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Mechanical characterization of rose bengal and green light crosslinked collagen scaffolds for regenerative medicine

Regenerative Biomaterials 2021年第6期8卷 185-198页

作者： Joy Braun Stefanie Eckes michelle fiona kilb Dirk Fischer Claudia Eßbach Pol Maria Rommens Philipp Drees Katja Schmitz Daniela Nickel Ulrike Ritz Department of Orthopedics and Traumatology BiomaTiCSUniversity Medical CenterJohannes Gutenberg UniversityLangenbeckstraße 1Mainz 55131Germany Clemens-Schopf-Institute of Organic Chemistry and Biochemistry Technical University of DarmstadtAlarich-Weiss-Straße 4Darmstadt 64287Germany Berufsakademie Sachsen—Staatliche Studienakademie Glauchau University of Cooperative EducationKopernikusstraße 51Glauchau 08371Germany

Collagen is one of the most important biomaterials for tissue engineering *** its excellent biocompatibility,it shows the non-negligible disadvantage of poor mechanical *** crosslinking with rose bengal and green light(RGX)is an appropriate method to improve this *** development of collagen laminates is helpful for further adjustment of the mechanical properties as well as the controlled release of incorporated *** this study,we investigate the impact of crosslinking and layering of two different collagen scaffolds on the swelling behavior andmechanical behavior inmicro tensile tests to obtain information on its wearing comfort(stiffness,strength and ductility).The mechanical stability of the collagen material after degradation due to cell contact is examined using thickness *** is no linear increase or decrease due to layering homologous ***,a decrease in elongation at break,Young’s modulus and ultimate tensile strength are measured when the untreated monolayer is compared to the crosslinked ***,we can detect a connection between stability and cell *** results show that with variation in number and type of layers,collagen scaffolds with tailored mechanical properties can be *** a multi-layered structure enables the release of biomolecules into inner or outer layers for biomedical applications.

关键词： collagen type I collagen laminates rose bengal and green light crosslinking thickness analysis micro tensile testing cell-collagen interactions

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新的病毒性疾病：一个无界限的现实和潜在的问题

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美国医学会杂志（中文版） 1990年第6期9卷 347-352页

作者： kilb.,ED 高伟士

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finDr:A web server for in silico D-peptide ligand identification

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Synthetic and Systems Biotechnology 2021年第4期6卷 402-413页

作者： Helena Engel Felix Guischard Fabian Krause Janina Nandy Paulina Kaas Nico Hofflin Maja Kohn Normann kilb Karsten Voigt Steffen Wolf Tahira Aslan Fabian Baezner Salom´e Hahne Carolin Ruckes Joshua Weygant Alisa Zinina Emir Bora Akmeriç Enoch B.Antwi Dennis Dombrovskij Philipp Franke Klara L.Lesch Niklas Vesper Daniel Weis Nicole Gensch Barbara Di Ventura Mehmet Ali Oztürk Signalling Research Centres BIOSS and CIBSS University of FreiburgSchanzlestr.1879104FreiburgGermany Institute of Biology III Faculty of BiologyUniversity of FreiburgSchanzlestr.179104FreiburgGermany Institute of Biology II Faculty of BiologyUniversity of FreiburgSchanzlestr.179104FreiburgGermany AG Roth-Lab for MicroarrayCopying ZBSA-Centre for Biological Systems AnalysisUniversity of FreiburgHabsburgerstrasse 4979104FreiburgGermany Biomolecular Dynamics Institute of PhysicsUniversity of FreiburgHermann-Herder-Strasse 3a79104FreiburgGermany Core Facility Signalling Factory Centre for Biological Signaling Studies(BIOSS)University of FreiburgSchanzlestr.1879104FreiburgGermany Spemann Graduate School of Biology and Medicine(SGBM) University of FreiburgAlbertstraße 19A79104FreiburgGermany Internal Medicine IV Department of MedicineMedical CenterUniversity of FreiburgHugstetter Straße 5579106FreiburgGermany Institute for Biochemistry University of FreiburgAlbertstr.2179104FreiburgGermany

In the rapidly expanding field of peptide therapeutics,the short in vivo half-life of peptides represents a considerable limitation for drug action.D-peptides,consisting entirely of the dextrorotatory enantiomers of naturally occurring levorotatory amino acids(AAs),do not suffer from these shortcomings as they are intrinsically resistant to proteolytic degradation,resulting in a favourable pharmacokinetic *** experimentally identify D-peptide binders to interesting therapeutic targets,so-called mirror-image phage display is typically performed,whereby the target is synthesized in D-form and L-peptide binders are screened as in conventional phage *** technique is extremely powerful,but it requires the synthesis of the target in D-form,which is challenging for large *** we present finDr,a novel web server for the computational identification and optimization of D-peptide ligands to any protein structure(https://***/).finDr performs molecular docking to virtually screen a library of helical 12-mer peptides extracted from the RCSB Protein Data Bank(PDB)for their ability to bind to the *** a separate,heuristic approach to search the chemical space of 12-mer peptides,finDr executes a customizable evolutionary algorithm(EA)for the de novo identification or optimization of D-peptide *** a proof of principle,we demonstrate the validity of our approach to predict optimal binders to the pharmacologically relevant target phenol soluble modulin alpha 3(PSMα3),a toxin of methicillin-resistant Staphylococcus aureus(MRSA).We validate the predictions using in vitro binding assays,supporting the success of this *** to conventional methods,finDr provides a low cost and easy-to-use alternative for the identification of D-peptide ligands against protein targets of choice without size *** believe finDr will facilitate D-peptide discovery with implications in biotechnology and biomedicine.

关键词： D-peptide Web server Evolutionary algorithm Peptide design Molecular docking Mirror-image phage display

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动态应力触发的余震

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世界地震译丛 2002年第3期33卷 18-22页

作者： D．kilb J．Gomberg P．Bodin 万永革杨智娴不详中国地震局防灾技术高等专科学校

通常认为断层永久位移产生的小“静态”应力变化可以改变附近断层上发生地震的可能性或者说可触发地震(Harris,1998)。许多近场的触发地震,特别是触发余震的研究(Dieterich,1994;Toda,et al,1998;King,et al,1994),将这种静态变化视为... 详细信息

通常认为断层永久位移产生的小“静态”应力变化可以改变附近断层上发生地震的可能性或者说可触发地震(Harris,1998)。许多近场的触发地震,特别是触发余震的研究(Dieterich,1994;Toda,et al,1998;King,et al,1994),将这种静态变化视为触发因素,并认为它与断层上负载的变化是等价的(Toda,et al,1998;King,et al,1994;Jaume and sykes,1992;Harris and Simpson,1992)。这里我们报道矩震级Mw=7.3的兰德斯地震的余震图象与应力变化的比较,不仅与静态应力做对比,而且与地震波传递的瞬态、振荡应力变化(即,“动态”应力)做对比。动态应力不会永久地改变加载情况,仅能通过改变断层区的力学状态或性质来触发地震。这些被动态弱化的断层在地震波通过后可能破裂,甚至能导致如果没有动态应力就不会发生的地震。我们发现余震和动态应力图象都具有类似的不对称性,动态应力来自于破裂的传播,而静态应力变化没有这种不对称性。先前的研究表明,动态应力在远距离处可促使破裂(Anderson,et al,1994;Gomberg and Bodin,1994;Gomberg,1996;Gomberg and Davis,1996;Hill,et al,1993,1995),然而本文表明在近处也是如此。

关键词：断层应力变化触发地震地震还辐射地震波

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