检索结果-南通市图书馆

Traditional Medicine Research 2024年第9期9卷 21-31页

作者： Han-Qing Zhang Shuang Wu Xin Chen Ya-Xuan Fang Qiu-Mei Lan Zi-Jun Zhou Yan-Heng Qiao Jie Li Yan-Ru Zhao ming pei Bo Yang Department of Nephropathy First Teaching Hospital of Tianjin University of Traditional Chinese MedicineTianjin 300381China National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion Tianjin 300381China

Vascular calcification is a crucial risk factor that affects the incidence and mortality of cardiovascular disease in chronic kidney disease patients.Modern medicine relies on calcium-phosphorus binding agents,calcium mimetics,active vitamin D,and hemodialysis to prevent and treat vascular calcification,however,their efficacy is unsatisfactory and adverse reactions often occur.Medical plant therapy can act as an integrative regulator in patients with chronic kidney disease-associated vascular calcification,which can significantly improve patients’symptoms,but its specific mechanism has not been fully elucidated yet.In this paper,we reviewed the domestic and international theoretical studies on the pathogenesis mechanism of chronic kidney disease-associated vascular calcification in recent years,summarized eight active ingredients of medicinal plants as well as four compound formulas for improving chronic kidney disease-associated vascular calcification,and explored the mechanism of action of herbal medicine,which will provide a new strategy for promoting the prevention and treatment of vascular calcification.

关键词： chronic kidney disease chronic kidney disease-mineral and bone disorder(CKD-MBD) vascular calcification medicinal plants herbal monomers

Decellularized extracellular matrix mediates tissue construction and regeneration

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Medicine 2022年第1期16卷 56-82页

作者： Chuanqi Liu ming pei Qingfeng Li Yuanyuan Zhang Department of Plastic and Burn Surgery West China HospitalSichuan UniversityChengdu 610041China Department of Plastic and Reconstructive Surgery Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China Stem Cell and Tissue Engineering Laboratory Department of OrthopaedicsWest Virginia UniversityMorgantownWV 26506USA Wake Forest Institute for Regenerative Medicine Wake Forest University Health SciencesWinston-SalemNC 27109USA

Contributing to organ formation and tissue regeneration,extracellular matrix(ECM)constituents provide tissue with three-dimensional(3D)structural integrity and cellular-function regulation.Containing the crucial traits of the cellular microenvironment,ECM substitutes mediate cell–matrix interactions to prompt stem-cell proliferation and differentiation for 3D organoid construction in vitro or tissue regeneration in vivo.However,these ECMs are often applied generically and have yet to be extensively developed for specific cell types in 3D cultures.Cultured cells also produce rich ECM,particularly stromal cells.Cellular ECM improves 3D culture development in vitro and tissue remodeling during wound healing after implantation into the host as well.Gaining better insight into ECM derived from either tissue or cells that regulate 3D tissue reconstruction or organ regeneration helps us to select,produce,and implant the most suitable ECM and thus promote 3D organoid culture and tissue remodeling for in vivo regeneration.Overall,the decellularization methodologies and tissue/cell-derived ECM as scaffolds or cellular-growth supplements used in cell propagation and differentiation for 3D tissue culture in vitro are discussed.Moreover,current preclinical applications by which ECM components modulate the wound-healing process are reviewed.

关键词： decellularized extracellular matrix 3D culture organoids tissue repair

Nidogen:A matrix protein with potential roles in musculoskeletal tissue regeneration

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2022年第3期9卷 598-609页

作者： Sheng Zhou Song Chen Yixuan Amy pei ming pei Stem Cell and Tissue Engineering Laboratory Department of OrthopaedicsWest Virginia UniversityMorgantownWV 26506USA Department of Orthopaedics The GeneraLHospital of Western Theater CommandChengduSichuan 610083PR China WVU Cancer Institute Robert C.Byrd Health Sciences CenterWest Virginia UniversityMorgantownWV 26506USA

Basement membrane proteins are known to guide cell structures,differentiation,and tissue repair.Although there is a wealth of knowledge on the functions of laminins,per-lecan,and type IV collagen in maintaining tissue homeostasis,not much is known about nidogen.As a key molecule in the basement membrane,nidogen contributes to the formation of a delicate microenvironment that proves necessary for stem cell lineage-specific differentiation.In this review,the expression of nidogen is delineated at both cellular and tissue levels from embryonic to adult stages of development;the effect of nidogens is also summarized in the context of musculoskeletal development and regeneration,including but not limited to adipogenesis,angiogenesis,chondrogenesis,myogenesis,and neurogenesis.Furthermore,potential mechanisms underlying the role of nidogens in stem cell-based tissue regeneration are also discussed.This concise review is expected to facilitate our existing understanding and utilization of nidogen in tissue engineering and regeneration.

