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Journal of Integrative Plant Biology 2016年第11期58卷 874-889页

作者： Hyunsook Kim Mikiko Kojima Daeseok Choi Soyoung Park Minami Matsui Hitoshi Sakakibara Ildoo Hwang Department of Life Sciences Pohang University of Science and Technology Pohang 790-784 Korea Synthetic Genomics Research Team Biomass Research Cooperation Division (BMEP) RIKEN Center for Sustainable Resource Science (CSRS) Tsurumi Yokohama 230-0045 Japan Riken Center for Sustainable Resource Science (CSRS) Tsurumi Yokohama 230-0045 Japan

Cambial activity is a prerequisite for secondary growth in plants; however, regulatory factors controlling the activity of the secondary meristem in radial growth remain elusive. Here, we identified INCREASED CAMBIAL ACTIVITY （ICA）, a gene encoding a putative pectin methyltransferase, which could function as a modulator for the meristematic activity of fascicular and interfascicular cambium in Arabidopsis. An overexpressing transgenic line, 35S：＇1CA, showed accelerated stern elongation and radial thickening, resulting in increased accumulation of biomass, and increased levels of cytokinins （CKs） and gibberellins （GAs）. Expression of genes encoding pectin methylesterases involved in pectin modification together with pectin methyltransferases was highly induced in 355：：ICA, which might contribute to an increase of methanol emission as a byproduct in 35S：ICA. Methanol treatment induced the expression of GA- or CK-responsive genes and stimulated plant growth. Overall, we propose that ectopic expression of ICA increases cambial activity by regulating CK and GA homeostasis, and methanol emission, eventually leading to stem elongation and radial growth in the inflorescence stem.

关键词： Cambial activity cytokinin gibberellin methanol methyltransferase pectin shoot growth

Intraarterial and intravenous contrast enhanced CT and MR imaging of multi-step hepatocarcinogenesis defining the early stage of hepatocellular carcinoma development

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Hepatoma Research 2020年第6期6卷 107-120页

作者： Satoshi Kobayashi Kazuto Kozaka Toshifumi Gabata Osamu matsui Tetsuya minami Department of Quantum Medical Technology Kanazawa University Graduate School of Medical SciencesKanazawa 920-0942Japan Department of Radiology Kanazawa University Graduate School of Medical SciencesKanazawa 920-8641Japan Department of Radiology Kanazawa Medical UniversityUchinada 920-0293Japan

Liver cancer is the second leading cause of cancer deaths in men worldwide,and the 6th and 7th cause of cancer deaths in men and women in developed countries.70%-90%of primary liver cancer is hepatocellular carcinoma.Hepatitis B or C viruses and chronic inflammation due to alcohol intake are the main risk factors for hepatocellular carcinoma.One of the key approaches for the early detection of hepatocellular carcinoma is to understand the specific imaging findings of liver nodules in the multi-step hepatocrcinogenesis process.In this article,we review the imaging findings of multistep hepatocarcinogenesis,with a focus on the early detection of malignant,cirrhotic nodules with CT and MRI.

关键词： Hepatocellular carcinoma multistep hepatocarcinogenesis early hepatocellular carcinoma dysplastic nodule CT MRI

SD3, an Arabidopsis thaliana Homolog of TIM21, Affects Intracellular ATP Levels and Seedling Development

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Molecular Plant 2012年第2期5卷 461-471页

作者： Hidefumi Hamasaki Takeshi Yoshizumi Naoki Takahashi Mieko Higuchi Takashi Kuromori Yuko Imura Hiroaki Shimada Minami Matsui Plant Functional Genomics Research Group Plant Science Center RIKEN Yokohama Institute 1-7-22 Suehiro-cho Tsurumi-ku Yokohama Kanagawa 230-0045 Japan Department of Biological Science and Technology Tokyo University of Science 2641 Yamazaki Noda Chiba 278-8510 Japan Gene Discovery Research Group RIKEN Plant Science Center RIKEN Yokohama Institute 1-7-22 Suehiro-cho Tsurumi-ku Yokohama Kanagawa 230-0045 Japan

It is poorly understood how plants control their growth by cell division, elongation, and differentiation. We have characterized a seedling-lethal mutant segregation distortion 3 （sd3） that showed a very dwarf phenotype when grown in the light and, in the dark, had short hypocotyls with reduced ploidy levels. The corresponding gene of SD3 encodes a protein with high similarity to yeast translocase on the inner mitochondrial membrane 21 （TIM21）, which is a component of the TIM23 complex. Indeed, SD3 protein fused to GFP localized in the mitochondria. SD3 overexpression increased cotyledon size in the light and hypocotyl thickness in the dark. The expression of genes for several subunits of the respiratory-chain complexes III and IV was up-regulated in SD3-overexpressing plants. Furthermore, these plants showed high levels of ATP whereas those of sd3 were low. These results suggested that SD3 induced an increase in cell size by raising the expression of the respiratory-chain subunit genes and hence increased the intracellular ATP levels, We propose that intracellular ATP levels regulated by mitochondria control plant organ size.

