min until animals came to conscious. IOPs at various time points were compared by using one-way ANOVAs.RESULTS: Mean IOP was 35.58 mm Hg(range 17.33 to65.33 mm Hg). For intraobserver repeatability, the Sw,CVw and ICC of high IOP for two observers were 5.20 mm Hg/3.41 mm Hg, 9.98% /8.08% and 0.820/0.928 respectively.The inter-observer difference was 14.76% ±19.76% of the mean IOP of two observers, with a 95% limits of agreement-23.97% to 53.50%, and the difference between mean IOP of these two observers was statistically significant(P =0.001). IOPs dropped slightly during the first 15 min post-aneathesia, with a IOP change between0.17 and 1.17 mm Hg. IOPs changed from basline of11.75 ±2.05 mm Hg(n =12) to 8.75 ±1.06 mm Hg 20 min post-anesthesia(P =0.001), and this hypotensivecondition persisted until 80 min post-anesthesia. CONCLUSION: In this sample of hypertensive rats,Tonolab measurements demonstrated high levels of intraobserver repeatabili

关键词： Tonolab repeatability reproducibility rats

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Mineral and Bone Disorder and Its Association with Cardiovascular Parameters in Chinese Patients with Chronic Kidney Disease

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Chinese Medical Journal 2016年第19期129卷 2275-2280页

作者： chu Zhou fang Wang Jin-Wei Wang Lu-Xia Zhang ming-Hui Zhao Renal Division Department of Medicine Peking University First Hospital Peking University Institute of Nephrology Key Laboratory of Renal DiseaseMinistry of Health of China Key Laboratory of ChronicKidney Disease Prevention and Treatment (Peking University) Ministry of Education Beijing 100034 China Guizhou Medical University Guiyang Guizhou 550004 China

Background：Mineral and bone disorder （MBD）,especially hyperphosphatemia,is an independently risk factor for adverse prognosis in patients with chronic kidney disease （CKD）.However,CKD-MBD among Chinese population was poorly studied.This study aimed to investigate the status of MBD and its association with cardiovascular parameters in Chinese patients with predialysis CKD.Methods：Chinese Cohort Study of Chronic Kidney Disease （C-STRIDE） is a prospective multicenter cohort study involving predialysis CKD patients in China.Markers of MBD,including serum phosphorus,calcium,and intact parathyroid hormone,were measured in baseline samples at the patients＆#39; entry.The association between serum phosphorus and abdominal aortic calcification （AAC）,left ventricular hypertrophy （LVH） were examined by logistic regression models.Results：Altogether 3194 predialysis patients with mean estimated glomerular filtration of 51.8 ± 33.1 ml·min^- 1· 1.73 m^- 2 were included.The proportion of patients with hyperphosphatemia were 2.6%,2.9%,6.8%,and 27.1% in CKD Stages 3a,3b,4,and 5,respectively.Moreover,71.6% of the patients with hyperphosphatemia did not receive any phosphate-binder （PB）.Lateral abdominal X-rays were obtained in 2280 patients,9.8% of the patients were diagnosed as having AAC.Altogether 2219 patients had data of echocardiography,and 13.2% of them were diagnosed with LVH.Multivariate logistic regression analysis showed that serum phosphorus was independently associated with the presence of AAC and LVH.Conclusions：In Chinese patients with CKD,the percentage of hyperphosphatemia is comparable to that of other countries while the usage of PBs is suboptimal.The prevalence of vascular calcification in Chinese patients is relatively lower compared with the Caucasian population.

关键词： Chronic Kidney Disease Hyperphosphatemia Left Ventricular Hypertrophy Mineral and Bone Disorder Vascular Calcification

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Hyper-crosslinked Conjugated Microporous Polymers with Increased Micropores Promotes Confining Polymerization for Electromagnetic Absorption Application

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Chinese Journal of Polymer Science 2023年第8期41卷 1305-1316,I0011页

作者： Hao Chen fang-Yuan chu Hai-Yan Zhuang Lu Wang Zhi-Min Ye Wei Dong Zhi-Peng Xie A-ming Xie School of Mechanical Engineering Nanjing University of Science&TechnologyNanjing 210094China State Key Laboratory for Marine Corrosion and Protection Luoyang Ship Material Research Institute(LSMRI)Xiamen 361100China School of Chemistry and Chemical Engineering Nanjing University of Science&TechnologyNanjing 210094China

