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Chinese Science Bulletin 2009年第2期54卷 212-219页

作者： DU HuiZhi li miaoyu YANG Pin Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education Institute of Molecular Science Shanxi University Taiyuan 030006 China

3-benzidino-5-methyl-6-phenylpyridazine (BMP) inhibited electric eel acetylcholinesterase (AChE), with IC50 being 0.58 μmol·L-1. As an AChE inhibitor, the effects of BMP on delayed rectifier potassium current (IK(DR)) and transient outward potassium current (IK(A)) in acutely isolated rat hippocampal pyramidal neurons were studied using the whole cell patch-clamp technique. BMP (0.1―50 μmol·L-1) inhibited IK(DR) and IK(A) in a concentration-dependent and voltage-independent manner. The IC50 value for the blocking action of BMP on IK(DR) and IK(A) was calculated to be 2.92 and 2.11 μmol·L-1, respectively. At the concentration of 10 μmol·L-1, BMP shifted the activation curve of IK(DR) to negative potential by 8.85 mV. Meanwhile, at the concentration of 10 μmol·L-1, BMP also shifted the activation and the steady-state inactivation curve of IK(A) to negative potential by 5.82 mV and 10.02 mV, respectively. In conclusion, BMP potently inhibits IK(DR) and IK(A) in rat hippocampal pyramidal neurons, which may contribute to restore the damaged central nervous system.

关键词：乙酰胆碱脂酶 BMP ACHE 活化激活

An Acetylcholinesterase Biosensor Based on Graphene/ Polyaniline Composite Film for Detection of Pesticides

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Chinese Journal of Chemistry 2016年第1期34卷 82-88页

作者： Yanping li Yuyu Zhang Gaoyi Han Yaoming Xiao miaoyu li Wen Zhou Institute of Molecular Science Key Laboratory of Shanxi Province for Energy Conversion and Storage Materials Taiyuan Shanxi 030006 China

This work reports a sensitive amperometric biosensor for organophosphate pesticides （OPs） fabricated through modifying glassy carbon electrode with acetylcholinesterase （ACHE） immobilized on graphene/polyaniline （G/PANI） composite film. The obtained G/PANI composite films show large specific area, high conductivity, good biocompatibility, and fast redox properties and have perfect layered and encapsulated structures. The as-prepared biosensor shows high affinity to acetylthiocholine （ATC1） with a Micbaelis-Menten constant value of 0.20 mmol/L. Furthermore, based on the inhibition of the enzymatic activity （immobilized ACHE） caused by the model compound of carbaryl （one kind of pesticides）, it is found that the inhibition activity of carbaryl is proportional to its concen- tration ranging from 38 to 194 ngomL-1. The developed biosensor shows a detection limit of 20 ngomL-1 （S/N~ 3） for OPs detection and exhibits good performance such as good reproducibility and acceptable stability, which makes it possible to provide a new and promising tool for the analysis of enzyme inhibitors.

关键词： acetylcholinesterase organophosphorus pesticides graphene/polyaniline biosensor

Isolation and characterization of AHL-degrading bacteria from fish and pond sediment

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Journal of Oceanology and limnology 2019年第4期37卷 1460-1467页

作者： li miaoyu XI Bingwen QIN Ting CHEN Kai XIE Jun Wuxi Fisheries College Nanjing Agricultural University Wuxi 214081 China Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization Ministry of Agriculture Freshwater Fisheries Research Center Chinese Academy of Fishery Sciences Wuxi 214081 China

Quorum sensing (QS) disruption is considered as a potential alternative strategy to combat bacterial diseases in aquaculture. In this study, we isolated and identified bacteria degrading QS molecules from pond sediment and fish intestine. A total of 132 strains were obtained in the enrichment culture, of which two strains were identified as Enterobacter sp. f003 and Staphylococcus sp. sw120, being isolated from the fish intestine and pond sediment, respectively. We found that strains f003 and sw120 could degrade acyl-homoserine lactones (AHLs) and cause no hemolysis of sheep red blood cells. The AHL lactonase (aiiA) homologous gene in the two strains was detected in PCR amplification and the high-degrading activity to N-hexanoyl-L-homoserine lactone (C6-HSL) and AHLs secreted from pathogenic Aeromonas hydrophila was assessed. Meanwhile, the artificial infection of cyprinid Carassius auratus gibelio with intraperitoneal injection showed that the two strains were avirulent. Therefore, the obtained indigenous bacteria are candidate probiotics against pathogenic A. hydrophila in aquaculture.

