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Non-redundant Contribution of the Plastidial FAD8 ω-3 Desatumse to Glycerolipid Unsatumtion at Different Temperatures in Arabidopsis

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Molecular Plant 2015年第11期8卷 1599-1611页

作者： angela Roman Maria L. Hernandez angel Soria-Garcia Sara Lopez-Gomollon Beatriz Lagunas Rafael Picorel Jose Manuel Martinez-Rivas miguel Alfonso Estacion Experimental de Aula Dei (EEAD-CSIC) Avda. Montanana 1005 50059 Zaragoza Spain Instituto de la Grasa (IG-CSIC) Campus Universidad Pablo de Olavide Building 46 Ctra. Utrera kin 1 41013 Seville Spain

Plastidial ω-3 desaturase FAD7 is a major contributor to trienoic fatty acid biosynthesis in the leaves of Arabidopsis plants. However, the precise contribution of the other plastidial ω-3 desaturase, FAD8, is poorly understood. Fatty acid and lipid analysis of several ω-3 desaturase mutants, including two insertion lines of AtFAD7 and AtFAD8, showed that FAD8 partially compensated the disruption of the AtFAD7 gene at 22℃, indicating that FAD8 was active at this growth temperature, contrasting to previous observations that circumscribed the FAD8 activity at low temperatures. Our data revealed that FAD8 had a higher selectivity for 18：2 acyl-lipid substrates and a higher preference for lipids other than galactolipids, particularly phosphatidylglycerol, at any of the temperatures studied. Differences in the mechanism controlling AtFAD7 and AtFAD8 gene expression at different temperatures were also detected. Confocal microscopy and biochemical analysis of FAD8-YFP over-expressing lines confirmed the chloroplast envelope localization of FAD8. Co-localization experiments suggested that FAD8 and FAD7 might be located in close vicinity in the envelope membrane. FAD8-YFP over-expressing lines showed a specific increase in 18：3 fatty acids at 22℃. Together, these results indicate that the function of both plastidial ω-3 desaturases is coordinated in a non-redundant manner.

关键词： Arabidopsis thaliana glycerolipid fatty acid FAD7 FAD8 plastid

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Heterostructure engineering in electrode materials for sodium-ion batteries:Recent progress and perspectives

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eScience 2023年第5期3卷 1-11页

作者： Eric Gabriel Chunrong Ma Kincaid Graff angel Conrado Dewen Hou Hui Xiong Micron School of Materials Science and Engineering Boise State UniversityBoiseID83725USA X-ray Sciences Division Argonne National LaboratoryLemontIL60439USA College of Textiles&Clothing Qingdao UniversityQingdao266071China Key Laboratory of Bio-Fibers and Eco-Textiles Qingdao UniversityQingdao266071China Center for Nanoscale Materials Argonne National LaboratoryLemontIL60439USA Center for Advanced Energy Studies Idaho FallsID83401USA

Sodium-ion batteries(SIBs)have stepped into the spotlight as a promising alternative to lithium-ion batteries for large-scale energy storage ***,SIB electrode materials,in general,have inferior performance than their lithium counterparts because Nat is larger and heavier than *** engineering is a promising strategy to overcome this intrinsic limitation and achieve practical *** provide a brief review of recent progress in heterostructure engineering of electrode materials and research on how the phase interface influences Nat storage and transport *** strategies for the design and fabrication of heterostructures(in situ methods)are discussed,with a focus on the heterostructure formation *** heterostructure's influence on Nat storage and transport properties arises primarily from local distortions of the structure and chemomechanical coupling at the phase interface,which may accelerate ion/electron diffusion,create additional active sites,and bolster structural ***,we offer our perspectives on the existing challenges,knowledge gaps,and opportunities for the advancement of heterostructure engineering as a means to develop practical,highperformance sodium-ion batteries.

