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Neuroprotective effects of electroacupuncture on early- and late-stage spinal cord injury

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Neural Regeneration Research 2015年第10期10卷 1628-1634页

作者： min-fei wu Shu-quan Zhang Jia-bei Liu Ye Li Qing-san Zhu Rui Gu Department of Orthopedics Bethune Second Hospital of Jilin University Department of Orthopedics Tianjin Nankai Hospital Department of Orthopedics China-Japan Union Hospital Jilin University

Previous studies have shown that the neurite growth inhibitor Nogo-A can cause secondary neural damage by activating Rho A. In the present study, we hypothesized that electroacupuncture promotes neurological functional recovery after spinal cord injury by inhibiting Rho A expression. We established a rat model of acute spinal cord injury using a modification of Allen＇s method. The rats were given electroacupuncture treatment at Dazhui（Du14）, mingmen（Du4）, Sanyinjiao（SP6）, Huantiao（GB30）, Zusanli（ST36） and Kunlun（BL60） acupoints with a sparsedense wave at a frequency of 4 Hz for 30 minutes, once a day, for a total of 7 days. Seven days after injury, the Basso, Beattie and Bresnahan（BBB） locomotor scale and inclined plane test scores were significantly increased, the number of apoptotic cells in the spinal cord tissue was significantly reduced, and Rho A and Nogo-A m RNA and protein expression levels were decreased in rats given electroacupuncture compared with rats not given electroacupuncture. Four weeks after injury, pathological tissue damage in the spinal cord at the site of injury was alleviated, the numbers of glial fibrillary acidic protein- and neurofilament 200-positive fibers were increased, the latencies of somatosensory-evoked and motor-evoked potentials were shortened, and their amplitudes were increased in rats given electroacupuncture. These findings suggest that electroacupuncture treatment reduces neuronal apoptosis and decreases Rho A and Nogo-A m RNA and protein expression at the site of spinal cord injury, thereby promoting tissue repair and neurological functional recovery.

关键词： nerve regeneration spinal cord injury electroacupuncture locomotion Rho A Nogo-A glial fibrillary acidic protein neurofilament 200 neural regeneration

Transplantation of human telomerase reverse transcriptase gene-transfected Schwann cells for repairing spinal cord injury

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Neural Regeneration Research 2015年第12期10卷 2040-2047页

作者： Shu-quan Zhang min-fei wu Jia-bei Liu Ye Li Qing-san Zhu Rui Gu Department of Orthopedics Tianjin Nankai Hospital Department of Spine Surgery Orthopedic HospitalSecond HospitalClinical HospitalJilin University Department of Orthopedics China-Japan Union HospitalJilin University

Transfection of the human telomerase reverse transcriptase（h TERT）gene has been shown to increase cell proliferation and enhance tissue *** the present study,h TERT was transfected into rat Schwann cells.A rat model of acute spinal cord injury was established by the modified free-falling *** PLXSN was injected at the site of spinal cord injury as a vector to mediate h TERT gene-transfected Schwann cells（1×10^（10）/L;10μL）or Schwann cells（1×10^（10）/L;10μL）without h TERT gene *** 1 and 4 weeks after model establishment,motor function of the lower limb improved in the h TERT-transfected group compared with the group with non-transfected Schwann *** deoxynucleotidyl transferase-mediated d UTP nick-end labeling and reverse transcription-polymerase chain reaction results revealed that the number of apoptotic cells,and gene expression of aquaporin 4/9 and matrix metalloproteinase 9/2decreased at the site of injury in both groups;however,the effect improved in the h TERT-transfected group compared with the Schwann cells without h TERT transfection *** and eosin staining,PKH26 fluorescent labeling,and electrophysiological testing demonstrated that compared with the non-transfected group,spinal cord cavity and motor and sensory evoked potential latencies were reduced,while the number of PKH26-positive cells and the motor and sensory evoked potential amplitude increased at the site of injury in the h TERT-transfected *** findings suggest that transplantation of h TERT gene-transfected Schwann cells repairs the structure and function of the injured spinal cord.

