Simultaneous removal of hydrogen sulfide and toluene in a bioreactor:Performance and characteristics of microbial community

作     者：Min Gao LinLi Junxin Liu 

作者机构：Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2011年第23卷第3期

页      面：353-359页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 09[农学] 0903[农学-农业资源与环境] 090301[农学-土壤学] 

基　　金：supported by the National Natural Science Foundation of China (No. 50921064) 

主　　题：off-gas treatment bacterial community hydrogen sulfate toluene 

摘      要：We investigated the correlation between performance and the bacteria community composition by H2S and toluene co-treatment. Operation of the bioreactor was divided into four stages, in which the inlet concentration of toluene and H2S were gradually increased. In Stage I, toluene was the sole target compound with an average removal efficiency of 86.49%. After adding HeS in Stage II, removal efficiency of toluene decreased immediately and recovered gradually to 85.96%. When the inlet concentration of toluene and HzS was increased in Stage III and Stage IV, respectively, the average removal efficiency for toluene increased continuously from 86.31% to 87.24%. The elimination capacities of toluene increased with increasing inlet loading rates of toluene and H2S. Results of the PCR-DGGE analysis showed a turnover growth and decline of the microbial populations in the bioreactor. In Stage I, the dominant toluene-degrading bacteria mainly contained Pseudomonas sp. strain PS ＋ and Hydrogenophaga sp. In Stage IV, however, the dominant toluene-degrading bacteria was aciduric bacteria （Clostridium populeti）. The dominant microbial community in the bioreactor enhanced the elimination capacity of toluene, and adding H2S changed the environment of microbial growth, thus resulted in an evolution of dominant microorganisms. Analyses of microbial community and their activities provides valuable information to efficiently enhance simultaneous removal of toluene and H2S in the bioreactor.