关键词： Adipose Basement membrane Cartilage Differentiation Nerve Nidogens Vessel

Synchronization and firing mode transition of two neurons in a bilateral auditory system driven by a high–low frequency signal

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Physics B 2024年第5期33卷 722-735页

作者： Charles Omotomide Apata 唐浥瑞周祎凡蒋龙裴启明 School of Physics and Optoelectronic Engineering Yangtze UniversityJingzhou 434023China

The FitzHugh–Nagumo neuron circuit integrates a piezoelectric ceramic to form a piezoelectric sensing neuron,which can capture external sound signals and simulate the auditory neuron system.Two piezoelectric sensing neurons are coupled by a parallel circuit consisting of a Josephson junction and a linear resistor,and a binaural auditory system is established.Considering the non-singleness of external sound sources,the high–low frequency signal is used as the input signal to study the firing mode transition and synchronization of this system.It is found that the angular frequency of the high–low frequency signal is a key factor in determining whether the dynamic behaviors of two coupled neurons are synchronous.When they are in synchronization at a specific angular frequency,the changes in physical parameters of the input signal and the coupling strength between them will not destroy their synchronization.In addition,the firing mode of two coupled auditory neurons in synchronization is affected by the characteristic parameters of the high–low frequency signal rather than the coupling strength.The asynchronous dynamic behavior and variations in firing modes will harm the auditory system.These findings could help determine the causes of hearing loss and devise functional assistive devices for patients.

关键词： piezoelectric ceramic Josephson junction auditory neuron synchronization

Matrix from urine stem cells boosts tissue-specific stem cell mediated functional cartilage reconstruction

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bioactive Materials 2023年第5期23卷 353-367页

作者： ming pei Yixuan Amy pei Sheng Zhou Elmira Mikaeiliagah Christopher Erickson Benjamin Giertych Halima Akhter Lei Wang Amanda Stewart Joshua Parenti Bin Wang Sijin Wen Sotcheadt Sim Eric Quenneville Kirk C.Hansen Steven Frisch Gangqing Hu Stem Cell and Tissue Engineering Laboratory Department of OrthopaedicsWest Virginia UniversityMorgantownWVUSA WVU Cancer Institute Robert C.Byrd Health Sciences CenterWest Virginia UniversityMorgantownWVUSA Perelman School of Medicine University of PennsylvaniaPhiladelphiaPAUSA Department of Biology Ardabil BranchIslamic Azad UniversityArdabilIran Department of Biochemistry&Molecular Genetics University of Colorado DenverAuroraCOUSA Department of Microbiology Immunologyand Cell BiologyWest Virginia UniversityMorgantownWVUSA Department of Foot and Hand Surgery Clinical Medical College of Yangzhou UniversitySubei People’s Hospital of Jiangsu ProvinceYangzhouJiangsuChina Department of Biostatistics School of Public HealthWest Virginia UniversityMorgantownWVUSA Biomomentum Inc. LavalQuebecCanada Department of Biochemistry West Virginia UniversityMorgantownWVUSA Bioinformatics Core West Virginia UniversityMorgantownWVUSA

Articular cartilage has a limited capacity to self-heal once damaged.Tissue-specific stem cells are a solution for cartilage regeneration;however,ex vivo expansion resulting in cell senescence remains a challenge as a large quantity of high-quality tissue-specific stem cells are needed for cartilage regeneration.Our previous report demonstrated that decellularized extracellular matrix(dECM)deposited by human synovium-derived stem cells(SDSCs),adipose-derived stem cells(ADSCs),urine-derived stem cells(UDSCs),or dermal fibroblasts(DFs)provided an ex vivo solution to rejuvenate human SDSCs in proliferation and chondrogenic potential,particularly for dECM deposited by UDSCs.To make the cell-derived dECM(C-dECM)approach applicable clinically,in this study,we evaluated ex vivo rejuvenation of rabbit infrapatellar fat pad-derived stem cells(IPFSCs),an easily accessible alternative for SDSCs,by the abovementioned C-dECMs,in vivo application for functional cartilage repair in a rabbit osteochondral defect model,and potential cellular and molecular mechanisms underlying this rejuvenation.We found that C-dECM rejuvenation promoted rabbit IPFSCs’cartilage engineering and functional regeneration in both ex vivo and in vivo models,particularly for the dECM deposited by UDSCs,which was further confirmed by proteomics data.RNA-Seq analysis indicated that both mesenchymal-epithelial transition(MET)and inflammation-mediated macrophage activation and polarization are potentially involved in the C-dECM-mediated promotion of IPFSCs’chondrogenic capacity,which needs further investigation.

