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The terpene synthase(TPS)gene family in kiwifruit shows high functional redundancy and a subset of TPS likely fulfil overlapping functions in fruit flavour,floral bouquet and defence

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Molecular Horticulture 2023年第1期3卷 258-279页

作者： Wu wang mindy Y.wang Yunliu Zeng Xiuyin Chen Xiaoyao wang Anne M.Barrington Jianmin Tao Ross G.Atkinson Niels J.Nieuwenhuizen The New Zealand Institute for Plant and Food Research Ltd(PFR) Private Bag 92169AucklandNew Zealand Institute of Botany Jiangsu Province and Chinese Academy of SciencesNanjing 210014China Key Laboratory of Horticultural Plant Biology College of Horticulture and Forestry ScienceHuazhong Agricultural UniversityWuhan 430070People’s Republic of China College of Horticulture Nanjing Agricultural UniversityNanjing 210095China

Volatile terpenes are important compounds that influence fruit flavour and aroma of *** in plants also impact on the floral bouquet and defence against pests and pathogens in leaves and *** better understand the overlapping roles that terpenes may fulfil in plants,a systematic gene,chemical and biochemical analysis of terpenes and terpene synthases(TPS)was undertaken in Red5 kiwifruit(Actinidia spp.).Analysis of the Red5 genome shows it contains only 22 TPS gene models,of which fifteen encode full-length *** TPS can account for the major terpene volatiles produced in different tissues of Red5 kiwifruit and in response to different *** small Red5 TPS family displays surprisingly high functional redundancy with five TPS producing linalool/*** of leaves and fruit with methyl jasmonate enhanced expression of a subset of defence-related TPS genes and stimulated the release of *** TPS genes were induced upon herbivory of leaves by the economically important insect pest Ctenopseustis obliquana(brown-headed leaf roller)and emission,but not accumulation,of(E)-and(Z)-nerolidol was strongly linked to *** results provide a framework to understand the overlapping biological and ecological roles of terpenes in Actinidia and other horticultural crops.

关键词： Actinidia Aroma Genome analysis Monoterpene Sesquiterpene Volatile

Current update on imaging for pancreatic neuroendocrine neoplasms

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World Journal of Clinical Oncology 2021年第10期12卷 897-911页

作者： Nicole Segaran Catherine Devine mindy.wang Dhakshinamoorthy Ganeshan Department of Radiology Mayo Clinic ArizonaPhoenixAZ 85259United States Department of Diagnostic Radiology The University of Texas MD Anderson Cancer CenterHoustonTX 77030United States Department of Diagnostic Radiology Abdominal ImagingThe University of Texas MD Anderson Cancer CenterHoustonTX 77030United States

Pancreatic neuroendocrine neoplasms(panNEN)are a heterogeneous group of tumors with differing pathological,genetic,and clinical *** on clinical findings,they may be categorized into functioning and nonfunctioning *** of the 2017 World Health Organization classification system,particularly its differentiation between grade 3,well-differentiated pancreatic neuroendocrine tumors(panNET)and grade 3,poorly-differentiated pancreatic neuroendocrine carcinomas(panNEC)has emphasized the role imaging plays in characterizing these *** ultrasound can help obtain biopsy specimen and assess tumor margins and local *** patterns on computed tomography(CT)and magnetic resonance imaging(MRI)may be used to classify *** enhanced MRI and diffusion-weighted imaging have been reported to be useful for characterization of panNEN and quantifying metastatic *** and emerging radiotracers have broadened the utility of functional imaging in evaluating ***-18 fluorodeoxyglucose positron emission tomography(PET)/CT and somatostatin receptor imaging such as Gallium-681,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid–octreotate PET/CT may be useful for improved identification of panNEN in comparison to anatomic *** new techniques can also play a direct role in optimizing the selection of treatment for individuals and predicting tumor response based on somatostatin receptor *** addition,emerging methods of radiomics such as texture analysis may be a potential tool for staging and outcome prediction in panNEN,however further investigation is required before clinical implementation.

