Wolf phase tomography(WPT) of transparent structures using partially coherent illumination
作者机构：Quantitative Light Imaging LaboratoryBeckman Institute for Advanced Science and TechnologyDepartment of Electrical and Computer EngineeringUniversity of Illinois at Urbana-ChampaignUrbanaIL 61801USA
出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)
年 卷 期：2020年第9卷第1期
页 面：656-664页
核心收录：
学科分类：070207[理学-光学] 07[理学] 08[工学] 0803[工学-光学工程] 0702[理学-物理学]
基 金：supported by the National Science Foundation(CBET0939511 STC,NRT-UtB 1735252) the National Institute of General Medical Sciences(GM129709) the National Cancer Institute(CA238191)
主 题：WPT coherent illumination
摘 要：In 1969,Emil Wolf proposed diffraction tomography using coherent holographic imaging to extract 3D information from transparent,inhomogeneous *** the same era,the Wolf equations were first used to describe the propagation correlations associated with partially coherent *** these two concepts,we present Wolf phase tomography(WPT),which is a method for performing diffraction tomography using partially coherent *** reconstruction works directly in the space-time domain,without the need for Fourier transformation,and decouples the refractive index(RI)distribution from the thickness of the *** demonstrate the WPT principle using the data acquired by a quantitative-phase-imaging method that upgrades an existing phase-contrast microscope by introducing controlled phase shifts between the incident and scattered *** illumination field in WPT is partially spatially coherent(emerging from a ring-shaped pupil function)and of low temporal coherence(white light),and as such,it is well suited for the Wolf *** three intensity measurements corresponding to different phase-contrast frames,the 3D RI distribution is obtained immediately by computing the Laplacian and second time derivative of the measured complex correlation *** validate WPT with measurements of standard samples(microbeads),spermatozoa,and live neural *** high throughput and simplicity of this method enables the study of 3D,dynamic events in living cells across the entire multiwell plate,with an RI sensitivity on the order of 10^(−5).
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