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Competitive interactions and coexistence of sympatric flagship carnivores in Asia

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Integrative Zoology 2024年第2期19卷 183-199页

作者： Zhilin LI Jiayu LU Xiaoyi SHI Li’an dUO James L.d.smith Tianming WANG Tianjin Key Laboratory of Conservation and Utilization of Animal diversity College of Life SciencesTianjin Normal UniversityTianjinChina department of Fisheries Wildlife and Conservation BiologyUniversity of MinnesotaSt.PaulMinnesotaUSA Ministry of Education Key Laboratory for Biodiversity Science and Engineering College of Life SciencesBeijing Normal UniversityBeijingChina National Forestry and Grassland.a.ministration Key Laboratory for Conservation Ecology of Northeast Tiger and Leopard BeijingChina

Understanding the competition and coexistence of flagship carnivores is key to creating strategies for their conservation in the face of global carnivore *** studies exploring the dynamics and competition between tigers(Panthera tigris)and leopards(***)span decades,there is a lack of understanding regarding the factors that influence their coexistence mechanisms on a broad scale,as well as the drivers determining their exploitative and interference *** gathered.a.comprehensive list of research papers among which 36 papers explored the interspecific interactions between tigers and leopards and tested the influence of biotic and.a.iotic factors on the coexistence mechanisms along three dimensions using multiple response variables regression models;we also tested the influence of ecological drivers determining the exploitative or interference competition between tigers and *** and ungulate density were the most important predictors in regulating the coexistence *** and leopards exhibited more positive relations/higher overlaps as elevation increased in the spatial *** addition,they showed.a.higher dietary overlap in the prey-rich *** determined that interference competition between tigers and leopards was less frequently observed in habitats with dense tree cover and homogeneous vegetation ***,studies with multiple metrics would promote the detection of interference *** study provides new insight into the competitive interactions and coexistence mechanisms of tigers and leopards on a broad ***-makers and managers should pay more attention to the factors of elevation,prey abundance,and habitat structures for the conservation of tigers and leopards.

关键词： carnivores coexistence mechanisms competitive interactions exploitative competition interference competition

Facile synthesis of intra-nanogap enhanced Raman tags with different shapes

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Nano Research 2024年第9期17卷 8415-8423页

作者： Sanjun Fan Brian T.Scarpitti Zhewen Luo Abigail E.smith Jian Ye Zachary d.Schultz department of Chemistry and Biochemistry The Ohio State UniversityColumbusOhio 43210USA School of Biomedical Engineering Shanghai Jiao Tong UniversityShanghai 200030China

Hot spot engineering in plasmonic nanostructures plays a significant role in surface-enhanced Raman scattering(SERS)for bioanalysis and cell ***,creating stable,reproducible,and strong SERS signals remains challenging due to the potential interference from surrounding chemicals and locating SERS-active analytes into hot-spot ***,we developed.a.straightforward.a.proach to synthesize intra-gap nanoparticles encapsulating 4-nitrobenzenethiol(4-NBT)as a reporter molecule within these gaps to avoid outside *** made three kinds of intra-gap nanoparticles using nanorods,bipyramids,and nanospheres as cores,in which the nanorods based intra-gap nanoparticles exhibit the highest SERS *** ad.a.tage of our method is the ease of preparation of high-yield.a.d stable intra-gap nanoparticles characterized by a short incubation time(10 min)with 4-NBT and quick synthesis without requiring an additional step to centrifuge for the purification of core *** intense localized field in the synthesized hot spots of these plasmonic gap nanostructures holds great promise as a SERS substrate for a broad range of quantitative optical applications.