关键词： Pancreatic neuroendocrine neoplasms Computed tomography Ultrasound Positron emission tomography Magnetic resonance imaging Peptide receptor radionuclide therapy

Features of primary pancreatic lymphoma:A bi-institutional review with an emphasis on typical and atypical imaging features

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World Journal of Clinical Oncology 2021年第9期12卷 823-832页

作者： Nicole Segaran Kumaresan Sandrasegaran Catherine Devine mindy X wang Chintan Shah Dhakshinamoorthy Ganeshan Department of Radiology Mayo Clinic ArizonaPhoenixAZ 85054United States Department of Diagnostic Imaging University of Texas MD Anderson Cancer CenterHoustonTX 77030United States

BACKGROUND Primary pancreatic lymphoma(PPL)is a rare *** able to distinguish it from other pancreatic malignancies such as pancreatic ductal adenocarcinoma(PDAC)is important for appropriate *** PDAC,PPL is highly sensitive to chemotherapy and usually does not require ***,being able to identify PPL preoperatively will not only direct physicians towards the correct avenue of treatment,it will also avoid unnecessary surgical *** To evaluate the typical and atypical multi-phasic computed tomography(CT)imaging features of *** A retrospective review was conducted of the clinical,radiological,and pathological records of all subjects with pathologically proven PPL who presented to our institutions between January 2000 and December *** review board approval was obtained for this *** collected data were analyzed for subject demographics,clinical presentation,laboratory values,CT imaging features,and the treatment *** of all CT imaging findings including size,site,morphology and imaging characteristics of PPL such as the presence or absence of nodal,vascular and ductal involvement in these subjects were *** those subjects who had a pre-treatment multiphasic CT of the abdomen were included in the *** Twenty-nine cases of PPL were diagnosed between January 2000 and December 2020(mean age 66 years;13 males/16 females).All twenty-nine subjects were symptomatic but only 4 of the 29 subjects(14%)had B *** jaundice occurred in 24%of *** lactate dehydrogenase was seen in 81%of cases,whereas elevated cancer antigen 19-9 levels were present in only 10%of cases for which levels were *** vast majority(90%)of tumors involved the pancreatic head and uncinate *** tumor size was 7.8 cm(range,4.0-13.8 cm).PPL presented homogenous hypoenhancement on CT in 72%of *** volume peripancreatic lymphadenopathy was seen in 28%

关键词： Pancreatic tumors Primary pancreatic lymphoma Pancreatic ductal adenocarcinoma Imaging features Diagnosis Computed tomography

Inactivity of YGL082W in vitro due to impairment of conformational change in the catalytic center loop

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Science China Chemistry 2020年第2期63卷 237-243页

作者： Lining Lu Yu Guo Tian wang Lujun Liang Suwen Zhao Feng wang Lei Liu Tsinghua-Peking Center for Life Sciences Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical Biology(Ministry of Education)Center for Synthetic and Systems BiologyState Key Laboratory of Chemical Oncogenomics(Shenzhen)Department of ChemistryTsinghua UniversityBeijing 100084China School of Life Science Beijing Institute of TechnologyBeijing 100081China iHuman Institute School of Life Science and TechnologyShanghaiTech UniversityShanghai 201210China

mindy-1 is a recently discovered new family of deubiquitinating enzymes(DUB),but one of its yeast homologs,YGL082 W,does not show any DUB activity in *** alignment shows that YGL082 W possesses the correct catalytic triad,and yet did not catalyze either the hydrolysis of di-ubiquitin,crosslinking with C-terminally propargylated ubiquitin,or hydrolysis of *** obtaining a crystal structure of the catalytic domain of YGL082 W,we identified an interesting difference between the catalytic center loop of YGL082 W and that of its human homolog *** the conformation of the catalytic center loop was previously reported to be important for the deubiquitination activity of mindy-1,we hypothesized that Glu27(instead of the corresponding Pro136 in mindy-1) of the catalytic center loop of YGL082 W may impair the conformational change and account for the lack of *** hypothesis was supported by homology modeling and molecular dynamics simulations,which showed that the Pro-to-Glu mutation(P136 E mutation for mindy-1) creates a hydrogen bond that inhibits the conformation change of the catalytic center loop of *** experiments through site-directed mutation validated this hypothesis,showing that the P27 E mutation caused MIY1(a homologous active DUB from yeast) to lose activity.

关键词： deubiquitinating enzymes(DUB) enzymology X-ray crystal structure molecular dynamics

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伦敦制造

艺术与设计 2021年第12期10卷 66-71页

作者：王蛙蛙无不详伦敦博物馆苏格兰国家博物馆莎拉·迈尔斯考夫画廊明迪·所罗门画廊活物画廊

七年前,英国艺术基金(Art Fund)设立了"新收藏奖"(New Collecting Awards),以此为博物馆的艺术品采购项目提供资金支持。最近,作为项目成果展的《伦敦制造》(London Making Now)在伦敦博物馆(Museum of London)展出,展览汇集了15位艺术... 详细信息