关键词： Heterostructure Sodium-ion batteries Electrode materials Heterogeneous materials Interface engineering Intergrowth

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Neutralizing possibilities of whole virion and mRNA vaccine triggered antibodies of Wuhan strain of SARS-CoV-2 with receptor binding domains of spike proteins of Delta and Omicron strains

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Asian Pacific Journal of Tropical Medicine 2023年第2期16卷 92-94,I0008页

作者： Shilpa Barthwal Bennet angel Annette angel Vinod Joshi BM Shareef Nuzhat M Peer Poorna Khaneja Ambreen S Khan Bhawna Sharma Neha Singh Ramesh Joshi Komal Tomar Kiran Yadav Satendra P Singh Advance Centre for Virology&Immunology Sharda UniversityGreater NoidaU.P.201310India Sharda School of Agricultural Sciences Sharda UniversityGreater NoidaU.P.201310India Department of Microbiology School of Medical Sciences&ResearchSharda HospitalGreater NoidaU.P.201310India

Mutations in the receptor binding domain(RBD)of SARS-CoV-2 spike protein have given birth to the new variants of concern *** and Omicron *** we are using vaccines which are either mRNA of spike protein of Wuhan strain or its whole virion vaccine,these vaccines will trigger antibodies to be able to bind with the epitopes of spike protein of Wuhan *** appears a need to study whether vaccine generated circulating neutralizing antibodies in vaccinated people are able to neutralize the receptor binding domain of spike protein of currently circulating Delta and Omicron strains as *** need to address this contention in two phases:first to undertake docking studies of vaccine generated antibodies with Wuhan,Delta and Omicron strains and second to conduct the wet lab studies and observe antigen-antibody bindings employing Immunofluorescence and Cryo-Electron Microscopic *** present paper reports results of docking studies using bioinformatic softwares to provide the baseline information for wet lab studies.

关键词： vaccine antibodies neutral

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Optimal control of natural resources in mining industry

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International Journal of Mining Science and Technology 2015年第2期25卷 193-198页

作者： Petrov Nikolay Tanev angel Electrical Engineering Electronics and AutomationTechnical University-Sofia Faculty of Mathematics and Informatics Sofia University

The paper focuses on the optimal control of natural resources in mining *** purpose is to propose an optimal extraction series of these resources during the lifetime of the Mine's *** the proposed optimal control model,a sensitivity analysis has been performed that includes the interest rate impact on the optimal *** study shows that the increasing of the interest rate stimulates faster extraction of the *** discounting factor induces that the resource has to be extracted faster but this effect is counterbalanced by the diminishing returns of the annual cash *** higher parameters of ‘‘alpha'' close to one of the power function about 80% from the whole resource will be extracted during the first 4 years of object/mine *** existence of unique positive root with respect to return of investment has been proposed and proved by two ways:by the ‘‘method of chords' ' and by using specialized software.

关键词：最优控制模型资源开采自然灵敏度分析生命周期提取最优解现金流

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Hydatid cyst of the colon:A systematic review of the literature

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World Journal of Clinical Cases 2019年第13期7卷 1634-1642页

作者： miguel angel Latatu-Córdoba Sergio Ruiz-Blanco Matilde Sanchez Carmen Santiago-Boyero Paula Soto-García Wenzhong Sun José M Ramia Faculty of Medicine Universidad of Alcalá Alcalá de Henares 28801 Spain Servicio de Cirugía Generaly del Aparato Digestivo Hospital Universitario de Guadalajara Guadalajara 19002 Spain

BACKGROUND Primary hydatid disease in the colon is an extremely rare finding. We performed a systematic review of cases of hydatidosis of the colon published to date. AIM To systematic review the published papers about hydatidosis of the colon. METHODS Following PRISMA guidelines, we performed an unlimited search for articles on CH in the MEDLINE (PubMed), EMBASE, Cochrane, Latindex and Scielo databases, published in English, Spanish, French, German or Italian between January 1, 1960 and December 15, 2018. The search items were:(Colon) AND [(hydatidosis) OR (hydatid disease) OR (echinococcosis)]. RESULTS Nine case reports were found [five men, four women, median age 64.55 years (range: 21-81)]. The most frequent clinical manifestation was abdominal pain (8/9)(88.9%). In almost all cases several image studies were carried out, but abdominal computed tomography was the most used (66.7%). Five resections of the colon (62.5%) and three sutures of the fistula between cyst and colon (37.5%) were performed. Four patients did not present post-operative complications, but one died. Postoperative period was recorded for a mean period of 35 months. No recurrences were recorded. CONCLUSION The most frequent symptom was abdominal pain (evidence level 4). Computed tomography was the most frequently used diagnostic imaging technique (evidence level 4). Colectomy was the most appropriate treatment (evidence level 5). All the diagnostic and therapeutic options for hydatid cysts located in colon have a low level of evidence.