关键词： hot spot engineering surface enhanced Raman scattering intra-gap nanoparticles 4-nitrobenzenethiol

Aerosol jet printing of surface acoustic wave microfluidic devices

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Microsystems & Nanoengineering 2024年第1期10卷 231-242页

作者： Joseph Rich Brian Cole Teng Li Brandon Lu Hanyu Fu Brittany N.smith Jianping Xia Shujie Yang Ruoyu Zhong James L.doherty Kanji Kaneko Hiroaki Suzuki Zhenhua Tian Aaron d.Franklin Tony Jun Huang department of Biomedical Engineering Duke UniversityDurhamNC 27708USA department of Electrical and Computer Engineering Duke UniversityDurhamNC 27708USA department of Mechanical Engineering Virginia Polytechnic Institute and State UniversityBlacksburgVA 24061USA Thomas Lord department of Mechanical Engineering and Materials Science Duke UniversityDurhamNC 27708USA deptartment of Precision Mechanics Faculty of Science and EngineeringChuo UniversityTokyo 112-8551Japan department of Chemistry Duke UniversityDurhamNC 27708USA

The addition of surface acoustic wave(SAW)technologies to microfluidics has greatly ad.a.ced lab-on-a-chip applications due to their unique and powerful attributes,including high-precision manipulation,versatility,integrability,biocompatibility,contactless nature,and rapid ***,the development of SAW microfluidic devices is limited by complex and time-consuming micro/nanofabrication techniques and.a.cess to cleanroom facilities for multistep photolithography and vacuum-based *** simplify the fabrication of SAW microfluidic devices with customizable dimensions and functions,we utilized the additive manufacturing technique of aerosol jet *** successfully fabricated customized SAW microfluidic devices of varying materials,including silver nanowires,graphene,and poly(3,4-ethylenedioxythiophene)polystyrene sulfonate(PEdOT:PSS).To characterize and compare the acoustic actuation performance of these aerosol jet printed SAW microfluidic devices with their cleanroom-fabricated counterparts,the wave displacements and resonant frequencies of the different fabricated devices were directly measured through scanning laser doppler ***,to exhibit the capability of the aerosol jet printed devices for lab-on-a-chip applications,we successfully conducted.a.oustic streaming and particle concentration ***,we demonstrated.a.novel solution-based,direct-write,single-step,cleanroom-free additive manufacturing technique to rapidly develop SAW microfluidic devices that shows viability for applications in the fields of biology,chemistry,engineering,and medicine.

关键词： printing wave fluid

Genetic interactions between polycystin-1 and Wwtr1 in osteoblasts define a novel mechanosensing mechanism regulating bone formation in mice

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Bone Research 2023年第4期11卷 772-783页

作者： Zhousheng Xiao Li Cao micholas d.a. smith Hanxuan Li Wei Li Jeremy C.smith Leigh darryl Quarles department of Medicine University of Tennessee Health Science CenterMemphisTN 38163USA UT/ORNL Center for Molecular Biophysics Oak Ridge National LaboratoryOak RidgeTN 37830USA department of Biochemistry and Cellular and Molecular Biology The University of Tennessee-KnoxvilleKnoxvilleTN 37996-1939USA department of Pharmaceutical Sciences University of Tennessee Health Science CenterMemphisTN 38163USA

