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Broad-Spectrum Blast Resistance： Harnessing a Natural Allele of a Transcription Factor in Rice

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Molecular Plant 2017年第9期10卷 1144-1146页

作者： Hiroshi Takatsuji Institute of Agrobiological Sciences (NIAS) National Agriculture and Food Research Organization (NARO) 2-1-2 Kannondai Tsukuba Ibaraki 305-0051 Japan

Rice blast, which is caused by the fungus Magnaporthe oryzae, is a widespread and destructive plant disease that greatly reduces yield and grain quality worldwide. The use of host resistance is the most effective and economic way to control plant diseases. Many studies on blast resistance genes have focused on the nucleotide-binding site leucine-rich repeat （NB-LRR） resistance （called typical R） genes that mediate effector-triggered immunity. However, incorporating these R genes into rice varieties has not achieved durable blast resistance, because R-gene-mediated resistance is race-specific and often breaks down owing to the appearance of fungal variants that escape host recognition. Thus, the deployment of genes conferring non-race-specific du- rable resistance is highly desired by breeders. In plants, several genes conferring non-race-specific durable resistance genes have been isolated, but only a few of the molecular mechanisms responsible for resistance have been determined.

关键词： Broad-Spectrum Blast Resistance Harnessing a Natural Allele Transcription Factor in Rice

Near-infrared imaging survey of faint companions around young dwarfs in the Pleiades cluster

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Research in Astronomy and Astrophysics 2011年第3期11卷 335-344页

作者： Yoichi Itoh Yumiko Oasa Hitoshi Funayama Masahiko hayashi Misato Fukagawa Toshio Hashiguchi Thayne Currie Graduate School of Science Kobe University1-1 RokkodaiNada-kuKobe 657-8501Japan Faculty of Education Saitama University255 Shimo-OkuboSakura-kuSaitamaSaitama 338-8570Japan Subaru Telescope 650 North A'ohoku PlaceHiloHawaii 96720USA School of Mathematical and Physical Science Graduate University for Advanced Studies(Sokendai)2-21-1 OsawaMitakaTokyo 181-8588Japan Graduate School of Science Osaka UniversityMachikaneyamaToyonakaOsaka 560-0043Japan NASA-Goddard Space Flight Center

We conducted a near-infrared imaging survey of 11 young dwarfs in the Pleiades cluster using the Subaru Telescope and the near-infrared coronagraph imager. We found ten faint point sources,with magnitudes as faint as 20 mag in the K-band,with around seven *** with the Spitzer archive images revealed that a pair of the faint sources around V 1171 Tau is very red in infrared wavelengths,which indicates very low-mass young stellar ***,the results of our follow-up proper motion measurements implied that the central star and the faint sources do not share common proper motions,suggesting that they are not physically associated.

关键词： planetary systems:formation—techniques:high angular resolution

Effects of gamma-ray irradiation on electronic and non-electronic equipment of Large Helical Device

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Plasma Science and Technology 2017年第2期19卷 95-100页

作者： K OGAWA T NISHITANI M ISOBE M SATO M YOKOTA H hayashi T KOBUCHI T NISHIMURA National Institute for Fusion Science National Institutes of Natural Sciences322-6 0roshi-choToki 509-5292Japan SOKENDAI(The Graduate University for Advanced Studies) School of Physical Sciences322-6 Oroshi-choTold 509-5292Japan

In a deuterium operation on the Large Helical Device, the measurement and control equipment placed in the toms hall must survive under an environment of radiation, To study the effects of gamma-ray irradiation on the equipment, an irradiation experiment is performed at the Cobalt-60 irradiation facility of Nagoya University. Transient and permanent effects on a personal computer, media converters, programmable logic controllers, isolation amplifiers, a web camera, optical flow meters, and water sealing gaskets are experimentally surveyed. Transient noise appears on the web camera. Offset of the signal increases with an increase of the integrated dose on the programmable logic controller. The DeviceNet module on the programmable logic controller is broken at the integrated dose of 72 Gy, which is the expected range of the integrated dose of the toms hall. The other equipment can survive under the gamma-ray field in the toms hall.