关键词： nerve regeneration spinal cord injury Schwann cells transplantation motor function telomerase reverse transcriptase proliferation modification cells neural regeneration

Transplantation of erythropoietin gene-modified neural stem cells improves the repair of injured spinal cord

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Neural Regeneration Research 2015年第9期10卷 1483-1490页

作者： min-fei wu Shu-quan Zhang Rui Gu Jia-bei Liu Ye Li Qing-san Zhu Department of Orthopedics the Second Hospital of Jilin University Department of Orthopedics Tianjin Nankai Hospital Department of Orthopedics China-Japan Union Hospital of Jilin University

The protective effects of erythropoietin on spinal Here, the eukaryotic expression plasmid pcDNA3.1 cord injury have not been well described. human erythropoietin was transfected into rat neural stem cells cultured in vitro. A rat model of spinal cord injury was established using a free falling object. In the human erythropoietin-neural stem cells group, transfected neural stem cells were injected into the rat subarachnoid cavity, while the neural stem cells group was inject- ed with non-transfected neural stem cells. Dulbecco＇s modified Eagle＇s medium/F12 medium was injected into the rats in the spinal cord injury group as a control. At 1-4 weeks post injury, the motor function in the rat lower limbs was best in the human erythropoietin-neural stem ceils group, followed by the neural stem cells group, and lastly the spinal cord injury group. At 72 hours, compared with the spinal cord injury group, the apoptotic index and Caspase-3 gene and protein expressions were apparently decreased, and the bd-2 gene and protein expressions were noticeably increased, in the tissues surrounding the injured region in the human erythro- poietin-neural stem cells group. At 4 weeks, the somatosensory evoked potential latencies were cavities were clearly smaller and the motor and remarkably shorter in the human erythropoi- etin-neural stem cells group and neural stem cells group than those in the spinal cord injury group. These differences were particularly obvious in the human erythropoietin-neural stem cells group. More CM-Dil-positive cells and horseradish peroxidase-positive nerve fibers and larger amplitude motor and somatosensory evoked potentials were found in the human erythro- poietin-neural stem cells group and neural stem cells group than in the spinal cord injury group. Again, these differences were particularly obvious in the human erythropoietin-neural stem cells group. These data indicate that transplantation of erythropoietin gene-modified neural stem cells into the su

关键词： nerve regeneration spinal cord injury neural stem cells erythropoietin motor function subarachnoid cavity transplantation injury recovery neural regeneration

Edaravone combined with Schwann cell transplantation may repair spinal cord injury in rats

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Neural Regeneration Research 2015年第2期10卷 230-236页

作者： Shu-quan Zhang min-fei wu Zhe Piao Jin Yao Ji-hai Li Xin-gang Wang Jun Liu Department of Orthopedics Tianjin Nankai Hospital Department of Spine Surgery Orthopedic Hospital Second Hospital Clinical Hospital Jilin University Department of Burns and Plastic Surgery China-Japan Union Hospital Jilin University Department of Hand and Foot Surgery and Reparative & Reconstructive Surgery Center Orthopedic Hospital Second Hospital Clinical Hospital Jilin University

Edaravone has been shown to delay neuronal apoptosis, thereby improving nerve function and the microenvironment after spinal cord injury. Edaravone can provide a favorable environment for theAa：eatment of spinal cord injury using Schwann cell transplantation. This study used rat models of complete spinal cord transection at T9. Six hours later, Schwann cells were transplanted in the head and tail ends of the injury site. Simultaneously, edaravone was injected through the caudal vein. Eight weeks later, the PKH-26-1abeled Schwann cells had survived and migrated to the center of the spinal cord injury region in rats after combined treatment with edaravone and Schwann cells. Moreover, the number of PKH-26-1abeled Schwann cells in the rat spinal cord was more than that in rats undergoing Schwann cell transplantation alone or rats without any treatment. Horseradish peroxidase retrograde tracing revealed that the number of horserad- ish peroxidase-positive nerve fibers was greater in rats treated with edaravone combined with Schwann cells than in rats with Schwann cell transplantation alone. The results demonstrated that lower extremity motor function and neurophysiological function were better in rats treated with edaravone and Schwann cells than in rats with Schwann cell transplantation only. These data confirmed that Schwann cell transplantation combined with edaravone injection promoted the regeneration of nerve fibers of rats with spinal cord injury and improved neurological function.