Molecular mechanisms transducing physical forces in the bone microenvironment to regulate bone mass are poorly ***,we used mouse genetics,mechanical loading,and pharmacological approaches to test the possibility that polycystin-1 and Wwtr1 have interdependent mechanosensing functions in *** created.a.d compared the skeletal phenotypes of control Pkd1^(flox/)+;Wwtr1^(flox/)+,Pkd1^(Oc-cKO),Wwtr1^(Oc-cKO),and Pkd1/Wwtr1^(Oc-cKO)mice to investigate genetic *** with an interaction between polycystins and Wwtr1 in bone in vivo,Pkd1/Wwtr1^(Oc-cKO)mice exhibited greater reductions of BMd.a.d periosteal MAR than either Wwtr1Oc-cKOor Pkd1^(Oc-cKO)***-CT 3d image analysis indicated that the reduction in bone mass was due to greater loss in both trabecular bone volume and cortical bone thickness in Pkd1/Wwtr1Oc-cKO mice compared to either Pkd1Oc-cKOor Wwtr1^(Oc-cKO)***1/Wwtr1^(Oc-cKO)mice also displayed.a.ditive reductions in mechanosensing and osteogenic gene expression profiles in bone compared to Pkd1Oc-cKOor Wwtr1^(Oc-cKO)***,we found that Pkd1/Wwtr1^(Oc-cKO)mice exhibited impaired responses to tibia mechanical loading in vivo and.a.tenuation of loadinduced mechanosensing gene expression compared to control ***,control mice treated with a small molecule mechanomimetic,MS2 that activates the polycystin complex resulted in marked increases in femoral BMd.a.d periosteal MAR compared to vehicle *** contrast,Pkd1/Wwtr1^(Oc-cKO)mice were resistant to the anabolic effects of *** findings suggest that PC1 and Wwtr1 form an anabolic mechanotransduction signaling complex that med.a.es mechanical loading responses and serves as a potential novel therapeutic target for treating osteoporosis.

关键词： mec impaired additive

A unique subseafloor microbiosphere in the Mariana Trench driven by episodic sedimentation

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Marine Life Science & Technology 2024年第1期6卷 168-181页

作者： Jiwen Liu da-Wei Li Xinxin He Ronghua Liu Haojin Cheng Chenglong Su Mengna Chen Yonghong Wang Zhongsheng Zhao Hanyue Xu Zhangyu Cheng Zicheng Wang Nikolai Pedentchouk david J.Lea-smith Jonathan d.Todd Xiaoshou Liu Meixun Zhao Xiao-Hua Zhang Frontiers Science Center for deep Ocean Multispheres and Earth System and College of Marine Life SciencesOcean University of ChinaQingdao 266003China Laboratory for Marine Ecology and Environmental Science Laoshan LaboratoryQingdao 266237China Institute of Evolution and Marine Biodiversity Ocean University of ChinaQingdao 266003China Key Laboratory of Marine Chemistry Theory and Technology Ministry of EducationOcean University of ChinaQingdao 266100China Key Lab of Submarine Geosciences and Prospecting Techniques Ministry of Education/College of Marine GeosciencesOcean University of ChinaQingdao 266100China Key Laboratory of Physical Oceanography Ministry of Education/Research Vessel CentreOcean University of ChinaQingdao 266100China School of Environmental Sciences University of East AngliaNorwich NR47TJUK School of Biological Sciences University of East AngliaNorwich NR47TJUK

Hadal trenches are characterized by enhanced.a.d infrequent high-rate episodic sedimentation events that likely introduce not only labile organic carbon and key nutrients but also new microbes that significantly alter the subseafloor ***,the role of high-rate episodic sedimentation in controlling the composition of the hadal subseafloor microbiosphere is ***,analyses of carbon isotope composition in a~750 cm long sediment core from the Challenger deep revealed noncontinuous deposition,with anomalous ^(14)C ages likely caused by seismically driven mass transport and the funneling effect of trench *** community composition and diverse enzyme activities in the upper~27 cm differed from those at lower depths,probably due to sudden sediment deposition and differences in redox condition and organic matter *** lower depths,microbial population numbers,and composition remained relatively constant,except at some discrete depths with altered enzyme activity and microbial phyla abundance,possibly due to additional sudden sedimentation events of different *** is provided of a unique role for high-rate episodic sedimentation events in controlling the subsurface microbiosphere in Earth’s deepest ocean floor and highlight the need to perform thorough analysis over a large depth range to characterize hadal benthic *** depositional processes are likely crucial in shaping deep-water geochemical environments and thereby the deep subseafloor biosphere.

