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Journal of Environmental Sciences 2011年第3期23卷 353-359页

作者： min gao linli junxin liu Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China

We investigated the correlation between performance and the bacteria community composition by H2S and toluene co-treatment. Operation of the bioreactor was divided into four stages, in which the inlet concentration of toluene and H2S were gradually increased. In Stage I, toluene was the sole target compound with an average removal efficiency of 86.49%. After adding HeS in Stage II, removal efficiency of toluene decreased immediately and recovered gradually to 85.96%. When the inlet concentration of toluene and HzS was increased in Stage III and Stage IV, respectively, the average removal efficiency for toluene increased continuously from 86.31% to 87.24%. The elimination capacities of toluene increased with increasing inlet loading rates of toluene and H2S. Results of the PCR-DGGE analysis showed a turnover growth and decline of the microbial populations in the bioreactor. In Stage I, the dominant toluene-degrading bacteria mainly contained Pseudomonas sp. strain PS ＋ and Hydrogenophaga sp. In Stage IV, however, the dominant toluene-degrading bacteria was aciduric bacteria （Clostridium populeti）. The dominant microbial community in the bioreactor enhanced the elimination capacity of toluene, and adding H2S changed the environment of microbial growth, thus resulted in an evolution of dominant microorganisms. Analyses of microbial community and their activities provides valuable information to efficiently enhance simultaneous removal of toluene and H2S in the bioreactor.

关键词： off-gas treatment bacterial community hydrogen sulfate toluene

Removal of airborne microorganisms emitted from a wastewater treatment oxidation ditch by adsorption on activated carbon

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Journal of Environmental Sciences 2011年第5期23卷 711-717页

作者： Lin Li min gao junxin liu Xuesong Guo Department of Water Pollution Control Technology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China

Bioaerosol emissions from wastewater and wastewater treatment processes are a significant subgroup of atmospheric aerosols. Most previous work has focused on the evaluation of their biological risks. In this study, however, the adsorption method was applied to reduce airborne microorganisms generated from a pilot scale wastewater treatment facility with oxidation ditch. Results showed adsorption on granule activated carbon （GAC） was an efficient method for the purification of airborne microorganisms. The GAC itself had a maximum adsorption capacity of 2217 CFU/g for airborne bacteria and 225 CFU/g for fungi with a flow rate of 1.50 m^3/hr. Over 85% of airborne bacteria and fungi emitted from the oxidation ditch were adsorbed within 80 hr of continuous operation mode. Most of them had a particle size of 0.65-4.7 μm. Those airborne microorganisms with small particle size were apt to be adsorbed. The SEM/EDAX, BET and Boehm＇s titration methods were applied to analyse the physicochemical characteristics of the GAC. Relationships between GAC surface characteristics and its adsorption performance demonstrated that porous structure, large surface area, and hydrophobicity rendered GAC an effective absorber of airborne microorganisms. Two regenerate methods, ultraviolet irradiation and high pressure vapor, were compared for the regeneration of used activated carbon. High pressure vapor was an effective technique as it totally destroyed the microorganisms adhered to the activated carbon. Microscopic observation was also carried out to investigate original and used adsorbents.

关键词： activated carbon adsorption airborne microorganisms oxidation ditch wastewater treatment facility

A novel integrated step-feed biofilm process for the treatment of decentralized domestic wastewater in rural areas of China

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Journal of Environmental Sciences 2010年第3期22卷 321-327页

作者： Hanwen Liang, min gao, junxin liu, Yuansong Wei, Xuesong Guo Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. [a] Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China