关键词： quorum sensing (QS) Aeromonas hydrophila probiotic Enterobacter Staphylococcus

Capacitive Performances of Reduced Graphene Oxide Hydrogel Prepared by Using Sodium Hypophosphite as Reducer

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Chinese Journal of Chemistry 2016年第1期34卷 89-97页

作者： Cuixian liu Gaoyi Han Yunzhen Chang Yaoming Xiao Yanping li miaoyu li Haihan Zhou The Institute of Molecular Science Key Laboratory of Shanxi Province for Energy Conversion and Storage Materials Taiyuan Shanxi 030006 China

The hydrogels of reduced graphene oxide （rGOHGs） have been synthesized through treating GO with various concentrations of sodium hypophosphite （NaH2PO2） under hydrothermal condition. The results reveal that the structure of rGOHGs changes along with the increase of the concentration of NaH2PO2. The rGO sheets prepared with low concentration of NaH2PO2 exhibit more crimples and larger specific surface area than those prepared with high concentration of NaH2PO2. The measurements of the capacitive performances of rGOHGs reveal that the rGOHGs prepared with low concentration of NaH2PO2 possess larger specific capacitance （Cs） than that prepared with high concentration of NaH2PO2 at low cyclic voltammetry scan rates. However, the rGOHGs prepared with high concentration of NaH2PO2 possess larger Cs at high scan rates, more stability and better high-rate capability than those prepared at low concentration. It is believed that the structures and properties of rGOHGs can be adjusted by controlling the concentration of NaH2PO2 according to the requirement.

关键词： graphene hydrogels NaH2PO2 electrochemical capacitors hydrothermal

Sulfonated Graphene Synthesized via a Green Route and Its Capacitive Properties

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Chinese Journal of Chemistry 2016年第1期34卷 98-106页

作者： Wen Zhou Gaoyi Han Yaoming Xiao Yunzhen Chang miaoyu li Yuyu Zhang Institute of Molecular Science Key Laboratory of Shanxi Province for Energy Conversion and Storage Materials Shanxi University Taiyuan Shanxi 030006 China

An environmentally friendly approach is presented to synthesize sulfonated reduced graphene oxide （S-rGO） by using L-ascorbic acid （L-AA） and aryl diazonium salt of sulfanilic acid. The preparation conditions have been optimized in order to obtain isolated and conductive S-rGO, and the products have been characterized by Ultraviolet-Visible spectroscopy, Fourier transformed infrared spectroscopy, Raman, X-ray photoelectron spectroscopy and X-ray powder diffraction and electrochemical methods. The results show that the S-rGO sheets possess excellent water-solubility and high electrical conductivity, which implies that the oxygen-containing functional groups have been removed and conjugated sp2 network has been restored. What＇s more, the electrochemical measurements reveal that the capacitive performance of the S-rGO has been improved compared with the graphene oxide （GO） and the reduced graphene oxide （rGO, reduced by L-AA）. The optimum S-rGO exhibits a specific capacitance of 205 F·g^-1 and good cycling stability （3.9% decreasing after 10000 cycles）, which are better than those for graphene oxide （109 F·g^-1 and decreasing 6.6% after 10000 cycles） and rGO （139 F·g^-1 and decreasing ll.3% after 10000 cycling）. This approach proves a new route to improve the capacitive properties of rGO.

关键词： sulfonated graphene L-ascorbic acid soluble graphene capacitive property

Facile Synthesis of Pd-Ni Nanoparticles on Reduced Graphene Oxide under Microwave Irradiation for Formic Acid Oxidation

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Chinese Journal of Chemistry 2017年第9期35卷 1405-1410页

作者： miaoyu li Ruiqin liu Gaoyi Han Yanni Tian Yunzhen Chang Yaoming Xiao Institute of Molecular Science Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province Shanxi University Taiyuan Shanxi 030006 China

Pd and PdxNi nanoparticles have been supported on reduced graphene oxide （Pd/rGO and PdxNi/rGO） by using the microwave-assisted heating method in glycol. The morphology, composition and electrochemical performance have been characterized by TEM, XRD, XPS and electrochemical methods. The XRD and XPS results show that there are no PdNi alloy particles formed in PdxNi/rGO and the composites exist mostly in the form of Pd^0 and NiOOH species. The electrochemical results reveal that PdxNi/rGO synthesized from the feeding source of Pd and Ni with an atomic ratio of 4 ： 1 exhibits higher activity, better stability and smaller electron transfer resistance to- ward formic acid electro-oxidation compared with commercial Pd/C, Pd/rGO and other PdxNi/rGO samples. The excellent electrocatalytic performance indicates that the addition of appropriate amount of Ni can greatly enhance the activity and stability of Pd catalysts for formic acid oxidation.