关键词： Hadal subseafloor deep water sediment Mariana Trench Radiocarbon Microbial community Redox potential

Polyethylene glycol has immunoprotective effects on sciatic allografts, but behavioral recovery and graft tolerance require neurorrhaphy and.a.onal fusion

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Neural Regeneration Research 2025年第4期20卷 1192-1206页

作者： Tyler A.smith Liwen Zhou Cameron L.Ghergherehchi Michelle Mikesh Cathy Z.Yang Haley O.Tucker JuliAnne Allgood Jared S.Bushman George d.Bittner department of Molecular Biosciences University of Texas at AustinAustinTXUSA department of Neuroscience University of Texas at AustinAustinTXUSA division of Pharmaceutical Sciences University of WyomingLaramieWYUSA

Behavioral recovery using(viable)peripheral nerve allografts to repair ablation-type(segmental-loss)peripheral nerve injuries is delayed or poor due to slow and inaccurate axonal ***,such peripheral nerve allografts undergo immunological rejection by the host immune *** contrast,peripheral nerve injuries repaired by polyethylene glycol fusion of peripheral nerve allografts exhibit excellent behavioral recovery within weeks,reduced immune responses,and many axons do not undergo Wallerian *** relative contribution of neurorrhaphy and polyethylene glycol-fusion of axons versus the effects of polyethylene glycol per se was unknown prior to this *** hypothesized that polyethylene glycol might have some immune-protective effects,but polyethylene glycol-fusion was necessary to prevent Wallerian degeneration and functional/behavioral *** examined how polyethylene glycol solutions per se affect functional and behavioral recovery and peripheral nerve allograft morphological and immunological responses in the absence of polyethylene glycol-induced.a.onal ***-type sciatic nerve injuries in outbred Sprague–dawley rats were repaired.a.cording to a modified protocol using the same solutions as polyethylene glycol-fused peripheral nerve allografts,but peripheral nerve allografts were loose-sutured(loose-sutured polyethylene glycol)with an intentional gap of 1–2 mm to prevent fusion by polyethylene glycol of peripheral nerve allograft axons with host *** to negative control peripheral nerve allografts not treated by polyethylene glycol and in contrast to polyethylene glycol-fused peripheral nerve allografts,animals with loose-sutured polyethylene glycol peripheral nerve allografts exhibited Wallerian degeneration for all axons and myelin degeneration by 7 days postoperatively and did not recover sciatic-med.a.ed behavioral functions by 42 days *** morphological signs of rejection,such as col

关键词： allograft rejection axotomy macrophage myelin nerve repair polyethylene glycol(PEG) sciatic nerve T cell transplantation Wallerian degeneration

极端干旱与食草昆虫对草原群落的交互作用受干旱模式的影响

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Journal of Plant Ecology 2024年第3期17卷 128-140页

作者： Ye Luo Yuguang Ke Hongqiang Wang Chong Xu Qian Gu Qiang Yu Melinda d.smith Rajabboy Madrimov Nuriddin Samatov Wei Yang Honghui Wu State Key Laboratory of Effcient Utilization of Arid.a.d Semi-arid.a.able Land in Northern China Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081China Key Laboratory of Arable Land Quality Monitoring and Evaluation Ministry of Agriculture and Rural AffairsInstitute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081China School of Grassland Science Beijing Forestry UniversityBeijing 100083China department of Biology and Grad.a.e degree Program in Ecology Colorado State UniversityFort CollinsCO 80523USA Research Institute of Environment and Nature Conservation Technologies of the Ministry of Ecology Environmental Protectionand Climate Change of the Republic of UzbekistanTashkent 100043Uzbekistan Institute of Microbiology of the Academy of Sciences Tashkent 100128Uzbekistan Institute of Environment and Sustainable development in Agriculture Chinese Academy of Agricultural SciencesBeijing 100081China

极端干旱和食草昆虫可以共同影响植物群落,并且它们的交互作用可能受到干旱模式的影响。然而,目前尚缺乏相关的观测证据。本研究以半干旱草原为研究对象,通过控制实验探讨了不同极端干旱模式与食草昆虫的交互作用对植物群落的影响。实... 详细信息

