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A Uniformly Robust Staggered DG Method for the Unsteady Darcy-Forchheimer-Brinkman Problem

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Communications on Applied Mathematics and Computation 2022年第1期4卷 205-226页

作者： Lina Zhao ming Fai lam Eric Chung Department of Mathematics The Chinese University of Hong KongHong Kong SARChina

In this paper,we propose and analyze a uniformly robust staggered DG method for the unsteady Darcy-Forchheimer-Brinkman *** formulation is based on velocity gradient-velocity-pressure and the resulting scheme can be flexibly applied to fairly general polygonal *** relax the tangential continuity for velocity,which is the key ingredi-ent in achieving the uniform *** present well-posedness and error analysis for both the semi-discrete scheme and the fully discrete scheme,and the theories indicate that the error estimates for velocity are independent of *** numerical experiments are presented to confirm the theoretical findings.

关键词： Staggered DG method Brinkman-Forchheimer General meshes Uniformly stable

Responses in gas exchange and water status between drought-tolerant and-susceptible soybean genotypes with ABA application

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The Crop Journal 2015年第6期3卷 500-506页

作者： Md.Mokter Hossain Hon-ming lam Jianhua Zhang School of Life Sciences and Center for Soybean Research of the State Key Laboratory of Agrobiotechnology The Chinese University of Hong Kong

The purpose of this study was to investigate the physiological responses of drought-tolerant and drought-susceptible soybean genotypes to exogenous abscisic acid(ABA) application during progressive soil drying at seedling stages. Five-day old soybean seedlings were transplanted into PVC tubes filled with soil mixture. Seedlings were watered daily with similar water volumes until second trifoliate leaves emerged, and thereafter soil drying with or without exogenous ABA application was imposed. Half of the seedlings of each genotype were left for regular watering as control plants. Soil water status declined significantly over seven days of withholding water supply for both genotypes. Leaf expansion rate, stomatal conductance(g_s), leaf water potential(ψ_w), and relative water content of leaves(%RWC) declined significantly under soil drying as well as soil drying with ABA application, compared to their values for well-watered soybean genotypes. However, a drought-tolerant genotype(C12) responded more rapidly than a drought-susceptible genotype(C08) after imposition of soil drying and soil drying with exogenous ABA. In addition, application of exogenous ABA to water-restricted soybeans resulted in higher %RWC and ψwin the drought-tolerant than in the drought-susceptible genotype. Compared to the drought-susceptible genotype, the drought-tolerant genotype was more responsive to exogenous ABA application, resulting in a higher root-to-shoot ratio.

关键词： Drought stress Exogenous ABA Leaf relative water content Stomatal conductance Soybean genotypes

Recent Developments of Genomic Research in Soybean

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Journal of Genetics and Genomics 2012年第7期39卷 317-324页

作者： Ching Chan Man-Wah Li Fuk-Ling Wong Hon-ming lam State Key Laboratory of Agrobiolechnology and School of Life Sciences The Chinese University of Hong Kong

Soybean is an important cash crop with unique and important traits such as the high seed protein and oil contents, and the ability to perform symbiotic nitrogen fixation. A reference genome of cultivated soybeans was established in 2010, followed by whole-genome re-sequencing of wild and cultivated soybean accessions. These efforts revealed unique features of the soybean genome and helped to understand its evolution. Mapping of variations between wild and cultivated soybean genomes were perfo^aned. These genomic variations may be related to the process of domestication and human selection. Wild soybean germplasms exhibited hiigh genomic diversity and hence may be an important source of novel genes/alleles. Accumulation of genomic data will help to refine genetic maps and expedite the identification of functional genes. In this review, we summarize the major findings from the whole-genome sequencing projects and discuss the possible impacts on soybean researches and breeding programs. Some emerging areas such as transcriptomic and epigenomic studies will be introduced. In addition, we also tabulated some useful bioinformatics tools that will help the mining of the soybean genomic data.