关键词： Decellularized extracellular matrix Tissue-specific stem cell Urine-derived stem cell Rejuvenation Functional cartilage repair

Chondrogenic priming of human fetal synovium-derived stem cells in an adult stem cell matrix microenvironment

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2015年第4期2卷 337-346页

作者： Jingting Li Fan He ming pei Stem Cell and Tissue Engineering Laboratory Department of OrthopaedicsWest Virginia UniversityMorgantownWV 26506USA Division of Exercise Physiology West Virginia UniversityMorgantownWV 26506USA Orthopaedic Institute Soochow UniversitySuzhou 215007China

Cartilage defects are a challenge to treat clinically due to the avascular nature of cartilage.Low immunogenicity and extensive proliferation and multidifferentiation potential make fetal stem cells a promising source for regenerative medicine.In this study,we aimed to determine whether fetal synovium-derived stem cells(FSDSCs)exhibited replicative senescence and whether expansion on decellularized extracellular matrix(dECM)deposited by adult SDSCs(AECM)promoted FSDSCs’chondrogenic potential.FSDSCs from passage 2 and 9 were compared for chondrogenic potential,using Alcian blue staining for sulfated glycosaminoglycans(GAGs),biochemical analysis for DNA and GAG amounts,and real-time PCR for chondrogenic genes including ACAN and COL2A1.Passage 3 FSDSCs were expanded for one passage on plastic flasks(PL),AECM,or dECM deposited by fetal SDSCs(FECM).During expansion,cell proliferation was evaluated using flow cytometry for proliferation index,stem cell surface markers,and resistance to hydrogen peroxide.During chondrogenic induction,expanded FSDSCs were evaluated for tri-lineage differentiation capacity.We found that cell expansion enhanced FSDSCs’chondrogenic potential at least up to passage 9.Expansion on dECMs promoted FSDSCs’proliferative and survival capacity and adipogenic differentiation but not osteogenic capacity.AECM-primed FSDSCs exhibited an enhanced chondrogenic potential.

关键词： Chondrogenesis Decellularized extracellular matrix Fetal stem cell In vitro microenvironment Replicative senescence Synovium-derived stem cell

Strategies to minimize hypertrophy in cartilage engineering and regeneration

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2015年第1期2卷 76-95页

作者： Song Chen peiliang Fu Ruijun Cong HaiShan Wu ming pei Stem Cell and Tissue Engineering Laboratory Department of OrthopaedicsWest Virginia UniversityMorgantownWV 26506USA Department of Joint Surgery Shanghai Changzheng HospitalThe Second Military Medical UniversityShanghai 200003China Department of Orthopaedics The 10th People’s Hospital of ShanghaiAffiliated with Tongji UniversityShanghai 200072China Exercise Physiology West Virginia UniversityMorgantownWV 26506USA Mechanical and Aerospace Engineering West Virginia UniversityMorgantownWV 26506USA 不详

Due to a blood supply shortage,articular cartilage has a limited capacity for selfhealing once damaged.Articular chondrocytes,cartilage progenitor cells,embryonic stem cells,and mesenchymal stem cells are candidate cells for cartilage regeneration.Significant current attention is paid to improving chondrogenic differentiation capacity;unfortunately,the potential chondrogenic hypertrophy of differentiated cells is largely overlooked.Consequently,the engineered tissue is actually a transient cartilage rather than a permanent one.The development of hypertrophic cartilage ends with the onset of endochondral bone formation which has inferior mechanical properties.In this review,current strategies for inhibition of chondrogenic hypertrophy are comprehensively summarized;the impact of cell source options is discussed;and potential mechanisms underlying these strategies are also categorized.This paper aims to provide guidelines for the prevention of hypertrophy in the regeneration of cartilage tissue.This knowledge may also facilitate the retardation of osteophytes in the treatment of osteoarthritis.