关键词： Echinococcosis Hydatidosis Hydatid cyst Colon Surgery Review

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High-Rise Residential Reinforced Concrete Building Optimisation

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Open Journal of Civil Engineering 2015年第4期5卷 437-450页

作者： Haibei Xiong miguel angel Hidalgo Calvo Department of Structural Engineering Tongji University Shanghai China Department of Civil Engineering Polytechnic University of Catalonia/Tongji University (UPC) Barcelona Spain

In the last few decades structure optimisation has become a main task in a civil engineering project. As a matter of fact, due to the complexity and particularity of every structure, the great amount of variables and design criteria to considerate and many other factors, a general optimisation’s method is not simple to formulate. As a result, this paper focuses on how to provide a successful optimisation method for a particular building type, high-rise reinforced concrete buildings. The optimization method is based on decomposition of the main structure into substructures: floor system, vertical load resisting system, lateral load resisting system and foundation system;then each of the subsystems using the design criteria established at the building codes is improved. Due to the effect of the superstructure optimisation on the foundation system, vertical and lateral load resisting system is the last to be considered after the improvement of floor. Finally, as a case example, using the method explained in the paper, a 30-story-high high-rise residential building complex is analysed and optimised, achieving good results in terms of structural behaviour and diminishing the overall cost of the structure.

关键词： Structure Optimisation High-Rise Residential Reinforced Concrete Buildings Shear-Wall Structure Deep Piles Post Grouting

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Colorectal cancer population screening programs worldwide in 2016: An update

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World Journal of Gastroenterology 2017年第20期23卷 3632-3642页

作者： Mercedes Navarro Andrea Nicolas angel Ferrandez angel Lanas Service of Digestive Diseases University Clinic Hospital Lozano Blesa University of Zaragoza Aragón Health Research Institute (IIS Aragón) CIBERehd

Colorectal cancer(CRC)is the third most commonly diagnosed cancer in the *** incidence and mortality show wide geographical *** is recommended to reduce both incidence and ***,there are significant differences among studies in implementation strategies and *** review aimed to present the results and strategies of different screening programs *** reviewed the literature on national and international screening programs published in Pub Med,on web pages,and in clinical *** Screening programs are currently underway in most European countries,Canada,specific regions in North and South America,Asia,and *** most extensive screening strategies were based on fecal occult blood testing,and more recently,the fecal immunochemical test(FIT).Participation in screening has varied greatly among different *** Netherlands showed the highest participation rate(68.2%)and some areas of Canada showed the lowest(16%).Participation rates were highest among women and in programs that used the FIT *** exhibited the greatest number of positive *** FIT test has been the most widely used screening program *** advent of this test has increased participation rates and the detection of positive results.

关键词： Colorectal 癌症屏蔽的 Colorectal 癌症烘便的秘密验血烘便的免疫化学的测试 Colonoscopy

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Habitat use and space preferences of Eurasian Bullfinches(Pyrrhula pyrrhula)in northwestern Iberia throughout the year

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Avian Research 2021年第1期12卷 37-51页

作者： angel Hernández Departamento de Ciencias Agroforestales Area de ZoologiaUniversidad de ValladolidCampus de PalenciaPalenciaEspana Instituto Universitario de Investigacion en Gestion Forestal Sostenible Universidad de ValladolidCampus de PalenciaPalenciaEspana