For wastewater treatment in rural areas, a novel three-stage step-feed wastewater treatment system, combined with a drop-aeration biofilm process, was tested in the laboratory to investigate its performance in removing suspended solids （SS）, chemical oxygen demand （COD）, NH4＋-N, total nitrogen （TN）, and total phosphorus （TP）. The removal rates of SS, COD and NH4＋-N were 90%, 80%, and 90% in efluent concentrations less than 10 mg/L, 50 mg/L and 8 mg/L, respectively. The TP removal rate was less satisfactory. The C/N ratio in the raw wastewater was often less than 3.5, and the removal efficiency of TN was therefore limited. A carbon-release batch experiment was carried out to measure the feasibility of enhancing denitrification at low influent C/N ratios. The result showed that the C/N could be over 9.0 in the supernatant. Polymerase chain reaction denaturing gradient gel electrophoresis technology was used to reveal the changes in the bacterial community during different stages of the integrated step-feed biofilm process. The results showed that banding patterns and the distribution of dominant bands for the same experimental period in different aerobic zones were similar. Phylogenetic analysis indicated that lanes 10, 11 and 12, which presented three aerobic zones at the same operation period, had the closest phylogenetic relationship among the lanes.

关键词： step-feed biofilm removal efficiency PCR-DGGE C/N ratio

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TA1/2A12复合板成形性能的模拟研究

锻压技术 2025年第1期50卷 74-82页

作者：高利涛刘鑫鑫卫俊鑫曹守臻冯浩贾燚韩建超太原理工大学机械工程学院太原理工大学先进金属复合材料成形技术与装备教育部工程研究中心海安太原理工大学先进制造与智能装备产业研究院

采用Dynaform有限元软件对不同放置顺序的TA1/2A12复合板进行胀形试验模拟，分析了TA1/2A12复合板极限胀形高度、各层板材的应变和减薄率分布情况，并进行胀形试验验证。模拟结果表明，当钛层与铝层分别放置在靠近凸模的位置时，极限胀... 详细信息

采用Dynaform有限元软件对不同放置顺序的TA1/2A12复合板进行胀形试验模拟，分析了TA1/2A12复合板极限胀形高度、各层板材的应变和减薄率分布情况，并进行胀形试验验证。模拟结果表明，当钛层与铝层分别放置在靠近凸模的位置时，极限胀形高度分别为33.9和32.0 mm。钛层半球底部附近的应力较高，沿TD方向应变较大；铝层应力较小且分布均匀，因此钛层位于半球底部附近的位置先达到极限应变，在复合板结合作用下，铝层相应位置的应变随之增加直至破裂。由胀形试验可得，钛层、铝层分别靠近凸模的位置时，极限胀形高度分别为35.7和33.6 mm,与有限元模拟的极限胀形高度的误差分别为5.3%和5.0%,模拟与实际破裂情况比较吻合，所建立的有限元模型可以较为准确地预测钛/铝复合板的成形性能。

关键词：钛/铝复合板胀形极限胀形高度应力减薄率

Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment

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aBIOTECH 2023年第3期4卷 202-212页

作者： linli Huang Guangqi gao Congcong Jiang Guimei Guo Qiang He Yingjie Zong Chenghong liu Ping Yang Biotech Research Institute Shanghai Academy of Agricultural Sciences/Shanghai Key Laboratory of Agricultural Genetics and BreedingShanghai 201106China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China

Induced mutations are important for genetic research and *** induced by physical or chemical mutagenesis are usually heterozygous during the early ***,mutations must be fixed prior to phenotyping or field trials,which requires additional rounds of *** culture is an effective method to produce double-haploid(DH)plants that are fixed *** this study,we conducted ethyl methanesulfonate(EMS)-induced mutagenesis of microspore cultures of barley(Hordeum vulgare)cultivar‘Hua30’and landrace‘HTX’.The EMS concentrations were negatively correlated with the efficiency of callus induction and the frequency of mutant plant *** two genotypes showed different regeneration *** phenotypic variation of the regenerated M1 plants and the presence of genome-wide nucleotide mutations,revealed by whole-genome sequencing,highlight the utility of EMS-induced mutagenesis of isolated microspore cultures for developing DH ***-wide analysis of the mutation frequency in the regenerated plants revealed that a considerable proportion of mutations resulted from microspore culture(somaclonal variation)rather than EMS-induced *** addition to producing a population of 1972 homozygous mutant lines that are available for future field trials,this study lays the foundation for optimizing the regeneration efficiency of DH plants and the richness of mutations(mainly by fine-tuning the mutagen dosage).