关键词： nerve regeneration spinal cord injury Schwann cells cell transplantation edaravone motor function electrophysiological function neural regeneration

Senegenin inhibits neuronal apoptosis after spinal cord contusion injury

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Neural Regeneration Research 2016年第4期11卷 657-663页

作者： Shu-quan Zhang min-fei wu Rui Gu Jia-bei Liu Ye Li Qing-san Zhu Jin-lan Jiang Department of Orthopedics Nankai Hospital Department of Orthopedics the Second Hospital of Jilin University Department of Orthopedics China-Japan Union HospitalJilin University Scientific Research Center China-Japan Union HospitalJilin University

Senegenin has been shown to inhibit neuronal apoptosis,thereby exerting a neuroprotective *** the present study,we established a rat model of spinal cord contusion injury using the modified Allen＇s *** hours after injury,senegenin（30 mg/g） was injected into the tail vein for 3 consecutive *** reduced the size of syringomyelic cavities,and it substantially reduced the number of apoptotic cells in the spinal *** the site of injury,Bax and Caspase-3 m RNA and protein levels were decreased by senegenin,while Bcl-2 m RNA and protein levels were *** fiber density was increased in the spinal cord proximal to the brain,and hindlimb motor function and electrophysiological properties of rat hindlimb were *** together,our results suggest that senegenin exerts a neuroprotective effect by suppressing neuronal apoptosis at the site of spinal cord injury.

关键词： nerve regeneration spinal cord contusion senegenin thinleaf milkwort root motor function apoptosis electrophysiology neural regeneration

Co-production of hydrogen, oxygen, and electricity via an integrated solar-driven system with decoupled water electrolyzer and Na-Zn ion battery

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Journal of Energy Chemistry 2025年第1期100卷 621-627页

作者： fei Lv Longjie Liu Jiazhe wu Pengfei Wang Lixia Pan Dengwei Jing Yubin Chen International Research Center for Renewable Energy State Key Laboratory of Multiphase Flow in Power Engineering Xi'an Jiaotong University

Combining water electrolysis and rechargeable battery technologies into a single system holds great promise for the co-production of hydrogen （H2） and ***,the design and development of such systems is still in its ***,an integrated hydrogen-oxygen （O2）-electricity co-production system featuring a bipolar membrane-assisted decoupled electrolyzer and a Na-Zn ion battery was established with sodium nickelhexacyanoferrate （NaNiHCF） and Zn2+/Zn as dual redox *** decoupled electrolyzer enables to produce H2and O2in different time and space with almost 100%Faradaic efficiency at 100 mA ***,the charged NaNiHCF and Zn electrodes after the electrolysis processes formed a Na-Zn ion battery,which can generate electricity with an average cell voltage of 1.75 V at 10 m A *** connecting Si photovoltaics with the modular electrochemical device,a well-matched solar driven system was built to convert the intermittent solar energy into hydrogen and electric energy with a solar to hydrogen-electricity efficiency of 16.7%,demonstrating the flexible storage and conversion of renewables.

关键词： Hydrogen Electricity Decoupled water electrolyzer Na-Zn ion battery Solar energy

Porous Organic Cage‑Based Quasi‑Solid‑State Electrolyte with Cavity‑Induced Anion‑Trapping Effect for Long‑Life Lithium Metal Batteries

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Nano-Micro Letters 2025年第2期17卷 376-386页

作者： Wei-min Qin Zhongliang Li Wen‑Xia Su Jia‑min Hu Hanqin Zou Zhixuan wu Zhiqin Ruan Yue‑Peng Cai Kang Li Qifeng Zheng School of Chemistry South China Normal UniversityGuangzhou 510006People’s Republic of China Key Laboratory of Functional Metal‑Organic Compounds of Hunan Province College of Chemistry and Material ScienceHengyang Normal UniversityHengyang 421008People’s Republic of China Guangzhou Key Laboratory of Energy Conversion and Energy Storage Materials Guangzhou 510006People’s Republic of China