关键词： graphene palladium palladium-nickel formic acid electro-oxidation

Surface decoration prompting the decontamination of active sites in high-temperature proton exchange membrane fuel cells

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Chinese Chemical Letters 2024年第11期35卷 549-553页

作者： Jiaqi lin Pupu Yang Yimin Jiang Shiqian Du Dongcai Zhang Gen Huang Jinbo Wang Jun Wang Qie liu miaoyu li Yujie Wu Peng Long Yangyang Zhou li Tao Shuangyin Wang Research Institute of Hunan University in Chongqing Chongqing 401120China College of Chemistry and Chemical Engineering Jiangxi Science and Technology Normal UniversityNanchang 330013China KBC Hydrogen Energy Technology Co. Ltd.Yiyang 413100China

In electrochemical energy devices,the operating conditions always exert enormous influence on electrocatalysts'*** acid(PA),acted as the proton carriers,can be adsorbed on Pt surface,block active sites and affect the electronic structure of Pt unfavorably,which severely restricts the performance of high-temperature proton exchange membrane fuel cells(HT-PEMFCs).Herein,simply basic organic compounds,such as dicyandiamide(DCD),melamine(Mel)and cyanuric acid(CA),are decorated on Pt surface(DCD-Pt/C,Mel-Pt/C and CA-Pt/C)to induce the adsorption transfer of proton *** decoration can not only inject electrons to Pt and enhance oxygen reduction reaction(ORR)activity but also can induce PA to transfer from Pt surface to organic compounds,decontaminating active *** addition,the organic compounds with the larger conjugated system and the smaller electronegativity of ligating atoms would have a greater interaction with Pt,causing a larger decoration amount on Pt surface,which leads to more excellent ORR activity and resistance to PA blockage ***,MelPt/C shows a peak power density of 629 mW/cm^(2),exceeding commercial Pt/C(437 mW/cm^(2)),DCD-Pt/C(539 m W/cm^(2))and CA-Pt/C(511 mW/cm^(2))with the same loading.

关键词： High-temperature proton exchange membrane fuel cells Decontamination of active sites Oxygen reduction reaction Surface decoration Adsorption transfer

用于多光谱和高光谱图像融合的联合自注意力Transformer

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中国图象图形学报 2023年第12期28卷 3922-3934页

作者：李妙宇付莹北京理工大学计算机学院北京100089

目的将高光谱图像和多光谱图像进行融合,可以获得具有高空间分辨率和高光谱分辨率的光谱图像,提升光谱图像的质量。现有的基于深度学习的融合方法虽然表现良好,但缺乏对多源图像特征中光谱和空间长距离依赖关系的联合探索。为有效利用... 详细信息

目的将高光谱图像和多光谱图像进行融合,可以获得具有高空间分辨率和高光谱分辨率的光谱图像,提升光谱图像的质量。现有的基于深度学习的融合方法虽然表现良好,但缺乏对多源图像特征中光谱和空间长距离依赖关系的联合探索。为有效利用图像的光谱相关性和空间相似性,提出一种联合自注意力的Transformer网络来实现多光谱和高光谱图像融合超分辨。方法首先利用联合自注意力模块,通过光谱注意力机制提取高光谱图像的光谱相关性特征,通过空间注意力机制提取多光谱图像的空间相似性特征,将获得的联合相似性特征用于指导高光谱图像和多光谱图像的融合;随后,将得到的融合特征输入到基于滑动窗口的残差Transformer深度网络中,探索融合特征的长距离依赖信息,学习深度先验融合知识;最后,特征通过卷积层映射为高空间分辨率的高光谱图像。结果在CAVE和Harvard光谱数据集上分别进行了不同采样倍率下的实验,实验结果表明,与对比方法相比,本文方法从定量指标和视觉效果上,都取得了更好的效果。本文方法相较于性能第二的方法EDBIN(enhanced deep blind iterative network),在CAVE数据集上峰值信噪比提高了0.5 dB,在Harvard数据集上峰值信噪比提高了0.6 dB。结论本文方法能够更好地融合光谱信息和空间信息,显著提升高光谱融合超分图像的质量。

