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中华血液学杂志 2003年第5期24卷 272-274页

作者：孟月生 md minden 山东大学第二医院血液肿瘤科济南250033 University Health Network

WTI基因的先天性缺失或功能缺陷可以导致wilms因此普遍被认为是一种抑癌基因，但其在白血病发病中的作用尚有争论~[1，2] 我们应用逆转录病毒载体将WTcDNA导人两种不同的人白血病细胞系K562和U937细胞并获得过度表达，发现Wrl转染白血... 详细信息

WTI基因的先天性缺失或功能缺陷可以导致wilms因此普遍被认为是一种抑癌基因，但其在白血病发病中的作用尚有争论~[1，2] 我们应用逆转录病毒载体将WTcDNA导人两种不同的人白血病细胞系K562和U937细胞并获得过度表达，发现Wrl转染白血病细胞的生长和集落形成能力降低对分化诱导剂的敏感性增加，支持该基因在白血病发病中的抑癌基因说。现将结果报道如下。

关键词：白血病细胞生长细胞集落形成细胞分化 WT1基因

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CLONAL PROLIFERATION AND LONG-TERM CULTURE OF MALIGNANT LYMPHOMA CELLS UNDER SERUM-FREE CULTURE CONDITIONS

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Chinese Journal of Cancer Research 1990年第4期2卷 30-33页

作者：戴育成 Wang XH Wang C Jamal N Biondi A minden md Messner HA Jiangxi Institute of Medical Sciences Nanchang Ontario Cancer Institute TorontoOntario.Canada

We developed a serum-free culture system that promoted the growth of B cell colonies in peripheral blood, bone marrow, lymph nodes and cerebrospinal fluid (CSF) from 7 out of 8 patients with non-Hodgkin's lymphomas of B cell type. The culture cells were pretreated with or without galactose oxi-dase (GO) prior to plating. Colony growth was best supported with BCGF. A moderate increment was observed with rIL-3, as well as rIL-1β and even to a lesser degree, by rlL-2, while B cell stimulating factor-2 (rBCSF-2) and rlL-1β did not show significant activity. rGM-CSF and rG-CSF had little effect, while rM-CSF enhanced the formation of lymphoma colonies. The cells from different patients had different requirements for Staphylococcus aureus protein A and GO pretreatment. It reflected the differences in activation and differentiation status and surface properties of lymphoma cells from different patients. The cells from CSF of one patient were successfully maintained in serum-free culture medium supplemented with 10% BCGF or 5% PHA-LCM for more than 4 months. The long-term culture cells were EBV negative, phenotypically consistent with B cells and gene rearrangements for JH, Kappa and myc. This serum-free culture system allowed extensive analysis of the growth requirements for clonogenic precursors.

关键词： NHL CLONAL PROLIFERATION AND LONG-TERM CULTURE OF MALIGNANT LYMPHOMA CELLS UNDER SERUM-FREE CULTURE CONDITIONS CSF

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The Spatial Structure of Zooplankton Communities of Pedu Reservoir,Malaysia

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湿地科学 2012年第4期10卷 423-428页

作者： Amir Shah Ruddin md Shah Johan Ismail Diana Latief Wan Maznah Wan Omar School of Biological Sciences Universiti Sains MalaysiaMinden11800 PenangMalaysia Department of Animal Science and Fishery Faculty of Agriculture and Food SciencesUniversiti Putra MalaysiaUPM Bintulu Campus97008 BintuluSarawakMalaysia

A total of 22 species of zooplankton were identified from 8 sampling stations located in the limnetic zone of Pedu *** zooplankton community was dominated by rotifers (11 species),followed by cladocerans (9 species) and copepods (2 species).Four species of zooplankton comprised of 3 rotifers,Brachionus quadridentata,Brachionus caudatus and Keratella cochlearis and one cladoceran,Ceriodaphnia cornuta were present at all sampling *** richness was highest at Station 2 (17 species) followed by Station 4 and Station 8 (14 species),Station 5 (15 species),Station 7 (11 species),Station 6 (10 species),Station 3 (9 species) and Station 1 (7 species).With an exception of water transparency,all other water quality parameters such as temperature,dissolved oxygen,conductivity,pH and total dissolved solids were not significant when compared between sampling *** study found that station 5 which was located in a calm strait,protected from strong wave and wind was a suitable area for zooplankton growth and *** supporting the findings included highest water transparency (3.4 m),rich in species numbers (15 species),high diversity index (0.950) and evenness index (0.808).

关键词：水生生物学研究方法演化浮游生物

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Pseudomonas aeruginosa USM-AR2 culture containing biosurfactant facilitates crude oil distillation process

Pseudomonas aeruginosa USM-AR2 culture containing biosurfact...