Porous organic cages(POCs)with permanent porosity and excellent host–guest property hold great potentials in regulating ion transport behavior,yet their feasibility as solid-state electrolytes has never been testified in a practical ***,we design and fabricate a quasi-solid-state electrolyte(QSSE)based on a POC to enable the stable operation of Li-metal batteries(LMBs).Benefiting from the ordered channels and cavity-induced anion-trapping effect of POC,the resulting POC-based QSSE exhibits a high Li+transference number of 0.67 and a high ionic conductivity of 1.25×10^(−4) S cm^(−1) with a low activation energy of 0.17 *** allow for homogeneous Li deposition and highly reversible Li plating/stripping for over 2000 *** a proof of concept,the LMB assembled with POC-based QSSE demonstrates extremely stable cycling performance with 85%capacity retention after 1000 ***,our work demonstrates the practical applicability of POC as SSEs for LMBs and could be extended to other energy-storage systems,such as Na and K batteries.

关键词： Porous organic cage Cavity-induced anion-trapping Quasi-solid-state electrolyte Homogeneous Li+flux Lithium metal battery

改性硅藻土对重金属离子吸附性能工艺条件的优化

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广州化工 2018年第15期46卷 81-83,99页

作者：滕敏徐立飞吴红六盘水师范学院化学与材料工程学院贵州六盘水553004

采用XRD、XRF、SEM等分析手段探讨物理、化学改性前后硅藻土的理化性质,研究了其对重金属离子Cu2+、Pb2+的吸附性能,并对工艺条件进行优化。结果表明,硅藻土经过改性处理后,富集了Si元素的含量,杂质的含量降低,孔道结构更清晰。当吸附... 详细信息

采用XRD、XRF、SEM等分析手段探讨物理、化学改性前后硅藻土的理化性质,研究了其对重金属离子Cu2+、Pb2+的吸附性能,并对工艺条件进行优化。结果表明,硅藻土经过改性处理后,富集了Si元素的含量,杂质的含量降低,孔道结构更清晰。当吸附时间为30 min,初始p H值为7的工艺条件下,物理改性硅藻土对Cu2+的去除率达到87.56%;当初始p H值为7,吸附时间为60 min的条件下化学改性硅藻土对Pb2+的去除率为88%。

关键词：硅藻土改性重金属离子吸附性能工艺条件

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银行规模、金融生态环境与小企业融资

科学决策 2017年第9期 25-43页

作者：李华民吴非廉胜南韩亚欣广东金融学院广东区域金融政策研究中心华南师范大学经济与管理学院广州区域金融政策重点研究基地

时下主流观点强调解决小企业融资难题在于发挥"小银行优势"。然而基于此理论的政策导向与实践,并未有效疏通小企业融资瓶颈。本文以广州、中山、惠州和江门等广东省4个地市调研数据为样本,将金融生态环境要素纳入了不同规模的银行机构... 详细信息

时下主流观点强调解决小企业融资难题在于发挥"小银行优势"。然而基于此理论的政策导向与实践,并未有效疏通小企业融资瓶颈。本文以广州、中山、惠州和江门等广东省4个地市调研数据为样本,将金融生态环境要素纳入了不同规模的银行机构面向小企业融资的分析框架之中,探讨不同金融生态环境下,面向小企业融资的大小银行机构的行为差异,实证求解为小企业融资的主力金融机构格局。结果表明,一方面,小企业同大银行机构建立金融关系,更能优化自身的融资约束困境;另一方面,合意的金融生态环境,有助于大银行机构提高小企业的融资境遇。本文的政策含义在于,化解小企业融资难题的最有效金融结构,重在改善金融生态环境,并由此激发大银行机构充分发挥其功能。

关键词：小企业融资银行结构金融生态环境