关键词： Evolution Genome Soybean

Traditional Chinese Medicine diagnoses in persons with ketamine abuse

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Journal of Traditional Chinese Medicine 2013年第2期33卷 164-169页

作者： WaikwongTang ming lam Wingnang Leung Waizhu Sun Tszting Chan Gabor S Ungvari Department of Psychiatry Chinese University of Hong Kong Castle Peak Hospital School of Chinese Medicine Chinese University of Hong Kong Department of Surgery Chinese University of Hong KongHong Kong SARChina School of Psychiatry and Clinical Neurosciences University ofWestern AustraliaPerthAustralia

OBJECTIVES： To explore the distribution of TCM patterns in ketamine users and the inter-rater reli- ability of TCM diagnosis among TCM practitioners. METHODS： Eighty-four subjects recruited from sub- stance abuse clinics and non-governmental coun- seling services catering for ketamine abusers were examined by one or two TCM practitioners. The dis- tribution and inter-rater reliability of the TCM diag- noses between the two TCM practitioners were de- scribed and measured. RESULTS： Seven TCM patterns were differentiated. The four most common patterns were the Heart Yin deficiency （29.8%）, Kidney Yang deficiency （26.2%）, Kidney Yin deficiency （19.0%）, and Spleen Yang defi-ciency （14.3%）. The agreement between the two TCM practitioners was 59%, the kappa for the three most common patterns was 0.472 （P=O.O03）. The proportion of men was lower in the Kidney Yin defi- ciency group （18.8%）, in comparison to the Heart Yin deficiency （60.0%, P=0.009） and Kidney Yang de- ficiency group （59.1%, P=0.013）. CONCLUSION： The differences between the TCM groups in terms of the source of referral and num- ber of drug uses other than ketamine were of bor- derline significance. Further research should focus on validating diagnostic tools and improving in- ter-rater reliability in TCM to enhance the quality of clinical trials of TCM treatments for ketamine abuse.

关键词： Ketamine deficiency SyndromeChinese MedicineAbuse Yin deficiency Yang differentiation Traditional

Putative Nitrogen Sensing Systems in Higher Plants

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Journal of Integrative Plant Biology 2006年第8期48卷 873-888页

作者： Hon-ming lam Ying Ann Chiao Man-Wah Li Yuk-Kwong Yung Sang Ji Department of Biology Chinese University of Hong Kong Shatin Hong Kong Molecular Biotechnology Program Chinese University of Hong Kong Shatin Hong Kong

Nitrogen （N） metabolism is essential for the biosynthesis of vital biomolecules. N status thus exerts profound effects on plant growth and development, and must be closely monitored. In bacteria and fungi, a few sophisticated N sensing systems have been extensively studied. In animals, the ability to receive amino acid signals has evolved to become an integral part of the nervous coordination system. In this review, we will summarize recent developments in the search for putative N sensing systems in higher plants based on homologous systems in bacteria, fungi, and animals. Apparently, although plants have separated and diversified from other organisms during the evolution process, striking similarities can be found in their N sensing systems compared with those of their counterparts; however, our understanding of these systems is still incomplete. Significant modifications of the N sensing systems （including cross-talk with other signal transduction pathways） in higher plants may be a strategy of adaptation to their unique mode of life.

关键词： general amino acid control glutamate receptors His-Asp phosphorelay nitrogen metabolism nitrogen sensing PII, signal transduction.

Inflammatory pseudotumor of the liver in association with a gastrointestinal stromal tumor: A case report

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World Journal of Gastroenterology 2004年第12期10卷 1841-1843页

作者： Oswens S.Lo Ronnie T.Poon Chi ming lam Sheung Tat Fan Centre for the Study of Liver Disease and Department of Surgery The University of Hong KongPokfulamHong KongChina

Inflammatory pseudotumor of the liver is a rare benign lesion that can mimic a malignant liver nioplasm. A case of inflammatory pseudotumor of the liver found in association with a malignant gastrointestinal stromal tumor (GIST) of the small bowel was reported. The inflammatory pseudotumor was misdiagnosed as a metastasis from the GIST by frozen section. A correct diagnosis was made only after histopathological examination of the paraffin section of the resescted specimen. This case is psrticularly interesting because of the association of the two rare pathological entities and the diagnostic dilemma that arose from the similarity of their histological appearances. To our knowledge，this association has not been reported in the literature.

关键词：炎性肿瘤肝脏胃肠基质瘤 GIST 病理组织学

NOD-like receptors mediated activation of eosinophils interacting with bronchial epithelial cells： a link between innate immunity and allergic asthma

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Cellular & Molecular Immunology 2013年第4期10卷 317-329页

作者： Churl Kwok Wong Shuiqing Hu Karen ming-lam Leungt Jie Dong Lan He Yi Jun Chu Ida Miu-Ting Chu Huai-Na Qiu Kelly Yan-Ping Liu ChristopherWai-Kei lam Department of Chemical Pathology The Chinese University of Hong Kong Prince of Wales Hospital Hong Kong China Department of Medicine and Therapeutics The Chinese University of Hong Kong Prince of Wales Hospital Hong Kong China Macao Institute for Applied Research in Medicine and Health Macao University of Science and Technology Taipa Macao China