七年前,英国艺术基金(Art Fund)设立了"新收藏奖"(New Collecting Awards),以此为博物馆的艺术品采购项目提供资金支持。最近,作为项目成果展的《伦敦制造》(London Making Now)在伦敦博物馆(Museum of London)展出,展览汇集了15位艺术家、设计师、手工艺人的专业作品,生动地展示了伦敦的当代手工艺以及背后丰富的人文世界。

关键词：手工艺人艺术基金人文世界成果展博物馆当代手工艺伦敦

去泛素化酶mindy1是肝癌细胞干性维持因子和肝癌不良预后的独立危险因素

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中华肝脏病杂志 2023年第5期31卷 518-523页

作者：夏搏林刘科伟黄红霞沈美美王斌高建重庆医科大学附属第二医院消化内科重庆400010 陆军军医大学(第三军医大学)大坪医院消化内科消化系统肿瘤精准防治重庆市重点实验室重庆400042 陆军军医大学(第三军医大学)大坪医院重庆400042

目的探索维持肝癌干细胞干性的关键去泛素化酶,为肝癌靶向治疗提供新思路。方法采用CRISPR Screen高通量筛选技术筛选肝癌干细胞干性维持的去泛素化酶。采用RT-qPCR和Western blot分析基因表达水平;使用成球实验、软琼脂克隆形成实验检... 详细信息

目的探索维持肝癌干细胞干性的关键去泛素化酶,为肝癌靶向治疗提供新思路。方法采用CRISPR Screen高通量筛选技术筛选肝癌干细胞干性维持的去泛素化酶。采用RT-qPCR和Western blot分析基因表达水平;使用成球实验、软琼脂克隆形成实验检测肝癌细胞干性;裸鼠皮下荷瘤实验检测肿瘤生长情况;生物信息学及临床样本检测目标基因的临床意义。结果mindy1在肝癌干细胞中高表达,敲除mindy1后干性标志物表达下调、细胞自我更新能力显著降低,且抑制了移植瘤的生长,其作用机制可能与调控Wnt信号通路有关。mindy1在肝癌组织中表达水平高于癌旁,与肿瘤进展密切相关,其高表达是一个肝癌不良预后的独立危险因素。结论去泛素化酶mindy1促进肝癌细胞干性,是一个肝癌不良预后的独立预测因子。

关键词：肝癌干细胞去泛素化酶 mindy1

mindy2过表达慢病毒质粒的构建及其对肺癌A549细胞增殖、迁移和凋亡的影响

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现代肿瘤医学 2023年第7期31卷 1187-1194页

作者：张路瑶李泽艳王晓旋石兴云罗婉媜宋良鹏孙玉玲李得意马雪艳大理大学基础医学院云南大理671000

目的:构建能表达mindy2的慢病毒质粒,并获取稳定过表达mindy2的A549细胞株,探讨过表达mindy2对肺癌A549细胞增殖、迁移和凋亡的影响。方法:采用同源重组法构建pLVX-Flag-mindy2过表达质粒,并通过菌落PCR和测序对重组质粒进行鉴定;在HEK2... 详细信息

目的:构建能表达mindy2的慢病毒质粒,并获取稳定过表达mindy2的A549细胞株,探讨过表达mindy2对肺癌A549细胞增殖、迁移和凋亡的影响。方法:采用同源重组法构建pLVX-Flag-mindy2过表达质粒,并通过菌落PCR和测序对重组质粒进行鉴定;在HEK293T细胞中瞬时转染pLVX-Flag-mindy2质粒,通过Western blot实验检测Flag-mindy2融合蛋白的表达;重组慢病毒载体包装HEK293T细胞,获取病毒上清液感染A549细胞,嘌呤霉素筛选后利用Western blot实验检测Flag-mindy2融合蛋白的表达;CCK8实验、Transwell和划痕实验检测过表达mindy2对A549细胞增殖和迁移能力的影响;流式细胞术检测细胞凋亡及周期变化。结果:测序结果证明成功构建了mindy2过表达慢病毒载体;在HEK293T细胞中瞬时转染mindy2重组质粒,能检测到Flag-mindy2融合蛋白的表达;Western blot结果显示mindy2在A549细胞中稳定过表达;CCK8实验表明过表达mindy2能够抑制A549细胞的增殖;流式细胞术表明过表达mindy2的A549细胞凋亡率显著升高,细胞周期被阻滞在G_(0)/G_(1)期;Transwell和划痕实验表明过表达mindy2能够抑制A549细胞的迁移。结论:成功构建能高效表达mindy2的慢病毒质粒和稳定表达mindy2的A549细胞株;并且mindy2能够抑制肺癌细胞系A549的增殖和迁移,促进细胞的凋亡。

关键词： mindy2 A549细胞增殖凋亡细胞周期迁移