Background:For all vertebrates in general,a concerted effort to move beyond single season research is vital to improve our understanding of species *** of habitat use and selection by Eurasian Bullfinches(Pyrrhula pyrrhula)is limited with regard to the non-breeding *** date,research on the habitat of the Iberian subspecies iberiae consists of very general *** relation to space use,only broad features are available for the entire distribution range of Eurasian Bullfinches,including ***:In this study,seasonal preferences regarding habitat and space in a population of Eurasian Bullfinches are examined for the first time in the Iberian Peninsula,through direct observation during a six-year *** essential habitat components,substrate selection and perch height were ***:Hedgerows were the key essential habitat component for bullfinches during all ***,small poplar plantations became increasingly important from winter to *** perched mostly in shrubs/trees throughout the year,but there were significant seasonal changes in substrate use,ground and herbs being of considerable importance during *** the year,over half of the records corresponded to feeding,reaching almost 90%in ***,bullfinches perched noticeably lower while *** bullfinches perched markedly higher than females,notably singing males in *** perched at a height not much lower than that of *** all seasons,males tended to feed at greater heights than *** of different ages and sexes were seen bathing in all seasons except ***:Hedgerow habitat in general appeared to be valuable for bullfinches throughout the *** summer and autumn,they selected sites with an abundance of food and shade,as well as shelter,a much-needed requirement for fledglings and moulting *** was usually a close link between the most used

关键词： Habitat use Perch height Pyrrhula pyrrhula iberiae Seasonal variation Substrate selection Water bathing

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Friction-based riveting technique for AZ31 magnesium alloy

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Journal of Magnesium and Alloys 2022年第1期10卷 122-130页

作者： Tianhao Wang Scott Whalen Xiaolong Ma Joshua Silverstein Hrishikesh Das Madhusudhan R.Pallaka angel Ortiz Timothy Roosendaal Piyush Upadhyay Keerti S Kappagantula Pacific Northwest National Laboratory MSIN:K2-03902 Battelle Blvd.RichlandWA 99352United States

A new friction-based riveting technique, Rotating Hammer Riveting(RHR), is demonstrated to fully form AZ31 Mg rivet heads in a mere 0.23 s. Heat and pressure generated through severe plastic deformation during the process was sufficient to form the Mg rivet head without the need for a pre-heating operation. Due to preliminary twinning and followed by dynamic recrystallization, AZ31 Mg grains in the rivet head were refined during RHR, which enhance the formability of Mg rivets by triggering grain boundary sliding and reducing plastic anisotropy of Mg. In addition, RHR joints showed a metallurgical bond between the rivet head and top AZ31 Mg sheet, which eliminates a significant pathway for corrosion.

关键词： Rivet Magnesium Dissimilar joining

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Proteomic analysis of F_(1)hybrids and intermediate variants in a Littorina saxatilis hybrid zone

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Current Zoology 2022年第3期68卷 351-359页

作者： angel P.DIZ Mónica R.ROMERO Juan GALINDO María SAURA David O.F.SKIBINSKI Emilio ROLÁN-ALVAREZ Centro de Investigación Mariña(CIM-UVIGO) Universidade de VigoVigo 36310Spain Departamento de Mejora Genética Animal INIAMadrid 28040Spain Institute of Life Science Swansea University Medical SchoolSwansea SA28PPUK

Proteomic analysis was carried out on the Crab(upper-shore)and Wave(lower-shore)ecotypes of Littorina saxatilis from a hybrid zone at Silleiro Cape,*** profiles of individual snails were *** expression in F_(1)hybrid snails bred in the laboratory and snails with intermediate shell phenotypes collected from the mid-shore were compared with Crab and Wave ecotypes using analytical approaches used to study *** analysis over many protein spots showed that the F_(1)snails are distinct from both ecotypes but closer to the Wave *** intermediate snails are highly variable,some closer to the Crab and others to the Wave *** on a protein by protein basis,some proteins are significantly closer in expression to the Crab and others to the Wave ecotype for both F_(1)and intermediate ***,a significant majority of proteins were closer in expression to the Wave ecotype for the F_(1),consistent with the multivariate *** such significant majority toward either the Crab or Wave ecotype was observed for the intermediate *** closer similarity of F_(1)and Wave ecotype expression patterns could be the result of similar selective pressures in the similar mid-shore and low-shore *** a significantly larger number of proteins,intermediate snails were closer in expression to the ecotype having the lower expression,for both Crab and Wave *** is somewhat unexpected as lower expression might be expected to be an indication of impairment of function and lower *** analysis could be important for the identification of candidate proteins useful for gaining improved understanding of adaptation and barriers to gene flow in hybrid zones.

关键词： F_(1)hybrids gene expression marine invertebrates molecular phenotype reproductive isolation speciation

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