极端干旱和食草昆虫可以共同影响植物群落,并且它们的交互作用可能受到干旱模式的影响。然而,目前尚缺乏相关的观测证据。本研究以半干旱草原为研究对象,通过控制实验探讨了不同极端干旱模式与食草昆虫的交互作用对植物群落的影响。实验设置了3种干旱处理,分别为对照(自然降雨量)、慢性干旱(生长季5–8月减少66%降雨量)和急性干旱(6–7月完全无降雨)。在各小区设置了两种食草昆虫处理,分别是有食草昆虫和无食草昆虫。结果表明,食草昆虫和极端干旱及二者的交互作用对物种丰富度无显著影响。食草昆虫缓解了慢性干旱对总盖度的负面影响,而这种缓解作用在急性干旱下较低。食草昆虫对慢性干旱带来的负面效应具有更强的缓解作用,这主要是因为食草昆虫导致慢性干旱下适口性较高的杂类草(具有较低叶片碳含量、叶片干物质含量,以及较低叶片淀粉、钙、镁、锰含量)的大幅降低,进而降低了禾草的负面效应。上述结果表明,干旱和食草昆虫的交互作用受干旱模式的影响。通过调节植物的适口性来控制食草昆虫的采食行为,可以减轻极端干旱对草原生态系统的影响。

关键词：急性干旱慢性干旱半干旱草原食草昆虫极端气候

Comparative efficacy and optimal duration of first-line antibiotic regimens for acute otitis med.a.in children and.a.olescents:a systematic review and network meta-analysis of 89 randomized clinicaltrials

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World Journal of Ped.a.rics 2024年第3期20卷 219-229页

作者： Min Seo Kim Jae Han Kim Seohyun Ryu Seung Won Lee dong Keon Yon Eunyoung Kim Ai Koyanagi Elena d.a.ioti Jae Il Shin Lee smith Samsung Ad.a.ced Institute forHealth Sciences&Technology(SAIHST) Samsung Medical CenterSungkyunkwan UniversitySeoulRepublic of Korea Yonsei University Collegeof Medicine SeoulRepublicof Korea department of Precision Medicine Sungkyunkwan University School of MedicineSuwonRepublic of Korea Center for digital Health Medical Science Research InstituteKyung Hee University College of MedicineSeoulRepublic of Korea Evidence-Based Research Laboratory Department of Clinical Pharmacy and Pharmaceutical CareCollege of PharmacyChung-Ang UniversitySeoulRepublic of Korea Parc Sanitari Sant Joan de deu/CIBERSAM/ISCIII Universitat de BarcelonaFundacio Sant Joan de Déu.Sant Boi de LlobregatBarcelonaSpain ICREA Pg.Lluis Companys 23BarcelonaSpain Pain and Rehabilitation Centre and Department of Medical and Health SciencesLinkoping University58185 LinkopingSweden department of Ped.a.rics Yonsei University College of MedicineYonsei-ro 50Seodaemun-gu8044Seoul 03722Republic of Korea Centre for Health Performanceand WellbeingAnglia Ruskin UniversityCambridge CB11PTUK Research Laboratory Psychology of Patients Families&Health ProfessionalsDepartment of NursingSchool of Health SciencesUniversity of IoanninaIoannina 45500Greece