关键词： Barley Mutagenesis Microspore culture Double-haploid(DH) Homozygous mutant

Phytohormones Accumulation and Distribution in Shoots and Roots of Haploid,Diploid and Tetraploid Barley Seedlings Derived from Microspore Culture

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Phyton-International Journal of Experimental Botany 2022年第7期91卷 1419-1428页

作者： Longhua Zhou Ting He Jing Li Guimei Guo Yingbo Li Hongwei Xu Runhong gao linli Huang Yifei Wang Ruiju Lu Zhiwei Chen Chenghong liu Biotech Research Institute Shanghai Academy of Agricultural SciencesShanghai200000China Biotechnology Research Institute Shanghai Key Laboratory of Agricultural Genetics and BreedingShanghai200000China Shanghai Baixin Biotechnology Company Limited Shanghai200000China

Phytohormones play important roles in plant growth and development,and polyploids are thought to be an important method for plant ***,the relationship between ploidy and phytohormone is still *** this study,barley at three ploidy levels were produced by microspore ***,we further analyzed the phytohormone content in the shoots and roots of the three kinds of barley materials to study the effect of ploidy on phytohormones accumulation and *** results showed that Abscisic acid(ABA),gibberellin(GA),jasmonic acid(JA),auxin(IAA),salicylic acid(SA)and cytokinin(CTK)were successfully determined in shoots and roots using LC-MS(liquid chromatography mass spectrometry).By comparing the shoots of the haploid and diploid plants,it was found that the distribution trend of the six phytohormones was consistent,and another consistent trend was found in the roots of the diploid and tetraploid *** addition,we further analyzed the shoot/root ratio of the different phytohormones to identify the potential differences for haploid,diploid and ***,the relationship between ploidy and phytohormone we provided would provide new insights into understanding the new phenotypes that occur in polyploid species.

关键词： Barley polyploidy microspore culture phytohormone shoot root

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典型城市污水处理工艺微生物气溶胶逸散研究

给水排水 2010年第9期36卷 146-150页

作者：高敏李琳刘俊新中国科学院生态环境研究中心

污水处理过程中产生微生物气溶胶，其对人体健康和环境的影响与其组成、浓度和粒径有关。采用6级撞击式空气微生物采样器分别对采用氧化沟和A2／O工艺的两座城市污水处理厂的细菌和真菌气溶胶浓度与粒径进行检测分析，结果显示，空间相... 详细信息

污水处理过程中产生微生物气溶胶，其对人体健康和环境的影响与其组成、浓度和粒径有关。采用6级撞击式空气微生物采样器分别对采用氧化沟和A2／O工艺的两座城市污水处理厂的细菌和真菌气溶胶浓度与粒径进行检测分析，结果显示，空间相对封闭的污泥脱水间内细菌和真菌气溶胶浓度最高；曝气方式对细菌气溶胶的产生量影响较大，而对真菌气溶胶产生量的影响较小；两座污水处理厂内最高浓度的细菌气溶胶和真菌气溶胶的粒径都出现在2．1～3．3μm，但粒径分布的范围受曝气方式的影响而略有差异。为污水处理工艺设计时控制微生物气溶胶逸散方法的选择提供了科学依据。

关键词：污水处理微生物气溶胶真菌细菌粒径特征

基于NSGA-Ⅲ的白车身焊装生产平台的离散拓扑优化

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上海交通大学学报 2020年第12期54卷 1324-1334页

作者：高云凯马超刘哲田林雳同济大学汽车学院上海201804 武汉理工大学现代汽车零部件技术湖北省重点实验室武汉430070 武汉理工大学汽车零部件技术湖北省协同创新中心武汉430070

针对第3代非支配排序遗传算法(NSGA-Ⅲ)求解离散拓扑优化收敛性差的问题,提出一种改进的NSGA-Ⅲ(mNSGA-Ⅲ),用于某白车身焊装生产平台的结构优化.提出新型极值点选择算法,以稳定种群中个体的归一化过程.建立该生产平台的有限元模型,并... 详细信息

