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Gene-modified leucoconcentrate for personalized ex vivo gene therapy in a mini pig model of moderate spinal cord injury

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Neural Regeneration Research 2021年第2期16卷 357-361页

作者： Rustem R.Islamov Farid V.Bashirov mikhail e.sokolov Andrei A.Izmailov Filip O.Fadeev Vage A.Markosyan Maria A.Davleeva Olga V.Zubkova Maxim M.Smarov Denis Yu.Logunov Boris S.Naroditskyi Ilnur I.Salafutdinov Albert A.Rizvanov Ramil G.Turaev Kazan State Medical University KazanRussia Gamaleya Research Institute of epidemiology and Microbiology MoscowRussia Kazan Federal University KazanRussia The Republican Blood Center of the Ministry of Health of the Republic of Tatarstan KazanRussia

We previously demonstrated that gene-modified umbilical cord blood mononuclear cells overexpressing a combination of recombinant neurotrophic factors are a promising therapeutic approach for cell-mediated gene therapy for neurodegenerative diseases,neurotrauma,and *** this study,using a mini pig model of spinal cord injury,we proposed for the first time the use of gene-modified leucoconcentrate prepared from peripheral blood in the plastic blood bag for personalized ex vivo gene *** obtained from mini pig peripheral blood was transduced with a chimeric adenoviral vector(Ad5/35 F)that carried an enhanced green fluorescent protein(eGFP)reporter gene in the plastic blood *** day after blood donation,the mini pigs were subjected to moderate SCI and four hours post-surgery they were intravenously autoinfused with gene-modified leucoconcentrate.A week after gene-modified leucoconcentrate therapy,fluorescent microscopy revealed eGFP-expressing leucocytes in spinal cord at the site of contusion *** the spleen the groups of eGFP-positive cells located in the lymphoid follicles were *** vitro flow cytometry and fluorescent microscopy studies of the gene-modified leucoconcentrate samples also confirmed the production of eGFP by ***,the efficacy of leucocytes transduction in the plastic blood bag and their migratory potential suggest their use for temporary production of recombinant biologically active molecules to correct certain pathological *** paper presents a proof-of-concept of simple,safe and effective approach for personalized ex vivo gene therapy based on gene-modified leucoconcentrate *** animal protocols were approved by the Kazan State Medical University Animal Care and Use Committee(approval No.5)on May 27,2014.

关键词： chimeric Ad5/35F virus enhanced green fluorescent protein gene-modified leucoconcentrate mini pig peripheral blood personalized ex vivo gene therapy plastic blood bag spinal cord injury

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Burns and frostbite in the Red Army during World War Ⅱ

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Military Medical Research 2017年第3期4卷 121-126页

作者： Vladimir sokolov Alexey Biryukov Igor Chmyrev mikhail Tarasenko Pavel Kabanov Department of Thermal Injuries of the S.M. Kirov Military Medical Academy

The start of World War Ⅱ(WWⅡ) led to the deployment of combat troops in several continents. Destruction and many casualties among both the military and civilians became an inevitable consequence. A large amount of people injured were in need of life-saving treatment and a speedy return to duty. Intensive studies of the specific issues of diagnosis and treatment of thermal injury were conducted in the Soviet Union before the war. The first special units for patients with burn injuries were created, and the first specialists received their first clinical experience. The contributions of famous Soviet scientists in the development of the treatment of burns and frostbite in WWⅡ are studied in this article. The structure of thermal injuries among military personnel and the results of their treatment are shown. Treatment, classification and quantity frostbite in the structure of sanitary losses during the WWⅡ are studied in this article.

关键词： Thermal injury World War Ⅱ Statistics of burns and frostbite Specialized burn centers

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Improving Laser Beam Welding efficiency

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engineering（科研） 2014年第9期6卷 559-571页

作者： mikhail sokolov Antti Salminen Laboratory of Laser Materials Processing Lappeenranta University of Technology Lappeenranta Finland Machine Technology Centre Turku Ltd Turku Finland

Laser beam welding is becoming widely used in many industrial applications. This paper reviews recent research conducted on the performance, potential and problems of thick section butt joint laser welding. Common defects that occur in laser beam welding with high power laser welding are discussed and possible solutions proposed. Methods of welding process efficiency improvement are analyzed.