关键词：超分辨率高光谱图像多光谱图像联合自注意力 Transformer 融合算法

黄土高原地区生态系统碳储量空间分布及其影响因素

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生态学报 2021年第17期41卷 6786-6799页

作者：李妙宇上官周平邓蕾中国科学院水利部水土保持研究所黄土高原土壤侵蚀与旱地农业国家重点实验室杨凌712100 中国科学院大学北京100049 西北农林科技大学水土保持研究所杨凌712100

准确估算生态系统碳储量,探明其空间分布及其影响因素对区域生态管理具有重要意义,但黄土高原地区碳储量现状、空间格局及其驱动因素尚不清楚。选择黄土高原地区森林(包括乔木林和灌木林),草地和农田生态系统为对象,基于大量实测样点通... 详细信息

准确估算生态系统碳储量,探明其空间分布及其影响因素对区域生态管理具有重要意义,但黄土高原地区碳储量现状、空间格局及其驱动因素尚不清楚。选择黄土高原地区森林(包括乔木林和灌木林),草地和农田生态系统为对象,基于大量实测样点通过克里金插值和地统计方法,评估了三种生态系统地上生物量碳密度、地下生物量碳密度和0—100 cm土壤有机碳密度空间分布,并通过路径分析探讨了各碳库的主要影响因素。结果表明:黄土高原地区约占全国总面积的6.7%,其生态系统总碳储量约为2.29 Pg,仅占我国生态系统碳储量的2.3%。生态系统各碳库中,地上生物量碳储量、地下生物量碳储量、土壤有机碳储量分别为0.44、0.32和1.52 Pg;森林、草地、农田(仅指土壤)生态系统碳储量分别为0.98、1.09和0.21 Pg。气候(年均温度、年均降水)、海拔、坡度、土壤质地(砂粒、粉粒、粘粒含量)、植被覆盖状况(用NDVI表示)等因子可解释地上生物量碳密度、地下生物量碳密度、农田土壤有机碳密度空间变异的12%、8%和32%,其中,年均降水、海拔、粘粒含量是黄土高原地区生态系统碳储量空间格局的主要影响因素。本研究表明,由于黄土高原地区独特的气候、地形和土壤条件,其生态系统虽然具有较大的碳储量,但是低于我国生态系统碳储量的平均水平。

关键词：黄土高原碳密度碳储量空间格局生态系统

Effects of Bifidobacteria bifidum strains on 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced acute colitis and its potential mechanism

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Food Bioscience 2023年第2期52卷 458-467页

作者： Dingwu Qu Saisai Feng miaoyu li Leilei Yu Fengwei Tian Hao Zhang Wei Chen Qixiao Zhai State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxiJiangsu214122China School of Food Science and Technology Jiangnan UniversityWuxiJiangsu214122China National Engineering Research Center for Functional Food Jiangnan UniversityWuxiJiangsu214122China Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi BranchChina

Microbiota-based therapies for improving symptoms of Crohn’s disease(CD)are receiving increasing *** and second-generation probiotics can potentially prevent this ***,the therapeutic action and underlying molecular mechanisms of probiotics remain largely *** screening of probiotics is also *** this study,we screened eight *** strains with CD-relieving potential using a 2,4,6-trinitrobenzene sulfonic acid(TNBS)colitis mouse *** also observed that *** CJ238 could prevent but not cure the colitis-symptoms(weight loss,colon shortening and connective tissue hyperplasia)in mice *** 16S rRNA sequencing data showed that supplementation with *** did not alter the composition of the intestinal *** CJ238 administration also provided full protection against acute colitis in pseudo germ-free ***,we infered that the protective effect of *** strains does not depend on their interaction with the gut ***,heat-killed(95℃ for 20min)*** cells(109 CFU/mouse)also protected mice against acute ***-vitro adhesion and immunomodulatory activities of the *** strains were *** with the in-vivo results,the anti-colitis effect of *** was positively associated with its strong anti-inflammatory and adhesion capacity *** study provides insights into the in-vitro screening and clinical application of probiotics in CD prevention.

关键词： Bifidobacteria bifidum TNBS colitis Gut microbiota Immunomodulatory Adhesion