Conjugated microporous polymers have excellent skeleton structures but poor electrical conductivity limits their applications in microwave absorption.To solve this problem,a strategy of molecular expansion and confining polymerization is proposed in this work to synthesize conductive hyper-crosslinked conjugated microporous polymer.The topology of the conjugated microporous polymer is changed into a three-dimensional skeleton structure with high specific surface area by using molecular expansion technique,and the controlled growth of polypyrrole in the channel constructs a unique network structure.The balance of excellent composite backbone structure,proper conductivity,attenuation capability and impedance matching enable the material to exhibit electromagnetic wave absorption performance.As a result,with low filler loading of 10 wt%,a strongest reflection loss of-52.68 d B and a maximum effective bandwidth of 5.76 GHz.Additionally,CST simulations of the radar scattering cross section have been carried out to verify the excellent material properties.This study provides new concepts for new conductive polymers and broadens the application of hyper-crosslinked conjugated microporous polymer in the field of electromagnetic wave absorption.

关键词： Hyper-crosslinked conjugated microporous polymer Electromagnetic wave absorbing Molecular expansion Polypyrrole

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Yang cycle enzyme DEP1: its moonlighting functions in PSI and ROS production during leaf senescence

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Molecular Horticulture 2022年第1期2卷 133-148页

作者： chu-Kun Wang Xiu-ming Li fang Dong Cui-Hui Sun Wen-Li Lu Da-Gang Hu National Key Laboratory of Crop Biology MOA Key Laboratory of Horticultural Crop Biology and Germplasm InnovationCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai’an 271018ShandongChina Shandong Institute of Pomology Key Laboratory for Fruit Biotechnology Breeding of ShandongTai’an 271000ShandongChina

Ethylene-mediated leaf senescence and the compromise of photosynthesis are closely associated but the underlying molecular mechanism is a mystery.Here we reported that apple DEHYDRATASE-ENOLASE-PHOSPHATASE-COMPLEX1(MdDEP1),initially characterized to its enzymatic function in the recycling of the ethylene precursor SAM,plays a role in the regulation of photosystem I(PSI)activity,activating reactive oxygen species(ROS)homeostasis,and negatively regulating the leaf senescence.A series of Y2H,Pull-down,CO-IP and Cell-free degradation biochemical assays showed that MdDEP1 directly interacts with and dephosphorylates the nucleus-encoded thylakoid protein MdY3IP1,leading to the destabilization of MdY3IP1,reduction of the PSI activity,and the overproduction of ROS in plant cells.These findings elucidate a novel mechanism that the two pathways intersect at MdDEP1 due to its moonlighting role in destabilizing MdY3IP1,and synchronize ethylene-mediated leaf senescence and the compromise of photosynthesis.

关键词： DEHYDRATASE-ENOLASE-PHOSPHATASE-COMPLEX1 Yang cycle Photosystem I ROS Leaf senescence Photosynthesis

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Coexpression of TLR9 and VEGF-C is associated with lymphatic metastasis in prostate cancer

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Asian Journal of Andrology 2022年第4期24卷 380-385页

作者： Xian-Zi Zeng Zhan-Sen Huang Hong-Peng fang Jie-Ying Wu Qun-Xiong Huang chu-Bin Zhuang Jing Zhou Jin-ming Di Department of Urology The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630China Department of Infertility and Sexual Medicine The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630China Department of Pathology The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630China