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2011 International Conference on Biotechnology and Environment Management ( ICBEM 2011）

作者： Nur Asshifa md Noh Salwa Mohd Salleh Mohamad Nasir Mohamad Ibrahim Ahmad Ramli Mohd Yahya School of Biological Sciences Universiti Sains Malaysia 11800 Minden Penang Malaysia School of Chemical Sciences Universiti Sains Malaysia 11800 Minden Penang Malaysia

Petroleum supplies a large portion of the world’s energy, thereby driving industrial civilization. It remains an important factor in dictating market prices of diverse products and services. Distillation of crude oil is a common fractionation method of petroleum producing desirable products, such as automotive fuel and kerosene. Conventional distillation has been the current fractionation process for decades, producing distillates of interest and is conducted without any involvement of biological materials. The process involves tremendous amount of thermal energy and requiring a long time. This research offers an improved distillation performance and yield. A small amount of rhamnolipid produced from a microbial culture Pseudomonas aeruginosa USM-AR2, is added and mixed thoroughly with the crude oil prior to distillation. This results in a drastic reduction in distillation time without affecting or reducing the quality of the distillate. The pretreatment accelerates the emulsification and biodegradation process by decreasing surface tension and viscosity of oil, which in turn resulted in the shorter distillation times. Reducing the distillation times translates to energy and cost savings in producing petroleum products. Since oil prices affect many other industries, this discovery will have a great impact on the economy.

关键词： rhamnolipid distillation crude oil emulsification Pseudomonas aeruginosa

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Effect of Rice AUXIN BINDING PROTEIN57(OsABP57)Overexpression in Response to Flooding

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Journal of Agricultural Science and Technology(B) 2020年第3期10卷 125-133页

作者： Zarith Ayunie Zambrose Sitti Aisyah Mohd Roszelin Nur Aminah Binti Mohd Hazbir Bee Lynn Chew Siti Sarah Jumali Wan Asrina Wan Yahya Nurulhikma md Isa Department of Biological Sciences and Biotechnology Faculty of Science and TechnologyUniversiti Kebangsaan MalaysiaUKM Bangi 43600SelangorMalaysia School of Biological Sciences Universiti Sains MalaysiaMinden 11800GeorgetownPenangMalaysia Faculty of Plantation and Agrotechnology UiTM Melaka BranchMerlimau 77300MelakaMalaysia Department of Crop Science Faculty of Agriculture and Food SciencesUniversiti Putra MalaysiaSarawak CampusBintulu 97008Sarawak

This study reports the effects of Oryza sativa AUXIN BINDING PROTEIN57(OsABP57)overexpression towards flooding in ***57 was previously reported to activate plasma membrane H+-*** studies address the ability of transgenic OsABP57 overexpression in enduring drought and salinity stresses but none on the *** this study,complete submergence analysis was carried out and several morphophysiological parameters were analyzed such as plant height,root architecture and relative chlorophyll *** showed that there are no differences between OsABP57 overexpression rice compared to MR219 control rice in terms of chlorophyll content and plant height after 1-3 weeks of flooding *** analysis,however,found that transgenic rice OsABP57 produced more adventitious roots compared to MR219 rice under normal condition,which may be due to the role of the gene that encodes for auxin binding *** semi-quantitative polymerase chain reaction(PCR)on Oryza sativa pyruvate decarboxylase(OsPDC)gene after two weeks of flooding treatment showed an increase of expression in OsABP57 transgenic compared to ***,the overexpression of OsABP57 did not show any significant difference in terms of morphophysiological analysis between the transgenic line and MR219,yet,there is an increase of OsPDC gene in the transgenic background which may need further experimental analysis in the future to map the network between auxin and hypoxia core genes.

关键词： Oryza sativa flooding OsABP57 MR219 hypoxia.

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Power Conversion Efficiency of Schottky Diode Fabricated on AIGaAs/GaAs Heterostructure

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材料科学与工程（中英文版） 2010年第6期4卷 60-65页

作者： Parimon Norfarariyanti Mustafa Farahiyah Hashim Abdui Manaf Abd Rahman Shaharin Fadzli Abdul Rahman Abdul Rahim Osman Mohd Nizam Abd Aziz Azlan Hashim md. Roslan Material Innovations and Nanoelectronics Research Group Faculty of Electrical Engineering Universiti Teknologi MalaysiaSkudai 81310 Johor Malaysia Telekom Research ＆ Development TM Innovation Centre Cyberjaya 63000 Malaysia Nano-Optoelectronics Research Faculty of Physics Universiti Sains Malaysia Minden 11800 Penang Malaysia

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