关键词： Laser Welding edge Preparation Preheating Vacuum

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Wolf phase tomography(WPT) of transparent structures using partially coherent illumination

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Light(Science & Applications) 2020年第1期9卷 656-664页

作者： Xi Chen mikhail e.Kandel Chenfei Hu Young Jae Lee Gabriel Popescu Quantitative Light Imaging Laboratory Beckman Institute for Advanced Science and TechnologyDepartment of Electrical and Computer EngineeringUniversity of Illinois at Urbana-ChampaignUrbanaIL 61801USA

In 1969,emil Wolf proposed diffraction tomography using coherent holographic imaging to extract 3D information from transparent,inhomogeneous *** the same era,the Wolf equations were first used to describe the propagation correlations associated with partially coherent *** these two concepts,we present Wolf phase tomography(WPT),which is a method for performing diffraction tomography using partially coherent *** reconstruction works directly in the space-time domain,without the need for Fourier transformation,and decouples the refractive index(RI)distribution from the thickness of the *** demonstrate the WPT principle using the data acquired by a quantitative-phase-imaging method that upgrades an existing phase-contrast microscope by introducing controlled phase shifts between the incident and scattered *** illumination field in WPT is partially spatially coherent(emerging from a ring-shaped pupil function)and of low temporal coherence(white light),and as such,it is well suited for the Wolf *** three intensity measurements corresponding to different phase-contrast frames,the 3D RI distribution is obtained immediately by computing the Laplacian and second time derivative of the measured complex correlation *** validate WPT with measurements of standard samples(microbeads),spermatozoa,and live neural *** high throughput and simplicity of this method enables the study of 3D,dynamic events in living cells across the entire multiwell plate,with an RI sensitivity on the order of 10^(−5).

关键词： WPT coherent illumination

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NeCAB family of neuronal calcium-binding proteins in health and disease

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Neural Regeneration Research 2025年第5期20卷 1236-1243页

作者： Diones Bueno Michael K.e.Schafer Sudena Wang Michael J.Schmeisser Axel Methner Institute for Molecular Medicine University Medical Center of the Johannes Gutenberg-UniversityMainzGermany Department of Anesthesiology University Medical Center of the Johannes Gutenberg-UniversityMainzGermany Institute of Anatomy University Medical Center of the Johannes Gutenberg-UniversityMainzGermany

The N-terminal eF-hand calcium-binding proteins 1–3(NeCAB1–3) constitute a family of predominantly neuronal proteins characterized by the presence of at least one eF-hand calcium-binding domain and a functionally less well characterized C-terminal antibiotic biosynthesis monooxygenase domain. All three family members were initially discovered due to their interactions with other proteins. NeCAB1 associates with synaptotagmin-1, a critical neuronal protein involved in membrane trafficking and synaptic vesicle exocytosis. NeCAB2 interacts with predominantly striatal G-protein-coupled receptors, while NeCAB3 partners with amyloid-β A4 precursor protein-binding family A members 2 and 3, key regulators of amyloid-β production. This demonstrates the capacity of the family for interactions with various classes of proteins. NeCAB proteins exhibit distinct subcellular localizations: NeCAB1 is found in the nucleus and cytosol, NeCAB2 resides in endosomes and the plasma membrane, and NeCAB3 is present in the endoplasmic reticulum and Golgi apparatus. The antibiotic biosynthesis monooxygenase domain, an evolutionarily ancient component, is akin to atypical heme oxygenases in prokaryotes but is not wellcharacterized in vertebrates. Prokaryotic antibiotic biosynthesis monooxygenase domains typically form dimers, suggesting that calcium-mediated conformational changes in NeCAB proteins may induce antibiotic biosynthesis monooxygenase domain dimerization, potentially activating some enzymatic properties. However, the substrate for this enzymatic activity remains uncertain. Alternatively, calcium-mediated conformational changes might influence protein interactions or the subcellular localization of NeCAB proteins by controlling the availability of protein–protein interaction domains situated between the eF hands and the antibiotic biosynthesis monooxygenase domain. This review summarizes what is known about genomic organization, tissue expression, intracellular localization, interac

关键词： calcium eF hand mitochondria nervous system signal transduction

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Competing magnetic states in silicene and germanene 2D ferromagnets

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Nano Research 2020年第12期13卷 3396-3402页

作者： Dmitry V.Averyanov Ivan S.sokolov mikhail S.Platunov Fabrice Wilhelm Andrei Rogalev Pierluigi Gargiani Manuel Valvidares Nicolas Jaouen Oleg e.Parfenov Alexander N.Taldenkov Igor A.Karateev Andrey M.Tokmachev Vyacheslav G.Storchak National Research Center“Kurchatov Institute” Kurchatov Sq.1Moscow123182Russia eSRF-The european Synchrotron CS 4022038043Grenoble Cedex 9France ALBA Synchrotron Light Source Cerdanyola del Vallès08290BarcelonaSpain L’Orme des Merisiers Synchrotron SOLEILGyf-sur-Yvette91192France

Two-dimension(2D)magnets have recently developed into a class of stoichiometric materials with prospective applications in ultra-compact spintronics and quantum *** functionality is particularly rich when different magnetic orders are competing in the same *** ReX2(Re=eu,Gd;X=Si,Ge),silicene or germanene—heavy counterparts of graphene—coupled with a layer of rare-earth metals,evolve from three-dimension(3D)antiferromagnets in multilayer structures to 2D ferromagnets in a few *** evolution,however,does not lead to fully saturated 2D ferromagnetism,pointing at a possibility of coexisting/competing magnetic ***,ReX2 magnetism is explored with element-selective X-ray magnetic circular dichroism(XMCD).The measurements are carried out for GdSi2,euSi2,GdGe2,and euGe2 of different thicknesses down to 1 monolayer employing K absorption edges of Si and Ge as well as M and L edges of the *** access the magnetic state in ReX2 and determine the seat of magnetism,orbital,and spin contributions to the magnetic ***-field measurements probe remnants of the bulk antiferromagnetism in 2D *** results provide a new platform for studies of complex magnetic structures in 2D materials.