Prostate cancer(PCa)is one of the most frequent cancers in men,and its biomolecular targets have been extensively studied.This study aimed to analyze the expression of toll-like receptor 9(TLR9)and vascular endothelial growth factor C(VEGF-C)and the clinical value of the coexpression of TLR9 and VEGF-C in PCa.We retrospectively evaluated 55 patients with clinically localized,intermediate-risk,or high-risk PCa who underwent laparoscopic radical prostatectomy(LRP)and extended pelvic lymph node dissection(ePLND)without neoadjuvant hormonal therapy at a single institution from June 2013 to December 2016.In all 55 patients,the median number of lymph nodes(LNs)resected was 23(range:18-31),and a total of 1269 LNs were removed,of which 78 LNs were positive.Seventeen patients had positive LNs,with a positive rate of 30.9%.In addition,the immunohistochemical results in the above patients revealed that high TLR9 expression was correlated with higher Gleason score(GS)(P=0.049),increased LN metastasis(P=0.004),and more perineural invasion(PNI)(P=0.033).Moreover,VEGF-C expression was associated with GS(P=0.040),pathological stage(pT stage)(P=0.022),LN metastasis(P=0.003),and PNI(P=0.001).Furthermore,a significant positive correlation between TLR9 and VEGF-C was found(P<0.001),and the TLR9/VEGF-C phenotype was associated with LN metastasis(P=0.047).Collectively,we propose that TLR9 stimulation may promote LN metastasis in PCa cells through the upregulation of VEGF-C expression,thereby affecting the prognosis of PCa patients.Therefore,these markers may serve as valuable targets for the treatment of PCa.

关键词： biochemical progression-free survival coexpression lymphatic metastasis prostate cancer toll-like receptor 9 vascular endothelial growth factor C

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Antibacterial mechanism and transcriptomic analysis of a nearinfrared triggered upconversion nanoparticles@AgBiS2 for synergetic bacteria-infected therapy

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Nano Research 2022年第10期15卷 9298-9308页

作者： Shi Chen Zhaoyou chu Limian Cao Lingling Xu Qianqian Jin Nian Liu Benjin Chen ming fang Wanni Wang Haisheng Qian Min Shao Department of Critical Care Medicine The First Affiliated Hospital of Anhui Medical UniversityHefei 230032China School of Biomedical Engineering Research and Engineering Center of Biomedical MaterialsAnhui Provincial Institute of Translational MedicineAnhui Medical UniversityHefei 230032China

Methicillin-resistant Staphylococcus aureus(MRSA)has become a rising clinical problem as its occurrence has increased due to the overuse and misuse of antibiotics.In this work,upconversion nanoparticles@AgBiS2 core–shell were produced with enhanced photothermal transformation efficiency and ability to produce reactive oxygen species for synergistic photodynamic photothermal and photodynamic antibacterial performance.The nanoparticles exhibit good antibacterial effects in vitro and satisfactory therapeutic performance on healing MRSA-infected wounds in vivo experiments.RNA-sequencing technique has been used to investigate and reveal that photothermal–photodynamic therapy using the nanoparticles can interfere with metabolic processes such as galactose metabolism in MRSA bacteria,destroy the transport system on the surface of MRSA,and affect quorum sensing to hinder the formation of biofilms to achieve effective antibacterial efficacy.It was demonstrated that this work presents an alternative near-infrared photoactive multimodal nanostructure for antibacterial applications.

关键词： photothermal therapy photodynamic therapy anti-methicillin-resistant Staphylococcus aureus(MRSA) RNA-sequencing transcriptomic analysis

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治理吸纳执法:乡镇行政执法何以运行——基于中部多个乡镇综合执法改革的经验考察

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华中科技大学学报（社会科学版） 2024年第3期38卷 95-105页

作者：纪芳褚明浩武汉大学社会学院中国地质大学(武汉)公共管理学院

近年来国家不断强调治理重心下移,行政执法权下沉是其重要表现。以执法下沉为目标的行政执法改革旨在通过行政执法权的属地化配置缓解乡镇治理权责失衡的困境,促进基层治理规范化。但实践中,乡镇行政执法形成“治理吸纳执法”的运行特征... 详细信息

近年来国家不断强调治理重心下移,行政执法权下沉是其重要表现。以执法下沉为目标的行政执法改革旨在通过行政执法权的属地化配置缓解乡镇治理权责失衡的困境,促进基层治理规范化。但实践中,乡镇行政执法形成“治理吸纳执法”的运行特征,即行政执法作为“条条”体制下的一种专业化事权呈现出“块块”主导的属地化运作,这源于乡村社会的弱执法需求以及伴随多中心工作而来的乡镇治权弱化。行政执法下沉客观上为乡镇提供了新的治理工具,但并未带来预期的执法效益,也没有根本改变乡镇治权不足的结构性困境。乡镇治理权力具有多样性和复杂性,基于乡镇治理情景,具体分析行政执法权的性质,才能正确理解行政执法权在乡村治理中的功能定位以及行政执法改革对乡镇治理的意义。