关键词： Adult stem cell Cartilage regeneration Cartilage repair Cartilage tissue engineering Chondrogenesis Hypertrophy

计及频率响应时空相关性的新能源电力系统惯量估计方法

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

电力系统自动化 2024年第8期48卷 53-66页

作者：裴铭叶林罗雅迪沙立成张再驰宋旭日中国农业大学信息与电气工程学院北京市100083 中国电力科学研究院有限公司北京市100192 国网北京市电力公司北京市100031 国网北京市电力公司电力科学研究院北京市100075

高比例新能源电力系统惯性水平低、不确定性强,系统惯性水平与频率动态响应过程密切相关。系统内各节点之间的频率响应过程在时空上具有相关性,动态量化节点间频率的时空相关性是实时估计新型电力系统惯量的技术手段。首先,给出了面向... 详细信息

高比例新能源电力系统惯性水平低、不确定性强,系统惯性水平与频率动态响应过程密切相关。系统内各节点之间的频率响应过程在时空上具有相关性,动态量化节点间频率的时空相关性是实时估计新型电力系统惯量的技术手段。首先,给出了面向同步机组和新能源发电机组惯量的计算方法;其次,利用Granger因果检验算法,动态分析系统内不同节点之间频率的相关性,构建随时间变化的系统频率时空因果相关集合;此外,基于系统内各节点频率响应过程的摇摆方程,建立系统惯性常数-频率状态空间模型;然后,基于无迹卡尔曼滤波器和固定滞后平滑器,提出高比例新能源电力系统惯量估计方法;最后,在改进的IEEE 39节点系统中验证了所提方法的有效性和适用性。结果表明,所提方法可以在不同新能源渗透率和频率扰动场景下保证惯量估计效果的一致性,具有工程应用潜力。

关键词：惯量估计电力系统新能源因果相关状态空间频率响应滤波-平滑两阶段估计

装配式钢筋混凝土梁柱螺栓连接节点抗震性能试验

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

工程抗震与加固改造 2024年第3期46卷 72-80页

作者：吴东平裴明晓李成玉武汉科技大学城市建设学院湖北武汉430065

提出了一种干式装配式梁柱节点,该节点由核心区梁端外包钢套筒与柱预留孔洞实现螺栓连接。为了验证节点构造的合理性,揭示其受力工作机理,设计并制作3组节点试件,以轴压比和螺栓等级作为试验变量,进行了3个工况的低周往复拟静力破坏性... 详细信息

提出了一种干式装配式梁柱节点,该节点由核心区梁端外包钢套筒与柱预留孔洞实现螺栓连接。为了验证节点构造的合理性,揭示其受力工作机理,设计并制作3组节点试件,以轴压比和螺栓等级作为试验变量,进行了3个工况的低周往复拟静力破坏性试验。研究节点破坏形式及抗震性能,得到试件的滞回曲线和骨架曲线,分析节点的荷载特征值、延性、耗能和刚度退化。试验结果表明:3个试件的破坏均发生在梁端,节点核心区均没有破坏,满足“强节点弱构件”设计要求;试件滞回曲线饱满,延性系数均大于3.6,黏滞阻尼系数均大于1.5,具有良好的抗震性能;降低螺栓等级能提高节点刚度,但对节点承载力影响有限;增大轴压比时,刚度显著提高,但延性和耗能能力有所降低。在试验结果的基础上,采用ABAQUS对节点进行参数化分析,得出不同参数变化对节点力学性能影响,使试验更有对比性、分析更全面、结论更准确。

关键词：装配式混凝土梁柱节点延性耗能能力抗震性能有限元

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

自杀式恐怖袭击防控探究

辽宁警察学院学报 2024年第4期26卷 33-40页

作者：裴明陈建彤林家熠中国刑事警察学院禁毒与治安学院辽宁沈阳110854 广州市公安局政治部组织处广东广州510000

自杀式恐怖袭击具有恐怖袭击模式与恐怖袭击手段的多重属性,其运行满足以恐怖主体为核心耦合袭击目标、袭击手段、袭击时间和袭击后果等多维恐怖袭击要素的基本存续结构,亦以袭击主体的消亡带动袭击目标受袭形成袭击手段。为有效认知自... 详细信息

自杀式恐怖袭击具有恐怖袭击模式与恐怖袭击手段的多重属性,其运行满足以恐怖主体为核心耦合袭击目标、袭击手段、袭击时间和袭击后果等多维恐怖袭击要素的基本存续结构,亦以袭击主体的消亡带动袭击目标受袭形成袭击手段。为有效认知自杀式恐怖袭击以提升恐怖主义治理效能,从袭击主体、袭击工具和袭击行动的消亡性对其进行剖析,发现基于行为体、承袭体和致损体生成致灾因子、构成致灾机理、形成防控困境,同时也回溯治理逻辑。加强对自杀式恐怖袭击的有效研究,有利于丰富恐怖袭击的研究体系、提升恐怖袭击的应对能力,以期进一步完善恐怖主义的治理工作。

关键词：恐怖主义袭击模式袭击手段自杀式恐怖袭击