关键词： silicene germanene 2D ferromagnetism X-ray magnetic circular dichroism(XMCD) monolayer

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The current status and future development of high-temperature conventional superconductivity

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National Science Review 2024年第7期11卷 18-20页

作者： mikhail I.eremets High Pressure Chemistry and Physics Group Max-Planck-Institut für Chemie

This survey highlights key advancements in high-temperature superconductivity in hydrogen-rich materials, emphasizing the robust evidence and reproducibility of superconductivity under challenging experimental conditions of megabar pressures. In the near future, achieving room-temperature superconductivity is highly probable, and the field is expected to transition towards near-ambient-pressure superconductivity.

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Recent Advancements in the Optimization Capacity Configuration and Coordination Operation Strategy of Wind-Solar Hybrid Storage System

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energy engineering 2025年第1期122卷 285-306页

作者： Hongliang Hao Caifeng Wen Feifei Xue HaoQiu Ning Yang Yuwen Zhang Chaoyu Wang edwin e.Nyakilla Department of New energy Storage Peking University Ordos Research Institute of EnergyOrdos017010China School of energy and Power engineering Inner Mongolia University of TechnologyHohhot010080China Key Laboratory of Wind energy and Solar energy Utilization Technology Inner Mongolia University of TechnologyHohhot010051China

Present of wind power is sporadically and cannot be utilized as the only fundamental load of energy *** paper proposes a wind-solar hybrid energy storage system(HeSS)to ensure a stable supply grid for a longer period.A multi-objective genetic algorithm(MOGA)and state of charge(SOC)region division for the batteries are introduced to solve the objective function and configuration of the system capacity,***/Simulink was used for simulation *** optimization results show that for a 0.5 MW wind power and 0.5 MW photovoltaic system,with a combination of a 300 Ah lithium battery,a 200 Ah lead-acid battery,and a water storage tank,the proposed strategy reduces the system construction cost by approximately 18,000 ***,the cycle count of the electrochemical energy storage systemincreases from4515 to 4660,while the depth of discharge decreases from 55.37%to 53.65%,achieving shallow charging and discharging,thereby extending battery life and reducing grid voltage fluctuations *** proposed strategy is a guide for stabilizing the grid connection of wind and solar power generation,capability allocation,and energy management of energy conservation systems.

关键词： electric-thermal hybrid storage modal decomposition multi-objective genetic algorithm capacity optimization allocation operation strategy

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Slc7a5 regulation of neural development

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Neural Regeneration Research 2021年第10期16卷 1994-1995页

作者： Aidan M.sokolov David M.Feliciano Department of Biological Sciences Clemson UniversityClemsonSCUSA

Membrane transport proteins are appreciated for their ability to transport molecules across biological membranes and have received a renewed focus for their role in nervous system *** such group of transporters are members of the solute carrier(SLC)*** transporters shuttle various molecules,including neurotransmitters,fatty acids,amino acids,and inorganic *** such,they play an integral role in supplying cells with essential *** example is that amino acids are transported by SLC family ***7a5 is a SLC family member that transports essential amino acids important for neuronal development,as well as other biological molecules including thyroid hormones T3,T4,and ***7a5 forms a heterodimer with Slc3a2 that facilitates the subcellular distribution of *** acids including leucine,glutamine,and arginine support cell growth by activating the protein kinase,mammalian target of rapamycin complex 1(mTORC1)(Liu et al.,2020).Consistent with this notion is that Slc7a5 transport of amino acids regulates mTORC1 activity in numerous cell types(Nicklin et al.,2009).A recent manuscript expands upon these findings to demonstrate that Slc7a5 plays a previously unrecognized and critical role in the regulation of mTORC1 activity and neuron development(sokolov et al.,2020).

关键词： inorganic solute notion

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extreme Climate and Absence from Work:evidence from Jamaica

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International Journal of Disaster Risk Science 2021年第2期12卷 232-239页

作者： Nekeisha Spencer mikhail-Ann Urquhart Department of economics University of the West IndiesKingstonJamaica

This study investigated the impact of extreme climate events on work absence in *** this end,we constructed a quarterly individual level dataset on labor market and climatic data for *** find that while excess rainfall increases the odds of being temporarily absent from work,heat is unlikely to have an *** estimated outcome of excess rainfall is reasonable given the possibility of flooded roads,which can impede travel to *** draws attention to the development of e-commuting policies to mitigate any negative effects on productivity.

关键词： excess rainfall Heat Jamaica Worker absence

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