关键词： hypocotyl elongation endoreduplication mitochondria ATR

Metabolomic Screening Applied to Rice FOX Arabidopsis Lines Leads to the Identification of a Gene-Changing Nitrogen Metabolism

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Molecular Plant 2010年第1期3卷 125-142页

作者： Doris Albinsky Miyako Kusano Mieko Higuchi Naomi Hayashi Makoto Kobayashi Atsushi Fukushima Masaki Mori Takanari Ichikawa Keiko matsui Hirofumi Kuroda Yoko Horii Yuko Tsumoto Hitoshi Sakakibara Hirohiko Hirochika Minami Matsui Kazuki Saito RIKEN Plant Science Center Tsurumi-ku Yokohama 230-0045 Japan National Institute of Agrobiological Sciences Tsukuba 305-8602 Japan Graduate School of Pharmaceutical Sciences Chiba University Inage-ku Chiba 263-8522 Japan

Plant metabolomics developed as a powerful tool to examine gene functions and to gain deeper insight into the physiology of the plant cell. In this study, we screened Arabidopsis lines overexpressing rice full-length （FL） cDNAs （rice FOX Arabidopsis lines） using a gas chromatography-time-of-flight mass spectrometry （GC-TOF/MS）-based technique to identify rice genes that caused metabolic changes. This screening system allows fast and reliable identification of candi- date lines showing altered metabolite profiles. We performed metabolomic and transcriptomic analysis of a rice FOX Ara- bidopsis line that harbored the FL cDNA of the rice ortholog of the Lateral Organ Boundaries （LOB） Domain （LBD）/ Asymmetric Leaves2-1ike （ASL） gene of Arabidopsis, At-LBD37/ASL39. The investigated rice FOX Arabidopsis line showed prominent changes in the levels of metabolites related to nitrogen metabolism. The transcriptomic data as well as the results from the metabolite analysis of the Arabidopsis At-LBD37/ASL39-overexpressor plants were consistent with these findings. Furthermore, the metabolomic and transcriptomic analysis of the Os-LBD37/ASL39-overexpressing rice plants indicated that Os-LBD37/ASL39 is associated with processes related to nitrogen metabolism in rice. Thus, the combination of a metabolomics-based screening method and a gain-of-function approach is useful for rapid characterization of novel genes in both Arabidopsis and rice.

关键词： Metabolite profiling GC-TOF/MS rice FOX Arabidopsis lines transcript profiling LBD37/ASL39 nitrogenmetabolism.

Novel Approaches for Inhibitor Screening and Target Identification using Arabidopsis Full-Length cDNA Overexpression （FOX） Lines

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Novel Approaches for Inhibitor Screening and Target Identifi...

A High-Throughput Screening System for Arabidopsis Transcription Factors and Its Application to Med25-Dependent Transcriptional Regulation

The 1st International Conference on Plant Secondary Metabolism

作者： Hiroyuki Kasahara Takanari Ichikawa Minami Matsui Yuji Kamiya RIKEN Plant Science CenterYokohama Japan RIKEN Plant Science Center Yokohama Japan

＜正＞Organisms have two possible routes for the biosynthesis of isoprenoids;the mevalonate （MVA） and methylerythritol phosphate （MEP） pathways.The MEP pathway has been recently recognized as important target of drug and agrochemical developments and chemical genetics studies,e.***.fosmidomycin shows antima- larial activity by inhibition of 1-deoxyxylulose-5-phosphate reductoisomerase （DXR）,and clomazone has been used for herbicide,which metabolite ketoclomazone inhibits 1-deoxyxylulose-5-phosphate synthase.Plants have

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Molecular Plant 2011年第3期4卷 546-555页

作者： Bin Oua Kang-Quan Yin Sai-Nan Liu Yan Yang Tren Gu Jennifer Man Wing Hui Li Zhang Jin Miao Youichi Kondou Minami Matsui Hong-Ya Gu Li-Jia Qu National Laboratory for Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing 100871 People's Republic ofChina Plant Science Center RIKEN Yokohama Institute 1-7-22 Suehiro-cho Tsurumi-ku Yokohama City Kanagawa 230-0045 Japan The National Plant Gene Research Center (Beijing) Beijing 100101 People's Republic of China