Introduction Antibiotic use for acute otitis med.a.AOM)is one of the major sources of antimicrobial ***,the effective minimal antibiotic duration for AOM remains ***,guidelines often recommend broad ranges(5-10 days)of antibiotic use,yet the clinical impact of such a wide window has not been *** We systematically searched PubMed/MEdLINE,Embase,Scopus,Web of Science,and Cochrane Library from database inception to 6 October *** meta-analysis was conducted on randomized controlled trials that assessed.a.tibiotic treatment for AOM in children(PROSPERO CRd42020196107).Results For amoxicillin and.a.oxicillin-clavulanate,7-day regimens were noninferior to 10-day regimens in clinical responses[amoxicillin:risk ratio(RR)0.919(95%CI 0.820-1.031),amoxicillin-clavulanate:RR 1.108(0.957-1.282)],except for≤2 *** the third-generation cephalosporins,7-day and 10-day regimens had similar clinical responses compared to placebo[7-day:RR 1.420(1.190-1.694),10-day:RR 1.238(1.125-1.362)compared to placebo].However,5-day regimens of amoxicillin-clavulanate and third-generation cephalosporins were inferior to 10-day *** to amoxicillin,a shorter treatment duration was tolerable with *** Our findings indicated that 1O days of antibiotic use may be unnecessarily long,while the treatment duration should be longer than 5 ***,5-day regimens would be sufficient for a modest treatment *** findings revealed that the current wide range of recommended.a.tibiotic durations may have influenced the clinical outcome of AOM,and.a.narrower antibiotic duration window should be re-established.

关键词： Amoxicillin-potassium Amoxicillin Antibacterial agents Cephalosporins duration of therapy

内蒙古草原优势物种光合速率对极端干旱的响应

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Journal of Plant Ecology 2024年第3期17卷 5-15页

作者： Yalong Shi Yuanbo Cao Honghui Wu Chong Xu Qiang Yu Xiaoan Zuo Xingguo Han Melinda d.smith Alan K.Knapp Chengjie Wang Guodong Han State Key Laboratory of Effcient Utilization of Arid.a.d Semi-arid.a.able Land in Northern China Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081China Key Laboratory of Arable Land Quality Monitoring and Evaluation Ministry of Agriculture and Rural AffairsInstitute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081China China Rida Investment and development Group Corp Ltd Beijing 100040China School of Grassland Science Beijing Forestry UniversityBeijing 100083China Urat desert-grassland Research Station Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhou 730000China State Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeijing 100093China department of Biology and Grad.a.e degree Program in Ecology Colorado State UniversityFort CollinsCO 80523USA College of Grassland Resource and EnvironmentalInner Mongolia Agricultural UniversityHohhot 010011China

评估极端干旱对优势植物物种光合速率(Pn)的影响对于我们理解极端干旱影响生态系统功能的机制至关重要。极端干旱可能由降雨量减少或降雨频率减少造成。这两种不同模式的极端干旱可能造成不同的影响。此外,不同草原类型可能对这两种极... 详细信息

评估极端干旱对优势植物物种光合速率(Pn)的影响对于我们理解极端干旱影响生态系统功能的机制至关重要。极端干旱可能由降雨量减少或降雨频率减少造成。这两种不同模式的极端干旱可能造成不同的影响。此外,不同草原类型可能对这两种极端干旱模式表现出不同的敏感性。然而,目前尚不清楚这两种极端干旱模式对草原优势植物物种Pn的影响有何差异,以及在不同草原类型这两种不同模式的极端干旱的效应是否改变。为此,我们于2014年在内蒙古草原两个不同类型的草原(荒漠草原vs.典型草原)站点各进行了一项多年的极端干旱(CHR:将生长季期间每次降雨事件的降雨量减少66%***:完全排除生长季内一段较短时间的降水)模拟实验。于2017年的7月和8月,我们在这两个草原站点各测量了两种优势物种的Pn。结果发现,CHR和INT均显著降低了优势物种的Pn。无论草原类型如何,INT对Pn造成的负面影响都更大。在荒漠草原,极端干旱对Pn的负面影响普遍大于典型草原,尤其是对于CHR处理下的羊草(Leymus chinensis)。这些结果表明,与降雨量减少相比,降雨频率减少所引起的极端干旱对Pn的负面影响更大。草地类型可能会改变极端干旱模式效应的程度,但不会改变其方向。这些发现强调了应对未来极端干旱的草原生态系统管理应考虑极端干旱模式和草地类型的影响。

关键词：极端气候荒漠草原典型草原极端干旱模式季节性干旱