关键词：乡镇治理行政执法改革综合执法治理吸纳执法乡镇治权

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Breaking the barriers: advances in acoustic functional materials

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National Science Review 2018年第2期5卷 159-182页

作者： Hao Ge Min Yang chu Ma ming-Hui Lu Yan-Feng Chen Nicholas fang Ping Sheng National Laboratory of Solid State Microstructures and Department of Materials Science and Engineering College of Engineering and Applied SciencesNanjing University Department of Physics Hong Kong University of Science and Technology Department of Mechanical Engineering Massachusetts Institute of Technology Collaborative Innovation Center of Advanced Microstructures Nanjing University

Acoustics is a classical field of study that has witnessed tremendous developments over the past 25 years.Driven by the novel acoustic effects underpinned by phononic crystals with periodic modulation of elastic building blocks in wavelength scale and acoustic metamaterials with localized resonant units in subwavelength scale, researchers in diverse disciplines of physics, mathematics, and engineering have pushed the boundary of possibilities beyond those long held as unbreakable limits. More recently, structure designs guided by the physics of graphene and topological electronic states of matter have further broadened the whole field of acoustic metamaterials by phenomena that reproduce the quantum effects classically. Use of active energy-gain components, directed by the parity–time reversal symmetry principle,has led to some previously unexpected wave characteristics. It is the intention of this review to trace historically these exciting developments, substantiated by brief accounts of the salient milestones. The latter can include, but are not limited to, zero/negative refraction, subwavelength imaging, sound cloaking, total sound absorption, metasurface and phase engineering, Dirac physics and topology-inspired acoustic engineering, non-Hermitian parity–time synthetic active metamaterials, and one-way propagation of sound waves. These developments may underpin the next generation of acoustic materials and devices, and offer new methods for sound manipulation, leading to exciting applications in noise reduction, imaging, sensing and navigation, as well as communications.

关键词： acoustic metamaterials phononic crystals topological acoustics PT-symmetry synthetic acoustics sound absorption

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Anti-fibrotic effect of rosmarinic acid on inhibition of pterygium epithelial cells

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International Journal of Ophthalmology(English edition) 2018年第2期11卷 189-195页

作者： Ya-Yu Chen Chia-fang Tsai ming-chu Tsai Wen-Kang Chen Yu-Wen Hsu Fung-Jou Lu Institute of Medicine College of MedicineChung Shan Medical UniversityTaichung City 40201TaiwanChina Department of Biotechnology TransWorld UniversityDouliu City 64063TaiwanChina Department of Applied Cosmetology Tainan Junior College of NursingTainan City 70043TaiwanChina Department of Optometry Da-Yeh UniversityChanghua 51591TaiwanChina Department of Ophthalmology Chung Shan Medical University HospitalTaichung City 40201TaiwanChina

AIM： To investigate the anti-fibrosis effect of rosmarinic acid（RA） in pterygium epithelial cells（PECs） to determine if RA is a potent agent for treating pterygium.METHODS： The PECs（1×10-4 cells/mL） were treated with 100 μmol/L of RA for 1, 3 and 6h. After RA treatment, the cell viability was determined by staining with acridine orange/DAPI and analysis via a NucleoC ounter NC-3000. The protein expression levels of type I collagen, transforming growth factor beta-1（TGF-β1）, TGF-β type Ⅱ receptor（TGF-βRⅡ）, p-Smad1/5, p-Smad2, p-Smad3, and Smad4 of the cell lysates were measured by Western blot analysis.RESULTS： The cell viability of PECs was significantly decreased after RA treatment（P〈0.01）. As the result, RA reduced the protein expression of typeⅠcollagen and TGF-β1 of PECs. Additionally, RA also inhibited TGF-β1/Smad signaling by decreasing the protein expressions of TGF-βRII, p-Smad1/5, p-Smad2, p-Smad3, and Smad4.CONCLUSION： This study demonstrate that RA could inhibit fibrosis of PECs by down-regulating type I collagen expression and TGF-β1/Smad signaling. Therefore, RA is a potent therapeutic agent for the treatment of pterygium.

关键词： fibrosis pterygium rosmarinic acid transforming growth factor beta-1 typeⅠcollagen

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