Key intracytosolic pattern recognition receptors of innate immunity against bacterial infections are nucleotide-binding oligomerization domain （NOD）-Iike receptors （NLRs）. We elucidated the NOD1 and NOD2-mediated activation of human eosinophils, the principal effector cells for allergic inflammation, upon interacting with human bronchial epithelial BEAS-2B cells in allergic asthma. Eosinophils constitutively expressed NOD1,2 but exhibited nonsignificant responses to release chemokines upon the stimulation by NOD1 ligand 7-D-glutamyl-meso-diaminopimelic acid （iE-DAP） and NOD2 ligand muramyl dipeptide （MDP）. However, iE-DAP and MDP could significantly upregulate cell surface expression of CD18 and intercellular adhesion molecule （ICAM）-I on eosinophils and ICAM-1 on BEAS-2B cells, as well as induce chemokines CCL2 and CXCL8 release in the coculture system （all P〈0.05）. Both eosinophils and BEAS-2B cells were the main source for CXCL8 and CCL2 release in the coculture system upon iE-DAP or MDP stimulation. Direct interaction between eosinophils and BEAS-2B cells is responsible for CCL2 release, and soluble mediators are implicated in CXCL8 release. ERK and NF-KB play regulatory roles for the expression of adhesion molecules and chemokines in coculture. Treatment with NOD1,2 ligand could induce the subepithelial fibrosis and significantly enhance the serum concentration of total IgE, chemokine CCL5 for eosinophils and T helper type 2 （Th2） cells and asthma Th2 cytokine IL-13 in bronchoalveolar lavage fluid of ovalbumin-sensitized allergic asthmatic mice （all P〈0.05）. This study provides further evidence of bacterial infection-mediated activation of NOD1,2 in triggering allergic asthma via the activation of eosinophils interacting with bronchial epithelial cells at inflammatory airway.

关键词： allergy bronchial epithelial cells chemokines eosinophils signal transduction

Differences between soybean genotypes in physiological response to sequential soil drying and rewetting

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The Crop Journal 2014年第6期2卷 366-380页

作者： Md Mokter Hossain Xueyi Liu Xusheng Qi Hon-ming lam Jianhua Zhang School of Life Sciences and Center for Soybean Research of the State Key Laboratory of Agrobiotechnology The Chinese University of Hong KongShatin Institute of Economic Crops Shanxi Academy of Agricultural Sciences Institute of Crop Science Gansu Academy of Agricultural Sciences

Soybean genotypes show diverse physiological responses to drought, but specific physiological traits that can be used to evaluate drought tolerance have not been identified. In the present study we investigated physiological traits of soybean genotypes under progressive soil drying and rewetting, using a treatment mimicking field *** a preliminary study with eight soybean genotypes, two drought-tolerant genotypes and one susceptible genotype were grown in the greenhouse and subjected to water restriction. Leaf expansion rate, gas exchange, water relation parameters, total chlorophyll(Chl), proline contents of leaves, and root xylem p H were monitored in a time course, and plant growth and root traits were measured at the end of the stress cycle. Drought-tolerant genotypes maintained higher leaf expansion rate, net photosynthetic rate(Pn), Chl content,instantaneous water use efficiency(WUEi), % relative water content(RWC), water potential(ψw), and turgor potential(ψp) during progressive soil drying and subsequent rewetting than the susceptible genotypes. By contrast, stomatal conductance(gs) and transpiration rate(Tr)of tolerant genotypes declined faster owing to dehydration and recovered more sharply after rehydration than the same parameters in susceptible ones. Water stress caused a significant increase in leaf proline level and root xylem sap p H of both genotypes but tolerant genotypes recovered to pre-stress levels more quickly after rehydration. Tolerant genotypes also produced longer roots with higher dry mass than susceptible genotypes. We conclude that rapid perception and adjustment in response to soil drying and rewetting as well as the maintenance of relatively high Pn, %RWC, and root growth constitute the mechanisms by which drought-tolerant soybean genotypes cope with water stress.

关键词： Drought tolerance Water restriction Photosynthesis Root growth Xylem sap pH Soybean genotypes

Metabolic Regulation and Manipulation of Nitrogen Metabolism: The Case of Asparagine Synthetase

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Metabolic Regulation and Manipulation of Nitrogen Metabolism...