关键词： large helical device gamma-ray irradiation radiation effect

Characteristics of gas evolution profiles during coal pyrolysis and its relation with the variation of functional groups

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International Journal of Coal Science & Technology 2018年第4期5卷 452-463页

作者： Lu Zhang Shichao Qi Norihiro Takeda Shinji Kudo Junichiro hayashi Koyo Norinaga Department of Applied Science for Electronics and Materials Kyushu University6-1Kasuga KoenKasugaFukuoka 816-8580Japan Institute for Materials Chemistry and Engineering Kyushu University6-1Kasuga KoenKasugaFukuoka 816-8580Japan

Strict relation between the substituents or functional groups attached to the coal macromolecules and the generation of the volatile products, e.g., CH4 H2O, CO, CO2, etc., during the coal pyrolysis is an important but confusing subject. In this paper, quadrupole mass spectronletry, gas chromatography, and ^l3C nuclear magnetic resonance are applied to real-time monitoring the formations of volatile products, off-line quantitative determination of the total products from the pyrolysis of a sub-bituminous coal (SC), and the changes of diverse substitents in the SC along with coke foamation, respectively. These measurements are also performed for the pyrolysis of a caking coal to contrast SC. The qualitative and quantitative data reveal that, during coal pyrolysis, the functional groups related with the formation of CO, i.e., ether, carbonyl, and anhydride, can directly gen erate CO via bond breaking, or take a detour of the formation of other intermediates via condensation and recombination firstly. Moreover, the formations of CO2 and CH4 are related to the direct removal of -COO- and -CH3,respectively.

关键词： Light gas evolution Carbonization QMS GC ^13C NMR

Acceleration of Cell Growth by Xyloglucan Oligosaccharides in Suspension-Cultured Tobacco Cells

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Molecular Plant 2010年第3期3卷 549-554页

作者： Rumi Kaid Satoko Sugawara Kanako Negoro Hisae Maki Takahisa hayashi Takako S. Kaneko Department of Chemical and Biological Sciences Japan Women's University Tokyo 112-8681 Japan Research Institute for Sustainable Humanosphere Kyoto University Kyoto 611-0011 Japan Institute of Sustainability Science Kyoto University Kyoto 611-0011 Japan

The incorporation of xyloglucan oligosaccharide （XXXG） into the walls of suspension-cultured tobacco cells accelerated cell expansion followed by cell division, changed cell shape from cylindrical to spherical, decreased cell size, and caused cell aggregation. Fluorescent XXXG added to the culture medium was found to be incorporated into the surface of the entire wall, where strong incorporation occurred not only on the surface, but also in the interface walls between cells during cell division. Cell expansion was always greater in the transverse direction than in the longitudinal direction and then, immediately, expansion led to cell division in the presence of XXXG; this process might result in the high level of cell aggregation seen in cultured tobacco cells. We concluded that the integration of this oligosaccharide into the walls could accelerate not only cell expansion, but also cell division in cultured cells.

关键词： Xyloglucan oligosaccharide ceil expansion cell division wall loosening suspension-cultured cells.

A Combined Computational and Experimental Study on the Structure-Regulation Relationships of Putative Mammalian DNA Replication Initiator GINS

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Genomics, Proteomics & Bioinformatics 2006年第3期4卷 156-164页

作者： Reiko hayashi Takako Arauchi Moe Tategu Yuya Goto Kenichi Yoshida Department of Life Sciences Meiji University School of Agriculture Kawasaki Kanagawa 214-8571 Japan.

GINS, a heterotetramer of SLD5, PSF1, PSF2, and PSF3 proteins, is an emerging chromatin factor recognized to be involved in the initiation and elongation step of DNA replication. Although the yeast and Xenopus GINS genes are well documented, their orthologous genes in higher eukaryotes are not fully characterized. In this study, we report the genomic structure and transcriptional regulation of mammalian GINS genes. Serum stimulation increased the GINS mRNA levels in human cells. Reporter gene assay using putative GINS promoter sequences revealed that the expression of mammalian GINS is regulated by 17β-Estradiolstimulated estrogen receptor a, and human PSF3 acts as a gene responsive to transcription factor E2F1. The goal of this study is to present the current data so as to encourage further work in the field of GINS gene regulation and functions in mammalian cells.

关键词： DNA replication initiation bioinformatics gene structure gene regulation 17β-Estradiol transcription factor E2F1

Genetic polymorphisms of MAFK, encoding a small Maf protein, are associated with susceptibility to ulcerative colitis in Japan

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World Journal of Gastroenterology 2017年第29期23卷 5364-5370页

作者： Tomiyasu Arisawa Masakatsu Nakamura Toshimi Otsuka Wu Jing Naoko Sakurai Hikaru Takano Tasuku hayashi Masafumi Ota Tomoe Nomura Ranji hayashi Takeo Shimasaki Tomomitsu Tahara Tomoyuki Shibata Department of Gastroenterology Kanazawa Medical University Department of Gastroenterology Fujita Health University