The activities of transcription factors （TFs） require interactions with specific DNA sequences and other reg- ulatory proteins. To detect such interactions in Arabidopsis, we developed a high-throughput screening system with a Gateway-compatible Gal4-AD-TF library of 1589 Arabidopsis TFs, which can be easily screened by mating-based yeast-one-hybrid （YIH） and yeast-two-hybrid （Y2H） methods. The efficiency of the system was validated by examining two well-characterized TF-DNA and TF-protein interactions： the CHE-CCA1 promoter interaction by YIH and NPR1-TGAs interactions by Y2H. We used this system to identify eight TFs that interact with a Mediator subunit, Med25, a key reg- ulator in JA signaling. We identified five TFs that interacted with the GCC-box cis-element in the promoter of PDF1.2, a downstream gene of Med25. We found that three of these TFs, all from the AP2-EREBP family, interact directly both with Med25 and the GCC-box of PDF1.2, suggesting that Med25 regulates PDF1.2 expression through these three TFs. These results demonstrate that this high-throughput Y1H/Y2H screening system is an efficient tool for studying transcrip- tional regulation networks in Arabidopsis. This system will be available for other Arabidopsis researchers, and thus it provides a vital resource for the Arabidopsis community.

关键词： Transcription factors Arabidopsis yeast-one-hybrid yeast-two-hybrid Med25.

The Arabidopsis COP9 SIGNALOSOME INTERACTING F-BOX KELCH 1 Protein Forms an SCF Ubiquitin Ligase and Regulates Hypocotyl Elongation

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Molecular Plant 2013年第5期6卷 1616-1629页

作者： Anna Franciosini Benedetta Lombardi Silvia lafrate Valeria.Pecce Giovanni Mele Leonardo Lupacchini Gianmarco Rinaldi Youichi Kondou Giuliana Gusmaroli Shiori Aki Tomohiko Tsuge Xing-Wang Deng Minami Matsui Paola Vittorioso Paolo Costantin Giovanna Serino Dipartimento di Biologia e Biotecnologie Sapienza Universita di Roma Italy Istituto di Biologia e Biotecnologia Agraria Consiglio Nazionale delle Ricerche Italy RIKEN Plant Science Center Yokohama Japan Department of Molecular Cellular and Development Biology Yale University New Haven CT USA Present address: Department of Biology University of South Carolina Beaufort Bluff'ton AC USA Molecular Biology Laboratory Institute for Chemical Research Kyoto University Japan The New York Botanical Garden Bronx NY USA

The regulation of protein turnover by the ubiquitin proteasome system （UPS） is a major posttranslational mechanism in eukaryotes. One of the key components of the UPS, the COP9 signalosome （CSN）, regulates ＇cullin-ring＇ E3 ubiquitin ligases. In plants, CSN participates in diverse cellular and developmental processes, ranging from light signaling to cell cycle control. In this work, we isolated a new plant-specific CSN-interacting F-box protein, which we denominated CFK1 （COP9 INTERACTING F-BOX KELCH 1）. We show that, in Arabidopsis thaliana, CFK1 is a component of a functional ubiquitin ligase complex. We also show that CFK1 stability is regulated by CSN and by proteasome-dependent proteoly- sis, and that light induces accumulation of the CFK1 transcript in the hypocotyl. Analysis of CFK1 knockdown, mutant, and overexpressing seedlings indicates that CFK1 promotes hypocotyl elongation by increasing cell size. Reduction of CSN levels enhances the short hypocotyl phenotype of CFKl-depleted seedlings, while complete loss of CSN activity sup- presses the Iong-hypocotyl phenotype of CFKl-overexpressing seedlings. We propose that CFK1 （and its regulation by CSN） is a novel component of the cellular mechanisms controlling hypocotyl elongation.

关键词： Arabidopsis thaliana ubiquitin COP9 signalosome proteasome F-box protein hypocotyl elongation seed-ling development.

丙型肝炎性肝硬化者肝中静脉引流区选择性萎缩:采用多层CT的形态测量研究

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国际医学放射学杂志 2011年第1期34卷 82-页

作者： K.Ozaki O.matsui S.Kobayashi J.Sanada W.Koda T.minami 王化 Department of Radiology Graduate School of Medical ScienceKanazawa University

目的采用多层CT容积测量法回顾性分析丙型肝炎相关肝硬化的形态学改变,其通常表现为大结节性硬化,与门静脉血供及肝静脉引流相关。

关键词：肝硬化形态学改变静脉引流肝中静脉丙型肝炎病毒性肝炎 CT