Matrix from urine stem cells boosts tissue-specific stem cell mediated functional cartilage reconstruction
作者机构：Stem Cell and Tissue Engineering LaboratoryDepartment of OrthopaedicsWest Virginia UniversityMorgantownWVUSA WVU Cancer InstituteRobert C.Byrd Health Sciences CenterWest Virginia UniversityMorgantownWVUSA Perelman School of MedicineUniversity of PennsylvaniaPhiladelphiaPAUSA Department of BiologyArdabil BranchIslamic Azad UniversityArdabilIran Department of Biochemistry&Molecular GeneticsUniversity of Colorado DenverAuroraCOUSA Department of MicrobiologyImmunologyand Cell BiologyWest Virginia UniversityMorgantownWVUSA Department of Foot and Hand SurgeryClinical Medical College of Yangzhou UniversitySubei People’s Hospital of Jiangsu ProvinceYangzhouJiangsuChina Department of BiostatisticsSchool of Public HealthWest Virginia UniversityMorgantownWVUSA Biomomentum Inc.LavalQuebecCanada Department of BiochemistryWest Virginia UniversityMorgantownWVUSA Bioinformatics CoreWest Virginia UniversityMorgantownWVUSA
出 版 物：《Bioactive Materials》 (生物活性材料（英文）)
年 卷 期：2023年第23卷第5期
页 面：353-367页
核心收录：
学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程]
基 金：supported by grants from the National Institute of Arthritis and Musculoskeletal and Skin Diseases USA
主 题：Decellularized extracellular matrix Tissue-specific stem cell Urine-derived stem cell Rejuvenation Functional cartilage repair
摘 要：Articular cartilage has a limited capacity to self-heal once ***-specific stem cells are a solution for cartilage regeneration;however,ex vivo expansion resulting in cell senescence remains a challenge as a large quantity of high-quality tissue-specific stem cells are needed for cartilage *** previous report demonstrated that decellularized extracellular matrix(dECM)deposited by human synovium-derived stem cells(SDSCs),adipose-derived stem cells(ADSCs),urine-derived stem cells(UDSCs),or dermal fibroblasts(DFs)provided an ex vivo solution to rejuvenate human SDSCs in proliferation and chondrogenic potential,particularly for dECM deposited by *** make the cell-derived dECM(C-dECM)approach applicable clinically,in this study,we evaluated ex vivo rejuvenation of rabbit infrapatellar fat pad-derived stem cells(IPFSCs),an easily accessible alternative for SDSCs,by the abovementioned C-dECMs,in vivo application for functional cartilage repair in a rabbit osteochondral defect model,and potential cellular and molecular mechanisms underlying this *** found that C-dECM rejuvenation promoted rabbit IPFSCs’cartilage engineering and functional regeneration in both ex vivo and in vivo models,particularly for the dECM deposited by UDSCs,which was further confirmed by proteomics ***-Seq analysis indicated that both mesenchymal-epithelial transition(MET)and inflammation-mediated macrophage activation and polarization are potentially involved in the C-dECM-mediated promotion of IPFSCs’chondrogenic capacity,which needs further investigation.
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