An Acetylcholinesterase Biosensor Based on Graphene/ Polyaniline Composite Film for Detection of Pesticides
An Acetylcholinesterase Biosensor Based on Graphene/ Polyaniline Composite Film for Detection of Pesticides作者机构：Institute of Molecular Science Key Laboratory of Shanxi Province for Energy Conversion and Storage Materials Taiyuan Shanxi 030006 China
出 版 物：《Chinese Journal of Chemistry》 (中国化学（英文版）)
年 卷 期：2016年第34卷第1期
页 面：82-88页
核心收录：
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 081701[工学-化学工程] 0703[理学-化学]
基 金：Acknowledgement The authors thank the National Natural Science Foundation of China (Nos. 21407100 21574076 61504076 and 21501113) the Natural Science Foundation of Shanxi Province (No. 2014011016-1) and the Program for the Top Young and Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi (No. 02035290 1014)
主 题：acetylcholinesterase organophosphorus pesticides graphene/polyaniline biosensor
摘 要：This work reports a sensitive amperometric biosensor for organophosphate pesticides （OPs） fabricated through modifying glassy carbon electrode with acetylcholinesterase （ACHE） immobilized on graphene/polyaniline （G/PANI） composite film. The obtained G/PANI composite films show large specific area, high conductivity, good biocompatibility, and fast redox properties and have perfect layered and encapsulated structures. The as-prepared biosensor shows high affinity to acetylthiocholine （ATC1） with a Micbaelis-Menten constant value of 0.20 mmol/L. Furthermore, based on the inhibition of the enzymatic activity （immobilized ACHE） caused by the model compound of carbaryl （one kind of pesticides）, it is found that the inhibition activity of carbaryl is proportional to its concen- tration ranging from 38 to 194 ngomL-1. The developed biosensor shows a detection limit of 20 ngomL-1 （S/N~ 3） for OPs detection and exhibits good performance such as good reproducibility and acceptable stability, which makes it possible to provide a new and promising tool for the analysis of enzyme inhibitors.
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