AIM To investigate whether single nucleotide polymorphisms in maf protein K(MAFK), which encodes the MAFK, lead to increased susceptibility to ulcerative colitis in the Japanese population. METHODS This case control study examined the associations between MAFK single nucleotide polymorphisms(rs4268033 G>A, rs3735656 T>C and rs10226620 C>T) and ulcerative colitis susceptibility in 174 patients with ulcerative colitis(UC) cases, and 748 subjects without no lower abdominal symptoms, diarrhea or hematochezia(controls). In addition, as the second controls, we set 360 subjects, who have an irregular bowel movement without abnormal lower endoscopic findings(IBM controls).RESULTS The genotype frequency of rs4268033 AA and allelic frequency of the rs4268033 A allele were significantly higher in the UC cases than in both controls(P = 0.0005 and < 0.0001, P = 0.015 and 0.0027 vs controls and IBM controls, respectively). Logistic regression analysis after adjustment for age and gender showed that the rs4268033 AA and rs3735656 CC genotypes were significantly associated with susceptibility to UC development(OR = 2.63, 95%CI: 1.61-4.30, P = 0.0001 and OR = 1.81; 95%CI: 1.12-2.94, P = 0.015, respectively). Similar findings were observed by the comparison with IBM controls. In addition, the rs4268033 AA genotype was significantly associated with all phenotypes of UC except early onset. There was no significant association between rs10226620 and ulcerative colitis. CONCLUSION Our results provide the first evidence that MAFK genetic polymorphisms are significantly associated with susceptibility to UC development. In particular, rs4268033 is closely associated with an increased risk for the development of UC.

关键词： Maf 蛋白质 K 基因多型性反应的氧种类 Ulcerative 大肠炎原子 factor-erythroid 2-related 因素 2

Genetic data indicate that most field-collected woodroach pairs are unrelated

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Insect Science 2017年第3期24卷 522-526页

作者： Hajime Yaguchi Yoshinobu hayashi Takafumi Tohoku Christine A. Nalepa Kiyoto Maekawa Graduate School of Science and Engineering University of Toyama Toyama Japan Graduate School of Environmental Science Hokkaido University Sapporo Japan Department of Entomology North Carolina State University Raleigh North Carolina USA

Dear Editor, Woodroaches in the genus Cryptocercus are subsocial cockroaches that utilize decaying logs for both shelter and as a food source （Nalepa, 1984）. Based on molecular phylogenetic studies of multiple independent data （e.g., Lo et al., 2000; Inward et al., 2007）, Cryptocercus and termites are shown to have a sister group relationship within the cockroach clade. The 2 groups also share mul- tiple traits related to ecology, social structure, symbionts, and digestive physiology. Consequently, to discuss euso- cial e-~olution in termites, Cryptocercus would serve as an important group for a ＂model-taxon by homology＂（Klass et al., 2008）.

关键词： CRYPTOCERCUS-PUNCTULATUS DICTYOPTERA WOOD-FEEDING COCKROACHES SCUDDER DICTYOPTERA TERMITES

Nanodot array deposition via single shot laser interference pattern using laser-induced forward transfer

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International Journal of Extreme Manufacturing 2020年第2期2卷 114-118页

作者： Yoshiki Nakata Eiki hayashi Koji Tsubakimoto Noriaki Miyanaga Aiko Narazaki Tatsuya Shoji Yasuyuki Tsuboi Institute of Laser Engineering Osaka University2-6 YamadaokaSuitaOsaka 565-0871Japan Institute for Laser Technology 1-8-4 Utsubo-honmachiNishi-kuOsaka 550-0004Japan National Institute of Advanced Industrial Science and Technology Chuo 21-1-1 UmezonoTsukubaIbaraki 305-8568Japan Department of Chemistry Osaka City University3-3-138SugimotoSumiyoshi-kuOsaka 558-8585Japan

Laser-induced forward transfer(LIFT)is a direct-writing technique capable of depositing a single dot smaller than the laser wavelength at small shot energy through the laser-induced dot transfer(LIDT)*** deposit a single nanodot in a single shot of laser irradiation,a liquid nanodrop is transferred from donor to receiver and finally solidified via a solid–liquid–solid(SLS)*** conventional LIDT experiments,multi-shots with step scanning have been used to form array ***,interference laser processing can achieve an arrayed process and generate a periodic structure in a single *** this study,a femtosecond laser interference pattern was first applied to LIDT,and an array of nanodots was successfully deposited in a single shot,producing the following unit structures:a single dot,adjoining dots,and stacking *** diameter of the smallest nanodot was 355 nm,and the narrowest gap between two adjoining nanodots was 17.2 *** LIDT technique produces high-purity,catalyst-free that do not require post-cleaning or alignment *** these significant advantages,LIDT can expand the usability of nanodots in a wide range of fields.

关键词： interference laser processing laser-induced dot transfer nanodot array femtosecond laser solid-liquid-solid mechanism Au