针对第3代非支配排序遗传算法(NSGA-Ⅲ)求解离散拓扑优化收敛性差的问题,提出一种改进的NSGA-Ⅲ(mNSGA-Ⅲ),用于某白车身焊装生产平台的结构优化.提出新型极值点选择算法,以稳定种群中个体的归一化过程.建立该生产平台的有限元模型,并以其总质量、最大应力和多个节点的z向位移最小为目标,进行离散拓扑优化.利用MATLAB集成***软件,二次开发离散拓扑优化程序.通过优化、筛选,获得合理的杆件布置设计方案,使得结构总质量降低了30.1%,且刚度和强度均符合标准.优化结果表明,mNSGA-Ⅲ在求解高维多目标离散拓扑优化方面,具有计算过程稳定、收敛速度较快等优点,为大型钢结构的优化设计提供了新方法,具备工程实际应用价值.

关键词：离散拓扑优化多目标优化非支配排序遗传算法

加强医疗质量与患者安全的团队策略和工具在护理安全教育中应用的范围综述

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中华护理教育 2023年第8期20卷 925-931页

作者：谢林莉敬洁高义刘丹菊四川省医学科学院·四川省人民医院成都市610031

目的对加强医疗质量与患者安全的团队策略和工具(Team Strategies and Tools to Enhance Performance and Patient Safety,TeamSTEPPS)在护理安全教育中的应用研究进行范围审查,为未来TeamSTEPPS在护理安全教育中的应用提供参考。方法以... 详细信息

目的对加强医疗质量与患者安全的团队策略和工具(Team Strategies and Tools to Enhance Performance and Patient Safety,TeamSTEPPS)在护理安全教育中的应用研究进行范围审查,为未来TeamSTEPPS在护理安全教育中的应用提供参考。方法以Arksey和O’Malley的范围综述方法为框架,检索万方、中国知网、维普、中国生物医学文献数据库、PubMed、CINAHL、Web of Science、Scopus和Cochrane Library数据库中TeamSTEPPS在护理安全教育中应用的文献,检索时限为建库至2023年4月18日,按照纳入和排除标准筛选文献,对所纳入文献的信息进行汇总分析。结果共检索出858篇文献,最终纳入18篇文献;TeamSTEPPS的教育对象以临床护士为主(n=12),教学实施形式主要包括工作坊、角色扮演、情景演练、团建活动等,TeamSTEPPS在护理安全教育中应用的局限性包括样本量小、应用时长较短、缺乏统一可靠的教学效果评价工具等。结论TeamSTEPPS在护理安全教育中的教学实施形式灵活,能够满足受训者个性化教学需求,提升其安全知识和安全技能。未来需要进一步拓展教育对象和教学场景,应用患者安全结局相关评价指标,全方位评估TeamSTEPPS在护理安全教育中的应用效果。

关键词：护理学患者安全综述文献(主题) 加强医疗质量与患者安全的团队策略和工具

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造林对土壤碳储量影响的研究

生态学杂志 2005年第4期24卷 410-416页

作者：史军刘纪远高志强崔林丽中国科学院地理科学与资源研究所北京100101 中国科学院遥感应用研究所北京100101

全球气候变化和土地利用变化,体现了自然和人类活动对陆地生态系统的影响。造林,作为一种土地利用变化,能够改变陆地土壤碳储量。但其作用结果具有不确定性,受到多种因素的制约。本文对造林后土壤碳储量变化和影响这种变化的因素及其影... 详细信息

全球气候变化和土地利用变化,体现了自然和人类活动对陆地生态系统的影响。造林,作为一种土地利用变化,能够改变陆地土壤碳储量。但其作用结果具有不确定性,受到多种因素的制约。本文对造林后土壤碳储量变化和影响这种变化的因素及其影响效果进行综述,介绍了土壤碳储量变化的研究方法,最后指出当前研究的不足和今后的努力方向。随着我国造林面积的扩大和人工林年龄的增加,加强造林后土壤碳积累机制及这种积累的空间分异和时间动态的研究。

关键词：造林土壤碳储量人工林影响因素研究方法